I don't do anything with mycology but randomly got your broke boi tek video recommended and binged this channel since. I have OCD and mild aspergers and get stressed to hell when following instructions/ "how to's" in general but something about this is just so calming on a visceral level. Thx man.
Heya. Wondered if you’d like a tip (which to be fair you might already know). Get a chill tune with a swaying beat (kind of like the background music here) and learn your instructions to the tune. Then when you carry out the instructions move to the beat and it’s like you’re dancing them out. It’s very calming and helps both my neurological spasms disappear and my partner’s Aspergers concentration levels heighten and he de-stresses. Also what others have said regarding psilocybin (microdosing) helps Aspergers stress for people I know but it’s not for everyone. Food for thought though. Literally. Make the instructions into a song and the movements into a dance. It becomes graceful and natural and calming.
Very few channels can explain this craft right as a whole. From spore, agar, fruiting conditions and more. Even less can actually teach. Thank you for being one of the rare gems that can explain in a way many can learn. One hell of teacher. A golden standard of one if you will lmao. Mush love ✌
Instead of throwing out the one with a small spot, do the transfers then put a little h2oo on the spot then cut it out. I have also poured h2oo into the agar up to the the mycelium, wait for the bubbling to stop and pour it off. It pours off the contam with it. Have saved many agar plates like this and grown good mushrooms.
Transferring clean, snow white mycelium onto a plate of virgin agar is one of the things I enjoy most about working with mycelium. Waiting for your LC video now...thanks for helping all of us improve our skills, PGT!
@@jroc09 In mycology, there‘s ‚contam(ination)‘, and ‚iso(lation)‘. Isolating a certain growth from a clean culture can provide certain characteristics that you want to isolate, e.g. especially strong growth for fast colonization of substrate.
Just inoculated some grain jars, and I just gotta say Teacher....I have always been Interested in the thought of trying to do this myself, and it was YOUR videos that gave me the final push to actually continue and start to teach myself about my new journey in mycology! I'm completely green...and im looking forward to making the many learning mistakes I have in my short future🤣 but I just wanted to thank you for all your efforts, in making all these AWESOME videos that have taught so many like me to just stop worrying and GET INTO IT! I'm trying to not be like so many who rush and make newbie mistakes along the way with dreams of how fast and easy their first flush will be🤣 but we will see how I do...excitedly watching 10 jars of b+ on brown rice grains slowly start to turn fuzzy! I bought spores instead of a liquid culture syringe though...so I've already made my first mistake😂 sorry for the long comment, but I just needed to thank and tell you that YOU'RE the MAN! And NOW? here I am learning about agar from you! absolutely making a difference in the world my man! Mush love
I did my transfer just this past saturday. Unfortunately this video didnt come out in time for help. But all of them are doing GREAT! nice and healthy and fluffy white myc. Got some Golden Teachers and some Tidal Wave
If u put ur cups in ur oven when ur pouring ur agar an putting the lids on in the oven will cut down on contam .350 works great to blows the contam away , From mush love ❤️ The Astro way is a great channel too , Love ur style great job on ur channel an ur teaching nods like me 👍
U can put plates in the fridge and use them a month later probably even two months but for actual long term storage of mycelium in a fridge you should use culture slants
Question! Do you sterilize your agar solution and then pour into the disposable ramekins? Or do you sterilize the agar and ramekins together like in the pastywhyte tek? I assume the ramekins would melt in a PC but you never know 🤷🏻♂️
@@PhillyGoldenTeacher Oh! gee, back in my heyday (on "the forums") when I was in college I would have sent you a couple stir bars. You can get a basic kit on Amazon these days for ~$50 with plate, bars, and extractor. My only problems with LC have been ignoring gas exchange and stir bars; or these days forgetting the stir bars. I really admire you passing this knowledge on to a new generation of amateur mycologists; my hat's off to you sir.
What's this? A youtuber with affiliated links in the description that actually tells you they make a commission off of them? I'm thrilled! Love your vids.
When making an agar to agar transfer, does it matter whether the transfer is placed agar side up or agar side down? I am subscribed, how does one get to the discourse community. Outstanding video, PGT. Mush appreciated.
Hi! I just ordered a kit of lion’s mane…I want to take the spores and then eat the mushrooms, I want to know the taste and the texture!!! Then will do the agar, and then the grain n the final touch..long n nice process…one step at the time…good luck to us!!,
I notice that you don't use petri dishes covered with parafilm. Would that disposable container you use still be fine? I feel like air might get inside even when they are closed since they are made of thin plastics. Thank you for your precious postings
Yes, depending on conditions, it can take 6 weeks or longer from a spore syringe to get minor mycelial growth. This method fast-forwards you to that point.
I noc'd spores to grain 31 march, it will be ready to xfer next week. Ill give agar a shot in the next coupla weeks to test a syringe that didnt do shit.
I'm a novice, and ive learned alot from this channel. But I don't understand why multiple transfers are needed. Wouldn't that only increase the chance for contamination if the mycelium is clean after the first transfer? Or is it just likely there are contams embedded into the mycelium itself until multiple transfers of clean mycelium are done? Thanks for the educational content philly!
Hi PGT. Always love your work. Thank you! Quick one: when transferring a slice of culture from one dish to another, I notice some people put down the small wedge upside down in the second agar dish. Others put down the wedge right side up. Does it matter, and why? Kind regards, and thanks in advance.
0:19 the top corner growth is not mycelium? Also, once you make the transfer from cup 1 to cup 2, do you discard cup 2 or can use that for grain transfer as well?
Pgt! I love your channel, you’ve helped me and all of us tremendously thank you! I have a question. I followed your agar recipe, and transferred some agar from another plate just like you did in this video, and almost 2 weeks later I still have no growth in them. I think I might have not boiled the mixture long enough or I might have put too much food coloring. Would either of those be to blame?
What Are These plastic containers you are using instead of Petri dishes? It seems they also don’t require any parafilm to be sealed. Pretty handy if that works 😉
They are condiment cups for anyone else wondering. They’re pre-sterilized and don’t require parafilm. Also super cheap. Only downside is they don’t look as visually appealing when the mycelium is fully grown out as it does on traditional Petri dishes.
If you only have spots what I have done and it worked was take a small piece of paper towel, a little bigger then the spot, soak it with hydrogen peroxide, cover and let sit. I found the next day it was killed and a few days later the mycelium grew over it. If the whole thing is green spray the h2 oo on the whole thing and see what happens. At that point it can’t hurt. Let me know how it goes.
just trying out my first agar plates with some oyster spore . Mycilium is growing like wildfire although ive noticed some yellowing on a couple plates . mycilium piss or contamination ?
Hey teach great videos very interesting I’m new to the agar my question is this once you transfer to a clean plate do you just throw out the old ones even if there’s no bacterial growth
Ayyyy, do you let your poured agar sit for about 5 days or so to ensure it wasn't the agar? I'm JUST starting and there's sooo much controversy of letting it sit vs using it soon
Great videos! Question about Agar plates... Have you ever tried to sterilize your Agar with the microwave oven? I have had some luck with this method, but haven't heard of anyone doing this.
I’ve seen different opinions on isolates. Some ppl say you have to isolate and test them all out, and some people say just get it to rhizo and run with it then make clones. What do you personally do? Do I need to isolate strains to get strong flushes like yours?
It helps for sure man. Usually when you see petri dishes with rhizonorphic growth that means they either cloned a mushroom or did a few transfers. My mycelium always starts fluffy when I start at spore, then usually takes 2 transfers if you know what you are looking g for to achieve rhizomorphic mycelium. I feel like all those time lapses and stuff are already using established and isolated genetics but I'm no mycologist so I could be wrong
That small spot looked like stray mycelium.... they usually happen when there is water dripping onto your spores ill get runoff that starts mycelium on the sides of the cup, but they eventually converge and form rhizomorphic mycelium!
Great videos!!! My first batch of mycelium is ready for transfer, just wondering if there is no contamination present in a few of my agar cups do I need to transfer or can I just let those ones grow? Any help would be much appreciated.
How do you make your agar in the cups, Do you poor it and put it in the pc, I do that but I get too much moisture in the cups. What is the right way to do this. Very much appreciated for your response. Thanks.
Curious about timing. Total newbie here-sorry if it’s basic... Does a spore to grain take about the same time as a spore to agar in terms of mycelium development and growth? My guess is spore to agar works faster?
If you made the agar pots and don't have a hood would it be ok to spray some iso alcohol on it to kill bacteria and leave for storage? (With lid on).. one last thing I'm going to try some clove oil in my agar mix would that work... thanks
Why not immerse everything in alcohol especially the agar cups and lids, then quickly let dry before you pour the agar? I saw the video of working with the cups in front of the open oven on 250 degrees, but I don't think that's enough to sterilize them. Maybe that's why you got contaminated medium?
Im learning here . Is it possible to cut off a bit of mycelium thats just colonised an all in 1 grow bag and put it in a new all in 1 bag to colonised that ?
How long does a agar plate usually take to completely colonize? I inoculated played 2 weeks ago and they seem like they’re barely colonizing. They’re growing in very thin. Is it bad if I just let the agar sit there to see if it’ll colonize more ? I don’t have any contam & I’m just confused at this point. Could it possibly be bad genetics? I’m a amateur btw only 2-3 months into this hobby 😅
Also, do you know why peptone smells so bad? Literally probly one of the worst things I've ever smelt. Bacteria in rice jars has made me puke. But that peptone poop smell is something else. 😆
I don't do anything with mycology but randomly got your broke boi tek video recommended and binged this channel since. I have OCD and mild aspergers and get stressed to hell when following instructions/ "how to's" in general but something about this is just so calming on a visceral level. Thx man.
You should try some mushrooms as a therapy and report back
I agree, very cool and chill dude here.
@Aco Znas94I ...psychotic..??
Jordan Babcock Psychotic mushrooms are what you hallucinate on a bad trip? 😝
Heya. Wondered if you’d like a tip (which to be fair you might already know).
Get a chill tune with a swaying beat (kind of like the background music here) and learn your instructions to the tune. Then when you carry out the instructions move to the beat and it’s like you’re dancing them out.
It’s very calming and helps both my neurological spasms disappear and my partner’s Aspergers concentration levels heighten and he de-stresses.
Also what others have said regarding psilocybin (microdosing) helps Aspergers stress for people I know but it’s not for everyone. Food for thought though. Literally.
Make the instructions into a song and the movements into a dance. It becomes graceful and natural and calming.
Very few channels can explain this craft right as a whole. From spore, agar, fruiting conditions and more. Even less can actually teach. Thank you for being one of the rare gems that can explain in a way many can learn. One hell of teacher. A golden standard of one if you will lmao. Mush love ✌
I'm pouring my first agar in about 15 minutes. Thank you for all the videos!
Instead of throwing out the one with a small spot, do the transfers then put a little h2oo on the spot then cut it out. I have also poured h2oo into the agar up to the the mycelium, wait for the bubbling to stop and pour it off. It pours off the contam with it. Have saved many agar plates like this and grown good mushrooms.
you mean hydrogen peroxide ?
@@SaranshKarira yes
Can u record a video?
what percentage do you use? will 3% do? how about 30%?
@@ankanahumko8486 probably 3%, 30% is crazy strong and would eat through the agar itself
Transferring clean, snow white mycelium onto a plate of virgin agar is one of the things I enjoy most about working with mycelium. Waiting for your LC video now...thanks for helping all of us improve our skills, PGT!
What is the point of that I don’t get it I understand the ones that were contaminated but the clean one why would you want to transfer it?
@@jroc09 To isolate a certain growth.
What would be the benefits of that
@@jroc09 In mycology, there‘s ‚contam(ination)‘, and ‚iso(lation)‘. Isolating a certain growth from a clean culture can provide certain characteristics that you want to isolate, e.g. especially strong growth for fast colonization of substrate.
Litterally just put some agar in the pressure cooker and saw this. Perfect timing
Perfect every time
Im doing transfers off my first agar plates today. Much appreciation for all the info. Mush love. 🍄
Just inoculated some grain jars, and I just gotta say Teacher....I have always been Interested in the thought of trying to do this myself, and it was YOUR videos that gave me the final push to actually continue and start to teach myself about my new journey in mycology! I'm completely green...and im looking forward to making the many learning mistakes I have in my short future🤣 but I just wanted to thank you for all your efforts, in making all these AWESOME videos that have taught so many like me to just stop worrying and GET INTO IT! I'm trying to not be like so many who rush and make newbie mistakes along the way with dreams of how fast and easy their first flush will be🤣 but we will see how I do...excitedly watching 10 jars of b+ on brown rice grains slowly start to turn fuzzy! I bought spores instead of a liquid culture syringe though...so I've already made my first mistake😂 sorry for the long comment, but I just needed to thank and tell you that YOU'RE the MAN! And NOW? here I am learning about agar from you! absolutely making a difference in the world my man! Mush love
Really
How has you Mycology journey been?
Be prepared to fail. That's not me being horrid.
When the student is ready the master will appear. 🤟🏽👽❤♾🛸
Love the video and love this comment! I am ready then cause I have found this channel.
@@alltaira5922 same! It’s all about timing ⏱🧫🧬🍄🔥🖤
Yes 💯 !! I'm here I'm ready !!🤩 Synchronization ✨
Not only are rhizomorphic mycelium quicker to colonization of substrate but they are also more likely to create pining/fruits.
Not true homie
Its all about the conditions you have to trigger pinning.
Dont misinform homie
the most elite educational videos here. glad this is free knowledge
Casual flash of the enigma label, been trying for enigma for a while now! Awesome videos!
😁🍄
I did my transfer just this past saturday. Unfortunately this video didnt come out in time for help. But all of them are doing GREAT! nice and healthy and fluffy white myc. Got some Golden Teachers and some Tidal Wave
How do you get those flavors? Could use some spores
Where do you get the spire prints from? Also liquid culture syringes?
@@carinapalomino8069 you cant get a liquid culture syringe but you can get a spore syringe as for where to buy then go to reddit or shroomery.
I'm doing GT right now as well, is your GT mycelium fluffy on your agar like mine is?
@@sheeeitmayn4384 its been a while but yeah i had fluffy growth.
Can you please do a video demonstrating how to create/store an agar "library" ? LOVE your content sending peace and love
@PhillyGoldenTeacher I'd like to see this too! 🡹
#metoo
Thirded
I was just wondering about this
Great topic! I am interested as well
i feel like im watching some professional video on the television. i was so excited for this video!!! long live your channel bro. youre a legend
If you have left over pennicilin you can extract it and put it into your agar to prevent contamination
I used to watch the X files. Then I discovered the Agar files. Thanks so much, PGT 🤓✌🏽
Great video PGT. Once an agar cup is fully colonized, how do you store it and for how long is it good for?
If u put ur cups in ur oven when ur pouring ur agar an putting the lids on in the oven will cut down on contam .350 works great to blows the contam away ,
From mush love ❤️
The Astro way is a great channel too ,
Love ur style great job on ur channel an ur teaching nods like me 👍
Just discovered your channel. So relaxing. Thank you for the info and the vibes
You're a god among men for these tutorials thank you so much
well looks like i found out what im doing today!
I love your videos man, they are always great quality 🎥
you're an amazing person. thank you
Thanks for all the amazing videos you make growing mushrooms very fun and entertaining. I’ll try to grow my very own. Greetings from México
awesome. I'm trying to figure out how much I need to put into 10oz of broth.
Mush love, keep up the great videos
Love watching agar work 👏
How long can you keep the mycelium on agar for? in storage, like a fridge? can it be used x amount of months later?
U can put plates in the fridge and use them a month later probably even two months but for actual long term storage of mycelium in a fridge you should use culture slants
Question! Do you sterilize your agar solution and then pour into the disposable ramekins? Or do you sterilize the agar and ramekins together like in the pastywhyte tek? I assume the ramekins would melt in a PC but you never know 🤷🏻♂️
Should I put the mycelium up or down against the agar plate?
I would be very interested in a liquid culture video.
Just search for Mossy Creek Mushrooms here on YT. They go over LC in detail on their channel.
It's in the works!
All I'm gonna say is stir bars, don't forget the stir bars.
@@AaronAlso in a few months I'll mess with them. For now I just throw in a marble for my LC jars. Stir bar is a luxury for little old broke boi.
@@PhillyGoldenTeacher
Oh! gee, back in my heyday (on "the forums") when I was in college I would have sent you a couple stir bars. You can get a basic kit on Amazon these days for ~$50 with plate, bars, and extractor.
My only problems with LC have been ignoring gas exchange and stir bars; or these days forgetting the stir bars.
I really admire you passing this knowledge on to a new generation of amateur mycologists; my hat's off to you sir.
What's this? A youtuber with affiliated links in the description that actually tells you they make a commission off of them? I'm thrilled! Love your vids.
When making an agar to agar transfer, does it matter whether the transfer is placed agar side up or agar side down?
I am subscribed, how does one get to the discourse community. Outstanding video, PGT. Mush appreciated.
Thanks for the update.
@10:00 'Oh-oop" myco talk for - hope that side of the cup was sterile :-) Love your funny and educational videos sensei!
I'll be doing it soon thanks to this video. The clear closeups were very helpful.
I really need a new hobby and this is great. Can't wait to grow some lion's mane.
Hi! I just ordered a kit of lion’s mane…I want to take the spores and then eat the mushrooms, I want to know the taste and the texture!!! Then will do the agar, and then the grain n the final touch..long n nice process…one step at the time…good luck to us!!,
So glad I found your page mush love
Great video. Really have come a long way in the short amount of time you have been on youtube. Really looking forward to the genetics videos.
Would storing the agar cups upside down reduce the condensation and also limit contamination?
I notice that you don't use petri dishes covered with parafilm. Would that disposable container you use still be fine? I feel like air might get inside even when they are closed since they are made of thin plastics.
Thank you for your precious postings
What a great video! I appreciate all of your hard work and making it easy for us!
What is the harm or risks of going from a round one agar straight to grain (assuming a clean looking culture in round one)?
Could you put this leftover in your yard and it possibly grow mushrooms in the wild so to speak
Is it really speeding up the process by a week or two if it takes a week or two for the mycelium to grow?
Yes, depending on conditions, it can take 6 weeks or longer from a spore syringe to get minor mycelial growth. This method fast-forwards you to that point.
I noc'd spores to grain 31 march, it will be ready to xfer next week. Ill give agar a shot in the next coupla weeks to test a syringe that didnt do shit.
PGT is my fav shroomer!
I'm a novice, and ive learned alot from this channel. But I don't understand why multiple transfers are needed. Wouldn't that only increase the chance for contamination if the mycelium is clean after the first transfer? Or is it just likely there are contams embedded into the mycelium itself until multiple transfers of clean mycelium are done?
Thanks for the educational content philly!
Do you use anti bacterial agar from the start? Or start with MEA or water based agar first?
Hi PGT. Always love your work. Thank you!
Quick one: when transferring a slice of culture from one dish to another, I notice some people put down the small wedge upside down in the second agar dish. Others put down the wedge right side up. Does it matter, and why?
Kind regards, and thanks in advance.
0:19 the top corner growth is not mycelium? Also, once you make the transfer from cup 1 to cup 2, do you discard cup 2 or can use that for grain transfer as well?
PGT…You da’ man ☮️☮️👌👍❤️
😁🍄
Pgt! I love your channel, you’ve helped me and all of us tremendously thank you! I have a question. I followed your agar recipe, and transferred some agar from another plate just like you did in this video, and almost 2 weeks later I still have no growth in them. I think I might have not boiled the mixture long enough or I might have put too much food coloring. Would either of those be to blame?
What Are These plastic containers you are using instead of Petri dishes? It seems they also don’t require any parafilm to be sealed. Pretty handy if that works 😉
They are condiment cups for anyone else wondering. They’re pre-sterilized and don’t require parafilm. Also super cheap. Only downside is they don’t look as visually appealing when the mycelium is fully grown out as it does on traditional Petri dishes.
If you have too many successful plates for what you want, what do you do with the rest, fridge? Freezer? And for how long is it good?
Or is it wasted?
Fridge is best for long-term storage. You do not want to freeze! 😁
Hey, what do you usually do with the leftover agar with mycelium?
Top notch as always mate
Great vid thanks...
ALSO THANK YOU FOR THE SHIRT! I ♥️IT..
Glad you like it!
Really need me a flowhood. I wanna try these cups but to worried of contaminants lol. I just stick to no pour tek till I get one
My colonization turned green what did I do wrong ??? will It still pin and fruit or is it a lost cause
If you only have spots what I have done and it worked was take a small piece of paper towel, a little bigger then the spot, soak it with hydrogen peroxide, cover and let sit. I found the next day it was killed and a few days later the mycelium grew over it. If the whole thing is green spray the h2 oo on the whole thing and see what happens. At that point it can’t hurt. Let me know how it goes.
@@kicknadeadcat will do ...Thank you
Love your videos man
ALL HAIL THE GOLDEN TEACHER!
ALLLLL HAILLL!
just trying out my first agar plates with some oyster spore . Mycilium is growing like wildfire although ive noticed some yellowing on a couple plates . mycilium piss or contamination ?
Oysters sometimes have yellowish secretions and it is ok
Great tool ...thank you..
Hey teach great videos very interesting I’m new to the agar my question is this once you transfer to a clean plate do you just throw out the old ones even if there’s no bacterial growth
Thanks for this PGT
PGT- do you sterilize your agar cups? What’s your method?
Ayyyy, do you let your poured agar sit for about 5 days or so to ensure it wasn't the agar? I'm JUST starting and there's sooo much controversy of letting it sit vs using it soon
Noticed the PET 1 on the lid of the ketchup cup, which means it can not take high heat. How did you sanitize them?
Alcahol
This is mega informative.
Hey PGT, how do you store your agar dishes if you make them before youre ready use them?
Great videos! Question about Agar plates... Have you ever tried to sterilize your Agar with the microwave oven? I have had some luck with this method, but haven't heard of anyone doing this.
Love you pgt mush love 🤙🏽🍄❤️
I’ve seen different opinions on isolates. Some ppl say you have to isolate and test them all out, and some people say just get it to rhizo and run with it then make clones. What do you personally do? Do I need to isolate strains to get strong flushes like yours?
It helps for sure man. Usually when you see petri dishes with rhizonorphic growth that means they either cloned a mushroom or did a few transfers. My mycelium always starts fluffy when I start at spore, then usually takes 2 transfers if you know what you are looking g for to achieve rhizomorphic mycelium. I feel like all those time lapses and stuff are already using established and isolated genetics but I'm no mycologist so I could be wrong
That small spot looked like stray mycelium.... they usually happen when there is water dripping onto your spores ill get runoff that starts mycelium on the sides of the cup, but they eventually converge and form rhizomorphic mycelium!
Thank you
🙏
Do i absolutely have to make the transfers? What if i see no contamination?
Great videos!!! My first batch of mycelium is ready for transfer, just wondering if there is no contamination present in a few of my agar cups do I need to transfer or can I just let those ones grow? Any help would be much appreciated.
That's a good question but I'm not sure. Hope we both find out 🙂
Great vid, what kind of cups are those?
If you take a culture from an apparently healthy dish do you throw out the original dish with the healthy culture or keep to take more samples from?
TY for the VIDS 😀
PGT is mycelium a bit fuzzy? It looks great but fuzzy, some cups more than others but I took transfers from a multi spore plate.
tomentose v rhizomorphic mycelium
Why do you put the agar pots on an oven shelf??
Those cups have a recycle code of 1. How do you sterilize them and the agar?
Would you not sent that last plate as it is?
What do you do with the contaminated mycelium? Can you toss it in a compost pile with a bunch of worms? I would probably do that...
Yes you can compost it 😊👍
I’m sure the worms would love that
How do you make your agar in the cups, Do you poor it and put it in the pc, I do that but I get too much moisture in the cups. What is the right way to do this. Very much appreciated for your response. Thanks.
Curious about timing. Total newbie here-sorry if it’s basic... Does a spore to grain take about the same time as a spore to agar in terms of mycelium development and growth? My guess is spore to agar works faster?
If you made the agar pots and don't have a hood would it be ok to spray some iso alcohol on it to kill bacteria and leave for storage? (With lid on)..
one last thing I'm going to try some clove oil in my agar mix would that work... thanks
You mention it takes 1-3 transfers to get a clean dish? Is this always the case, or can you colonize a dish the first time without any contamination?
Why not immerse everything in alcohol especially the agar cups and lids, then quickly let dry before you pour the agar? I saw the video of working with the cups in front of the open oven on 250 degrees, but I don't think that's enough to sterilize them. Maybe that's why you got contaminated medium?
Very new to this about to try and run agar do you leave the portion cups or Petri dishes inside the still air box after inoculating the agar
Are you working in front of a flow hood or a still air box? I’m wondering how contams are still gettin in your agar?
Still air box
Im learning here . Is it possible to cut off a bit of mycelium thats just colonised an all in 1 grow bag and put it in a new all in 1 bag to colonised that ?
So you buy aigar powder and a colour and then how you get it to this blue liquid colour?
That last agar dish that you did the transfers from can you still reuse that
How long does a agar plate usually take to completely colonize? I inoculated played 2 weeks ago and they seem like they’re barely colonizing. They’re growing in very thin. Is it bad if I just let the agar sit there to see if it’ll colonize more ? I don’t have any contam & I’m just confused at this point. Could it possibly be bad genetics? I’m a amateur btw only 2-3 months into this hobby 😅
Amazing! Thank you for the excellent videos man!!!!!
Also, do you know why peptone smells so bad? Literally probly one of the worst things I've ever smelt. Bacteria in rice jars has made me puke. But that peptone poop smell is something else. 😆
😄🍄
Just wondering, do the lids keep out contamination as well as petri with parafilm?
Where does one get sterile agar plates ?