Thank you, as a person who is new to mushroom cultivation, I appreciate how step by step you are with your videos, you're an excellent and thorough teacher.
You don't need an injection port. put two layers of foil on the top of your jar. Lift up the first layer and inject culture or spore into the jar. It's been working fine for me for years.
@@trump45and2zig-zags make a still air box. Its hard to explain, you get a tote and make two holes for your hand and get some pvc pipe or something your hand can fit through. Glue some of those big rubber cleaning glove to the inside and seal everything up. I put glade wrap over the top of mine so I can see better. Put everything you need in, and spray some rubbing alcohol in, then put the wrap over it and let it sit a while.
My guy you are my hero. I’m starting up my company and trying to get all aspects for sale, including culture lids. Went to mass produce them and about had a stroke from how frustrated I was getting from the lids cracking. You are a god.
Pro tip. Use a one of those triangle stone bits and drill through like you would a step bit. Its just sand through so there is no risk of craking or breaking at all
I don't understand this comment. With no special effort, I've never cracked a plastic mason jar by drilling. Frankly, if I was doing a shotload (ahem) of these, I'd probably pop for a drill press and do 10 at a time.
Where do you store your liquid culture jars, A cool dark place, a Fridge…? Also how long will a jar last before it’s no longer good to use? Btw, Thanks Ashley for another perfectly narrated video!!🫶🏼
I never comment but I want to thank you for the way you instruct. There are many out there but I keep coming back to your info because it is basic in the best way possible. Sometimes it gets so confusing. Your instructions are very linear. Thanks.
If you forgot the aluminum foil also I found a really good way of keeping the coffee filters over the jars by using the original ball rim the The jar came with Nice deep and snug
10:45 it's also the perfect time to 'noc up a few more jars with that LC so you can save some time on turnaround. Instead of having to go from the tissue biopsy stage next time. Lovely video BTW! Makes me want to get growing again. So much faster than cannabis with zero electricity required and basically one brief moment of light just to start pinning? You can't beat that. I'm just super weary about trying to go myco-psycho again because the last go around I got arrested for unrelated reasons and wasn't able to return home for a few weeks. By that time I had 8 Rubbermaid tubs that were just an orgiastic death feast of aspergillus and black mold plus some contams I'd never even seen before. Ever since then I can't even keep brand new bread in this freaking apartment for 2 days without it molding. I'm terrified if I put new tubs in they'd have the same exact fate.
By doing the tissue biopsy you select the best genetics from the mycelium. A pity to hear what happend. Anyways if you esterilize properly Im sure your not going to have any problem.
You're going to need fungicide spray. Look it up, set up more than one in your case. Perhaps everywhere you put tubs in the past as well as one for the common areas. Set the sprayer up and leave the apartment for the day. Come back and you should be in better shape.
Ashley, you are AWESOME!!! I have asked you two questions, and you have answered them both. Unlike Cameron the Grain Man where I bought some grain, he did not answer me on YT or via email. And guess what, he sold me bad grain, did not spawn a bloody thing. So big huge thanks to you, what your are sharing, and doing. What you are sharing is invaluable to all of us Mycology Students.
@@BoomerShroomer I ordered five, the first was bad. I lost one bag, and the spores. I have four left, and there is no way I will try them. That is $50 down the tubes.
@@jameskreiter how do the other ones look? As long as they smell good and you can’t see contamination they should be good to use. I use his bags lot and rarely have issues.
@@BoomerShroomer They all look the same. and smelled just fine. I am afraid to even try them. Moving forward I am doing the whole process in house. At least I only have me to blame.
I bought the HaBeuniver 20mm injection ports, the shorter grey ones that seem to be very common, and they'll fall right out of a 1/2" hole but a 7/16" is a perfect fit. Thought this might save someone buying the wrong bit. A brad point bit works great on the standard metal lids, btw. Great video, Ashley, as always.
Amazon has those lids in colors, with silicone gaskets. They are sweet. I use 1/4 drill grey injection ports, and 1/2" stick on air exchange glued down with red high temp RTV sealer. They work great!
Hey Ashley, check this out, this is from a fellow named Marc R. Keith who has been growing since the 70’s, “Throwing spores into honey or karo has to be the worst possible way to make a liquid culture and I'll be glad when this current fad passes and folks can get back to established techniques. Just inoculate some grains or brf in the standard way and you'll be miles ahead. For those who wish to make LC for inoculation, here's a way to get 100 syringes full of inoculant in two weeks: 1) Inoculate a quart jar of rye berries or wbs with your spores. Upon full colonization, shake the jar to loosen each individual kernel. Be sure to give it the smell test to make sure it smells like fresh mushrooms. 2) PC a quart of distilled water in a jar with a filter disk or tyvek, etc. In a glovebox or in front of a flowhood, pour 2/3 of the quart jar of sterilized water into your jar of shaken grains. 3) Shake well and then pour the now mycelium rich water back into whatever was left of your jar of sterilized water. Use the jar lid to hold back the grains themselves from pouring out. You now have a full quart of highly concentrated mycelium water that can then be used as is, or diluted two or three times again, and used to fill syringes. Because the grains were shaken first, the mycelium is ripped into shreds and will be sucked through even small needles easily, although larger needles always work better for this. Remember, mycelium grows poorly in water unless under constant stir which oxygenates it. With grains, they colonize in two weeks or less because oxygen is throughout the jar in the spaces between the kernels. LIQUID CULTURE - A glovebox is not advanced equipment. One can be made free with stuff around the house, so there's no need for people to work in open air or fecal and mold infested bathrooms and kitchens. There is also no way to generate 100 full syringes of liquid mycelium in very small pieces within two weeks from injecting spores into honey or karo. I'm sure if people blindly used the grain method I described above, many would still have problems too, because they're inserting unproved and possibly contaminated spore solution into grains, which also isn't proper mycological technique. However, agar can easily be poured in a glovebox, and the Petri dishes used to isolate healthy mushroom mycelium away from any contaminants in less time than it takes to even know if a karo LC is contaminated or not. That agar wedge can then be used to inoculate the quart(or pint) jar of grains, that can then be turned into at least 100 syringes, or used for g2g transfer to get ten jars the old standard way. In addition, after the water is poured out of the grain jar, it can be placed back on the shelf to re-colonize, and the process can be repeated again. I just strongly feel new growers are doing themselves a disservice when they simply inject spores into honey water and then sit there waiting for something to happen. At the very least, inject directly to grains so you can visually inspect the process. Perhaps Agar's lids with injection ports will help those who have never used grains to get off to a good start.” From, “All of RR’s notes.”
The plastic lids do *_not_* seal against the jar. This may not be an issue for this process, but it's generally good for anyone using mason jars to be aware of this fact. Also, for people without a magnetic stirrer: An Oster blender (a.k.a. "Osterizer") blade assembly and sealing ring will fit a wide-mouth mason jar! Cheers from elsewhere on occupied Anishinaabe land.
Where did you store your jars?? Do you incubate at a certain temperature ?? Do you put it in the dark/ Thanks so kindly for any information !! Wonderful Video... :)
Yes, but the germination and/or growth potential of your sample will be quite low. Mycowilly posted a video recent on YT that walks through bringing dehydrated fruit “back”
I took morel culture possibly correctly, the matter that has formed is the same shade of blue Grey and the bases I plucked from the ground. I put the container outside with some holes in it bc it's suppose to be cool and moist today
I have a lot of questions about this actually. I haven’t done it before. I’d like to know about the benefit of doing it this way versus just getting a sports orange because it seems like a lot of expense and cost to do it this way and as far as how long it takes, I don’t think you’re saving any time in the long run. Also, how do you store that life culture and how long does it keep? But of course, I love your videos. They are my favorites and I always watch yours when I need to learn something new.
I’m brand new to this. Built a still air box, I have a colonized spawn bag coming. I’ll be making my own substrate. I have everything for agar plates, slants, and am wondering would it be asinine to also have LC? I’m growing lions mane as my first go.
Hi Ashley I have a newbie question. What temp do you leave your liquid culture for colonization and is it in complete darkness the whole time? And when the jar is fully colonized, how long can it be stored for?
@@nailinyourmom I thought you should store it in the dark. Is this 12 hour light cycle really necessary. I have not tried LC myself yet but will soon, so am really interested in the optimum conditions.
Liquid culture is not spores it is live mycelium Yes a syringe of culture will work but spores need to germinate into mycelium so it has to go to agar/mycelium/ then to a LC She said this a few comments prior 👍
Hey Ashley I have been watching your videos for the past months and I’m leaning towards growing some myself.. I was wondering if you can ship me some spore prints so I can try growing my own.. I have my agar ready and everything I just can’t seem to find any sites that sell spores:(
Where do you store the unused portion of the liquid culture? How long is its shelf life once its "finished". Also, when making it, where do you store the jar when it's colonizing over the course of those days?
Igrow mushrooms I've been doing it for about 10 years I would say I have no problem with infections ever on Agar. But it's a lot easier to get infections in liquid media for some reason I don't know why it just seems to be a lot easier I mean I have had probably 5050 infection to success ratio for liquid cultures that which is terrible.
Are these injection and gas release ports reusable with sterilization and where do I get them. Could probably find them but thought you might be able to let me know.
How long can liquid cultures be stored for? Must they be refrigerated? I was under the impression that fungal spores are super resilient, do they decay/die? I also realize spores and culture are different, but can someone speak to liquid spores.
This may have been asked, but the Lid MFG says do not place in a pressure cooker " If not sealed, will these lids survive the heat of a pressure cooker? A: These are not intended for use in a pressure cooker. They are just for everyday storage so that you can save your metal lids for canning. And no, this kind of plastic can't tolerate the heat of a pressure cooker. A pressure cooker reaches 250 degrees F and polypropylene plastic is rated for max temp 240 degrees Fahrenheit. Becky for Cornucopia Brand Can you provide feedback or did you use a different brand of lid?
Ive seen many say 3-6 months in dark room temp place; but u gotta check its virility every month or two on a agar plate as mycelium eventually does experience senescence.
Do you tighten jar lids or leave them loose in pressure cooker? Also do you leave liquid culture at room temperature after inoculating until sufficient growth?
First video I’ve seen that shows the initial growth, I looked for hours finding a video that didn’t show a week later when it was all cloudy and chunky and I thought my white clumps was mold
Here's a question what gauge and length are those needles where do you get those I've never seen those super long needles I've only ever used regular ones or long blunt ones but if you're going through a self healing port it's best to have something sharp or you're not getting through it
6 months later I rewatched this and had the same question and had a good laugh noticing my previous comment was exactly what I wanted to ask again. Any chance someone can answer this question on the main thread. Thanks! 🎉 great channel
Thanks for your Time Ashley. I am using Raw Honey from a local Bee Farmer, to do LC ..Great Video, and Thanks again for your Help with the Hepa Filter Math!..BtW Did I Mention Theres a Fungus umung us?! Lol! Have a Great Weekend Ashley ! PEACE!
@8:30 "a lot harder said than done?" It seemed to me that you said it just fine. It actually looked harder to do rather than to say. .. I'm just busting chops. Great video. For real.
Instead of inoculating your liquid culture from an actual fresh mushroom picked from harvest, can’t you also inoculate using mycelium that you grew on an agar petri dish? Would all the steps be the same except for the what your syringing (viz your Petri dishes’s mycelium as opposed to a freshly picked split base of a mushroom)? Thank you and great channel!
Thank you, as a person who is new to mushroom cultivation, I appreciate how step by step you are with your videos, you're an excellent and thorough teacher.
thank you!
You don't need an injection port. put two layers of foil on the top of your jar. Lift up the first layer and inject culture or spore into the jar. It's been working fine for me for years.
Any other tips for anewbie? Spores n all in 1 grow bag should be here this week
@@trump45and2zig-zags make a still air box. Its hard to explain, you get a tote and make two holes for your hand and get some pvc pipe or something your hand can fit through. Glue some of those big rubber cleaning glove to the inside and seal everything up. I put glade wrap over the top of mine so I can see better. Put everything you need in, and spray some rubbing alcohol in, then put the wrap over it and let it sit a while.
@@jesush.christ5978 like a sandblaster, I gotcha, thanks. Excited to grow my first batch when I get everything this week
N you pretty much just make a foil pouch with the jar, right?
@@trump45and2zig-zags are you using jars and doing the brown rice flour tek?
Pro tip for anyone doing this for the first time, always run your drill in reverse when drilling plastics to prevent cracking.
@Valerian Roots Yup, if you're drilling plastic, no matter the size of the hole, run the drill in reverse and don't push to hard. Works every time.
My guy you are my hero. I’m starting up my company and trying to get all aspects for sale, including culture lids. Went to mass produce them and about had a stroke from how frustrated I was getting from the lids cracking. You are a god.
Pro tip. Use a one of those triangle stone bits and drill through like you would a step bit. Its just sand through so there is no risk of craking or breaking at all
Or use a drill & brad point bits...
I don't understand this comment. With no special effort, I've never cracked a plastic mason jar by drilling. Frankly, if I was doing a shotload (ahem) of these, I'd probably pop for a drill press and do 10 at a time.
Wow! Thank you! This was by far the most thorough step by step video I've found so far, and I've watched probably a dozen or more
Where do you store your liquid culture jars, A cool dark place, a Fridge…? Also how long will a jar last before it’s no longer good to use? Btw, Thanks Ashley for another perfectly narrated video!!🫶🏼
Once growth slows down in the jar, you can store it in the fridge for up to 4 months.
I've made mine and put it straight in the fridge can I take it out and let it grow a bit and put it back?
This is probably the best instructional video I've ever seen!!!! Thank you!!
I never comment but I want to thank you for the way you instruct. There are many out there but I keep coming back to your info because it is basic in the best way possible. Sometimes it gets so confusing. Your instructions are very linear. Thanks.
If you forgot the aluminum foil also I found a really good way of keeping the coffee filters over the jars by using the original ball rim the The jar came with Nice deep and snug
10:45 it's also the perfect time to 'noc up a few more jars with that LC so you can save some time on turnaround. Instead of having to go from the tissue biopsy stage next time. Lovely video BTW! Makes me want to get growing again. So much faster than cannabis with zero electricity required and basically one brief moment of light just to start pinning? You can't beat that. I'm just super weary about trying to go myco-psycho again because the last go around I got arrested for unrelated reasons and wasn't able to return home for a few weeks. By that time I had 8 Rubbermaid tubs that were just an orgiastic death feast of aspergillus and black mold plus some contams I'd never even seen before. Ever since then I can't even keep brand new bread in this freaking apartment for 2 days without it molding. I'm terrified if I put new tubs in they'd have the same exact fate.
By doing the tissue biopsy you select the best genetics from the mycelium. A pity to hear what happend. Anyways if you esterilize properly Im sure your not going to have any problem.
You're going to need fungicide spray. Look it up, set up more than one in your case. Perhaps everywhere you put tubs in the past as well as one for the common areas. Set the sprayer up and leave the apartment for the day. Come back and you should be in better shape.
Thank you immensely. I like your video. As a beginner. I have to watch it over and over again.
The best video on the subject that I've seen. Thank you.
Wow, thank you! I appreciate it. 🍄❤️🍄
Ashley, you are AWESOME!!! I have asked you two questions, and you have answered them both. Unlike Cameron the Grain Man where I bought some grain, he did not answer me on YT or via email. And guess what, he sold me bad grain, did not spawn a bloody thing. So big huge thanks to you, what your are sharing, and doing. What you are sharing is invaluable to all of us Mycology Students.
Thank you. Did Cameron replace your bags? How many were bad?
@@BoomerShroomer I ordered five, the first was bad. I lost one bag, and the spores. I have four left, and there is no way I will try them. That is $50 down the tubes.
@@jameskreiter how do the other ones look? As long as they smell good and you can’t see contamination they should be good to use. I use his bags lot and rarely have issues.
@@jameskreiter I’d be happy to refund you your order from them.
@@BoomerShroomer They all look the same. and smelled just fine. I am afraid to even try them. Moving forward I am doing the whole process in house. At least I only have me to blame.
Knowing you can grow LC from biopsy instead of spores helped me a lot! Thank you.
Hello. Excuse me
can you make champignon mushroom seeds ??
i want to learn to make it
I bought the HaBeuniver 20mm injection ports, the shorter grey ones that seem to be very common, and they'll fall right out of a 1/2" hole but a 7/16" is a perfect fit. Thought this might save someone buying the wrong bit. A brad point bit works great on the standard metal lids, btw. Great video, Ashley, as always.
Amazon has those lids in colors, with silicone gaskets. They are sweet. I use 1/4 drill grey injection ports, and 1/2" stick on air exchange glued down with red high temp RTV sealer. They work great!
What’s the lids called?
Is that an injection Port filter? I have a question about injection ports when you tip the jar over doesn't the filter get wet?
Hey Ashley, check this out, this is from a fellow named Marc R. Keith who has been growing since the 70’s,
“Throwing spores into honey or karo has to be the worst possible way to make a liquid culture and I'll be glad when this current fad passes and folks can get back to established techniques. Just inoculate some grains or brf in the standard way and you'll be miles ahead. For those who wish to make LC for inoculation, here's a way to get 100 syringes full of inoculant in two weeks: 1) Inoculate a quart jar of rye berries or wbs with your spores. Upon full colonization, shake the jar to loosen each individual kernel. Be sure
to give it the smell test to make sure it smells like fresh mushrooms. 2) PC a quart of distilled water in a jar with a filter disk or tyvek, etc. In a glovebox or in front of a flowhood, pour 2/3 of the quart jar of sterilized water into your jar of shaken grains. 3) Shake well and then pour the now mycelium rich water back into whatever was left of your jar of sterilized water. Use the jar lid to hold back the grains themselves from pouring out. You now have a full quart of highly concentrated mycelium water that can then be used as is, or diluted two or three times again, and used to fill syringes. Because the grains were shaken first, the mycelium is ripped into shreds and will be sucked through even small needles easily, although larger needles always work better for this. Remember, mycelium grows poorly in water unless under constant stir which oxygenates it. With grains, they colonize in two weeks or less because oxygen is throughout the jar in the spaces between the kernels.
LIQUID CULTURE - A glovebox is not advanced equipment. One can be made free with stuff around the house, so there's no need for people to work in open air or fecal and mold infested bathrooms and kitchens.
There is also no way to generate 100 full syringes of liquid mycelium in very small pieces within two weeks from injecting spores into honey or karo. I'm sure if people blindly used the grain method I described above, many would still have problems too, because they're inserting unproved and possibly contaminated spore solution into grains, which also isn't proper mycological technique. However, agar can easily be poured in a glovebox, and the Petri dishes used to isolate healthy mushroom mycelium away from any contaminants in less time than it takes to even know if a karo LC is contaminated or not. That agar wedge can then be used to inoculate the quart(or pint) jar of grains, that can then be turned into at least 100 syringes, or used for g2g transfer to get ten jars the old standard way. In addition, after the water is poured out of the grain jar, it can be placed back on the shelf to re-colonize, and the process can be repeated again. I just strongly feel new growers are doing themselves a disservice when they simply inject spores into honey water and then sit there waiting for something to happen. At the very least, inject directly to grains so you can visually inspect the process. Perhaps Agar's lids with injection ports will help those who have never used grains to get off to a good start.”
From, “All of RR’s notes.”
Very nice personality , something feels relaxing and refreshing they way she teaches 😊
Any tips for making a flow hood? Where do you get supplies? Thanks!
Always grateful for the info as well as your optimistic attitude
Thank you for your kind words! :-)
Love that you use unbleached filters!
Thank you!
Hi madam Ashley,thanks for your video I have lent especially the way you find messenger and then innoculate to dishes
The plastic lids do *_not_* seal against the jar. This may not be an issue for this process, but it's generally good for anyone using mason jars to be aware of this fact. Also, for people without a magnetic stirrer: An Oster blender (a.k.a. "Osterizer") blade assembly and sealing ring will fit a wide-mouth mason jar! Cheers from elsewhere on occupied Anishinaabe land.
I love the way you hold the jar to get good angles on the pieces, you can see the growth off tot the side of the first little lump of mush
this is the best tutorial so far! thank you so much!!!
What size needle is that?
Where did you store your jars?? Do you incubate at a certain temperature ?? Do you put it in the dark/ Thanks so kindly for any information !! Wonderful Video... :)
Very informative and well-made video. Top Quality! Thank you.
The most beautiful channel ever
I have a question
1 what kind of mashroom is this
2 is this one for magic mushroom ?
👀👀👀👀👀
Great teacher!
Thank you so much for sharing your knowledge. 🙌
Where do you get those long needles to suck out the lc from jar?
This is really good sister. I like all the comments as well. Truly- I've been looking for the right videos.
I'm so glad! Thank you!
Liquid culture is definitely the way to go.
Is it possible to get spores and create a culture from dried out dehydrated mushrooms?
Yes, but the germination and/or growth potential of your sample will be quite low. Mycowilly posted a video recent on YT that walks through bringing dehydrated fruit “back”
Hey what kind of mushrooms where those they where absolute monster good genes for sure
I bought the half inch Forester drill bit and it works great it doesn't leave those nasty burrs
Does it matter what kind of grain? And also how would I sterilize the grain without having to buy special bag from online
thanks for the links, makes it so easy, love it
I took morel culture possibly correctly, the matter that has formed is the same shade of blue Grey and the bases I plucked from the ground. I put the container outside with some holes in it bc it's suppose to be cool and moist today
Can I store the LC mason jars in the fridge?
Good question 🤔
What do you do if you are using a spore print instead ?
Same technique except just scrape the spores into the jar under a SAB or in front of flow hood, sit back and watch it grow
THANKS ASHLEY! AWESOME VID!
hi , really like your videos
just wondering how and how long can you store your bottle of liquid culture
I have a lot of questions about this actually. I haven’t done it before. I’d like to know about the benefit of doing it this way versus just getting a sports orange because it seems like a lot of expense and cost to do it this way and as far as how long it takes, I don’t think you’re saving any time in the long run. Also, how do you store that life culture and how long does it keep?
But of course, I love your videos. They are my favorites and I always watch yours when I need to learn something new.
I’m brand new to this. Built a still air box, I have a colonized spawn bag coming. I’ll be making my own substrate. I have everything for agar plates, slants, and am wondering would it be asinine to also have LC? I’m growing lions mane as my first go.
Hi Ashley I have a newbie question. What temp do you leave your liquid culture for colonization and is it in complete darkness the whole time? And when the jar is fully colonized, how long can it be stored for?
@@nailinyourmom I thought you should store it in the dark. Is this 12 hour light cycle really necessary. I have not tried LC myself yet but will soon, so am really interested in the optimum conditions.
Great Tips 😊 coffee filter the LC
Hey Ashley what gauge needle do you use to extract the LC then of course, inoculate the fresh grain jar please?
I see blunt and Sharpe needles
Thanks.
Gracias Ashley. Muy clara y didáctica tu explicación. Me diste mucha información útil!
Thank you 🙏🏾. You’re awesome
So wat if I don’t have a muchroom to get it out of so I can grow wear can I get it from
Chicken or the egg situation. I hear you on that!
Hi Ashley great video I’m about to do my first LC project. Please tell me where you got your syringe and looks like a biopsy needle. Thank you
Can I use some left over liquid spores to inoculate the liquid spore culture? Great videos! Keep em coming!
This is my question! Did you find an answer?? Thx!
Liquid culture is not spores it is live mycelium
Yes a syringe of culture will work but spores need to germinate into mycelium so it has to go to agar/mycelium/ then to a LC
She said this a few comments prior 👍
If you're using hydrophobic GE filters you don't need to use foil for your lids. Leaves you plenty for spore prints! Mush love Boomer Shroomer!
Cool thanks!
What is hydrophobic GE filters? Is that the filter she puts onto the white lid?
Hey Ashley I have been watching your videos for the past months and I’m leaning towards growing some myself.. I was wondering if you can ship me some spore prints so I can try growing my own.. I have my agar ready and everything I just can’t seem to find any sites that sell spores:(
Did you start growing yet?
Where do you store the unused portion of the liquid culture? How long is its shelf life once its "finished". Also, when making it, where do you store the jar when it's colonizing over the course of those days?
Love your technique and explaining style. Very good
Thank you so much!
Even your voice, so good videos lady !keep them up !
Thank u for your kind of educate culture.
Thank you!
Please with this method can I use honny Instead of agar powder?
Another fantastic video! Very well presented and easy to learn from. I will definitely be giving this a go in the near future.
Awesome, thank you!
Ashley. You’re the best. Love your channel. What gauge and length of syringes are you using. Mine seem small and these look best for liquid culture
@@chuckcharlebois4199 use draw up needles
Igrow mushrooms I've been doing it for about 10 years I would say I have no problem with infections ever on Agar. But it's a lot easier to get infections in liquid media for some reason I don't know why it just seems to be a lot easier I mean I have had probably 5050 infection to success ratio for liquid cultures that which is terrible.
Where do you get the long syringe needles?
So professional!
Great video! Loved it. This was one of the better explained ones.
Glad it was helpful!
Are these injection and gas release ports reusable with sterilization and where do I get them. Could probably find them but thought you might be able to let me know.
How long will the liquid culture stay good? How do you store it thanks
How long can liquid cultures be stored for? Must they be refrigerated?
I was under the impression that fungal spores are super resilient, do they decay/die?
I also realize spores and culture are different, but can someone speak to liquid spores.
This may have been asked, but the Lid MFG says do not place in a pressure cooker " If not sealed, will these lids survive the heat of a pressure cooker?
A: These are not intended for use in a pressure cooker. They are just for everyday storage so that you can save your metal lids for canning.
And no, this kind of plastic can't tolerate the heat of a pressure cooker. A pressure cooker reaches 250 degrees F and polypropylene plastic is rated for max temp 240 degrees Fahrenheit.
Becky for Cornucopia Brand
Can you provide feedback or did you use a different brand of lid?
Are these golden teachers?
I have a question! If your pressure cooker only reaches 12 psi will that be good enough for LC? I've heard it's good enough for brf jars
How long can you keep the jar and the lids full of stuff and should they be placed somewhere cold like in a fridge?
Ive seen many say 3-6 months in dark room temp place; but u gotta check its virility every month or two on a agar plate as mycelium eventually does experience senescence.
How do you recommend storing the liquid culture or the agar dish if we wish to keep it? Thank you!
refrigerate it
I was wondering can you run this unit without the optional stand? Thank you for your time
Do you tighten jar lids or leave them loose in pressure cooker? Also do you leave liquid culture at room temperature after inoculating until sufficient growth?
super informative (no jadijadijada) !
Thank you!
How do you keep the culture alive during storage? Do you need to feed it?
Is it the brown rice that you inoculated? Thank you for beautiful video again.
First video I’ve seen that shows the initial growth, I looked for hours finding a video that didn’t show a week later when it was all cloudy and chunky and I thought my white clumps was mold
Nice! It is hard to see contamination in LC. It is always best to test on agar.
Here's a question what gauge and length are those needles where do you get those I've never seen those super long needles I've only ever used regular ones or long blunt ones but if you're going through a self healing port it's best to have something sharp or you're not getting through it
So glad i saw another LC look beige, then being told the mycelium will feed on that... Almost threw mine out.
do you leave the stir stick in the jar before pressure cooking it? or take it out?
6 months later I rewatched this and had the same question and had a good laugh noticing my previous comment was exactly what I wanted to ask again. Any chance someone can answer this question on the main thread. Thanks! 🎉 great channel
Thanks for your Time Ashley. I am using Raw Honey from a local Bee Farmer, to do LC ..Great Video, and Thanks again for your Help with the Hepa Filter Math!..BtW Did I Mention Theres a Fungus umung us?! Lol! Have a Great Weekend Ashley ! PEACE!
Thank you!
R u using 4% honey and distilled water for ur median?
@@jasonpelton7252 good luck it worked very well 4 me!
Really good work i had many troubles with my spawn bags... because it was always too hot, i think ur doint this since a while xD
So thorough, thank you
The air port things. What do they do and how does it work? Does it allow oxygen into the jar? Or does it only allow co2 to expell?
@8:30 "a lot harder said than done?" It seemed to me that you said it just fine. It actually looked harder to do rather than to say. .. I'm just busting chops. Great video. For real.
There's no link in the description to your new UA-cam channel and when I tried to search it I couldn't find you
Instead of inoculating your liquid culture from an actual fresh mushroom picked from harvest, can’t you also inoculate using mycelium that you grew on an agar petri dish? Would all the steps be the same except for the what your syringing (viz your Petri dishes’s mycelium as opposed to a freshly picked split base of a mushroom)? Thank you and great channel!
It should work as long as youre using sterile technique. So, yeah
@@merct2894 thanks
Thanks Ashley!
Thank you!
how long will this stay viable for use? Provided of course no contamination?
what temp for LC and dishes? good video, i learned a lot; it gave me confidence, Thank You!
Great simple :) What size for the airlock?
Hello. Please do a video on mushroom tissue culture
Brilliant video well explained 😁
Glad you liked it!
easy to follow thank you.
Love it big thanks Lods 🍄🍄👍
Thank you for the educational content. Love from Canada :)
Thank you!! 😊
Is live culture tissue necessary? Can we just add spores into the culture medium?
What size needle are you using for the biopsies? Or what brand needles do you buy 😁