Types of restriction enzyme

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Naturally occurring restriction endonucleases are categorized into four groups (Types I, II III, and IV) based on their composition and enzyme cofactor requirements, the nature of their target sequence, and the position of their DNA cleavage site relative to the target sequence.[29][30][31] All types of enzymes recognize specific short DNA sequences and carry out the endonucleolytic cleavage of DNA to give specific fragments with terminal 5'-phosphates. They differ in their recognition sequence, subunit composition, cleavage position, and cofactor requirements,[32][33] as summarised below:
Type I enzymes (EC 3.1.21.3) cleave at sites remote from recognition site; require both ATP and S-adenosyl-L-methionine to function; multifunctional protein with both restriction and methylase (EC 2.1.1.72) activities.
Type II enzymes (EC 3.1.21.4) cleave within or at short specific distances from recognition site; most require magnesium; single function (restriction) enzymes independent of methylase.
Type III enzymes (EC 3.1.21.5) cleave at sites a short distance from recognition site; require ATP (but do not hydrolyse it); S-adenosyl-L-methionine stimulates reaction but is not required; exist as part of a complex with a modification methylase (EC 2.1.1.72).
Type IV enzymes target modified DNA, e.g. methylated, hydroxymethylated and glucosyl-hydroxymethylated DNA

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[@AmitSingh-go1vg]

Sir please define role of Sam ( s adenosyl methylase) in restriction endonuclease 1 Activity

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[@mirafshana5000]

sir..in which of the system...restriction and modification activities act independently????

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i enjoyed this video! i have watched several videos recently but you really provided the base for better understanding restriction enzymes! thanks

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[@aninditapaul256]

Sir, why the host dna is methylated by restriction endonuclease type 1?

[@saher4680]

Restriction site and recognition site means the same or different? please clear this

[@nishakapase7918]

Sir plz provide the information about exonuclease enzymes and their types.
Please

[@farahquadri6385]

Kindly explain about palindromic sequences are they present in recogition and restriction site both?

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It helped me a lot
Keep posting more such videos

[@ssss...7896]

sr muje english clearly smj ni atti h...so please apse ik question h...kya R.E forgien genome m b modification krte h..ya keval apne hi genom m..

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[@wasimhaider8901]

*Hello Sir, Name the Four Nuclease Enzymes Each For Cut the Sticky End At 5prime And 3prime Ends And Four Enzymes That Cut At The Blunt End*

[@abcd-gl7ur]

I have a question... Are all these types have both modification and cleavage functions but only type 2 can do it both functions at the same time

[@jeenuanbu1333]

Thank you very much sir..bt one doubt sir in our book type 2s enzyme was not mentioned..

[@hafsailyasmuhammadilyas1421]

very informative and helpful for me

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[@zayanali2881]

Sir! will you please tell me that palindromic sequence and recognition sequence is same thing or different.

[@arijitpalit2756]

No, palindromic sequence is any type of sequence that will be same as upstream or downstream, but recognition sequence is a sequence which helps enzyme to recognise the restriction site, to be noted a restriction site may not always have palindromic sequence.

[@akshinbarathi8914]

@@arijitpalit2756 the recognition sequences are palindromic in natur buddy
mostly

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This Video was helpfull Sir

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Thanks sir

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It's very easy to understand us thank you for this video

[@anniebabu9993]

Video is really awesome but the cutting patterns of type two restriction enzymes isnot explained kindly do check

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[@anniebabu9993]

ok bro ill check that

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[@Muhammadtalhabutt94]

sir...why type 1 enzyme cut DNA upstream and down stream other than recognition site

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Sir Please Tell About SNPs And RFLP

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more concept clear...thnku sr

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Sir Please exonuclease ke bare me bhi explain kijiye...

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Tx byt please make a video of the dna modifying enzyme kinase

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[@swarnasayana8230]

can you tell me what is frequency of recognition sites.

[@marianachartian]

swarna sayana mostly one recognition site is the best for cloning or else the cloning process is much more complicated...Good day

[@architabhattacharya9690]

1/4^n ..it's the formula for finding frequencies..n is the number of bases in the recognition sequence..for example, if some Restriction enzyme cuts a 4 base sequence, then the frequency of the sequence/site will be 1/4^4 .

[@oleksandraskorobohatko2125]

It is now 5 types of restriction enzymes, update please

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Thank you

[@zayanali2881]

Sir! will you please tell me that palindromic sequence and recognition sequence is same thing or different.

[@munibabid5216]

No... these are different terms