just got out of COVID quarantine and my prof didn't explain this at all and the figures were making it really confusing. I skipped ahead to the part I was confused and it took literally a minute and I completely understood this topic. thank you so much for this video that you created like 5 years ago
I had a hard time trying to understand this simple concept and finally it all made sense. Bless your soul for making such content, you are helping so many people including me to understand basic fundamentals in biochem/biology/chem.
Thank you, Sir. Your videos are really helpful for me to understand the concepts of biochemistry. And I'd strongly recommend to anyone to watch your videos to clear their doubts.
why is the charge of carboxylic acid at pH 7 is -1? why not -1 minus 1? because initially it has a negative 1 charge then after deprotonation you'll subtract 1?
Damn! You have no idea how long have been going through videos on UA-cam and still couldn't answer a question on this topic ,I'm preparing for MCAT, this will go a long way. Only way to pay you is to share the video
For Glycine when pH is 12 how does the net charge is -1? In N-H because of the deprotonation the charge is -1 and in COO- bond there is -1 charge so the total net charge is supposed to be -2. When pH=7 you are counting the charge of COO- is -1 to get the total net charge. Then why when pH=12 you are not counting the charge of COO- group -1 charge to get the total net charge? Can you please explain it?
is net charge would be -2 at pH 12 since there is already -1 (pH 12 does not change -1 and -1 stays -1) in carbonyl group and very basic condition(pH 12) rips out H from NH which makes -1 as you mentioned, So -1 + -1 =-2 isn`t it?
The pI of glycine is written as 5.97 in books. Is that correct if we take ph 5.97 instead of 7 in this calculation? In books the formula for pI is , 1/2 (pK1+ pK2) i.e., 1/2 (2.34+ 9.6) = 5.97
Are you sure that the pI for glycine is 7 and not 5.97...🤔🤔 Isn't pI supposed to be calculated as (pKa1+pKa2)/2 For glycine it would be (2.34+9.60)/2=5.97
Good afternoon. How many moles of NaOH per mole of histidine should be added to increase 6.5 to 9 pk are given. What will be the charge of histidine at the given pH values, how will it move during electrophoresis? Thanks in advance for your answer.pk1 = ¸3.1, PK2 = 6, PK3 = 8
pls make a video to calculate the net charge and PI (isoelectric point) of a polypeptide at different pH. Problem N0.- 11 of Lehninger biochemistry, chapter -3, 4th edition
sir what will be the charge of glycine at pH 4,5,6,7,8, and 9. according to you will be zero.then what is PI value for glycine. it seems all of these ph values will be PI for glycine as well.
Sir, I'm a JEE aspirant and I recently faced a problem on Biomolecules The question had three phases of glycine where [A]- Both acid part and -NH part was protonated [B]- Only the -NH part was protonated [C]- Both the parts was deprotonated pKa1=2.3 pKa2=9.7 The question asked if at pH=2.3, half of [A] is converted to [B] or not? So sir, can I consider it to be correct that yes it is converted, on the pretext that half part is protonated and half is deprotonated? (I am having confusion in the part where it asked 'converted', and I am not sure that half protonation would result in half conversion of phase [A] to phase [B])
His calm demeanor, how he talks, and his accent are some of the qualities of this man. Thank you, sir!
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this man is a king, explained so concisely what my professor could not explain in two hours... thank you sir!
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just got out of COVID quarantine and my prof didn't explain this at all and the figures were making it really confusing. I skipped ahead to the part I was confused and it took literally a minute and I completely understood this topic. thank you so much for this video that you created like 5 years ago
Can you explain why did you put carboxyl group in ionized state, whereas amino group was not ionized.(in cell conditions both are ionized)
Thankyou for simplifying this to me I definitely feel more confident for my exam.
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Shomu Sir , i like your personality and also the way of talking. wow
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I had a hard time trying to understand this simple concept and finally it all made sense. Bless your soul for making such content, you are helping so many people including me to understand basic fundamentals in biochem/biology/chem.
Wow UA-cam has the best teachers!
Thank you
Thanks a lot Sir,,,,you made it crystal clear....The concept about that become as clean as fresh water.... 🙏🙏🙏Keep going sir....
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Excellent sir
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You made this so simple, thank you.
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Your explanation is too much good sir ♥
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you've enlightened my understanding better. Thank you very much SIr
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Eventhough there was a mistake,but thanks because you made me sure about my idea! I concluded the same thing when I studied amino acids alone!
Excellent explanation
Thank you
@@shomusbiologyofficial welcome
Thank you for giving me some clarity on this topic, I am currently taking a self-taught biochemistry class with a very confusing professor. Thank you.
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Thank you, Sir. Your videos are really helpful for me to understand the concepts of biochemistry. And I'd strongly recommend to anyone to watch your videos to clear their doubts.
Glad to hear that you are getting benefit from the videos
Ok
Thisari Upamalika ok
You are doing great job sir.. God bless you
+Anjali Shakya thank you. Glad you liked my lectures
Anjali km yes
Hi
why is the charge of carboxylic acid at pH 7 is -1? why not -1 minus 1? because initially it has a negative 1 charge then after deprotonation you'll subtract 1?
Sir me apke video se hi hamara pura syllabus ache se samaj me aa jata he so tnx and vert good work and very helpful for our study
Thanks for the video.. cleared the concept ❤️❤️👍👍
Thank you is a very small word for your videos sir.. You are great
Thankyou sir for making the concept easy to understand 🙏
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You are the best! I never understood this topic so clearly before. Same for many other topics you taught in your other videos. Thank you so much!
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thank you very much sir you're all vidio help me lot
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Damn! You have no idea how long have been going through videos on UA-cam and still couldn't answer a question on this topic ,I'm preparing for MCAT, this will go a long way. Only way to pay you is to share the video
Thank you so much for appreciating my efforts
Thank you sir 👍👍....
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Your videos helped me lot in class 12
You're the best💯
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Great sir🤗
Thank you
will de-protonation of amine happen at pH 10 for developing a negative charge over -NH gr.
He taught the subject better than my professor
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For Glycine when pH is 12 how does the net charge is -1? In N-H because of the deprotonation the charge is -1 and in COO- bond there is -1 charge so the total net charge is supposed to be -2.
When pH=7 you are counting the charge of COO- is -1 to get the total net charge. Then why when pH=12 you are not counting the charge of COO- group -1 charge to get the total net charge? Can you please explain it?
Sir I have some confusion with glycine formula.
In the video it is NH - CH2- COO-.
Is it correct.
Yrs
Thank you so much!! It's so clear to me now!!
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Thank you sooo much sir .....
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Omg Thank you so much sir! you explained it very well
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sir can u make video on longpolypeptide chain like this. plzz soon becoz it gives idea how can calculate
bro ur the best
Thank you
is net charge would be -2 at pH 12 since there is already -1 (pH 12 does not change -1 and -1 stays -1) in carbonyl group and very basic condition(pH 12) rips out H from NH which makes -1 as you mentioned, So -1 + -1 =-2 isn`t it?
I agree with you ,we need clear this point
Legend! Thank you so much.
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There should be a Positive Charge over NH . If we consider that, all the answers change. But concept explanation is spot on
No. The charge depends on pH
@@shomusbiologyofficial Oh Alright... thanks
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Awesome lacture... 👌👌Thank you so much for clearing my concepts with amino acid charge calculation 🙏🙏😊
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@@shomusbiologyofficial multipel ?
veryveryveryveryveryveryveryveryvery dificult
Thanks you so much!! Where can I see the second part of the class? I need to know how to calculate the net charge for a polypeptide
Very informative sir.
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When I get confused in some topic in biology ,I usually watch your videos, it gives clarity.tq sir
Thank you so much for this very simple and informative lecture
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let me buy a beer for that man
Let it be
thank you sooooo much man
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Thank you very much for this video it really helped with my studying!!
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Oh! what an explanation
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awesome thanks. God bless
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nice clarity
Thank you
sir how to find the next class?
Thanks for the Video. Please check your calculations before uploading...
thank you
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When UA-cam teaches you more than a top University :/
great job!
Thank you so much sir i completely understand this topic🥰😅
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Nice🔥🔥🔥
god bless your soul
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Thank you soooooooo much Sir!
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Sir could you plz elaborate how the molecule are protonated and deprotonated .
please make a detailed video on thermodynamics of protein stability and vant hoffs equation
+20tejas okay. Will try to do that
Shomu's Biology thank you.☺
Plse sir
thank you for this video friend
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very informative
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sir I am a student of Physiology(b.sc)...IIT jam ka kaise preparation lena chahiye..please inform me.....
Your videos are really helpful...I m totally dependent on ur videos. Thanks a lot...you are doing a great work
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Shomu's Biology : No concept is difficult with your way of teaching.
thanks
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sir please solve the analytical part of csir net 2017.. your videos help a lot.. thank you so much sir.
+Shonali Krishnani I will
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In case of titration of a peptide chain, should we start from the fully protonated form of the peptide or the fully deprotonated form?
thanks man, Greetings from Germany :D
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where can i find part 2? it's not shown to me ,if someone could put its link i'd be thakfull
thnks pro
+Biologie facile you're welcome
Thank you souch sir
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Nice video
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The pI of glycine is written as 5.97 in books. Is that correct if we take ph 5.97 instead of 7 in this calculation? In books the formula for pI is , 1/2 (pK1+ pK2) i.e., 1/2 (2.34+ 9.6) = 5.97
You saved meeeeeeee thank youuuuu watching this video will help a lot of embaressed biochem students because of their stupid professor
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Where I can find the second video for polypeptide?
Sir pls send me link of next video in wich we can calculate pI
Okay
@@shomusbiologyofficial Where is it?
Sir pls make a video on how to calculate the absorbance of an amino acid ..
Okay
Are you sure that the pI for glycine is 7 and not 5.97...🤔🤔
Isn't pI supposed to be calculated as (pKa1+pKa2)/2
For glycine it would be
(2.34+9.60)/2=5.97
exactly as we will get zwitterion in between these two ph's so he is wrong on this part
Can someone put in the link for next lecture?
Where can I find the second video
Where is ur next class regarding this topic.
excellent
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Good afternoon. How many moles of NaOH per mole of histidine should be added to increase 6.5 to 9 pk are given. What will be the charge of histidine at the given pH values, how will it move during electrophoresis? Thanks in advance for your answer.pk1 = ¸3.1, PK2 = 6, PK3 = 8
Sir pls make vdo on optical properties of aminoacids
How to calculate the charges on acidic Amino acid and basic Amino acid at different ph
Sir ionizable R grp wale..ka video hai?
pls make a video to calculate the net charge and PI (isoelectric point) of a polypeptide at different pH. Problem N0.- 11 of Lehninger biochemistry, chapter -3, 4th edition
Hello sir, i have a question whether the Pka value would be given in CSIR-net question or we have to memorize??
Sir can't understnd when pH=pKa..otherwise wonderful video
sir what will be the charge of glycine at pH 4,5,6,7,8, and 9. according to you will be zero.then what is PI value for glycine. it seems all of these ph values will be PI for glycine as well.
Please send me the link for next video sir of charge of polypeptide
Sir, I'm a JEE aspirant and I recently faced a problem on Biomolecules
The question had three phases of glycine where
[A]- Both acid part and -NH part was protonated
[B]- Only the -NH part was protonated
[C]- Both the parts was deprotonated
pKa1=2.3 pKa2=9.7
The question asked if at pH=2.3, half of [A] is converted to [B] or not?
So sir, can I consider it to be correct that yes it is converted, on the pretext that half part is protonated and half is deprotonated?
(I am having confusion in the part where it asked 'converted', and I am not sure that half protonation would result in half conversion of phase [A] to phase [B])
Sir, it means for glycine pI ranges from ~3 to 9 ?