Allele-specific oligonucleotide | Detection of Mutation | Basic Science Series

[@mahdiomidvar564]

thanks a lot

[@BasicScienceSeries]

Thank you for your valuable comment! Much appreciated you feedback

[@mahdiomidvar564]

@@BasicScienceSeries it is me who should Thank you for your efforts and savings hours of our studying time.

[@BasicScienceSeries]

@@mahdiomidvar564 Thats very kind of you !

[@mahdiomidvar564]

@@BasicScienceSeries God bless you...

[@martinabesoesonoasangono2149]

But how does the technique know where exactly is the mutation ?

[@BasicScienceSeries]

In this case we screen for mutilation, for identifying a location of new mutation we need to first find its by using other methods like NGS

[@DrNirmalyaGhoshal]

Very informative

[@BasicScienceSeries]

Thank you. I am glad you liked the content.

[@angelemmanuel4197]

What is the diference between ASO and Taqman probes for genotyping???

[@BasicScienceSeries]

Differ in design and mechanisms. Good question

[@user-yo7od8yo7v]

Why is this single base mutation designed in the middle of the probe instead of both ends?

[@BasicScienceSeries]

This helps the probe to attach better with the target site. Thank you so much for your comment, I hope it was helpful. Good question

[@user-yo7od8yo7v]

@@BasicScienceSeries Thank you so much!