Hot start PCR || principle and usage

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  • Опубліковано 27 кві 2021
  • This video describes the principle behind hot start PCR.Hot Start PCR is a technique that inhibits Taq polymerase activity or the incorporation of modified dNTPs during reaction set up until a heat activation step occurs. Hot Start PCR allows for reaction set up at room temperature without non-specific amplification and primer dimer formation.

КОМЕНТАРІ • 26

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    @user-dj1wu7ye5f 2 роки тому +6

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  • @Mr.Neelesh_Saha_
    @Mr.Neelesh_Saha_ 3 роки тому +6

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  • @adhoccerswings
    @adhoccerswings 2 роки тому +1

    Nice! I'm pretty sure I have never heard of this during my studies

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому +1

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  • @yokoshiko9427
    @yokoshiko9427 2 роки тому +2

    Very useful and helpful 👌thank you sir😇

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому +1

      Please share my channel link with your friends and help me to reach big audience

    • @yokoshiko9427
      @yokoshiko9427 2 роки тому

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  • @mahnazmehrabi7626
    @mahnazmehrabi7626 2 роки тому +1

    Thanks

  • @barnalisinha4705
    @barnalisinha4705 3 роки тому +2

    Thank you so much ☺️

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому +1

      Please share my channel link with your friends and help me to reach big audience

    • @barnalisinha4705
      @barnalisinha4705 3 роки тому +1

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      @animatedbiologywitharpan  3 роки тому +1

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      @barnalisinha4705 3 роки тому +2

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    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому +1

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  • @jayalekshmir7276
    @jayalekshmir7276 6 місяців тому +1

    Sir how you create this vedio...which app is used? Plz reply

  • @sandraerdmann6003
    @sandraerdmann6003 8 місяців тому +1

    would this increase the chance of degradation of DNA in the mix?

  • @lennylink6574
    @lennylink6574 Рік тому

    What is the "chemical X" exactly ?

    • @amiygupta1880
      @amiygupta1880 Рік тому

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