Thank you very much for saying that. I'm glad you found my explanation useful. Even though the radioactive DNA method isn't really used anymore, it is still used in teaching.
This is an amazing video. I've seen multiple videos on DNA fingerprinting and this is the only one that manages to answer all my curious high school level questions without being too technical to fry my brain. Glad you're still uploading videos. Hugely underrated. Subscribed.
Thank you very much. I am actually an educator in the UK, and so I try to make my videos understandable by as many people as possible. I'm glad you enjoyed it, and thank you very much for subscribing
Thank you, I thought I'd go back to my biological roots for a video. Biology is my comfort zone I suppose. For quite a few years, there was only 1 lab in the world doing DNA fingerprints.
Ethidium Bromide is a dye that fluoresces in the presence of DNA. It is usually used to make the band more easy to see. It means you don't have to bother with the radioactive DNA probes.
I don’t really get the part where the pieces of complementary DNA are made radioactive. Can you elaborate on that? So the pieces of DNA added are complementary to the DNA strand we used as sample?
Yes I use pieces of DNA that I know to be complementary to the sequences in the sample. These pieces of DNA are made artificially and are made to be radioactive. We do the same thing today with pieces of DNA that have dye molecules attached to them. It just helps us to see where our DNA samples ended up. I hope that helps.
Interesting question. I'm not entirely sure. You need to make sure that a cut is made before the STR and then again after the STR. As long as the cuts are made in the same place in everyone's DNA, then it is just the length of the STR that differs.
Sir after gelectrophoresis if both semple has diffrent arrangement of DNA fragments then here why we can not decide both DNA semple are of different persons????.... Why we go further and perform autoradiography and all ???????
The method I discussed is just one way of making the fragments visible. We need to make them visible to see if there are any differences. This is just one way, and it's the way that's usually taught in schools (in the UK at least). I hope that makes sense
thank you so much this was very beneficial but i wanted to ask on which basis is the radioactive probe chosen, or to be clearer, what is the specific DNA sequence that i want it to be hybridized by this probe and why?
Why don't we stop after electrophoresis, isn't there a dye that is added to the fragments during electrophoresis. I mean when is it necessary to continue to jeffreys technique?
That's a good question, and the answer I don't really know, but I have some ideas. Firstly this is the technique that is often used in education. Also having seen a number of these gels, they lose water and shrivel with time. This would alter the gel and potentially make them unusable. Also I'm not sure how stable. There are solutions that do use dye (I've used some myself). Sadly when they dry out they become unusable. Hope that helps.
That's a really interesting question, thank you. I'm not sure why the sequences are found in narrow bands, but since they are non-coding they would not be affected by mutations that add extra copies of the sequences (these can happen at any time). Also quite a few of them are rich in A and T, but not all of them. The STR called FGA has the sequence CTTT, but it does appear that a lot of them have A and T. I'm not sure why, but excellent question.
Ooh interesting, I didn't know that, thank you. I'm a teacher rather than a research biologist and the radioactive probe technique is still taught. I know we don't use the RFLP analysis anymore but I thought it was interesting so I included it in the video. Whenever I've done a gel electrophoresis I've always used dyed probes, but I always assumed they were just used for education. Interesting to learn something new, thank you.
I watched 3 to 4 other videos to understand DNA fingerprinting but none of them cleared the topic as u did thanksssss
Thank you very much for saying that. I'm glad you found my explanation useful. Even though the radioactive DNA method isn't really used anymore, it is still used in teaching.
I have tried multiple times to understand from our NCERT textbook but everytime i failed. Thank you so much for explaining in a very simple way.
Finally !! An explanation of how a DNA sample can be transformed into a DNA profile ! Thank you so much.
This is an amazing video. I've seen multiple videos on DNA fingerprinting and this is the only one that manages to answer all my curious high school level questions without being too technical to fry my brain. Glad you're still uploading videos. Hugely underrated. Subscribed.
Thank you very much. I am actually an educator in the UK, and so I try to make my videos understandable by as many people as possible. I'm glad you enjoyed it, and thank you very much for subscribing
Same here
The world has received a wonderful gift. Undeniable evidence will upset many corruptible personalities. Thank you Sir Alec.
This video is awesome! I had great trouble understanding DNA fingerprinting. Now, I've got it and find it really fascinating
First time ever being able to understand DNA profiling in a much better way after watching a number of videos finally
Sir, your explanation is simple and so easy to understand. Especially for students in a resource poor set up. Thank you so much.
I did a gel electrophoresis lab in my cell biology lab. Took us three weeks to complete. Very cool.
Why does this channel not have more subscribers? Thank you for a great piece of content!
Thank you very much for the lovely comments.
3D visualisation is the best way of teaching so that all can get it very easily... Your explanation is amazing... Keep it up🎉🎉
Oh my goodness me. I can't believe this is a 2k sub channel and such professional video
Excellent !!!!!!!🙌🏻 None can forget this topic after watching this video. Very well explained 👌🏻
Thank you very much for such a lovely comment. I'm glad you enjoyed it.
Loved the Doctor Who reference! Great video! Thank you for doing this. It helped me pass my class. :)
Thank you so much. I normally need very detailed explanation with visual and this is perfect. 😊
4:20 how do we make these probes?
...i.e how do we know they're gonna be complementary to the sample dna pieces??
I saw tooo many videos of dna fingerprinting. But this one is what I was looking for❤️
Thank you very much for your lovely comment
And finally here it is.. This still helps a lot for me. Thank you so much sir for your wonderful explanation.
Great explain ❤️ love from India 🇮🇳
Man, you did a great job here. Thanks.
Thank you very much. I'm glad that you enjoyed the video.
I have been looking for video like this so long！！thankyouuuuuu！
Good, I'm glad you found what you were looking for. Thank you for watching.
Great video/explanation. Now I know why it's expensive to do DNA fingerprinting.
Thank you, I thought I'd go back to my biological roots for a video. Biology is my comfort zone I suppose. For quite a few years, there was only 1 lab in the world doing DNA fingerprints.
Excellent video once again. ❤️❤️❤️👏👏👏🔥🔥🔥
Thanks very much. I'm so glad you enjoy my videos. Thank you.
Thanku so much it is the bestt explanation on DNA fingerprinting💕
Loved the explanation and Doctor Who reference. 😂
great explanation, other youtube videos were too vague
Very well explained.
Do we use EtBr in gel electrophoresis step of fingerprinting? If not then what is the reason of it?
Ethidium Bromide is a dye that fluoresces in the presence of DNA. It is usually used to make the band more easy to see. It means you don't have to bother with the radioactive DNA probes.
I don’t really get the part where the pieces of complementary DNA are made radioactive. Can you elaborate on that? So the pieces of DNA added are complementary to the DNA strand we used as sample?
Yes I use pieces of DNA that I know to be complementary to the sequences in the sample. These pieces of DNA are made artificially and are made to be radioactive. We do the same thing today with pieces of DNA that have dye molecules attached to them. It just helps us to see where our DNA samples ended up. I hope that helps.
@@LearningCurveScience Oh, thank you so much! I understand now!
You are great at explaining
Thank you, I'm glad you think so. DNA is such a fascinating molecule.
is there any relation between the restriction enzymes used in RFLP and location of STRs?
Interesting question. I'm not entirely sure. You need to make sure that a cut is made before the STR and then again after the STR. As long as the cuts are made in the same place in everyone's DNA, then it is just the length of the STR that differs.
Your videos are really helpful!
Thank you very much. I'm glad you enjoy them.
thank you for this amazing video from Türkiye
Very simply explained,thank you sir
Sir after gelectrophoresis if both semple has diffrent arrangement of DNA fragments then here why we can not decide both DNA semple are of different persons????.... Why we go further and perform autoradiography and all ???????
We can see DNA arrangement in gel electrophoresis strp by stanning with ethidium bromide and UV rays ...????
The method I discussed is just one way of making the fragments visible. We need to make them visible to see if there are any differences. This is just one way, and it's the way that's usually taught in schools (in the UK at least). I hope that makes sense
@@LearningCurveScience ok sir love from India ❤️
thank you so much this was very beneficial
but i wanted to ask on which basis is the radioactive probe chosen, or to be clearer, what is the specific DNA sequence that i want it to be hybridized by this probe and why?
You dont need to tag the bases, for example it can pair with the Phosphate or sugar Rests every DNA has.
Why don't we stop after electrophoresis, isn't there a dye that is added to the fragments during electrophoresis. I mean when is it necessary to continue to jeffreys technique?
That's a good question, and the answer I don't really know, but I have some ideas. Firstly this is the technique that is often used in education. Also having seen a number of these gels, they lose water and shrivel with time. This would alter the gel and potentially make them unusable. Also I'm not sure how stable. There are solutions that do use dye (I've used some myself). Sadly when they dry out they become unusable. Hope that helps.
@@LearningCurveScience oh thanks so much
Sir the repeative sequence which are found in the fragments why they are only present in narrow band which are rich in A T content 🤷🏻♀️
That's a really interesting question, thank you. I'm not sure why the sequences are found in narrow bands, but since they are non-coding they would not be affected by mutations that add extra copies of the sequences (these can happen at any time). Also quite a few of them are rich in A and T, but not all of them. The STR called FGA has the sequence CTTT, but it does appear that a lot of them have A and T. I'm not sure why, but excellent question.
Back to class. I’ll get a c- w all science classes. -an MA + writer
is the same analysis we do to identify the parents ?
Yes it is, it works on the same principle.
Your voice make me feel like I'm watching a movie
2:02 is epic!
Thank you I'm glad you enjoyed the video
The radioactive method has not been used since 1995 or so. So this video outdated some 25 years ago.
Ooh interesting, I didn't know that, thank you. I'm a teacher rather than a research biologist and the radioactive probe technique is still taught. I know we don't use the RFLP analysis anymore but I thought it was interesting so I included it in the video. Whenever I've done a gel electrophoresis I've always used dyed probes, but I always assumed they were just used for education. Interesting to learn something new, thank you.
Thank you very much It was very helpfull
gr8 video mate
Thank you very much, I'm glad you enjoyed it.
Wonderful 👍
Now i understood
Thanks!
thank you for the video
You're welcome. I'm glad you enjoyed it.
thank you so mush 😭 thank you thank you soooo much , allah bless you ❤
Fucking deep hard next level explaining man 🏆🔥🙏
Thank you very much, I'm glad you enjoyed it
Thank you so much
Thank you❤❤
U r wonderful buddy.
Thank you, I'm glad you enjoyed the video
Awsomeeeeeeee
Thank you very much, that's very kind
excellent
Thanks sir
Doctor who reference!
I am a massive Doctor Who fan and geek. My time travel is full of Doctor Who references (and I do mean full)
wow ❤
DNA is awesome, I find it fascinating. Thank you for watching the video.
You sound.... Familiar. 🤔
Woah mannnn
UA-cam refused my like