In qPCR, as for the digital PCR, the wild type strain is often considered a reference used to compared data relative to mutations. The dPCR is a method which allows the absolute quantification of a template (DNA or RNA), but it is also used to produce comparative (or relative) results, one example could be the necessity to compare the fraction of mutated sequences out of the wild type ones in a certain sample. At the following link www.gene-pi.com/tutorial/introduction-rare-mutation-detection-2-3/ you can find an example of an assay of this kind.
Of course ! This could be also one strategy. Depending on what are your expectaction you can design the assay you prefer. The naica® system offers the possibility to have multiple channels which could allow at the same time, and in the same experiment, the detection of different targets.
Yes, the PCR mix is loaded in our chips chips and then is partitioned in thousands of small droplets in which the template sequences (DNA or RNA) will be randomly distributed. For more information about our workflow, please check on our website here: www.stillatechnologies.com/naica-system-workflow/
Very nice technology and very good explanation, bravo!
This explanation was great! Thank you so much!
great video! best explanation of digital PCR I've seen so far
Thank you! If you are interested in learning more about Digital PCR please visit www.gene-pi.com/
What a great explanation, perfect for understanding this specially in Covid times.
very clear explanation！
Why do you use fluorescence markers for the wild type allel in conventional qPCR if you are only interested in the mutation allel?
In qPCR, as for the digital PCR, the wild type strain is often considered a reference used to compared data relative to mutations.
The dPCR is a method which allows the absolute quantification of a template (DNA or RNA),
but it is also used to produce comparative (or relative) results, one example could be the necessity to compare the fraction of mutated sequences out of the wild type ones in a certain sample. At the following link www.gene-pi.com/tutorial/introduction-rare-mutation-detection-2-3/ you can find an example of an assay of this kind.
Why don't we construct a primer binding with the SNP therefore only the Mutant DNA would get amplified and we can visualize it
Of course ! This could be also one strategy.
Depending on what are your expectaction you can design the assay you prefer.
The naica® system offers the possibility to have multiple channels which could allow at the same time, and in the same experiment, the detection of different targets.
Excellent video!
Very helpful and informative Thankyou
Where can i find the references?
So you separate the sample before testing .?
Yes, the PCR mix is loaded in our chips chips and then is partitioned in thousands of small droplets in which the template sequences (DNA or RNA) will be randomly distributed.
For more information about our workflow, please check on our website here: www.stillatechnologies.com/naica-system-workflow/
Nice video! Does digital PCR also need to consider the effect of non-specific amplification caused by primer?
Very nice video, i would be grateful if you could give me a reference of the Poisson law to convert the data into copies/µL/ Thank you very much !
Thanks for the great explanation!
Great tech, awesome video!
Isn't this qPCR?
amazing! thank you!
Thank you : )
I appreciate
Nice
Thompson Jose Martin Anthony Anderson Deborah