Yes, ions need to have clear path from formation over deflection to detector. Rotation pump you see behind the instrument produce low vacuum after which is achieved high vacuum by using pumps like turbomolecular pumps or diffusion pumps. Rotation pumps then pump out particles from system - their output is directed to atmosphere over filters to avoid expelling possible harmful substances. Vacuum must be maintained constantly because even smallest introduction of foreign particles makes it worse.
MS is acronym of mass spectrometry in general or separate techniques of mass spectrometry, for me GCMS or LCMS means mostly chromatograph with one quadrupole MS, MS/MS is tandem mass spectrometer, mostly ion trap that can make even more than 2 separations, but there are other descriptions for example MS^n, qqqMS, TOFMS, many other techniques...
Okay, the magnetic deflection will separate whatever ion you want or need. I basically already knew that. Now I have the answer I needed for the IPG in the post collerating chamber. This is great news! Thank you.
excellent video, thank you very much for this, it has really cleared this up for me, i am an organic chemistry 2 student... thanks to you i have a much clearer understand regarding the readout. the narration is excellent btw.
5:02 it's not the mass that is shown on x-axis, it's the m/z ratio! Depending of type of ionization, molecule it sell it can often have same numeric value like molecular mass but it's not always the case!
So, functionally, what is the difference between using electric and magnetic fields to deflect the ions? Probably not going to be in my exam, but I'm very curious about a lot of things.
@wwwRSCorg I will change nothing in the video, neither the sound or logo. Only I add a subtitle file in italian. This idea born in me when I studied for entry in a PhD and I couldn't find anything in italian.
Does anyone know what is the use of having a magnetic field if an electric field is already present for deflection? Thomson used a magnetic field as well.
electric field is deflecting all ions through the same angle however magnetic field deflects them according to their mass/charge ratio. As we know that centripetal force created is because of the magnetic field applied in the plane prependicular to the direction of ions. (mv2/r = ZevB)
Hi, I understand that the true (non-relative) masses of atoms are calculated with a mass spectrometer. using this relationship: Centripetal Force = Force due to magnetic field(B) But i was wondering how masses of atoms were calculated before the mass spectrometers were invented.
Armani : Before mass spectrometers there were 3 ways to find the atomic masses : -- The stoichiometric law : weight 10g of pure magnesium, burn it in free air, weight the obtained MgO, it will be 16.6, and the number of moles is constant = 10/mass(Mg) = 16.6 / (mass(Mg)+mass(O)) -- The law of perfect gases : each molecule is a small ball, the temperature is the kinetic energy per ball, and by Newton's law at constant temperature and pressure then the number of molecules per volume doesn't depend on the mass of the balls, see en.wikipedia.org/wiki/Kinetic_theory_of_gases#Equilibrium_properties -- Law of Dulong-Petit : in a crystalised solid the temperature is that each atom is vibrating around its mean position, the kinetic energy is then mv^2 and at thermal equilibrium it is constant from one atom to the other, this is the temperature T = 2mv^2/3, thus the energy needed to increase the temperature of one Kelvin depends on the number of atoms not on their masses.
more useful than my teacher
Yes, ions need to have clear path from formation over deflection to detector. Rotation pump you see behind the instrument produce low vacuum after which is achieved high vacuum by using pumps like turbomolecular pumps or diffusion pumps. Rotation pumps then pump out particles from system - their output is directed to atmosphere over filters to avoid expelling possible harmful substances. Vacuum must be maintained constantly because even smallest introduction of foreign particles makes it worse.
This is really interesting and seems very professional. Thanks.
Awesome to see such large oldskool MS spectrometers. How times have changed.
Great video!! Effective way of teching undergraduated about analytical tools which they will soon need for their laboratory experiments.
nice one
MS is acronym of mass spectrometry in general or separate techniques of mass spectrometry, for me GCMS or LCMS means mostly chromatograph with one quadrupole MS, MS/MS is tandem mass spectrometer, mostly ion trap that can make even more than 2 separations, but there are other descriptions for example MS^n, qqqMS, TOFMS, many other techniques...
Okay, the magnetic deflection will separate whatever ion you want or need. I basically already knew that. Now I have the answer I needed for the IPG in the post collerating chamber. This is great news! Thank you.
Thanks for this - very useful for Year 12 Chemistry
excellent video, thank you very much for this, it has really cleared this up for me, i am an organic chemistry 2 student... thanks to you i have a much clearer understand regarding the readout. the narration is excellent btw.
5:02 it's not the mass that is shown on x-axis, it's the m/z ratio! Depending of type of ionization, molecule it sell it can often have same numeric value like molecular mass but it's not always the case!
So, functionally, what is the difference between using electric and magnetic fields to deflect the ions? Probably not going to be in my exam, but I'm very curious about a lot of things.
@wwwRSCorg I will change nothing in the video, neither the sound or logo. Only I add a subtitle file in italian. This idea born in me when I studied for entry in a PhD and I couldn't find anything in italian.
I may just pass analytical chemistry now. Thank you
Ahh, the VG AutoSpec. That was one beautiful instrument!
Can someone tell me why the air has to be pumped out of the spectrometer first? Is it to avoid the interference of other air molecules?
Great video
Thank you! Very helpful with these subtitles.
wow this is complicated... wrote this a year ago, I still think its complicated but I understand it now :-)
Can you please tell me the difference between MS and MS/MS ? are their two ionization source or two separators
Does anyone know what is the use of having a magnetic field if an electric field is already present for deflection? Thomson used a magnetic field as well.
electric field is deflecting all ions through the same angle however magnetic field deflects them according to their mass/charge ratio. As we know that centripetal force created is because of the magnetic field applied in the plane prependicular to the direction of ions. (mv2/r = ZevB)
Thanks Mobahil. Unfortunately, I still don't understand the point of having a magnetic field. Why does the mass/chargo ratio matter?
well how can you differentiate two molecules with different masses without magentic field? do u have any other possible ideas ?
Ahhh I gotcha. Okay ! Thank youuu. Just had my epiphany moment. Haha.
Parker Leigh you are welcome :)
Thanks this helped a lot with my homework! Bit complex for 12 year olds though.
Hold up! Why did you have to learn about this stuff?
I'm a a 16 yo at college, i'm just learning about this, wut?
Very well made video. Thanks from the US.
Can re-pubblic this video with italian subtitles?
@wwwRSCorg I have resource to do this if you allow me pubblic this video on my channel with subtitles write by me. Is it possible?
Hi, I understand that the true (non-relative) masses of atoms are calculated with a mass spectrometer. using this relationship: Centripetal Force = Force due to magnetic field(B)
But i was wondering how masses of atoms were calculated before the mass spectrometers were invented.
Arman Ashourlou .
Check out Avogadro and the mole. Basically carbon is assigned a mass of 12 and every this calculated from that.
Cat. Parametrization
Parametrization Can convert ELECTRIC To MAGNETIC fields And Vice versa
Armani
: Before mass spectrometers there were 3 ways to find the atomic masses :
-- The stoichiometric law : weight 10g of pure magnesium, burn it in free air, weight the obtained MgO, it will be 16.6, and the number of moles is constant = 10/mass(Mg) = 16.6 / (mass(Mg)+mass(O))
-- The law of perfect gases : each molecule is a small ball, the temperature is the kinetic energy per ball, and by Newton's law at constant temperature and pressure then the number of molecules per volume doesn't depend on the mass of the balls, see en.wikipedia.org/wiki/Kinetic_theory_of_gases#Equilibrium_properties
-- Law of Dulong-Petit : in a crystalised solid the temperature is that each atom is vibrating around its mean position, the kinetic energy is then mv^2 and at thermal equilibrium it is constant from one atom to the other, this is the temperature T = 2mv^2/3, thus the energy needed to increase the temperature of one Kelvin depends on the number of atoms not on their masses.
Who is the manufacturer for this specific MS?
Very Precise video.Please also prepare more detailed videos on functioning and working of each component of ICP-MS
Waters Instruments. It is called the autospec
I must... learn.
I saw samekind of the sector MS but with more of different apparatuses and 2 extra pumps =)
Great video, but m/z at 107 was assigned for the wrong peak
your clips are informative. fantastic job
Excellent resource for the novice
Great video! Thank you!
VG EBE, have seen once in Bielefeld Germany
Thanks a lot U`ve explained it well
Good video, very helpful in my study.
why this technology is not used for uranium enrichment
Naeemullah Khan it used to be. But after invention of centrifuge everyone uses that now.
@krazzyrkb yes
Thanks !
@imamnalog z= charge on the magnet
my college teacher said he knows the guy at the start...
woah..most interesting
Life saver, cheers buddy!
This is really interesting and seems very professional. Thanks
thanks a lot for the video
Thank you.
Very informative
Merci c'est parfait
Mass spectrometry for colleges? They don't teach this stuff till you are an undergraduate.
Interessante
thank you so much.
thanx for the video!! I very appreciate it !;)
thanks! very helpful
thank you so much for dis vedio
Thank you :)
thanx!
thnks dudes
Thnkxxxx
Sweet Chemistry!!!
nice
Good video, but the sound in this video is really low making me hard to listen.
Why am I taking this in the 11th grade of school!
Come ti chiami?
Voodoyzans Mete Education sou nous min Maman Sorciere's
Henry Truong .. more like:
OhhhhhOMGMSMSMSMSM
:-}
Subtitles.
man science girls in labcoats...A+
bad.
bad
it's not even cost efficient.
So why dont you invent something else
+Alex Vincent bad and not even cost efficient.
thanks for the video!! I appreciate it very much! ;)