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Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@@animatedbiologywitharpan Sure, I have subscribed to you, too! The font styles are challenging for most people to read them. Because of them, I am not motivated to read included texts! I hope you don't use incoming videos! Thanx, dear Arpan!
in RFLP the GOAL is to detect specific DNA fragments after restriction enzyme digestion and gel electrophoresis. Now imagine the fragments are not transfered in the membrabe. Then hybridization is not possible. In gel hybridization cant be done.
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Thank you for your video!! But I have a question. Can I say that Southern Blotting is a technique for detecting RFLP? or RFLP itself is a different technique?
Not reliably ....point mutation might change a restriction cleavage site hence the restriction fragments in theory should be of different length in control and mutant. But now a days pcr based methods / sequencing methods are used
Pcr can amplify upto 500bp dna reliably beyond that efficiency drops badly. So in short yes it could be done but for short dna fragments but not the entire genome.
Noting can be done very accurately with this method. Its obsolete because of highthroughput sequencing. Most RFLP markers are co-dominant (both alleles in heterozygous sample will be detected) and highly locus-specific.
The extra 3 minutes was so worth it! Thank you for this!
Watch my latest video on lab grown brains ua-cam.com/video/Rhb0nYXe9Ao/v-deo.html
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Thank you so much! Visualization really helped me to understand the subject!
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VERY GOOD BRO YOU ARE AMAZING
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Thank You sooo Much such a useful explanation
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Well explained👌
Here is our playlist on "Techniques used in Biology " please scroll down through the playlist and check out whether its useful for you. ua-cam.com/play/PLKtiwIJ8Q7rpk5lT6cxL7xGOHiHjISG2C.html&si=S6qd3z74pAy2_K-4
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Best explanation!
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best explanation
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Thanks very useful
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@@animatedbiologywitharpan Ok
It was easy and helpfull
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Great Explanation!! ..... Please use the common font styles in writing, like Arial or else!
Thanks for your suggestion, we will incorporate it.
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@@animatedbiologywitharpan Sure, I have subscribed to you, too! The font styles are challenging for most people to read them. Because of them, I am not motivated to read included texts! I hope you don't use incoming videos! Thanx, dear Arpan!
Thank you so much
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Alec Jeffrey gave DNA fingerprinting, not RFLP... are they the same thing? I don't think so
Why Southern blotting is used in RFLP? What if I didn't do the southern blotting part?
in RFLP the GOAL is to detect specific DNA fragments after restriction enzyme digestion and gel electrophoresis. Now imagine the fragments are not transfered in the membrabe. Then hybridization is not possible. In gel hybridization cant be done.
Thanks
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Thank you for your video!! But I have a question. Can I say that Southern Blotting is a technique for detecting RFLP? or RFLP itself is a different technique?
In short Rflp use southern blot method. Watch the video : ua-cam.com/video/n2bVFcJP-HY/v-deo.html
@@animatedbiologywitharpan thank you so much!
Thanks 👍🏻
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@@animatedbiologywitharpan sure , it's really helped me ❤️
Thanks From Egypt
My teacher used this for our cancelled lesson
I hope it will be useful
Rflp used in SNP analysis??
If SNP alters a restriction sequence can be genotyped by 'natural PCR' after restriction digestion.
So it's mean we use SNP analysis for RFLP?
Did you know SNP analysis techniques??
Sir reply plz
may I know what international issues of RFLP if there is one?
RFLP is a labor-intensive procedure that does not have the resolving power anywhere closer that of WGS and SNP analysis.
Can we detect point mutations with this technique?
Not reliably ....point mutation might change a restriction cleavage site hence the restriction fragments in theory should be of different length in control and mutant. But now a days pcr based methods / sequencing methods are used
Sir it is possible to produce large fragments of DNA using PCR no sir From the available crime scene samples?
Pcr can amplify upto 500bp dna reliably beyond that efficiency drops badly. So in short yes it could be done but for short dna fragments but not the entire genome.
Can linkage mapping be done using this method? If so, how do we find out if a person is homozygous or heterozygous for a particular gene?
Noting can be done very accurately with this method. Its obsolete because of highthroughput sequencing. Most RFLP markers are co-dominant (both alleles in heterozygous sample will be detected) and highly locus-specific.
3 minutes but video is 6 minutes 😂
I hope it is informative enough