Primer Designing | Allele (SNP) Specific Primers Using BatchPrimer3 || ARMS PCR || Tutorial 9

Allele specific primers are used in ARMS PCR for different objectives to be achieved. One of the objectives is to identify the heterozygosity/homozygosity or carrier identification. In this video you will learn about ARMS PCR, designing the ARMS PCR primers (Allele Specific), Allele Flanking Primers and much more through animations. How to identify Wild Type or Mutant Allele etc. are the questions to be solved in this video.
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#allelespecificprimers #primerdesigning #sciartfun #armspcr #pcr #primers #alleleflanking #allelespecific

[@MohammadMahmoud-ji1jc]

Thank you

[@RoshanAliAcademy]

🤗

[@hilalahmad741]

Very informative video

[@RoshanAliAcademy]

Buht merbani Hilal

[@yahyajaber3401]

please, if primers in PCR-AS technique have same product size how you can discriminate between them

[@RoshanAliAcademy]

There are two simple solutions. First solution is that you use the either a two primer method or three primer method where there is no amplicons of same length. If you still want to use four primer method, then you will have to first manually check if they give same product size or different. If same, then don't use those primers, change your primers.

[@khoatong8717]

Hi the website is not working again, can you please check it?
Thank you

[@RoshanAliAcademy]

You are right the server has been down since long. I have been trying, but it's not working. It was the most useful and easiest tool I ever found. But I don't know know why they abondoned this tool for good

[@user-fi9tt6xo1g]

How l can primers desing in tetra-arm pcr

[@RoshanAliAcademy]

The video is coming

[@dr.mohanadbiotech5203]

The site of Primer Design Server 1 (Albany) is not working. why?

[@RoshanAliAcademy]

It's working. I just checked it. Please check again. Use a different browser.

[@RoshanAliAcademy]

It's only working in Google chrome with me not in Mozilla. Also try to update browser.

[@dr.mohanadbiotech5203]

@@RoshanAliAcademy I have updated google chrome, but the site still not work I don't know why

[@dr.mohanadbiotech5203]

@@RoshanAliAcademy now its ok .. the site is working .. thank you .. please can make video lecture about primer design for RAPD technique?

[@RoshanAliAcademy]

@@dr.mohanadbiotech5203 Ok. You are welcome.

[@MohammadMahmoud-ji1jc]

please answer question on email

[@RoshanAliAcademy]

Which question❓

[@MohammadMahmoud-ji1jc]

@@RoshanAliAcademy Please answer the last request on email, حسبي الله

[@RoshanAliAcademy]

@@MohammadMahmoud-ji1jc Kindly watch the new video uploaded. Your question is answered in that video.