Super glad to have found this! I did a ton of work with recombinant DNA a few years ago (since then have been on a materials science/design engineering journey) and I was looking to refresh myself on the concepts I was immersed in not too long ago. This was perfect and should honestly be shown to every fresh student in a synbio lab.
5:40 fyi You can also use the white-blue verification to see if the recombinant Plasmid is working. Here the MCS, the part where the DNA is inserted, lies within the promotor region of a LacZ gen, that will turn the colony blue, if the medium they grow on contains GalX. If the insertion was successful, LacZ cannot be produced, which means the E.Coli colony remains white. Sorting then, is simply scraping the white colony of and to proceed cloning with that. (Works only after you already pre-selected via Antibiotics) This is a great Video!
Super spannend, das ist gerade eins meiner hauptaufgaben im Labor, da ich darüber Bachelorarbeit schreibe! Wir nutzen allerdings InFusion, wofür wir keine Restriktionsseiten brauchen
If a gene is inserted incorrectly in the plasmid, will RNA POLYMERASE start transcription or not? The bacterial promoter works but rna polymerase will start transcription?
Super glad to have found this! I did a ton of work with recombinant DNA a few years ago (since then have been on a materials science/design engineering journey) and I was looking to refresh myself on the concepts I was immersed in not too long ago. This was perfect and should honestly be shown to every fresh student in a synbio lab.
5:40 fyi You can also use the white-blue verification to see if the recombinant Plasmid is working. Here the MCS, the part where the DNA is inserted, lies within the promotor region of a LacZ gen, that will turn the colony blue, if the medium they grow on contains GalX. If the insertion was successful, LacZ cannot be produced, which means the E.Coli colony remains white. Sorting then, is simply scraping the white colony of and to proceed cloning with that. (Works only after you already pre-selected via Antibiotics)
This is a great Video!
blud I DIDNT UNDERSTAND ANYTHING MY TEACHER SAID AND YET A 6 MINUTE VIDEO EXPLAINED EVERYTHING, THANK YOU
This was able to summarise Gene Cloning better than anything I've read/seen before. Thank you!
Exactly. In my food microbiology class we're currently learning about precision fermentation and this video just summarize the entire 40 slides.
Glad this popped up just when my online students read about molecular biology. Thanks, Henrik!
Would be glad if you share it among the students :D
Thank you so much for all you do. Don't ever stop posting you are helping a lot of students.
Found this as I was reviewing for my Environmental microbiology midterm thank you!!!
Super spannend, das ist gerade eins meiner hauptaufgaben im Labor, da ich darüber Bachelorarbeit schreibe! Wir nutzen allerdings InFusion, wofür wir keine Restriktionsseiten brauchen
Sehr cool!
Danke für dein Video 🙏🏽 Super erklärt und veranschaulicht. Like und Follow mehr als verdient !
Fantastic clarification!! thank you for your help!!
Great work
Brilliant!
If a gene is inserted incorrectly in the plasmid, will RNA POLYMERASE start transcription or not? The bacterial promoter works but rna polymerase will start transcription?
WOW It's not easy to explain this in 6 minutes.
Can you believe it tho
This is being taught to a grade 7
i have a paper due tmr morning. i have NOT started. i will fail.
Hindi video
"Promosm"
Thank you. Good explanation