Liquid Mushroom Culture from Gourmet Oyster Mushrooms, New Episode in How to grow Mushrooms Series
Вставка
- Опубліковано 8 чер 2024
- All the details you need to know on how to make a liquid mushroom culture of a gourmet oyster mushroom, although this technique can be used for 100's of types of mushrooms.
Liquid culture consists of a sterilized nutritious solution that has been inoculated with fungus spores or mycelium.
Andrew goes over each step from sanitization and to shows you exactly how to take a sample directly from the mushroom, put it into the liquid solution and how to care for the culture as it grows.
You can find all of our liquid cultures available here,
www.mossycreekmushrooms.com/l...
If you are seeking Mossy Creek Mushrooms, they are here!
King Blue Oyster
www.mossycreekmushrooms.com/p...
The Mossy Creek Mushrooms Pathfinder Liquid Culture
www.mossycreekmushrooms.com/p...
Golden Enoki
www.mossycreekmushrooms.com/p...
Blue Oyster Liquid Cultures
www.mossycreekmushrooms.com/p...
Chestnut Liquid Cultures
www.mossycreekmushrooms.com/p...
Golden Oyster
www.mossycreekmushrooms.com/p...
King Oyster
www.mossycreekmushrooms.com/p...
Comb Tooth
www.mossycreekmushrooms.com/p...
Don't forget to leave a message and tell Ben he's great!
Hey Moss Backs, when you buy from these companies it helps to support our channel. Please use the code "Mossy" for discounts or free stuff on your orders!
House of Hydro: Free sets of replacement discs!
www.thehouseofhydro.com/index...
Terrestrial Fungi: 10% off your order
www.terrestrialfungi.com/
Mycelium Emporium: 10% off your order
www.themyceliumemporium.com/
Shroom Supply: 10% off your order
www.shroomsupply.com/
Patreon: This is easily the best way to help support our channel!
/ mcmushrooms
Instagram:
/ mossycreekmushrooms
Facebook:
/ mossycreekmushrooms
Twitter:
/ mcmushrooms
Our website:
www.mossycreekmushrooms.com/
Check out our latest blog post:
www.mossycreekmushrooms.com/blog/
Interested in learning to grow mushrooms? Check out our consultations here:
www.mossycreekmushrooms.com/ph...
#liquid #culture #mushrooms #keepspawningculture
![[How to Grow Mushrooms] Making a Grain Spawn Master using liquid culture and agar.](http://i.ytimg.com/vi/njEi_BPBgHs/mqdefault.jpg)
The needle biopsy move is a good idea. Never thought of using the needle tip for a quick and easy way to get a piece of tissue
I came here to say this too! :)
Those are the biggest oysters I've ever seen. Well done!
I've seen so many videos and nobody has shown this technique yet! Love it.
Wow, mind is blown! Can’t wait to try this out! Thank you so much from the bottom of my heart lol
Great video your content is consistently top notch. Please keep up the good work. And thanks for sharing.
I finally feel like i know enough to be able to do the full circle from start to finish to restart again..
Thanks, subbed and will check out some more videos.
This is absolutely fascinating! I’m just getting started with mushroom growing, and the more I learn, the more interesting this becomes.
@Sarah Delaney no, that’s not sketchy at all 🙄
thanks so much for sharing this beautiful knowledge about mushroom. I've always desired to start cultivation of Oysters. this is a great eye opener 👍👍👍
Thanks for the video MCM. We use needle biopsies for most tissues. The mushroom is dry compared to human tissue but anything that is soft can be biopsied pretty easy.
You're the man!!! I had no idea I could do a tissue biopsy
Thanks for the comment about stirring releasing Co2 and allowing O2 in. The fish tank analogy helped. I have some liquid culture growing and I was stirring twice a day, I just put it on the stirrer and turned it on, and will leave it there.
Love you Andrew!! This vid been on my to watch list for a couple days. Great one
Thanks!
In labs liquid media is the preferred method for culturing, simple Erlenmeyer flasks are stoppered with Cotton wool plugs and then wrapped in tinfoil to make sure the wool does not get wet in the autoclave. Color changing Autoclave tape is stuck to the bottle to indicate the bottles that got sterilized. You culture a generation one or more master mediums and then fill the flasks from these. You can also inoculate from a petri dish, mushroom or another liquid culture by first sterilizing an inoculation loop in a flame and then transferring your subject (save on syringes). For agitation we use a lab shaker vs introducing a stirrer mainly as you can shake multiple flasks continuously while incubating them. Consider a flask and cotton wool to save $ and an inoculating loop over syringes, unless your selling them. Also consider experimenting with say a 2-4% Glucose solution for media. If you really want to get crazy look at methods like pulsed electric field sterilization of bulk substratre or growing mushrooms continually in liquid on raft scaffolds like hydroponics but for mushrooms - fungiponics :D Looking forward to your next video.
True. For the Stock Culture we use a slant agar- slower growth, lasts longer.
Then transfer to petri dish agar as Mother Culture (using inoculating loops).
Then from petri dish to liquid broth in hundreds of flasks on shakers (using inoculating pipes/borer-don't remember the exact term). Lesser liquid in larger volume flasks results in much higher growth speed but means more flasks and shakers needed.
For really large scale we used Fermenters instead of shakers.
I think we never used a single syringe for production. We use glass or metals apparatuses that can be autoclaved and reused indefinitely. Single use stuffs are for the hobbyists not professional production.
Just sharing..
Great insights! Thanks for sharing them! I'd love to explore 'fungiponics'! Would you have any links with more information about this method?
Do you have links to fungiponics ?
So you are saying, I can have unlimited liquid culture by simply using ready liquid culture to expand into diff jars?
@@EarsTwoHear yep totally, just gotta make sure to keep everything sterile
Thanks man. I used this technique for the first time with a still air box. 1 mushroom to 3 different jars. All 3 jars cleanly colonized :)
Thanks for sharing; this is a very helpful lecture
Awesome technique. Beautiful. Can't wait to try it. I'm still relatively new to growing but recently I just used clean tweezers to pull some mycelium from a block and put it in lc. Totally worked. I'm stoked.
WOW...I LOVE THE STOREROOM...AMAZING
impeccable timing my good sir!
Awesome technique. I need that book.
A wonderfull technic ... i ve learned from you about 1 year ago.. and i ve now many jars like yours and selling them .. Thank you my friend ✌
Thanks for informative video! I will be trying this.
Good teaching, thank you
Awesome video. Thanks for sharing.
Thats really cool I always go scalpel to agar to lc but I really like this for cloning. Gonna go for it! Thanks
Good methods promote growth, great video!
Excellent technique! I'm considering doing this with morels. Im going to try to go from a jar to a 5gal bucket. Then dump the bucket over a prepped burn site in the fall.
Great videos, very didactic. Thanks a lot.
Michel from Belgium, in Europe
Thank you so much! I learned a lot of useful things for myself!
i will definitely try this, Thank you
Just saying hi from Mumbai , kindly keep those videos coming
He's really an isopropyl addict. Great video of a stellar technique.
Aseptic processing OCD, it sucks to have loss from contamination, so you got to be diligent.
Making liquid culture tonight--inoculating it tomorrow! Thanks Mossy Creek Mushrooms. 😊Jen
Wow. I have always gone fruit to agar to second agar to liquid culture. Good to see this is possible. I will try direct to LC. I have some LC jars cooling right now. It is my first time using the grey leak proof lids. So far, so good.
yiur vidoes are so clean and leaborate
Dude , you are the only one I see that teaches how to cultivate mushroom. Thank you
GOURMET mushrooms ;)
Wow this is amazing
Nice Breakdown
So cool. Thanks for your guide :)
Thank you for putting this out there it really helps everyone
Hello Mossy Creek mushrooms. I went to a location yesterday that was supposed to be monster mushroom company had to drive for a little while to get there I got there and the people were rude to me they did not have the thing that I wanted they did have some sterilized grain and some sterilized substrate so I bought a bag of sterilized substrate for $30 got home with it and come to find out there is a hole in it and where they tried to seal it there is a hole big enough for me to stick four fingers and and it goes straight down to the substrate so I did not get a bag of substrate that was sterilized I got a bag that was unsterilized really frustrated at this point and probably am going to be looking at what you guys have for sale.
:( Man, that sounds like a bad day. I hope your able to get what you need and well, it doesn't seem hard to beat the competition uh?
Cute puppies! Great info! Keep rocking!
Thank you! Will do!
Great video
I love your channel i learn so much, i always thought LC was stirred to disperse the mycelium, sorta like how shaking grains at partial colonization to spread the mycelium better.
Thanks dude, I totally learned a new technique from you today! Thanks for sharing. I also purchased some liquid culture pre-mix (and used your code) from shroomsupply. I will give LC another go because my first try using Karo corn syrup was great at growing mycelium and mold no matter how clean my process was using a SAB. I also noticed when using your flow hood you should elevate your work surface just a bit so its not right at the very bottom of the flow hood. Anyway great vid, can't wait to try this out.
You guys are awesome. I'm just getting into mycology and want to work for a mushroom company until I can start my own company. Valuable info.
Thank you for the info.
good explanation bro
I love this idea..
thanks for sharing
Thanks man! great video
Best vid on the net!
Big fan of your videos sir 👌👌👌👌👌 all my liquid culture is totally successful only because of u sir…
Fantastic videos! Thanks so much for all the effort and time sharing this valuable information.
What do you inoculate your Masters Mix bags with? Grain? or directly from the liquid culture? Liquid to grain to Masters Mix?
Thanks so much and please keep up the great videos!
Great video Bro, thanks for the info
Very impressive!!! Well done!
I learn so much from YT videos. I now have another channel to add.
Great video mayne 🤗
This is amazing, thank you!
Thank you Mossy Creek for all your videos. There are many channels out there but I love your videos. As a hobbyist I find you sooooo helpfull. I've been trying Lion's Mane without success. With liauid culture I hope it works this time. Any tips wouls be welcome. Happy myco!
Awesome video!
Awesome! thank you for sharing.
Great video.
First time I've heard someone say alchyhol other than me. Big love
Hahaha That's great!
I appreciate sharing your secrets.
Glad you like them!
nobody is talking about how cute hes and he got a lovely smile
I use a technique to clone mushrooms directly in the forest. I take some sterilized butyl cap vials filled with liquid medium and use only a needle with a cilinder inside, without the syringue, to push out the piece of mushroom tissue. From now I'll use this technique, as only needs the liquid in the vial to push out the tissue.
One of the best! Thanks mate
Glad you like it!
Awesome, engaging video
thx dude! greetings form poland!
Tunes.... Love it
Thanks!
Thank you!
Great! I get more exp watching this. Thanks big man👍
Glad to hear it!
Thanks for the video brother!
NP! Thanks for stopping by!
Great video- I wish I could subscribe twice.
I haven't seen that much isopropyl since 2019... how did you clean everything this year?
hahah for real, i wanted to clean my computer back in july or so, and had to get alcohol swabs because the entire alcohol shelf was barren at every store... even the witch hazel was always gone!!
luckily it's back on the shelves now and i grabbed a full gallon, since there was a 1-per-shopper limit xD
I still used alcohol. We bought from the local distilleries that converted over to making hand sanitizer.
Love that room you have! I wish
its all in the Twist '''''!!!!
thanks a lot !!
It was an epic match to the end ☠️
Great video 📹 👍
Glad you enjoyed it
Genius! Thanks for the idea. I guess there is no need for agar plates now :D
Thank you. Thiis is what I have been looking for.
I get my from an online store, they got shroom, psychedelic and microdosing products well refined, also ships to anywhere.
He's on Instagram with the below handle..
Mycopete.
First I need that beard friend! This is cool I love earth
Thankyou❤
muchas gracias por compartir tu conocimiento :D saludos desde Chile! (L)
Hey man thanks for the useful info! Do you only do one needle biopsy per jar of LC?
That's so cool. Not messing around with spores and just using fresh shrooms. I've read that 0.3% hydrogen peroxide kills contaminations and spores but allows to grow the mycelium. However I have found no videos yet.
This man's iso game is strong!!
thank you for the video. love your Mjolnir brother ;)
My big beard brother, you are genius 😎 like
Thank you sir
Terimakasih info nya semoga sukses selalu salam dari Mr Edy Indonesia
Great content - I like the idea of the needle biopsies better than going from tissue to agar and then liquid culture. Less opportunity for contamination by exposure. Question: What is the best needle gage to collect a proper sample? Also, at what temperature do you store your liquid cultures while they propagate? I am using 1/64th teaspoon of peptone, 1/64th teaspoon of light malt extract, and 1/2 tablespoon of corn syrup with 600 ml of hot distilled water. What is your favorite recipe?
How long should you stir for? Also once the culture is established, how long will it last on the shelf? How do you store it after the culture is established?
Hi Andrew. First, thank you for your YT channel. I'm new to home growing of mushrooms and have found your videos very helpful and full of great information! Secondly, I will be using your discount over at Shroom Supply, so thank you for that as well! My question regarding liquid culture is this: how long should I place my culture jars on the magnetic stir plate each day? I'm starting working with only two types of gourmet mushrooms (oyster and lion's mane), and I've already ordered culture syringes for both types, but once the rest of my supplies arrive and I inoculate the prepped/new culture jars, what length of time will I need to let each jar sit on the stir plate? Thank you in advance for any advice/guidance. And Happy Thanksgiving to you and the entire Mossy Creek team! Peace & Respect.
Healthcare best practice for stetile technique is to wipe in a reverse spiral ... that is to start in the middle and spiral outward. I think it would be best to end by coming off of the table away from the fan.
Might be overkill but its evidence based.
Really apreciate your content guys...so usefull!! Just one doubt...does the spraying bottle has 70 grade alcohol inside?? Is it the same you have in the other container you use for wiping down the table?
Awesome!
Thanks!
Loved your videos you've helped me so much. My question is how long do the cultures last after doing the tissue biopsy? I oh ant to clone a few mushrooms I like but I don't plan on using the cultures right away on grain.
Very cool.
Thank you!
I came up with the same process on my own, just searched to see if anyone else was using it--pretty cool. mine method is slightly different but essentially the same.
Bro, you use iso like I use iso. I like it!!!!