A Guide For Selection of Buffer for HPLC

[@annadevarasivaprasad3589]

I got a very well knowledge on Method development...tq u so much sir

[@milind0808]

Good explanation sir will help in method development a lot while selecting a buffer.

[@ardurinocreater8529]

Hi, Ample and crystal clear explanation. All the vedios are Bible of analytical chemistry. Thanks lot

[@rupanalam1369]

Your videos are very helpful.

[@shrutidixit499]

Lovely presentation sir... Thank you soo much

[@sohelinamdar437]

Nice explanation Sir..👌
It's great learning from your video's

[@jaykumarbhatt8841]

Very nice explanation 👌

[@rahulkulkarni3037]

Really useful in method development process

[@dhwarkeshgupta8465]

Fantastic explanation sir
Can I get the ppt which your we're used in this video.

[@jaykumarbhatt8841]

Thank sir.
You are genius

[@suranjanpaul16]

Dhanyawad *Gurudev*...

[@MohammadSalim-sd5vb]

Please let me know what are the reference you used?

[@nabeelpt8444]

Sir,
How will decide weight of salt for preparation 1L buffer?

[@piyu9853]

Thanks
Very helpful

[@SanjayThakur-rn4zs]

Nice explaination sir , thank for that.
My question is a molecule contain both basic and acidic functional group so retention of that molecule at acidic and basic pls explain

[@sairampolisetty331]

Hello sir please reply me for this doubt im having , how could you know pka value of particular buffer that is whats the pka of nah2po4 and what us exact pka value for na2hpo4 pls tell me sir

[@veerucration]

why we used in base pH in LC

[@maheshwaghmare8346]

Please make video of how to predict polar and non polar molecule

[@Vexins]

Look up The Organic Chemistry Tutor on UA-cam. Learn to draw Lewis structures and learn how to draw dipoles is the gist of it though.

[@talsaniyaraman6423]

Suggest book for method development on HPLC.

[@bhavanipodili2358]

Good explaination sir
I have one doubt from 2nd question
Why the acidic compounds are not ionized at lower pH?
Please explain

[@dils2678]

In acidic environment -COOH group dissociation will not takes place....thats y

[@subhanid6656]

Hi sir, thank you for the explanation. My doubght is,when we choose kh2po4, K2hpo4, etc

[@pharmagrowthhub3083]

K2HPO4 (dibasic potassium phosphate) is used for alkaline pH and KH2PO4 (monobasic potassium phosphate) for acidic pH

[@subhanid6656]

What happens if I use K2HPO4 for acidic pH and vice versa ..what change can I observe.pls explain sir

[@dils2678]

@@subhanid6656 Again we need to adjust more the pH to nearer ,
Using common ion effect solution

[@karnakarkadaru2317]

Sir, i have a doubt, Buffer PH to be adjusted is based on our compound Pka value or buffer pka value

[@pharmagrowthhub3083]

First, decide the required pH based on your compound and retention requirement. Once you identify the pH requirement, the next step is how to achieve the pH. For that purpose, you need to think of suitable buffer salt with the closest pKa value

[@abdulmujahid4048]

Thank you sir 🙏

[@Learnwithsimon27]

very nice

[@Jitturawat958]

Thank you sir

[@azizqureshi8476]

Plz send me pdf