Allele specific PCR | Amplification refractory mutation system| PCR amplification of specific allele
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- Опубліковано 28 лип 2021
- Allele specific PCR (ASP)
Amplification refractory mutation system (ARMS PCR)
PCR amplification of specific alleles (PASA)
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Ma'am your video on Developmental biology helped a lot for my exams!! Thanks a lot! Continue making the awesome videos! Support 100 🍀
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Deeply thanks 🙏🏻❤..
I understand this lectures very will..
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Please I have 3 questions: 1- is the reverse primer only one (that is two forward + one reverse = 3 primers) ?
2-are the allele specific PCR and tetra-ARMS PCR the same techniques ?
3- in some papers I read; sense primers and antisense primers, which of them is forward and reverse ?
I understand this perfectly. Thank you
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Wonderfully explained! Thank you!!!!
Thank u so much
Thank you so so much. It helps me a lot. ❤️🙏
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Very well explained ma'am. Thank you...
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Many many thnks mam but plz provide rt pcr
Simple and nice
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you are a great
incredible the way you explain such ugly topics
I wished you explain other types of PCR and PCR otimization
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@@VoiceofMalinki
I would be grateful if there is any video for you about touchdown PCR doctor?
It would be of great help 💝
Ma'am I gone through this video and it's very much good but I have a question
As you mentioned in the video there is a point g mutation, how actually we find that g mutation as I feel like in the example we know the mutation point we just developing primers to provide that but how actually we find that the mutation occurred in g
Hi Rajiv, if mutation occurs in G, your forward primer will properly anneal with this and amplify the sequence, if amplification occurs you can get band in gel electrophoresis that you will run after PCR. If mutation doesn't occur, primer will not bind accurately because of the non-complementarity, and amplification will not take place, you wont get any band in gel .
Ma'am please reply on this
@@Rajiv29_07Taushif Did you get the answer? I have already explained here.
@@VoiceofMalinki I am getting now ma'am
Thank you
Thank you so much ....
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@@VoiceofMalinki why we only use one reverse primer ?
@@purepurple956 Here, we have made the forward primer just complementary to point mutation site, a primer is may be 18-20 nt long. But the reverse primer is far away from that site. It is not complementary to mutant site. That's why the reverse primer remains same in all cases. If you make reverse primer, complementary to point mutation site, your PCR product would be too small, may be 18-20 nt long, that is not possible to analyze in agarose gel.
@@VoiceofMalinki what about if the mutation occur near to the reverse primer ?? As we know the mutation may occur in any part of gene.
@@purepurple956 Mutation is occurring in both the strands. But, we are choosing one primer (forward or reverse) that is complementary to mutant site, but other primer is not complementary to that mutant part. If we choose, both primers complementary to mutant site, the product would be too small, as it is a point mutation.