How ion pair reagent works in RPLC?

Ion pair chromatography (IPC) is an effective reversed-phase liquid chromatographic (RPLC) technique for separation of organic ions and partly ionized organic analytes. The technique utilizes the same types of stationary phases and mobile phases as RPLC; the main characteristic for IPC is that an ion pair reagent is added to the mobile phase.

[@nigarfatima9301]

Sir can you please make video on response factor calculation for impurity

[@vinodsingh700]

Sir how to retain neutal compound

[@satnamom4667]

Sir, what should be chromatographic condition for amino acids

[@trendbizz2743]

Amino acid analysis or amino acid product rs

[@surenderchilka]

If retension time is less for polar compounds means,instead of using the Ion pair Reagents,make that polar molecule to slightly non polar molecule(by using the ionization formula decrease the % ionization by changing the pH).Let me the Answer please.

[@vinodsingh700]

Sir I think acidic at low pH having good retention and reverse for basic compunds . Please correct if I'm wrong?

[@pharmagrowthhub3083]

Yes Vinod, you are right

[@rohinikumar7065]

Sir please make a video on FDS study and it prblms how to over come prblms.