Staining of nucleic acid by acetocarmine 12th class bio.practical.no 13

Staining of nucleic acid by acetocarmine
Procedure
(i) Peel off epidermis from the fleshy leaf of onion and put it on a slide.
Add a few drops of water over it to avoid desication.
(ii) Cut out a small piece (about 0.5 cm size) of the epidermal peel and
discard the remaining portion.
(iii) Wipe out the water with a filter paper.
(iv) Put 2 drops of acetocarmine on the epidermal peel and heat gently on
a spirit lamp.
(v) Apply a coverslip over the peel avoiding air bubbles and wrinkles of
the material.
(vi) Wipe out the excess stain with help of blotting paper.
(vii) Examine the material under low magnification of a microscope.
Observation
Record your observations with regard to shape of cell, the number of nuclei
and their position in the cell. Draw a diagram based on your preparation
and label its parts.
Discussion
Nuclei in cells are extremely rich in nucleic acid which exist in a conjugated
form with protein to form nucleoproteinous structures, called chromatin
fibres/chromosome
Questions
1. What are the building blocks of the nucleic acid?
2. What is DNA and how is it different from RNA?
3. Name different nitrogenous bases present in the nucleic acid
12th bio all practical
1- • To study Pollen tube g...
2 - • To calculate percentag...
3 - • To study of reproducti...
4 - • To study the t.s of ma...
5 - • Study of stages of mei...
6 - • Identify Common Diseas...
7 - • identify various stage...
8 - • Mitosis in onion root ...
9 - • Determine the water ho...
10 - • Determine the pH of di...
All main practical here
Subscribe karna na bhule
Aao dhamal karte he lab me ..

[@nursingeducation-bj7dn]

My dear sir shandar practical lecture,👍👍

[@ashwinchauhan1061]

Thank you for this, and wish me luck as i have my bio practical exam tomorrow 🙌🏻😂

[@neelnayak2402]

Sir from tomorrow my practical exams are starting and bcz of your videos I m 100% sure that I will get 45+ marks out of 50

[@dashrathsingh4385]

Sir mere school mai lab nhi h . Apki help se acha score kr paungi . Thank you . You are superb sir. Thank you so much 😊

[@rajpatel7819]

Mene sabhi practical issi channel se sikhe hai 🙂🙂🙂🙂🙂 thank you sir 🤗🤗🤗🤗🤗

[@nimishaparasiya21]

Thank you so much sir.... Mera kal board ka practical hai.... Thank you

[@agguayabo7073]

i have a question, where i can read the mechanism of the reaction between aceto carmin and acid nuclei?

[@ramkripalchaurasiya460]

Thank you sir today is my practical exam

[@mohitrohilla3048]

Kya baat hai sir ji

[@poonamrohilla2954]

Supperb sir👍👍

[@preethambv5887]

Shape of the cell: long rectangular brick shaped
Number of nucleis per cell : one

[@sevenrohilla07]

Super sir ji 👌👌

[@hemanshpatelhemanshpatel9231]

Thank your sir. ❤️🙏

[@vinayrohilla3789]

dear sir yr practical really very superb

[@Weakest_Gamestation]

A very big thank you to u SIR
Amazing and understandable explanation

[@LetMeExplainnowbharatpanwar]

All the best

[@madihakousar8649]

Nice vedio sir

[@mohitrohilla3048]

Very nice

[@bkgalaxian8611]

Very helpfull

[@hemantpatel6221]

Very nice sir

[@zaraansari2169]

Thank u sooooo much...🥺🥺

[@bhavyavakani4107]

sir in our school we were able to identify the nucleus within the range of 10x power of the lens, could you explain why so?

[@arianaagrande342]

Healthy nucleus 😅😂

[@neetdreamer354]

Nice

[@spiritwarrior7461]

Acha hai

[@Princechaubey2008]

Perfect

[@muktapatil6217]

Thanks sir

[@mohitrohilla3048]

Gjb sir ji

[@yashyadav3402]

Sir me gseb board se hu ...
Sir ye practical perform krne ke baad koi writing work krna padega supplementary me?

[@RajveerKatara07]

Yes

[@yashyadav3402]

@@RajveerKatara07 pucha hi nhi ..Bach gya 🤣

[@RajveerKatara07]

@@yashyadav3402 ok

[@LetMeExplainnowbharatpanwar]

Yes

[@yashyadav3402]

@@LetMeExplainnowbharatpanwar sir as you said ...phod diya 🔥🔥🔥

[@HarshParmarviolin]

thank you sir
kaal practicle ni exam che.

[@LetMeExplainnowbharatpanwar]

All the best..jao dhamal karke aao

[@patelpratham924]

Same meri bhi hai

[@nimishaparasiya21]

Hii Harsh.. Kale mari biology ni practical exam che.

[@HarshParmarviolin]

@@nimishaparasiya21 All the best

[@murtazabekhushi3043]

hattt ..kya chudaap tha ye...

[@thakorshakti26]

To ghar ja ke kar na

[@shreenivasaseenu3609]

Worst

[@vijayabasavaraj7537]

Video is not in clarity more over ur explanation is very poor..

[@hmanjula4445]

Thanks sir

[@LetMeExplainnowbharatpanwar]

So nice of you