Rhizomorphic Transfer | Agar to Spawn Bag
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- Опубліковано 4 вер 2024
- In this video I show you how to transfer rhizomorphic mycelium and transfer agar to a spawn bag.
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boomershroomer....
I just done 8 different varieties, agar transfers are the only way to isolate the dominant genetics you want. Thanks for sharing, this will help a lot of beginners!
Thank you! I’m still trying to learn the best strings to transfer.
I just started Agar work myself. And the speed in which the spawn bag has colonized is absolutely incredible. I don't think I could go back to just innoculating with mss. It's been 2 weeks since transferring Agar to spawn bag and almost fully colonized.
You gotta try liquid culture. You can go from injecting the bag to putting mushrooms in your mouth in 30 days @@JT-ck1sg
I feel like I took the red pill today. How deep down the shroom rabbit hole will we go??!
Hahaha
😂
All the way! Once you start down the rabbit hole it’s only a matter of time before you’re spawning
@@justjj5802 Lmao!!! I'm currently spawning after 3 solid months of exploring the rabbit hole
Further
Rhizo or tomentose, it makes no difference as far as potency all cubes have both. The difference is rhizo grows faster because it is looking for nutrients. Tomentose is happy to stay put because it is finding lots of nutrients. One can become the other if conditions are right. Don't get caught up in the importance of rhizo, it grows faster, that's it. You can prove this yourself in experiments
Thank you for explaining😊
Thank You
thank you!!!!!! I've been wondering on the sealing of the bag and a lot of people skip over that step so thank you!
Glad it was helpful! Thank you!
Lol im tellin you; a piece of paper folded over and a hair straightener. Bam.
@@nicinightbird thank you!!!
a very informative video on agar preparation.. now i know i should choose 'little ropes' over 'fluffy clouds' :)
Yep! I just transferred a bunch today!
So happy you did this episode; was wondering what to do when agar was ready. Ty 🙂🍄
Sweet! Just transfer to grain!
How did it go? I have 6 full agar plates to transfer to grain bags
Thank you for both the how to make agar selections and your time laps work is so great to watch! Thank you for your hard work. I know it isn’t easy
My pleasure! Thank you! ❤️🍄❤️
I love the way you keep everything simple. Your videos have helped me alot. Thank you
Best video of all the ones i could find on UA-cam covering this topic! Great stuff 👍
Wow, thanks!
We love your channel!
Keep up the great work!
Steve, from Dallas
Thanks so much! 😊😊😊
this was simple & informative !
you make it look simple :)
Thank you!
@@BoomerShroomer fyi: i share your videos more than I share mine LOL
😊😊
Love the time lapse
Ryzomorohic and tomentos are from the same sample but the ryzomorohic is the more solid branching?
Don't want to look dumb here but it's the only way to learn.
You are an excellent teacher by the way! Lol
I e learned quite a bit from your vids.
I’d always be interested in seeing a Time-lapse for anything that you do. I don’t see any sports syringes on here. Do you always use agar?
More creators should use time laps. Nice touch !
I didn’t disinfect my hand and I put it threw a block and it did better than it was doing before it had no contamination after either
Love that time lapse!
Thank you!
You are a natural at sectional !Congrats on your blow up tote
Thank you so much!!
So do you keep the original dish you transferred from and let it keep going?
I've learned so much from you and Willy. Thx so much!
Fyi, tomintose usually happens when it has more nutrient rich food. When it doesn't, it streches further to look for it, and that is ribomorphic.
What about when mycelium all turns into overlay immediately?
Perfect duration
Thanks.
Rhizo has no bearing on fruits, yield, potency etc. Just looks cool
Great video: thanks. What is the weight of your grain bag? Just trying to get a feel of the ratio of agar to grain. Any advice would be great! Thanks
What is in the spray bottle your using
How do you tell the difference between the desired mycelium and any number of other moulds and things that may grow?
Mold and bacteria look different in color and consistency, and they all grow faster than mycelium too so if you have a contam that still looks like it could be mycelium, it will still grow much quicker.
Think I'm going to try to do this whole process
Great video, thank you. I wanted to know the media you used. Is it pda or mea ?
Omg ash. Killing it. ThNks my girl
Thank you!
how long does the mycelium in the agar keep?
Love all of your videos! Thank you!
is agar to agar transfer better than spore to agar? I'm just curious which one is better.
Can i put agar into an all in one bag?
Is this step necessary to cut out a small piece and transfer to a new agar dish at 1:05?
What will happen if I just cut out everything into small pieces with a diversified genetics and large quantity, and then transfer to a spawn bag?
Why do spore prints to regrow a mushroom versus a culture of a mushroom to regrow a mushroom
They both grow.
what is the other type of mycelium for?
Where do you store the plates?
Hi. Sorry for the stupid question, but where do you buy these bags??? I can't find them anywhere with a square in the middle for poking spores out of a syringe.🤔😯
Amazon.. or any of the mushroom selling internet sites.
Which type of spray are you using, H2O2 or alcohol, etc?
That’s a massive knife 🔪
lol yep. I hadn’t bought blades yet.
Great video, you just had to shake it more! ... I mean, the bag really good skill and technique
Thanks 👍
Thanks 👍
Hello are them from cloning mushrooms?
What kind of Vacuum Sealer are you using and where can I get one?
Awesome thank you!
You’re welcome! 🙂
Heh.. 4:20. Good vids. I subbed
Thanks!
isnt mean... that the mycelium grow weaker?.. pda to pda..thanx
What about a bunch of water droplets inside on top
Are they on top of the mycelium growth or condensation on the top of the petri dish?
@@BoomerShroomer thanks for reply! Its condensating on ceiling of petri dish but seems to be slowing in growth? Used 3 micron micropore tape was all pearly and clean bright white for a while and looks like slowely spreading through agar now looks like the mycellium might be urinating and possibly using energy to grow into agar as its dulled a bit in color and lost its beautiful white fur somewhat? i tipped it to try and drain to no avail so a tiny bit of puddling on agar happened but that seemed to condensate back to the ceiling of jar by next day idk if its cause i used cheap agar maybe why its slow to spread out been like a month lol in constant light seems to be alive slowely spreading though no green at all or grey mold hair the drops seem to stay on ceiling of petri dish so idk lol its surely not a thick sample like yours i might have to try to go to another petri with a sample of what little mycelium i got from it i guess
Thanks vor the video❤
For the spore prints in aluminum foil, do you refrigerate those or keep them at room temperature?
Ive been keeping them at room temp. They are in a drawer in my office.
I have a scrapbook lol
What if I don’t have anything to heat snd seal bag? Do I just fold and duck tape?
Yep, fold it over and tape it up.
You can use a hair straightener if you cover the bag with a piece of computer paper first. (Dont put the straightener directly on the plastic). Theres a guy on youtube who did it if you search it lmfao
Kitchen knife and no gloves?
as long as you sterilize everything, you should be good-nothing wrong with it, especially when she's only using the knife to manipulate the agar.
But once you transfer I start to see black spots on my agar from contamination. Is there a safer way?
Thank you. Great video. Besides, any reason why you use a knife?
Because I didn’t have a blade. That's all I had at the time.
@@BoomerShroomer i love your channel and your kindness, thanks for sharing all that. Id subscribe twice if I could
@@drdzdd aww thanks!
If my agar plates are filled 3 weeks in and don’t need yet , should I put in fridge?
I put them in the basement. You could put them in the fridge but then you have to let them come back to room temp before you use them. Just a cool place will be fine.
Does agar mycelium need to be transferred to grain or can I transfer it the all in one growing kit: coco quir and manure and all that good stuff?
Grain first then bulk.
How did you sterilize the knife you used to cut into the agar? _
I used 70% isopropyl. But flame sterilization is best.
Forbidden 8 slice pizza
Hi! Does one have to add all 8 wedges to one bag or can one put in two or three wedges in a bag? Putting the rest in multiple bags. Thanks!
Yep you could put a couple in one and a couple in another. Just will take them longer to colonize.
Which size of petri dishes do u recommend?
100 mm/15 mm
good shit
Thank you!
Flow hood? Gloves?
Hi im a student from Media Art , so I have no idea on Biology background. I just love the shape texture and I would like to do an uni project to grow some mold/ fungi and took some closeup photo from it. I wonder how can I find those sample and put on the petri dish at the first place? (From start) Or is there any searching “keyword” how to get this sample from a first place? Can I get it from supermarket? Thank you so much in advance
I would recommend buying a few plates from tip of the cap and just leaving them out in the open. I promise mold will find them and grow.
Do you need a vacuum sealer?
No, you need a bag sealer or you can tape the top too.
Ashley is the kinda girl that when she turned 18 the parents moved out… alpha
LOL! Thank you!
How many agar slices would you recommend for a 1 pound spawn bag inoculation?
Maybe 2 triangles.
When do you pop open the GE patch? or is the latent air inside the bag enough after inoculating?
I don't under you question? What is the GE Patch? the filter on the bag?
@@BoomerShroomer I kind of over complicated the question, but yes.. When do you take the seal off of the patch for gas exchange.. Right after inoculation, or do you let the mycelium get established.
@@M311Y Leave the filter on. It allows for gas exchange. I never take it off.
Do you know how long these petri dishes would be shelf stable afterwards?
A few months depending on environment.
@@BoomerShroomer I imagine a cool dry place is what I'd be aiming for? Would it do okay in a refrigerator?
@@deityofgreed I wouldn’t put them in your fridge. They will stop growing.
I see that you place the agar "mycelium side" facing down on the new plate. Is there a specific reason or does it grow better? My agar seems to have uneven growth, but no contamination. Could the placement differ the results vs placing it mycelium side up
It grows faster because the mycelium covered side has direct contact with the new agar and can directly grow on that.
How long will the rhyzomorphic mycelium keep?
It will continue to grow and grow.. usually a few months and then they start to bruise. I would recommend using them as soon as they are fully colonized.
do you store your agar in an incubator to keep them at a certain temp?
I just keep them in a tub in my mushroom room. Its about 70 F in there
@@BoomerShroomer thanks!
What temprature do you leave the petri dishes at after transfer?
Usually around 70-75F.
Do you know of anyone selling made up agar cups?
Yes, there are many people online selling them! I am not sure the best one.
@@BoomerShroomer Awesome! I don’t have some of the supplies or the time at the moment. I am interested in making my own just not now. Most expensive product that I don’t have is the pressure cooker. What are a couple of sites that you think would be ok to buy from? Thank you!
Homesciencetools.com reliable?
@@timsadowski7900 www.shroomsupply.com/ is very reliable
Aliexpress has a good selection of cheap very cheap bags
Cool thank you.
Are you using a kitchen knife? lol
why do you use such a large knife? lol
That is all I had at the time.
Yikes!
Best not to let your hands or anything touch the insides of the spawn bag
kitchen knife, no gloves, wtf
How long are the agar
plates good for?
2 month or so.