DNA ligase
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- Опубліковано 23 лис 2013
- This lecture about DNA ligase explains how does DNA ligase works. shomusbiology.com/
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In molecular biology, DNA ligase is a specific type of enzyme, a ligase, (EC 6.5.1.1) that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV) may specifically repair double-strand breaks (i.e. a break in both complementary strands of DNA). Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template,[1] with DNA ligase creating the final phosphodiester bond to fully repair the DNA.
DNA ligase has applications in both DNA repair and DNA replication (see Mammalian ligases). In addition, DNA ligase has extensive use in molecular biology laboratories for recombinant DNA experiments (see Applications in molecular biology research). Purified DNA ligase is used in gene cloning to join DNA molecules together to form recombinant DNA.
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Thanks for your thorough explanations. Im a 4th year at UCSD taking a biochem class called Biosynthesis of Macromolecules and your videos are helping me a lot! Just came across this and it was really helpful. Im about to check out all your biochem videos. Thanks for posting these
As always u are reason of my relief from exam stress...
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Very good explanation about the difference between "nick" and "gap". Thank you very much!
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In book I find difficult to learn .You explained well .May you live long and keep up with good work
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sir i am a student of 12th class and today i had a presentation on DNA polymerase 3 and dna ligase. i saw your videos last evening i got the concepts very clear .My professor got stunned ..The best day of my life just because of you...i love you sir.
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very well explained :) cleared all doubts....god bless you sir :)
+chainika gupta thank you.
You have explained deeply and in detail and understandably😊
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thank you for this video! anyway has that enzyme atp complex Quatern structure? :)
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thanks for every thing i m following u from my bsc 1st year...now i m persuing msc......ur lactures r really helpful....plz a live session
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Awesome sir ji.
Your videos are always simple and easy to understand.
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Can someone tell me why there would only be 1x Phosphate left on the 5' end after Okazaki Formation? Something is stuck in my head.. thx
Sir your videos are great.
My book there is a question to write about the formation of DNA ligase what should be the answer please reply
thank you so much .. i am doing masters in microbiology subject. i must say you are life Savior.. i have first paper of molecular biology but its too difficult for me to understand, but because of you its little bit easy for me
once again thank you .. and lots of love
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hats off to u sir now i can surely pass my MSC exam. thank you
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Zazakhallah khairan.
It's very helpful video sir
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sir can you give the study material for dna ligase mechanism...also it would be really helpful in other videos too if you provide study materials for the topic :) tysm your videos help me alot
sir how will i get all molecular biology videos in sequence?? its quite confusing
Awesome explanation👍
Wow thanks that's a clear explanation
I couldn't locate a video that explained this subject better.
Thank you
sir i need the clear difference between polynucleotidal kinase and dna ligase pls sir can u clear my doubt
Why does a phosphorus molecule need to be emitted out for a phosphodiester bond to form?
Tq for all videos sir for my exam quick preparation
sir its was an beautifully illustrated explanation , but I have a doubt. How does the process of unwanted self ligation of a cleaved plasmid vector in rDNA technology, take place, without the interference of ligase enzyme?
+Sai Sarada Mythili Kothapalli thank you
very simple and interesting way to describe.
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Sir, Which book to follow for Molecular biology.
Can you share the detail mechanism video here
great video my dude
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+zoni Zoni thank you. Glad you liked my lectures
Please make a video of polynucleotide kinase and alkaline phosphatase... Asap... Please.....
please upload a video on DNA purification
Very well explained.
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Thank's it is very useful
Thank you a lot your video,
Very good explanation thank you so much sir
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Sir pls give the name of the phosphate you are saying at 11:37
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amazing explaination Sir
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Sir please make a video on homopolymer tailing technique.....as soon as possible you are the best sir God bless you always
Okay
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Oooooooooooooooooohhhhhhhhhhhhhhh now i get it!
please tell poly nucleotide kinases
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Thanks sir, it was helpful
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well done buddy thanks
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Your give your all with full hard working I hope I will watch all of your genetics related videos
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Nice videos keep it up
Thank you so much sir
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Sir please make a video on dna modifying enzyme kinase please please
When there's a gap, how are nucleotides added?
+Amna Zaim with normal DNA polymerization process with polymerase enzyme
Zazakallah khairan.
Thank you sooooooo much sir🙏🙏🙏🙏🙏🙏🙏
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Explain in hindi
Y?
So lag. Bad. Not interesting
Can you share the detail mechanism video here
Okay