Principle: Reducing Sugars under hot alkaline condition undergoes tautomerization into enediol form. Enediol are strong reducing agents and convertscupric ions of the Benedict'ssolution into cuprous ions. The cuprous hydroxide formed during the process of heating is converted to cuprous oxide as red precipiate.
Wow its exactly written in satyanaryana Cupric is reduced to cuprous and color is cuprous hydroxide yellow and cuprousoxide red Another good to test is osazone reaction Which involves phenyl hydrazine
Very helpful sir by the way I m not MBBS student, I am From Paramedical (MLT) field & sometimes I need to watch your lecture sir & pls sir upload a more Practical video such as it is Benedict's test Thanks sir
Does the solution turn green *to* brick red *after* cooling? I feel green ppt was due to less concentration of the reducing sugars in the solution, and brick red due to higher concentration. Right? Well explained👍
Can we use water bath to heat in all the test of carbohydrates...i think result will be proper and easy to come..if we don't use in some test the soln is coming out of the test tube hence creating difficulty to get results
you would seperate the sample, and test both separately. as far as I know - there's no way to actually test for non reducing sugars and reducing sugars within one test tube
If the Benedict’s test gives negative result then we have to slightly heat it using hydrochloric acid and then let it cool down.Then add an alkali like sodium hydrogen carbonate to break down the glycosidic bonds in the non reducing sugar and then carry out the test.
If Benedict's test shows blue at the start, dont we already know its non reducing, so why do the things after? Y is hcl used instead of water to break down sugar?
Reducing sugar in hot alkaline medium forms strong reducing agent Enediol. This reduces cupric ions to cuprous form. There is formation of Cu(OH)2 and CuO2 which gives different colors dependent on sugar concentration.
No, maltose and glucose are types of sugars- carbohydrates. Non-carbohydrate substances will definitely not have these two reducing sugars present. The solution will remain blue (CuSO4- Copper Sulphate).
Principle: Reducing Sugars under hot alkaline condition undergoes tautomerization into enediol form. Enediol are strong reducing agents and convertscupric ions of the Benedict'ssolution into cuprous ions. The cuprous hydroxide formed during the process of heating is converted to cuprous oxide as red precipiate.
This is very big sentence
What will happen if we dont heat it? Will it still change colour
Thank you so much....
@@itsmuaz749 nope
p
Clean sweeep... Covered all points..
It seems... Your student is sincere, obedient than my nouty students..
😀
lmfao teacher
Lol
Nouty 😂😂😂😂😂Rip english 😢😢Rip Such fool teachers 😢😢😢
@@MadridTV12 hi... Its me... Badri nath... I wrote sweeep also mistake.... Not observed?
Thank you for your effort. Please keep uploading videos of more biochemistry test.
Thanks
Wow its exactly written in satyanaryana
Cupric is reduced to cuprous and color is cuprous hydroxide yellow and cuprousoxide red
Another good to test is osazone reaction
Which involves phenyl hydrazine
Best video...make more video of test sir best
Nice explanation...!
You
Are
The
Best !
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Very helpfull ,plz upload more practicals of biochemistry
Thanku u for making videosa on practicals such a useful video 🙏🙏thanku so much great work
Very good.. Keep it up
Very helpful sir by the way I m not MBBS student, I am From Paramedical (MLT) field & sometimes I need to watch your lecture sir & pls sir upload a more Practical video such as it is Benedict's test Thanks sir
In which clg??
@@insightfulmotivebyzehra2309 Sanskriti University
Iam also a MLT students
@@thesleema6975 kis University se ho?
Tatty university
Sweet voice and good experiment perform
Does the solution turn green *to* brick red *after* cooling?
I feel green ppt was due to less concentration of the reducing sugars in the solution, and brick red due to higher concentration. Right?
Well explained👍
Depending upon theamount of reducing sugar in the urine the color will deffer such as green(+),yellow(++),orange(+++) and prik red(++++)
Beautiful result...... Thx sir...!
Thanks Archana ma'am 💓💓💓💓💓💓
seriouly!!!
waterbath is used for heating so the heat spreads equally
its so dangerous to shake the tube like that
it can also spill
Please upload more videos on practicals
Thank u for your wonderful vedio.nyc explanation.
Thanks dikhsha 💓💓💓
Sir if I learn nicely all ur vedios can I score well in biochem in proff?
Can we use water bath to heat in all the test of carbohydrates...i think result will be proper and easy to come..if we don't use in some test the soln is coming out of the test tube hence creating difficulty to get results
Yes
i am going to buy test tubes .
Good Informative
what was the sample used?
Urine sample
❤❤❤❤❤ good 👍
Can you use some kind of catalyst for this method , and deposit that copper on som surface ,or exsample gripping a copper mirror?
U are carrying it in alkaline medium is any catalyst further required
What is the name of this test particularly?
Useful
Awesome sir 👏 🙌 👌
Outstanding explanation
To observe the colour change clearly it should be immersed in a water bath and heated.
Why there in need to convert cuprous hydroxide into cuprousoxide form by heating??
Mam if while heating the colour doesn't change but after 15 minutes you get red ppt settle at bottom, then will it be reducing sugar
Home sugar positive test?
Or video upload kijiye sir ji practical k liye
What will the result be if you tested a tomato and if you tested a cucumber. Please help.
Nice video 😊
Name of the sample added?
Milk as a sample le to positive test hoga?
Good
Sir if we have to recognize non redusing sugar and reduisng sugar which are in one solution ,how can we identify them separately?
you would seperate the sample, and test both separately. as far as I know - there's no way to actually test for non reducing sugars and reducing sugars within one test tube
If the Benedict’s test gives negative result then we have to slightly heat it using hydrochloric acid and then let it cool down.Then add an alkali like sodium hydrogen carbonate to break down the glycosidic bonds in the non reducing sugar and then carry out the test.
Hi, may I know the benedict solution will reacts faster in less or more concentrated glucose solution?
More
In alkaline medium
If Benedict's test shows blue at the start, dont we already know its non reducing, so why do the things after? Y is hcl used instead of water to break down sugar?
i think it's to determine the concentration
Thank you so much sir😊
Why should we add only 8 drops of urine because it is a semi quantitative the thing is what happens if we add more drops of urine or less ?
If u add more .the non- carbohydrate which have a reducing property gives false positive for Benedict test .
It’s a pipette not a droper
Pasa marra
Sugar can be tested in a diabetes patient by। Benedict's test or barford test
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Sample means I mean kesa sample
what we can take in it
Please tell the composition of benedicts reagent
It's given in video when Benedict agent was mentioned
Provide other exp too
Keep it up
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Thanks a lot
Do you do online teaching sister?
Thank you !
Need Benedict test for making assigment
Why heated alkaline is used
Benedict's reagent react with reducing sugar color get yellow,.why?
give the scientific reason easy to understand...
Reducing sugar in hot alkaline medium forms strong reducing agent Enediol. This reduces cupric ions to cuprous form. There is formation of Cu(OH)2 and CuO2 which gives different colors dependent on sugar concentration.
when auto analyzer was not there how pathologists measured blood sugar?
By manual methods on colorimeter
Sample Kya cheze ha?
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How to make sample
What are these gm%? Is these amounts for glucose?
gm/dl
Gm glucose present in 100ml solution?
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Thanks mam
Does a non-carbohydrate substance be positive in Benedict’s test?
Nope
No, maltose and glucose are types of sugars- carbohydrates. Non-carbohydrate substances will definitely not have these two reducing sugars present. The solution will remain blue (CuSO4- Copper Sulphate).
Yes.Non_ carbohydrate like uric acid,ascorbic acid ,glutathione give false positive for Benedict test
What is the sample mixed of
Urine
Why don't u sue thermostatic controlled water bath
What is sample
Rajesh koothripali
Oh my I remember him from Big Bang theory
Same
Me too
This is sad
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What is gm%?
Gm/100ml
Please next time use a water bath
Any pdf
اختبار عملي
It is not right method.
Freshy 😂😂😂
DILI MAKA CHECK OUT. IF DI MAKA TAN.AWS VIDEO HAHHAAH
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