Multiplex PCR

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  • Опубліковано 6 лют 2025

КОМЕНТАРІ • 60

  • @nafisanawal1450
    @nafisanawal1450 2 роки тому +5

    Thank you so much for the explanation.It clarified my confusions.

  • @alrodezno
    @alrodezno 7 днів тому +2

    You could consider making a channel in Spanish or giving the option of placing audio in Spanish, your videos are very good

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  7 днів тому

      we are planning to be multilingual in future

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  7 днів тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @lenkajasikova5099
    @lenkajasikova5099 2 роки тому +8

    The best explanation, thank you so much!!!

  • @kiplimosimon1429
    @kiplimosimon1429 3 роки тому +1

    The video is very informative and easy to understand. Thank you

  • @phdunia9498
    @phdunia9498 10 місяців тому +1

    شكرا جزيلا لك
    وقد استخدمت الصور التي عرضتها في القاء محاضرتي شكرا لمساعدتك

  • @nawressneji7953
    @nawressneji7953 2 місяці тому

    Thanks alot for the efforts i found it verry informative

  • @emmm6030
    @emmm6030 2 місяці тому

    great explanation

  • @danilomenezes3996
    @danilomenezes3996 Рік тому +1

    Great explanaation! Thank you for your time.

  • @mathankumar5795
    @mathankumar5795 3 роки тому +2

    It is very clear explanation about multiplex, thanks

  • @itsmybio8645
    @itsmybio8645 3 роки тому +1

    Best Sir. Easily you Explained... Thanks alot

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому +1

      Please share my channel link with your friends and help me to reach big audience

    • @itsmybio8645
      @itsmybio8645 3 роки тому

      @@animatedbiologywitharpan Yes sir

  • @plingplong123
    @plingplong123 Рік тому

    Very Informative, I love it

  • @tlkruthi1066
    @tlkruthi1066 8 місяців тому +1

    Great video!
    2:47 If multiplex PCR requires that the template size does not vary too much, how would it be detected separately on the agarose gel as they would be having similar molecular weights?

  • @lisselllee6757
    @lisselllee6757 Рік тому +2

    Is it that although the amplicon size must not differ too much it must still be different enough so that distinct bands may be observed during gel electrophoresis?

  • @Fl0wright
    @Fl0wright 3 роки тому +3

    Feels like this video could be sponsored by SpeeDx - same fundamental but totally different molecular biology

  • @moxmalamakalumbi8213
    @moxmalamakalumbi8213 Рік тому +1

    Super vid. Thanks.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Рік тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @alicechoribege3736
    @alicechoribege3736 Рік тому +2

    Thank you for the clear explanation.

  • @jalilahmad7424
    @jalilahmad7424 3 роки тому +1

    Great video
    To the point
    Thnk u so much sir

  • @evelynjunaviles2582
    @evelynjunaviles2582 2 місяці тому

    Hi, thanks for this nice explanation! Do you know any online tool to create 3 set of primers for a multiplex? how can I create them so they meet all the criteria?

  • @taharyasmine5944
    @taharyasmine5944 3 роки тому +2

    Thank you ❤️

  • @khalidabdullah2561
    @khalidabdullah2561 3 роки тому +1

    thanks for the explanation

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому +1

      Please share my channel link with your friends and help me to reach big audience

  • @dr.urmibhattacharjee6919
    @dr.urmibhattacharjee6919 7 місяців тому +1

    Awesome 3 minutes . Thanks a lot.
    each of my samples contains one bacteria and one virus. Can I use multiplex pcr to quantify them in each sample and compare those ?

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  7 місяців тому +1

      @@dr.urmibhattacharjee6919 yes you can, if the pcr product size is different lets say 400bp for bacteria specific gene and 600bp for virus specific gene then its possible .

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  7 місяців тому +1

      @@dr.urmibhattacharjee6919 Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @deepikagupta4032
    @deepikagupta4032 2 роки тому +1

    You are really best

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
      I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
      You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

  • @Ms.KiruthikaV
    @Ms.KiruthikaV 3 місяці тому

    I have a doubt sir, if the 3 different primer set have slightly different annealing temperature say for eg: 53.1;53.7;53.5 which one should i choose or should it be exactly same annealing temp for all primer sets?

  • @maymunamunna6110
    @maymunamunna6110 2 роки тому +1

    thanks a lot sir.

  • @ammarahchaudhary2820
    @ammarahchaudhary2820 2 роки тому +1

    Sir plzzz upload videos of primer designing with full details.
    How can we identify rs no,
    Second for RFLP and ARMS primer
    designing videos also

  • @adbiologist
    @adbiologist Рік тому +1

    Good one just like @sciencepoint

  • @cafenoisette7299
    @cafenoisette7299 3 роки тому +1

    hello, I do not understand English very well I speak French but I found your video great however there is one thing that I would like to know in the tube we put the patient's DNA only then the primers of different viruses and we look at gel electrophoresis whether it's to amplify or not. my question is at no time we add the DNA or the viral DNA viruses can you answer me please even in English I will understand thank you

    • @Fl0wright
      @Fl0wright 3 роки тому

      Il est nécessaire d'ajouter l'ADN du virus dans un autre tube et faire le même PCR pour le positive contrôle

    • @tlkruthi1066
      @tlkruthi1066 8 місяців тому

      Viral DNA is already present in the patient's sample. No need to add it separately.

  • @sutapapal8796
    @sutapapal8796 3 роки тому +3

    So for covid we can use multiplex PCR??

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому +1

      Yes multiplexing can be done

    • @muhammadasimkhankhattak97
      @muhammadasimkhankhattak97 4 місяці тому

      Not mandatory
      It depends on what you want to detect, depends on your target's availability.
      As he told the criteria for multiplex, it must be ensured first before having targets.

  • @riyasaha7250
    @riyasaha7250 3 роки тому +2

    Sir, please make a video on allele specific PCR technique...

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому +1

      Ok sure....please share my channel link with your friends and help me to reach big audience

  • @qamarunnisa9538
    @qamarunnisa9538 3 роки тому +1

    Always best

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому +1

      Please share my channel link with your friends and help me to reach big audience

  • @hamidakhatun2139
    @hamidakhatun2139 2 роки тому +1

    Please make a video on dna fingerprinting

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому +1

      Thanks for your suggestion will make one. But i do have vodeo on southern bloting and RFLP analysis

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому +1

      Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance

    • @hamidakhatun2139
      @hamidakhatun2139 2 роки тому

      @@animatedbiologywitharpan I am following you on both

  • @jasmintea8131
    @jasmintea8131 3 роки тому +1

    Thank you ❤️