Great interview! Could you elaborate more on the energetics of glowing plants? What makes you say it’s impossible to get much brighter than we already have now? I know I’ve heard light bio has a goal of making one 10x brighter and I’m curious how they’ll pull it off. I don’t think copy number or up-regulation would work because I’ve noticed that already with this first generation product, my fireflies grown from seed grow noticeably slower than seeds from the same pod that didn’t inherit the glow. I guess I kind of answered my own question there but I figured you’d have deeper level insights given you’re in the field
@@lucash7012 plants are very low power organisms that spend most of the ATP they produce on making all the molecules the plant needs...from scratch. Because of this the available energy at any given time is much lower than an animal which eats pre-built molecules thru eating plants or other animals. This savings is exploited to allow for very energetically costly things like fast movement, heat generation, etc. We have not gotten to the theoretical maximum efficiency of any glow circuit, but it wont be very far from what we currently have. Even animals like fireflies glow brightly but only momentairly. One way to make it relatively brighter is to accumulate the glow fuel and release the enzyme that burns it to make light all at once. Any sustained glow will only be faint compared.
@@ATinyGreenCell neat, thanks for the further info! I saw in an interview between two of the licensed growers of the firefly petunia that light bio is looking into the feasibility of using genes from the mimosa pudica plant to make it respond to touch by glowing. So that may be a way to get around this limitation in a similar way to what you mentioned.
This has been such an educational interview to watch. I am an older student doing a business degree that took a leap of faith in my summer break to do horticulture as i have always enjoyed working in my garden space. It has been such an enjoyable experience due to enjoyment of the subject matter. My horticulture course introduced me to tissue culture which led me to Plants in Jars. This has all happened over the last month, and now im trying to work out how it may be beneficial as a job in industry. As an older student it is difficult to consider going back to university for further years and debt. Your content has been such a step up in translation for those of us that are fresh on the scene. the final questions were really enlightening in regard to the studies etc. I have only just started reading studies and becoming familiar with them over the last year for my bvusiness paper. Great Content! Thanks!
As a more classically trained biologist (masters degree and several pubs) I applaud both of your work. You're both perfect examples of people who do fantastic work without the arbitrary title of education. I can't wait to watch your content @atinygreencell
I'm only half way through and this interview is excellent! I have made GMOs in labs and this guy is an inspiration to anyone who wants to do this without going through all the college coursework. I especially love his plan to make the process with the sugars and not antibiotics and herbicides. This will do many things, but most importantly it will help win over some people who are anti GMO that don't want the herbicides used.
Now that I'm done watching the whole interview I have to say that I loved that he said he supports pirating the scientific papers. He is absolutely right that if tax dollars funded any portion of the research, then we should have free access to that research. The foundation of our scientific knowledge and why we finally advanced as a species/society is because of the free exchange of knowledge. The paywall that scientific literature exists behind is pushing us backwards in that regard.
I was surprised to that changing sugar metabolism could be a effective strategy for separating them... I did think herbicide resistance was good but fail to realized that pollen can cross-breed resistance gene to other plants 😅.
This is fantastic! I might have to watch this interview a few times. Really encouraging to me that some of the struggles i face with tissue culture are common for everyone, from inducting clean cultures to decoding elaborate well-funded protocols.
torturing plants is a top video for you? you dont give a crap about the suffering inflicted from these unnatural conditions towards plants. we dont even know their physiology. they evolved outdoors in wildlife environments, not these lab prisons.
@@paulnovak833 i suffered with the worst pain humanly possible in the past. the most important thing is to reduce suffering and maximize happiness. altering nature very unnaturally like this is dangerous. humans dont know what causes consciousness and suffering to exist. plus 'plants in jars' banned me from their discord only for speaking about ethical problems of altering plants. these addicts will stop at nothing.
I grow petunias indoors, while watching this FANTASTIC video the growing units in the background growing petunias caught my eye . I need to know where I can get them . And specifics on their use. THANKS SO MUCH!
They’re hydroponic test tubes, a 3d printed collar in a test tube filled with some variety of nutrients. I think the files may be available. You can also use foam.
Thank you so much for making this knowledge available to everyone. It reminds me of the GloFish I keep in my planted tanks. They are also transgenic animals that use the same fluorescing proteins mentioned in this video. The fish are flourishing in the wild in south america, usually in brackish areas where other naturally fluorescing animals also reside. GloFish benefit as the predators have learned to steer clear of fluorescing animals in these ecosystems.
It's good to know how things happen lol i realize how little I know about what I should and should not cook together. Im down the rabbit hole of learning all kinds of things because of my "spiritual" practice I follow the native way. I forage and observe nature now am getting into the complexity of soil. People are so scared and hate on GMO but we need to know what it gives!
Wait, we know what causes burl formation in trees? I was under the impression that this was still a big mystery. So are there people intentionally creating forests full of burls because they are so much more valuable than just plain wood?
Very cool discussion. I wonder how Sebastian could combine his research in the future with additional research finding the "optimal flower pattern". Maybe he could attract different species (or detract) with simple pattern changes to the petals.
@@aymanintiser8997 there is a finicky protocol called somatic hybridization where you can fuse cells of different plants together, but only when you dissolve leaves into a soup of naked cells called protoplasts. If you add Miralax (Polyethylene glycol 3350), the bulkyness of that polymer forces cells together until they fuse like soap bubbles joining. Then regenerate from single cells and pray the right bits of each parent plant combined and the resulting chromosomes are even numbered. It's VERY hard but it is doable.
You might be interested in "somatic hybridization" through protoplast fusion, which is altogether different from the kind of genetic engineering described in this video. Protoplast fusion allows for wider crosses than conventional methods of hybridization (like artificial cross-pollination), but the most stable somatic hybrids are still fairly closely related (like different species in the same genus, or closely related genera in the same family).
@@TheHKDS you can but the plant would kill it quick or die. For agro transformation to work you need to transform a bit of leaf and then nurture those few cells the agro bites into whole plants via tissue culture. Its very hard to do without tissue culture but there is a method called cut dip transformation tgat relies on a different agro called A. rhizogenes that only makes roots, and plants that can regenerate from root cuttings. Limited tech but promising! Otherwise it's absolutely required to do tissue culture.
Is it scientifically possible to genetically modify cherry blossom tree to make the blossom blue? Bcoz my favourite tree is cherry blossom tree and my favourite color is blue.
sometimes i wonder if there's a difference between like. calcium glutamate signalling and nociception in plants though if i'm honest. like functionally.
Greetings! Iris here. They perform the same ecological function in response to merophages, but for other interactions it's pretty complicated to draw functional equivalence between the two because most nociceptice organisms are heterotrophs.
Greetings! Iris here. They perform the same ecological function in response to merophages, but for other interactions it's pretty complicated to draw functional equivalence between the two because most nociceptice organisms are heterotrophs.
I treat the seeds with chlorhexidine. I bought it to sterilize my hands during the "19" epidemic. Interesting question. Is it possible to treat pieces of plants? How will chlorhexidine affect the process of root and leaf formation? Will it cause mutations?
@@saintjohnny45 I don't have access to high-quality seeds. I was looking for a reliable way to disinfect seeds. One researcher showed the results of using different simple disinfectants. Pharmacy solution of chlorhexidine was the most effective. It destroys dangerous plant diseases and does not require complex protocols.
Your telling me I can GMO my weed? How to I add ultraviolet reacting, like glow with black lights? Do i need to get a jelly fish or a reactive algea? EDIT nice so its a fungi I can use... good i was worried for the jelly FISH LOL
My question is whether or not I can solve the housing crisis and the food crisis, and climate change with a single transgenic plant species? I personally believe its possible, adding the right genes from other plants, and animals, could yield a plant that can grow into something such a size that it could live on in legend, that grows strong enough to be guided into a shape that is safe to live in, produce fruit that are edible, and be both cold tolerant, heat tolerant, and salt water tolerant. Doing so with a tree like willow would be my priority as it already has a fast growth rate, can very easily be cloned with cuttings, can be grafted together very easily as well, And it already has cold tolerance to a degree. The ultimate plan, grow a skyscraper that makes edible fruit, can root anywhere there's salt water, and produces its own light at night. And don't knock the brightness level of a firefly. That brightness only seems insignificant because of how tiny they are. At the size of a skyscraper, that brightness is enough to see by.
That's a cool idea, what about two or more trees that grow on eachother? like how a fig tree will climb up an already existing tree, the first tree could grow really tall and wide to provide the scaffold for the fig tree which grows around it, eventually fully in casing the scaffold tree which dies, leaving a hollow home within. Figs already fruit and you could graft other species into them. Or have other vines that don't kill the host plant.
I’m not trying to be rude at all, as I believe that there is much more that is possible that modern “science” would say is not, but this is a bit of a reach in my opinion. A.) what would become of the environment around these massive trees? The roots would need to be as large as the shoot, and this would require massive amounts of labor to move underground utilities to make room for these monstrous roots. Not to mention that a plant of that magnitude would completely sap all mineral and nutrient resources from the surrounding environment. 2.) you can’t live inside living trees. I don’t know if you meant that your intent is to hollow them out to create housing, but that’s not possible. No way for nutrients and water to flow up or down if you hollow out the main transport systems of the tree beneath the lignified layers. I could list a few other problems, but I think you get my point. We definitely need new ideas, and I do fully believe that you could take aspects of your idea and actually make them work, but the massive world tree-esque GMO superorganism just isn’t realistic. You could potentially genetically modify a fruit/nut bearing tree species to grow quickly enough that a plot of tightly spaced trees could potentially act as a residential structure. Obviously you’d still need some kind of building materials (sheet goods like plywood and drywall) to provide lateral strength and a weather barrier, as well as to provide uniform finished surfaces. Maybe the tree method isn’t the best choice for an actual house, it very well could be but it also might not be; however I do know that trees and woody shrubs can be used as living fencing.
Is it just me who finds it difficult to concentrate when listening to Americans speak due to the use of their favourite two filler words? All I heard is Sebastian saying "like" 132 times and "right(?)" 74 times. It's getting to a point where it's quite distracting.
scientists dont give a crap about ethics. plants are living conscious and sentient organisms too and this girl wants to put them in factory farms just like the animals. we need to promote antinatalism to reduce this harm from happening.
Thank you so much, Laur, for the interview. This was so much fun!
Great interview!
Could you elaborate more on the energetics of glowing plants? What makes you say it’s impossible to get much brighter than we already have now?
I know I’ve heard light bio has a goal of making one 10x brighter and I’m curious how they’ll pull it off. I don’t think copy number or up-regulation would work because I’ve noticed that already with this first generation product, my fireflies grown from seed grow noticeably slower than seeds from the same pod that didn’t inherit the glow. I guess I kind of answered my own question there but I figured you’d have deeper level insights given you’re in the field
@@lucash7012 plants are very low power organisms that spend most of the ATP they produce on making all the molecules the plant needs...from scratch. Because of this the available energy at any given time is much lower than an animal which eats pre-built molecules thru eating plants or other animals. This savings is exploited to allow for very energetically costly things like fast movement, heat generation, etc. We have not gotten to the theoretical maximum efficiency of any glow circuit, but it wont be very far from what we currently have. Even animals like fireflies glow brightly but only momentairly. One way to make it relatively brighter is to accumulate the glow fuel and release the enzyme that burns it to make light all at once. Any sustained glow will only be faint compared.
@@ATinyGreenCell neat, thanks for the further info!
I saw in an interview between two of the licensed growers of the firefly petunia that light bio is looking into the feasibility of using genes from the mimosa pudica plant to make it respond to touch by glowing. So that may be a way to get around this limitation in a similar way to what you mentioned.
nope thank you for confiriming my theoriez 👏🏻👏🏻👏🏻
@@lavagaming8449 ??
This has been such an educational interview to watch. I am an older student doing a business degree that took a leap of faith in my summer break to do horticulture as i have always enjoyed working in my garden space. It has been such an enjoyable experience due to enjoyment of the subject matter. My horticulture course introduced me to tissue culture which led me to Plants in Jars. This has all happened over the last month, and now im trying to work out how it may be beneficial as a job in industry. As an older student it is difficult to consider going back to university for further years and debt. Your content has been such a step up in translation for those of us that are fresh on the scene. the final questions were really enlightening in regard to the studies etc. I have only just started reading studies and becoming familiar with them over the last year for my bvusiness paper. Great Content! Thanks!
Thank you so much.
This is awesome! I hope it can be spread before all of us mysteriously disappear
Nice to see you here!❤🎉
She probably already has been hired by them 🤣
There's glow-in-the-dark flowers now? What a time to be alive...
As a more classically trained biologist (masters degree and several pubs) I applaud both of your work. You're both perfect examples of people who do fantastic work without the arbitrary title of education. I can't wait to watch your content @atinygreencell
I'm only half way through and this interview is excellent! I have made GMOs in labs and this guy is an inspiration to anyone who wants to do this without going through all the college coursework. I especially love his plan to make the process with the sugars and not antibiotics and herbicides. This will do many things, but most importantly it will help win over some people who are anti GMO that don't want the herbicides used.
Now that I'm done watching the whole interview I have to say that I loved that he said he supports pirating the scientific papers. He is absolutely right that if tax dollars funded any portion of the research, then we should have free access to that research. The foundation of our scientific knowledge and why we finally advanced as a species/society is because of the free exchange of knowledge. The paywall that scientific literature exists behind is pushing us backwards in that regard.
I was surprised to that changing sugar metabolism could be a effective strategy for separating them... I did think herbicide resistance was good but fail to realized that pollen can cross-breed resistance gene to other plants 😅.
YASS the best collab we needed 🌱
Infinitely fascinating. thank you so much for making this available
This is fantastic! I might have to watch this interview a few times. Really encouraging to me that some of the struggles i face with tissue culture are common for everyone, from inducting clean cultures to decoding elaborate well-funded protocols.
Top 10 youtube episode for m. Not just this channel but all channels. Nice job Laur
torturing plants is a top video for you? you dont give a crap about the suffering inflicted from these unnatural conditions towards plants.
we dont even know their physiology.
they evolved outdoors in wildlife environments, not these lab prisons.
@infiniteworfare-el3hi of all the things to place your concerns upon in this world you chose the suffering of plants 🤔
@@paulnovak833 i suffered with the worst pain humanly possible in the past.
the most important thing is to reduce suffering and maximize happiness.
altering nature very unnaturally like this is dangerous. humans dont know what causes consciousness and suffering to exist.
plus 'plants in jars' banned me from their discord only for speaking about ethical problems of altering plants. these addicts will stop at nothing.
@@infiniteworfare-el3hi that was you yesterday! Haha. Why watch it/participate then? Go hunt a deer or go fishing
Your videos are very informative. Thank you! Great work, keep it up 👏👏👏
I obsessed with your videos your videos is what ı want
I’ve never heard of amateur Biology before, this is so cool. Currently a Biology undergrad-can’t wait to do amazing stuff like this someday!!!
That was excellent. I have a couple degrees in this area and this was better than I could have done. Great work
I learned lots of stuff from this interview. Thank you and keep posting videos!
I grow petunias indoors, while watching this FANTASTIC video the growing units in the background growing petunias caught my eye . I need to know where I can get them . And specifics on their use. THANKS SO MUCH!
They’re hydroponic test tubes, a 3d printed collar in a test tube filled with some variety of nutrients. I think the files may be available. You can also use foam.
Thank you so much for making this knowledge available to everyone. It reminds me of the GloFish I keep in my planted tanks. They are also transgenic animals that use the same fluorescing proteins mentioned in this video. The fish are flourishing in the wild in south america, usually in brackish areas where other naturally fluorescing animals also reside. GloFish benefit as the predators have learned to steer clear of fluorescing animals in these ecosystems.
It's good to know how things happen lol i realize how little I know about what I should and should not cook together. Im down the rabbit hole of learning all kinds of things because of my "spiritual" practice I follow the native way. I forage and observe nature now am getting into the complexity of soil. People are so scared and hate on GMO but we need to know what it gives!
@@PrettyBlueSkyeEyes thats a unique lens to see GM plants through. Would love to hear your thoughts. Are you native?
Wait, we know what causes burl formation in trees? I was under the impression that this was still a big mystery. So are there people intentionally creating forests full of burls because they are so much more valuable than just plain wood?
Excellent and interesting interview !!!!!!!
Wow. That was a really impressive human being.
I was looking forward to your GMO video, finally got blessed!
Very cool discussion. I wonder how Sebastian could combine his research in the future with additional research finding the "optimal flower pattern". Maybe he could attract different species (or detract) with simple pattern changes to the petals.
I had a question, Can ya hybrid two plants at the same time while tissue culturing
@@aymanintiser8997 there is a finicky protocol called somatic hybridization where you can fuse cells of different plants together, but only when you dissolve leaves into a soup of naked cells called protoplasts. If you add Miralax (Polyethylene glycol 3350), the bulkyness of that polymer forces cells together until they fuse like soap bubbles joining. Then regenerate from single cells and pray the right bits of each parent plant combined and the resulting chromosomes are even numbered. It's VERY hard but it is doable.
You might be interested in "somatic hybridization" through protoplast fusion, which is altogether different from the kind of genetic engineering described in this video. Protoplast fusion allows for wider crosses than conventional methods of hybridization (like artificial cross-pollination), but the most stable somatic hybrids are still fairly closely related (like different species in the same genus, or closely related genera in the same family).
Sebastian is self-taught, wow !!!
Great content! thanks for sharing this... cheers🎉
Could you inject agro bacterium with your desired G inside the stem? Similar to those artificially tinted flowers at the markets
@@TheHKDS you can but the plant would kill it quick or die. For agro transformation to work you need to transform a bit of leaf and then nurture those few cells the agro bites into whole plants via tissue culture. Its very hard to do without tissue culture but there is a method called cut dip transformation tgat relies on a different agro called A. rhizogenes that only makes roots, and plants that can regenerate from root cuttings. Limited tech but promising! Otherwise it's absolutely required to do tissue culture.
@@ATinyGreenCell 🙏
I have been fascinated by the plant virus as a gene delivery for plants.
I just want flowers that glow in the dark. Like really glow.
Great job 😊✌🏻😺💕
Incredible stuff
Is it scientifically possible to genetically modify cherry blossom tree to make the blossom blue? Bcoz my favourite tree is cherry blossom tree and my favourite color is blue.
Certainly, but it depends on where you live and how much it is regulated
Amazing content.
sometimes i wonder if there's a difference between like. calcium glutamate signalling and nociception in plants though if i'm honest. like functionally.
Greetings! Iris here. They perform the same ecological function in response to merophages, but for other interactions it's pretty complicated to draw functional equivalence between the two because most nociceptice organisms are heterotrophs.
Greetings! Iris here. They perform the same ecological function in response to merophages, but for other interactions it's pretty complicated to draw functional equivalence between the two because most nociceptice organisms are heterotrophs.
Legend
"so that" 80IQ
"Such that" 120IQ
26:13 Homer Simpson's "Tomacco"...
sooo coool i wanna do this tooooooo
When are you going to sell your own plants I would love to but plants directly from you
Kinda makes me wonder what those super rich "Big" Food labs know, but will never tell us.
not much it is not easy and as productive as we all think. Yet.
I’ll come back to this later-it’s a bit too brainy for me to understand right now
Is he using CRISPR?
Cool!
Smart and beautiful, i think i love you 😂
5:30
I treat the seeds with chlorhexidine. I bought it to sterilize my hands during the "19" epidemic.
Interesting question. Is it possible to treat pieces of plants? How will chlorhexidine affect the process of root and leaf formation? Will it cause mutations?
Why do you treat seeds with chlorhexidine?
@@saintjohnny45 I don't have access to high-quality seeds. I was looking for a reliable way to disinfect seeds. One researcher showed the results of using different simple disinfectants. Pharmacy solution of chlorhexidine was the most effective. It destroys dangerous plant diseases and does not require complex protocols.
@@shadowbeast3568 I see, nice. Ive used h2o2 before but the results were... not stable
Your telling me I can GMO my weed? How to I add ultraviolet reacting, like glow with black lights? Do i need to get a jelly fish or a reactive algea? EDIT nice so its a fungi I can use... good i was worried for the jelly FISH LOL
You're*
Heyyy that's my idea!
Dr Eeeeevil
Bro is lying about the salmon sperm part😌
My question is whether or not I can solve the housing crisis and the food crisis, and climate change with a single transgenic plant species? I personally believe its possible, adding the right genes from other plants, and animals, could yield a plant that can grow into something such a size that it could live on in legend, that grows strong enough to be guided into a shape that is safe to live in, produce fruit that are edible, and be both cold tolerant, heat tolerant, and salt water tolerant. Doing so with a tree like willow would be my priority as it already has a fast growth rate, can very easily be cloned with cuttings, can be grafted together very easily as well, And it already has cold tolerance to a degree. The ultimate plan, grow a skyscraper that makes edible fruit, can root anywhere there's salt water, and produces its own light at night. And don't knock the brightness level of a firefly. That brightness only seems insignificant because of how tiny they are. At the size of a skyscraper, that brightness is enough to see by.
whatever drug you had, stop taking it.
That's a cool idea, what about two or more trees that grow on eachother? like how a fig tree will climb up an already existing tree, the first tree could grow really tall and wide to provide the scaffold for the fig tree which grows around it, eventually fully in casing the scaffold tree which dies, leaving a hollow home within. Figs already fruit and you could graft other species into them. Or have other vines that don't kill the host plant.
Imagine one mould wiping off an entire city 😆 Cool idea, but biodiversity is key.
@@nUrnxvmhTEuU good point, you could make it biodiverse but it would require more space and effort to develop.
I’m not trying to be rude at all, as I believe that there is much more that is possible that modern “science” would say is not, but this is a bit of a reach in my opinion.
A.) what would become of the environment around these massive trees? The roots would need to be as large as the shoot, and this would require massive amounts of labor to move underground utilities to make room for these monstrous roots. Not to mention that a plant of that magnitude would completely sap all mineral and nutrient resources from the surrounding environment.
2.) you can’t live inside living trees. I don’t know if you meant that your intent is to hollow them out to create housing, but that’s not possible. No way for nutrients and water to flow up or down if you hollow out the main transport systems of the tree beneath the lignified layers.
I could list a few other problems, but I think you get my point.
We definitely need new ideas, and I do fully believe that you could take aspects of your idea and actually make them work, but the massive world tree-esque GMO superorganism just isn’t realistic. You could potentially genetically modify a fruit/nut bearing tree species to grow quickly enough that a plot of tightly spaced trees could potentially act as a residential structure. Obviously you’d still need some kind of building materials (sheet goods like plywood and drywall) to provide lateral strength and a weather barrier, as well as to provide uniform finished surfaces.
Maybe the tree method isn’t the best choice for an actual house, it very well could be but it also might not be; however I do know that trees and woody shrubs can be used as living fencing.
Stop disrupting mother nature 👉 The Connections (2021) [short documentary] ❤
i cant emphasize how serious your comment is. scientists dont give a crap about ethics of what they are doing.
Lol found the cookers @@infiniteworfare-el3hi
Hmm.. Now I'm gonna think twice before i ever say "mmmm the smell of freshly cut grass"
Now it's "oh, grass blood"
Is it just me who finds it difficult to concentrate when listening to Americans speak due to the use of their favourite two filler words? All I heard is Sebastian saying "like" 132 times and "right(?)" 74 times.
It's getting to a point where it's quite distracting.
That guy does the devil's work!
This is wrong at so many levels...prove me wrong.
🙄
scientists dont give a crap about ethics. plants are living conscious and sentient organisms too and this girl wants to put them in factory farms just like the animals. we need to promote antinatalism to reduce this harm from happening.