Immunofluorescence (Direct and Indirect)

[@briancannon4607]

3:14 - 4:40 compares direct and indirect immunofluorescence.

[@argumentumadbaculum]

Sajid S, you are a great teacher, I thoroughly enjoy the clarity in your video

[@ruggedbypowell]

man saved my ass even after 4 years of making the video! good work fam

[@sciencenerd752]

thank you :) i have an exam tomorrow and this is really helpful. :)

[@arunasrivatsa3914]

I have today :(

[@anomitasingha3106]

Same today🤕

[@artbyazra5117]

Same ,my paper is tomorrow

[@cholofelomalunga6744]

Nice presentation, we use immunoflourescence in diagnostic virology, where we use mouse monoclonal antibodies to bind to an antigen in a patient's specimen and then a goat labeled antimouse antibody binds to the antigen-antibody complex and a positive cell stains bright green due to flourescein isothiocynate staining, we also do direct immunoflorescence for quite a number of viruses.

[@sajidmicrobiology]

Thanx brother

[@noorulain7302]

Thanks for the nice information as well.

[@dina3630]

This channel is a treasure ❤

[@shantanusi6304]

It's the best video on immunofluorescence🎉
Thanks Sajid Sir

[@fazalrahimbatch3regno314]

More easy and more perfect for us. Thank you sir so much 🥰🥰

[@noorulain7302]

Very well explained......... thumbs up bravo

[@Manikandanmgn]

Concepts r delivered Crystal clear manner sir

[@jingtiankumari4747]

What a perfect explanation... 👏👏👏👏Thank you

[@LovePreet-wz7bf]

Very well explained .....Thank you sir

[@fazilfayaz6557]

Nicely explained

[@soundaryank545]

Nice explanation 👏👏

[@vikas_frvr]

awesome...keep it up

[@urva-til-vosqa117]

Great explanation 👍

[@ArchanaSingh-zi7kf]

Good explanation 🎉🎉

[@ashlyhan8858]

Thank you for such informative video, helped a lot.Looking forward for more of 'em.❤

[@mehakkhan92]

Thank you so much for this.

[@sitararoyalthanneeru3933]

Simple and clear 🎉

[@linakheyar738]

Indian people i respect you 🤝you are everywhere know everything i m proud of you ❤

[@nabanitachakraborty5228]

Amazing explanation

[@mirzasikandar1680]

Wonderfull Explanation

[@khanmuhammad5149]

Really a great way. Thanks a lot.

[@ilayRA100]

Great explanation! Thanks!! :)

[@dipalirajput6106]

I have a question, in procedure antibody binds with specific part of the cell but in direct and indirect method antibody binds with antigen, why?

[@karenjoybuma-a8764]

Thanks i find it helpful😊

[@aventadorgamer1575]

thank you very much it was tooo much helpful keep teaching us

[@IBTISSEMBENKADER]

thank you for the this detailed explanation

[@rrahimfridjaa3876]

booom bastic thank you so much you save my life

[@anishamn2172]

tku tku tku uuuuuuu.was very helpful😍😍

[@sajidmicrobiology]

Most welcome again

[@Its_Dazai_04]

If in indirect immunofluorescence, imAb binds with primary antibodies (which we want to diagonise) ,than why we even need the antigen?

[@sarasettou6717]

👏👏👏

[@giulialiberati1389]

Very well explained, thank you!

[@sajidmicrobiology]

Most wecome

[@sonykoutla3600]

Thank you so much sir.explained very neat..

[@sajidmicrobiology]

Most welcome

[@udhayabala8621]

Great

[@ankurbagde8270]

Fitc and tritc ke excitation and excitation wavelength kya hai?

[@sragdharaghosh3582]

Very well explained and the pictures were great!

[@bhavyasri3564]

Very clear
Thank

[@jackhammer40k_]

Thanks for your video. I have a question. Are the fluorescent antibodies synthetically produced proteins?

[@sajidmicrobiology]

Yes... antibodies are produced by hybridoma technology and then coupled with fluorescent dye

[@jackhammer40k_]

Thank you @@sajidmicrobiology

[@sciencequizzes6641]

Thank you so much!

[@dleprep]

Hello Dr. Thanks for your presentation. Could you kindly tell me the the doodle program you are using because I am a lecturer and want to use it in my presentations...Thanks in advance. Dr. Sameh

[@milesmorales2064]

you saved my life thx

[@tarteelmohammed3257]

Thank you. Very helpful!

[@aventadorgamer1575]

thank you again tomorrow is my exam you explained very well

[@afrozaga4464]

Very nice sir .. good voice quality. Which mic did u use sir

[@sajidmicrobiology]

Normal Samsung mobile earphones

[@afrozaga4464]

@@sajidmicrobiology very good voice sir. And brilliant lecture.!! Very useful video.👍

[@faruqsosanya]

Weldone

[@suryabhanyadav8497]

Thanku sir👍

[@ralucavalentina5028]

Thank you!!

[@yusufaljaber852]

Thanks man 👍

[@deryaozkocak3856]

Türkçe altyazı neden yok ya

[@akansha4575]

Thnkusomuch 😀

[@AnmolKhan812]

Paper m is se Kya question askt h?

[@jasminelee9849]

Hey just wondering what video editor/creator did you use for this video :)

[@shikhakushwaha2087]

👌👌👌

[@sajidmicrobiology]

Most welcome shika. You can help our channel by donating small amount (Rs 5 to 100) on paytm-8830061003

[@shikhakushwaha2087]

Okk I will try 😇

[@saratrials8632]

very useful

[@mariasanchezmenjivar8432]

Thanks Sajid

[@SLStudent95]

Thank you so much sir.

[@angelawong6777]

Can you please also explain how to quantitatively detect(antibody titer) the antibody level in serum samples using indirect IFA ?

[@M_5787]

Thankyou sir 😇

[@SNC-jq9kc]

How to calculate the Limit of Detection?

[@ladysilat1428]

Thanks a lot, but I have a question about this method if it is quantitatif or qualitatif or both them

[@tunerd1921]

Qualitative, you aren't getting exact numbers for the fluorescence in this method but rather you are visualizing either yes there is fluorescence and so the antigen of interest is present or no fluorescence meaning its not present. In the same way western blotting gives you a yes this protein is present and allows you to see a relative amount of the presence so if the blot is bigger there is more protein, here the more fluorescence observed the greater the protein/antigen presence but it is not quantitative.

[@jetpurabirbalbairwa6435]

Super

[@Swifty145]

Anyone else catch the 3D effect of the image at 2:55

[@freedomtv1505]

Love ya

[@novoamor7867]

thank you

[@BrajbihariRaj]

Thanks bro

[@pranavbhailume6213]

👍

[@PriyaDharshini-ru9he]

Thank u sir

[@hiraktailor1916]

Sir plz explain immunochromatography and hypersensitivity reactions

[@sajidmicrobiology]

Sure

[@varadharasun2826]

Easily understandable sir..need for Elisa too

[@rawyhjamal5215]

Thanks

[@shmema12]

thanks

[@minalisinha7441]

Can anyone help me with the history of indirect micro immunofluorescence

[@WildeTernence-f9r]

Hernandez Barbara Anderson Larry Jackson Gary

[@sujis142]

Thank you sir