RT - PCR (Real Time PCR) | Quantitative - PCR | qPCR | Basic Principle and Data Analysis
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- Опубліковано 5 вер 2024
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Real-time PCR (#RT-PCR), or quantitative PCR (#qPCR), is a highly sensitive molecular biology technique used to amplify and simultaneously quantify DNA or RNA in real-time as the amplification occurs. Unlike traditional PCR, which provides an endpoint measurement of amplified DNA after the reaction is completed, real-time PCR allows for continuous monitoring of the amplification process. This real-time monitoring is achieved through the use of fluorescent dyes or probes that emit fluorescence as DNA or RNA is replicated.
Primer Designing - 👉 • Primer Designing PCR |...
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Teaching is an Art. As student of zoology I only say thank you for such simple way of teaching
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I am first year mbbs student and couldn't understand this topic not in my college and neither on any other platform.
But you really made this easy for me.
Thanks.
Thank you Zaid
Such a quality education!!! Cheers for it and advance HAPPY TEACHERS DAY to u ❤❤
Thanks a ton
Thank you so much mam. You explained this difficult topic in a very simple way..😢❤ Huge respect and love from Pakistan.👏🙌
Mam aap bhott acha smjaati hu kaash hmaare college k time pr aapke lecture dekhe hote toh ache number score kr pata
Thank you ma'am for explaining very clearly.
Thanks for liking
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR).
Going for a interview tomorrow and spent the whole day here
All the very best for tomorrow:)
Aap nae janti but aap bht easy krdeti han har chz 😂🎀
Keep on covering my BS syllabus please🌝
Ma,am Aap 4 year,s mai bilkull b change nhi hui...
Pehley b Gorgeous thi or ab b...illlaa MashaAllah...😊❤
Thanks 😊
Thank you
It was easily understandable for exam point of view
Super madam
Thank u mam!
Really helpful
Love u mam.....
Thank you so much ❤
Your videos help me to strengthen my concepts
Please share with your friends ❤️
Really Helpful mam
Thank you
Superb mam
Thanks for watching my videos 👍🏼
Thanku
You explained the topic so well. Thanks a lot
Superb ma'am
Thank You :)
Lots of love from united states
Maam aap bahut hi achi tarah se explain karti hai
Thank you
Nice 👍 thankyou mam for besic revision
You're my favorite mam ❤️🥰
Medam so nice lecture love from ❤ Pakistan
So helpful, many thanks❤
Is chapter ko parne ki liye abhi book open kiya hai or apka video ka notification bhi agaya net on kiya to... Nice coincidence 🔥🔥🔥😅😅
I hope aapko samajh aaya hoga topic..
Well explained mam 👌🏻👌🏻
Thank you ma'am 🥰
Thank u dear
Its really very helpful mam, thank you so much dear mam 😊
Thank you
very well explained Ma'am...
Mam plzzzz RFLP and ARMS pcr ke primer designing aur practically how can we performed ARMS and RFLP pcr.
Ok.. noted
Thanx mam for your reply in ok.
Crystal clear ho gayi 😊
Thank you dear ❤️
All concepts cleared
Very helpful..tq so much
Mam please make more videos on instrumentation.
👍
Thank you mam❤
Nyc explanation mam❤❤
Thanks a lot 😊
A lot of thanks ma'am
It is really very helpful madam.thank you so much....please create also video simple PCR and sequence seqstudio thermofisher
Sure.. thanks
Nice ❤
Thank u so much ma'am
Thnx mam ❤❤
Thanks mam ,,,,
I want your special class for siqwancing ,
Please help
👌👌👌
thanku dii❤
Thank you Ma'am.
Thank you:)
Real time PCR or Reverse transcriptase PCR ????
Thank you so much ma'am
Thank you
Thanks mam
Keep watching
Thank you maam😍
Thanks Kajal 😊
❤
Thank you very much ma'am for your support 🙏
Thank you
Thank you mam ❤
Thank you Dishant
Kal internship ke report ka viva hein, Thankyou mam 🙂
how was it? :)
@@BaaYo just done with viva, i answered all of the questions correctly, let's see how many marks she has given
Actually I am preparing for CAT, just mistakenly took admission in biotechnology, but ur videos literally save me everytime a day before exam or viva
Even with 0 interest i still score 8.4 sgpa in every sem 🙂 all thanks to u
Once again Thankyou from a guy who doesn't like biotech at all 😂
Hahaha best wishes
Probe kaha bind hota hai madam
Mam reverse transcriptase pcr, inverse, nested etc inke upar v ho sake video baniyega
Sure
Maam wurtz fitting reaction free radical substitution halogenations kya hota hai maam pura video banaiye n es pr
Noted
Plz make video on immunoflouresence assay
Sure..
why mam is so beautiful❤❤
RT pcr ma hum RNA single strand use nh karte ?
Ekdum Bakwas padhaya ae topic apne ...kitna confuse kr rahai ho PCR and rtPCR me
Maam kindly explain primers ....
Becaise we have confusion
If we have unknown DNA temple how primer will join..
Ya next video will b about primers..
Thanku ma'am ❤
Mam what is the detection process of Rt-PCR..?
See the amplification curve.. if it is there then case is positive
Maam aap keval chemistry hi padhati hai ya aapke pass math ke bhi teachers hai
Lifescience.. biotechnology no maths
Mam 7:4 min pe aap ne jo bataya wo nhi samjha.
5 terminal aur 3 terminal nhi samjh raha kya hota hai. Aur quancher kya hai.
Terminal ke bare me mai dobara explain krugi.. for this you need to understand DNA structure
@@BaaYo ok mam.
Humko lab me SOP file nhi dete padhne k liye. Main quantstudi pe rtpcr lagata hoo.
Thank you.
Heloo gd evg mam....
Mam please help me i want to qualify my jrf but i Failed from my three attempt.....
Please help me mam ki mi kiasey kru or galti kya ho rhi h mhuj se....
Mam miney basic per cammand kiye h but advance tak kisey le jayu....plz guide me mam
Msg me on telegram
😢kya ct
Not so much helpful.....need more explanation about CT value
What u want to know tell me.. it is just an introductory video..