You wouldn't know definitively without standards, but it's typical in HPLC methods that the first few minutes will be chemicals that passed through the HPLC column without absorbing to the column. Chances are they designed the HPLC method so that happened. The 'other ingredients' are usually called 'excipients' in the pharmaceutical industry, and they may be proprietary mixtures of stabilizers/preservatives/flavor additives/ etc...
I use one of these machines everyday, and don't know much about them. (neither does any of the other employees tho) This was somewhat informative. I've been self learning chromatography ever since I started this job. Kinda hard to do tho, as I'm not a chemistry buff...
hi everybody, i'm from Vietnam, my daughter want to research this HPLC video, but we can't catch the speed of the reader. Can anybody help me to give my daughter the subtitile to understand what this video talk about ? please help us ... many thanks
@282sanjay Yes you can use HPLC to measure these. However, I work in a hospital and we measure T3, T4 TSH and calcitonin via immunoassay. Its a much cheaper, faster and more sensitive method. You can obtain results within 30minutes, compared to HPLC which takes atleast a few hours and more working time to extract
The presentation was great, and the lab work too. But there were some glitches. The chart didn't show perfectly defined peaks for the strong substance. Then, the needle of autosampler was not shielded.
The quality of those peaks, tho... I do LCMS data review and you have no idea how happy I get when I see perfect chromatography. If the peak is messed up I have to give it a makeover with the software and possibly reinject the whole damn batch. Not a fun time.
Very amazing video nice and simple , but why u didn't add the applications of the HPLC it would be of great important to those who use it in pharmaceutical industry ,,
Great video, simple explanation. Was hoping for more of an explanation for column selection, mobile phase composition, and other factors influencing compound separation. Would love to see a video for spectrofluorimetry.
the liquid phase is polar. h2o is a polar substance which means that things that are polar will more easily dissolve in it. that means that the more polar substances will have an easier time following the water. don't forget that in solutions like dissolves like.
exactly! Because the stationary phase is non-polar, the non-polar component had a greater affinity to it. In other words, it would spend more time being attracted to the stationary phase in comparison with the other component.
Hi, This is likely because, as implied in the video at 3:41, the light needs to be set at a wavelength that is absorbed by all the components to be separated.
You tube University does it again... thank you
Very informative, simple and clear..specially for students..thanks for uploading
British people are good at explaining things.
You wouldn't know definitively without standards, but it's typical in HPLC methods that the first few minutes will be chemicals that passed through the HPLC column without absorbing to the column. Chances are they designed the HPLC method so that happened. The 'other ingredients' are usually called 'excipients' in the pharmaceutical industry, and they may be proprietary mixtures of stabilizers/preservatives/flavor additives/ etc...
I use one of these machines everyday, and don't know much about them. (neither does any of the other employees tho) This was somewhat informative. I've been self learning chromatography ever since I started this job. Kinda hard to do tho, as I'm not a chemistry buff...
hi everybody, i'm from Vietnam, my daughter want to research this HPLC video, but we can't catch the speed of the reader. Can anybody help me to give my daughter the subtitile to understand what this video talk about ? please help us ... many thanks
It's a quite beneficial video. Thanks to all contributors.
Yes, but only in aid of the separation. Then you would do 1H-NMR/13C-NMR or mass spect for identification.
cheers
Thank u so much for making this video I did not see hplc operates before this video
@282sanjay
Yes you can use HPLC to measure these. However, I work in a hospital and we measure T3, T4 TSH and calcitonin via immunoassay. Its a much cheaper, faster and more sensitive method. You can obtain results within 30minutes, compared to HPLC which takes atleast a few hours and more working time to extract
The presentation was great, and the lab work too. But there were some glitches.
The chart didn't show perfectly defined peaks for the strong substance. Then, the needle of autosampler was not shielded.
thank you, is very academic for people that want learning HPLC
Acetonitrile is the primary NIST and IUPAC name, and I've never seen it bottled commercially other than Acetonitrile.
The quality of those peaks, tho... I do LCMS data review and you have no idea how happy I get when I see perfect chromatography.
If the peak is messed up I have to give it a makeover with the software and possibly reinject the whole damn batch. Not a fun time.
Very amazing video nice and simple , but why u didn't add the applications of the HPLC it would be of great important to those who use it in pharmaceutical industry ,,
@saurabhCherished I would suggest normal chromatographic columns, if the product is well known, an affinity column might be aqble to do the job.
Great video, simple explanation. Was hoping for more of an explanation for column selection, mobile phase composition, and other factors influencing compound separation.
Would love to see a video for spectrofluorimetry.
Very simple explantion! Very helpful!
This is a brief but good lecture.
brillant work,very informative and thanks
nice vidio this is very usefull for clear basics about hplc
Its a Jasco HPLC system, do a quick google search for "jasco HPLC" it should yeild some results
Thanks! this really helped. I wonder how they use this along with MS.
Yes, for quantitation or identification.
Thx, helps me for my test in 2 days :)Greetings from germany!
great for university students in the first and second grades
The video appears to be quite useful... thanx for that
Very so helpful, thank you so much!
ethanenitrile that's the correct way of saying it acetonitrile is old terminology although many books are still using it unfortunately
very good illustration !!
can anyone explain why the more polar component came out first? is it because the stationary phase is non- polar?
Ivan Torres you got it
the liquid phase is polar. h2o is a polar substance which means that things that are polar will more easily dissolve in it. that means that the more polar substances will have an easier time following the water. don't forget that in solutions like dissolves like.
Ivan Torres Yup.
Ivan Torres yeah and affinity too
exactly! Because the stationary phase is non-polar, the non-polar component had a greater affinity to it. In other words, it would spend more time being attracted to the stationary phase in comparison with the other component.
thnx helped a lot with my chem cswrk!
Brilliant video. Very clear.
Coooool can’t wait for tomm!
Can HPLC be used to extract the individual ingredients from the injected solutions?
very useful for proteomics studies.
no i disagree all of what she said is wrong, she got her a and her bs mixed up
This is what i do for work!
Gud 4 u
Nice. Do you work with the machine itself or do you do data review?
Jah Kah me too. But this equipment is very old...
I need to determine residual sugars in the shake flask cultures. I need to prepare culture supernatant for analysis by HPLC. How can I do that?
oh god you simply opened the column?!?! is it recommend??? first time I could see the powder of silica from the coulmn...
can HPLC be used for finding the chemical content of fuel oils (bio-diesel)....?????
ethane nitrile... also known as acetonitrile. If it says ACN on the bottle, why not use the more common name :) good video though.
because British
Because those who know - know? To those who don’t know, it really doesn’t make much difference.
@Yibitech
So which technology is used for large quantities?
anybody know whre to buy this product?can gime me web site or company supply this product.thanks.
This are very interesting importance instruments
What is the price of a unit like this?
Is there any recommendation for effective HSCCC (high speed counter current chromatography) ?
what would a home set up cost?
really a nice one for d beginners
Excellent, very informative
Thanks for posting! Very Informative!
Thank you for this video.
Very very good! Thanks....
hey really great video guys, keep up the good work ladzzzzzzzzzz
Great video! Thanks
Good explanation...
great video that we want
its really great
How to use in only q c
manual injection, dang that's an old instrument
***** 30 years ago, we used to have to pack our own columns!
for each run?
I'm more concerned with consistency between column to column. lol.
good video....................thanks
Tomorrow we have exam in HPLC , chemical science Baghdad university by Dr.yahia 😍😂
Well explained tutorial, thank you.
I have an essay ... hplc .. not sure how to start or structure it sooo much research out there 🙄
Great video
Thanks a lot ! very useful
very good !!
Thank you great video
thnks a lot. its really helpfull
Thanks for this video......good...
Thanks for the vid !
So nice
thank you. it was helpful
thank you very much.
amazing thank you
thanks for this video
thanks for uploading
awesome
amazing
thanku..
nice video,
cool video, makes more sense..i think!
Thanks ...
gooooooooooood and very nice
thank U ! That will help me :)
nice video
Thanks good video
agreed, visuals very clear
lifesaver
great
nice one
10years ago 😯😶
bravo bravo score
super
Useful for a lvels
anyone can explain me why do we use UV spectra to get a peak in HPLC method. Even though there is other spectrum such as IR, X-ray,..etc. ?
Hi,
This is likely because, as implied in the video at 3:41, the light needs to be set at a wavelength that is absorbed by all the components to be separated.
good
My Professor told me its about $30,000
nice vedio
In other words, if you have to ask...
biri bunu türkçeye çevirse muhteşem olur :)
70,000AU