restriction fragment length polymorphism (RFLP) |Principle | applications | limitations

Restriction fragment length polymorphism (RFLP) is a type of polymorphism that results from variation in the DNA sequence recognized by restriction enzymes. These are bacterial enzymes used by scientists to cut DNA molecules at known locations. ... Typically, gel electrophoresis is used to visualize RFLPs.

[@tarahmd]

The extra 3 minutes was so worth it! Thank you for this!

[@animatedbiologywitharpan]

Watch my latest video on lab grown brains ua-cam.com/video/Rhb0nYXe9Ao/v-deo.html
Dont forget to like and share. Please put your opinion in the comment

[@zodafone_]

Thank you so much! Visualization really helped me to understand the subject!

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@NanduNikku]

Thank You sooo Much such a useful explanation

[@animatedbiologywitharpan]

please share my channel link with your friends and help me to reach big audiance

[@abdelhamidchihi9218]

VERY GOOD BRO YOU ARE AMAZING

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@ranjeet_sohanpal]

Best explanation!

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance.

[@WoogaChan]

My teacher used this for our cancelled lesson

[@animatedbiologywitharpan]

I hope it will be useful

[@joellopezgarcia9973]

Thank you so much

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@ARYA__2000]

It was easy and helpfull

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@rishabhkedia9304]

best explanation

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@chriswong1439]

Thank you for your video!! But I have a question. Can I say that Southern Blotting is a technique for detecting RFLP? or RFLP itself is a different technique?

[@animatedbiologywitharpan]

In short Rflp use southern blot method. Watch the video : ua-cam.com/video/n2bVFcJP-HY/v-deo.html

[@chriswong1439]

@@animatedbiologywitharpan thank you so much!

[@livinghope8039]

Great Explanation!! ..... Please use the common font styles in writing, like Arial or else!

[@animatedbiologywitharpan]

Thanks for your suggestion, we will incorporate it.

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience .

[@livinghope8039]

@@animatedbiologywitharpan Sure, I have subscribed to you, too! The font styles are challenging for most people to read them. Because of them, I am not motivated to read included texts! I hope you don't use incoming videos! Thanx, dear Arpan!

[@walaasaeed6960]

Thanks 👍🏻

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@walaasaeed6960]

@@animatedbiologywitharpan sure , it's really helped me ❤️
Thanks From Egypt

[@abdumalik_choriyev]

Thanks very useful

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance

[@abdumalik_choriyev]

@@animatedbiologywitharpan Ok

[@freedomtv1505]

Thanks

[@animatedbiologywitharpan]

Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

[@aarjvijain]

Alec Jeffrey gave DNA fingerprinting, not RFLP... are they the same thing? I don't think so

[@saifalhasan9895]

Why Southern blotting is used in RFLP? What if I didn't do the southern blotting part?

[@animatedbiologywitharpan]

in RFLP the GOAL is to detect specific DNA fragments after restriction enzyme digestion and gel electrophoresis. Now imagine the fragments are not transfered in the membrabe. Then hybridization is not possible. In gel hybridization cant be done.

[@rashmisekar3852]

Can linkage mapping be done using this method? If so, how do we find out if a person is homozygous or heterozygous for a particular gene?

[@animatedbiologywitharpan]

Noting can be done very accurately with this method. Its obsolete because of highthroughput sequencing. Most RFLP markers are co-dominant (both alleles in heterozygous sample will be detected) and highly locus-specific.

[@tamilbiology5078]

Can we detect point mutations with this technique?

[@animatedbiologywitharpan]

Not reliably ....point mutation might change a restriction cleavage site hence the restriction fragments in theory should be of different length in control and mutant. But now a days pcr based methods / sequencing methods are used

[@reshu6012]

Sir it is possible to produce large fragments of DNA using PCR no sir From the available crime scene samples?

[@animatedbiologywitharpan]

Pcr can amplify upto 500bp dna reliably beyond that efficiency drops badly. So in short yes it could be done but for short dna fragments but not the entire genome.

[@gulpypup1069]

may I know what international issues of RFLP if there is one?

[@animatedbiologywitharpan]

RFLP is a labor-intensive procedure that does not have the resolving power anywhere closer that of WGS and SNP analysis.

[@tehreemsajjad653]

Rflp used in SNP analysis??

[@animatedbiologywitharpan]

If SNP alters a restriction sequence can be genotyped by 'natural PCR' after restriction digestion.

[@tehreemsajjad653]

So it's mean we use SNP analysis for RFLP?

[@tehreemsajjad653]

Did you know SNP analysis techniques??

[@tehreemsajjad653]

Sir reply plz

[@pjshady7465]

3 minutes but video is 6 minutes 😂

[@animatedbiologywitharpan]

I hope it is informative enough