Mitosis in onion root tip 12th class biology experiment
Вставка
- Опубліковано 1 лют 2020
- Procedure
Growing of root tips
Select a few medium-sized onion bulbs. Carefully remove the dry roots
present. Grow root tips by placing the bulbs on glass tubes (of about 3-4
cm. diameter) filled with water. Care should be taken so that the stem portion
of the bulb (basal part) just touches the water. A few drops of water may be
added periodically to compensate evaporation losses. New roots may take
3-6 days to grow. Cut 2-3 cm long freshly grown roots and transfer them to
freshly prepared fixative, i.e., aceto-alcohol (1:3:: glacial acetic acid : ethanol).
Keep the root tips in the fixative for 24 hours and then transfer them to 70%
ethanol (for preservation and use in future). Onion root-tip cells have a cell
cycle of approximately 24-hour duration, i.e., they divide once in 24 hours,
and this division usually takes place about two hours after sunrise. Therefore,
roots grown on water should be cut only at that time to score maximum
number of dividing cells.
Preparation of slide
Take one or two preserved roots, wash them in water on a clean and grease free slide. Place one drop of N/10 HCl on the root tip followed by 2-3 drops
of aceto-carmine or aceto-orcein stain on it. Leave the slide for 5-10 minutes
on a hot plate (or warm it slightly on spirit lamp). Care should be taken that
the stain is not dried up. Carefully blot the excess stain using blotting paper.
Now cut the comparatively more stained (2-3 mm) tip portion of the root
and retain it on the slide and discard the remaining portion. After
(10-20 seconds) put one or two drops of water and blot them carefully using
blotting paper. Again put a drop of water on the root tip and mount a cover
slip on it avoiding air bubbles. Place the slide in between the folds of blotting
paper using the fingers in such a way that the cover slip mounted on the
slide is properly held. Now slowly tap the cover slip using the blunt end of a
pencil so that the meristematic tissue of the root tip below the cover slip is
properly squashed and spread as a thin layer of cells. Carefully seal the
margins of the cover slip using molten paraffin wax or nail polish. This
preparation of onion root tips cells is now ready for the study of mitosis.
Study of slide
Place the slide on the stage of a good quality compound microscope. First
observe it under the lower magnification (10 X objective) to search for the
area having a few dividing cells. Examine the dividing cells under higher
magnification of the microscope to observe the detailed features of mitosis.
Questions
1. Suggest names of a few tissues, which are suitable for the study of mitosis.
2. Why is mitosis also known as equational division?
3. What shape would a metacentric and a sub-metacentric chromosome exhibit
during the anaphase stage?
4. How does cytokynesis differ in plant and animal cells?
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All the best.. Try to preform. Better and good
@@LetMeExplainnowbharatpanwar sir THANK U VERY MUCH tamara lidhe mare 40+ avse practicals ma.. tamara video joine gayo to and WATER HOLDING CAPACITY wado exp pan avyo to aa me tamara lidhe sarkhi ritna karyo.. thank u SIR
@@DrJ-cq8en I had ph of sample and mitosis in onion root tip and reproductive structure of hibiscus
@@ent_rocherer2080 hope you had done your best bro
How did it go?
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bhai bahut clearly explain kiya aapne. Thanks a lot.
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People are commenting using English and the video is another language though it is good content other people who don't understand for them is a language barrier
Sir i am having my practical xam tomorrow......I have seen this video atleast 10 times today......
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Sandar sir
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All the best
Thank you so much sir
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I'm unable to grow onion root...😢
Thanq so much sir . I have understood clearly. Thanks for making this video. Tmro I have to attend my final practical exam. And I'm from AP
😍😍
Thank you for video. Why you didn't squash root tip?
Thank you sir😎
Tq for this video sir 🙏
Sir in school exam we have not time to wait 24 hr
So how to prepare slides in that condition
(Can we use HCl and direct stain and heat and prepare our slides) ??????????
Sir, maine bhi ye slide aese he banai hai but stages identify ni ho pati in compound microscope. Please help ihave practical
Thank you bro
Thankyou so much sir 🙏
Thanks sir
Thank you sir 🥺❤️
Nice
Sir can we use safranin in place of acetocarmine
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Most welcome
Thank u sir
sir bhut bhut dhnyawad
Everyone incoming for practical 💀💀
All the best dear
@@LetMeExplainnowbharatpanwar thanks sir
Can we take dry rooted onion instead of freshly grown rooted onion???
Fresh
Tomorrow is my bio practical n its totally depends on your vdos sir
Don't worry.. rock karke . Aaoge
Sir why my ethanol is dark pink
Tqq sir 🙏🏻
Love umta
Thanks
Last me aapne bhi kisi aur ki slides share ki hai
Tomorrow is my board practical😂
Do you make chemistry practicals?
Yes.. Available as soon as possible
@@LetMeExplainnowbharatpanwar sir will you make physics practicals??
Sir plssss phy and chem also!
For practical
Mine board practical exam is today 12 pm and I am watching this video
How was your exam
@@LetMeExplainnowbharatpanwar good 👍
BHAIYA exam me 24 hrs kaise Chor de 😂
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Becoz our practical exam on 21 march
Sir, tomorrow my board practical exam of bio can you give some information about practical.....
Any questions. Wish u very all the best
@@LetMeExplainnowbharatpanwar thank you so much sir
Sir kya isme 2021 ke sare practical hai ??????
Yes
Ok guru ji tq guru ji
O bhai, Pls comment dekh lena yaar.
Mera scl ne ye video suggest kiya hai practicals ke liye. Bhai, number lage to bolna, lekin pls observations de do. Mera fati pari hui hai. Kuch samajh nehi aa raha . Pls yaar, comment dekh lena aur reply karna.
--- *Username*
A tensed , morally fucked up mediocre class 12 PCMB student
7990975404 ask me question on this no.😊
@@LetMeExplainnowbharatpanwar Thanks brother.. 🙏🏻🙏🏻
3:1 solution preparation kaise karu thora bola to accha hoti ha???
Exam mein permanent slide 10x mein rahe ge ya 45x mein
10x per
Sir is this for gseb boards??
Yes
Yes
vaiya exam me kaise hum 24 hr rakhe?
please batana
All the preparations have to be done before 1 day of the exam.
vaiya kya hum student ko kar ke le jana pare ga ? naki school sab prepare karke rakhe ga ?
@@chaitikarmakar5091 school wale karke denge solutions prepare
okk vaiya thanks 😊
Sir why we use only onion root tip?
Because root tip is the region that has active meristematic activity..
Because growing fast easy to observe
In order to observe mitosis: we use onion root tips
And in order to observe meiosis:
We use onion anthers...
See I add something new to your knowledge..😊
Comments baad me padhna ... Pehle video dekh lo... ☺️☺️😊
Watching this before 2 hrs of practical... Only legends can do this 🥀
Q. What is the requirement of squashing the root tip?
Q. Any other substitute for acetocarmine ?
Q. What is the reason behind heating the root tip with n/10 hcl and acetorcarmine ?
Treatment with acid and heat is used to break up the cellulose cell wall allowing stain to permeate the tissue and makes it easier to squash the tissue on a microscope slide. Aceto-orcein stain turns chromosomes a purple-red colour.
Carbol fuchsin , toluidine blue, feulgen are the alternatives. ... Feulgen is the best alternative which is a specific nuclear stain unlike Acetocarmine.
24 hour k lye rakhenge toh fir practical he khtm
@ITz Himanshu ( fixation school wale karke denge 😂 )
Yes
Fixation toh duur ki bàaat hai hma toh matar(peas) bhi khud hi laane pade 😂 practical k liye
😂 hahahaahha
Photos copied hai
Konsa...no sab orignal he dear..ager apne kai or dekha he to..vo logo ne mera copy kiya hoga.
Ye origanl Contain he. Video dekh lo
@@LetMeExplainnowbharatpanwar oh thanks bro
Sir apne acche tarike se nhi samjhaya video mai 😑😑