Watching these videos with your notes is honestly best revision technique. I ran through topic 5,6,7,8 all in 3 days. Good luck for bio p2 tomorrow everyone .
you are a GOATED teacher, literally helped me understand and be able to apply the biology I learn from videos to answering questions. Thank you. I've been telling all my friends about your content and it's helped them tremendously.
non glowing colonies contain recombinant plasmid... but doesnt that ignore the fact that the plasmid may not have been taken up at all by the host cell, hence why its not glowing? (7:30 for point of reference)
This is what my teacher said - ‘with fluorescent markers you would need two markers to avoid this problem. You would probably need a fluorescent marker which is not disrupted and to insert the gene of interest elsewhere in the plasmid. You would also need to include another marker to prove the plasmid has been taken up. This could be an antibiotic resistance gene. Essentially, fluorescent markers are not used really on their own.’
@@MissEstruchBiology Hi Miss, Thank you for reply. Would it be possible to do a video on statistical tests and maybe go through past paper questions which include error bars?
Hello, absolutely, Statistical analysis is on the list of videos to try to complete within the next month :) ! I plan to do some worked examples on my Instagram IGTV before that though.
For the AQA specification, is it necessary to know the exact process and names of the antibiotics used in antibiotic-resistance marker genes? I've seen on other resources for this specific marker gene that says that the target cells with the antibiotic-resistance marker gene will survive when antibiotics are applied to them whereas the other target cells will be destroyed, therefore meaning that the cells with antibiotic resistance must also be expressing the target gene in their plasmid DNA.
Bit confused on antibiotic-resistance marker genes part, I’m confused on the actual physical process with the sterile velvet block, what do you physically do with the block and the agar plate with the colonies on?
Thank you so much Miss Estruch!! I want to ask u a question regarding to identifying the bacteria/cells which carry recombinant plasmid or not. Is the last method 'gene coding for enzyme' blue-white screening? Last but not least, I hope u can stay in the pink of health at this very moment!
Hello, You're welcome! Glad it helped. It may be called blue-white screening by some, as these are two options of colours you would observe for a positive/negative transformation result . Thank you, keep well and safe too!
Really pleased to hear they are helping you! Good luck with your exams! Currently, on the topic 8 playlist there is: Mutations Stem cells Epigenetics Cancer and tumours creating DNA fragments (In vivo) In vivo cloning Use of markers (this video) Still to make are: Transcriptional factors regulation of translation genetic fingerprinting PCR I am working hard on completing the full A-level course ASAP, but I am on a race against the clock of giving birth to my twins who are due late September/early October. Fingers crossed I get most/all up before the exams.
Hello, I just had a question that on the spec for AQA it says that the students will not be required to recall any specific marker genes so does that mean that we don't need to know any of the markers like fluorescent markers?
why does my textbook say that you discount the ones which do not turn the colourless substance blue with the lactase as a marker? I thought the ones which do not turn the colourless substance blue would be the plasmids of interest as these are the ones that have taken up that dna fragment?
is this the kerboodle textbook?? i noticed that as well, asked my teacher and she just said it was a typo so the sentence should be 'where the gene has not been taken up by the bacteria, they WILL turn the substrate blue. these bacteria can be discounted.' i've noticed a few typos in the online book idk if it's the same with the physical copy but it definitely isn't meant to say 'will not turn the substrate blue'
Watching these videos with your notes is honestly best revision technique. I ran through topic 5,6,7,8 all in 3 days. Good luck for bio p2 tomorrow everyone .
how were you able to get through so many topics in a day? How many hours were you doing haha
@@h4rryjeim being serious from morning till night just grind, that’s what im doing taking breaks ofc, i got through all the paper 2 topics in 4 days
Thank you so much. You are saving my A-levels :)
Ahh so pleased the videos are helping you so much!!! 😊
you are a GOATED teacher, literally helped me understand and be able to apply the biology I learn from videos to answering questions. Thank you. I've been telling all my friends about your content and it's helped them tremendously.
awww you're too kind! Thank you so much😁😁😁 So glad the videos are helpful
So helpful, thank you!
Ah thank you 😊 Really pleased it was useful
your videos are so helpful !!
I'm so glad! Thank you
non glowing colonies contain recombinant plasmid... but doesnt that ignore the fact that the plasmid may not have been taken up at all by the host cell, hence why its not glowing? (7:30 for point of reference)
I thought this aswell- any ideas?
@@katjolly9514Are you still stuck on this? I've asked my teacher so can let you know what she says :)
This is what my teacher said - ‘with fluorescent markers you would need two markers to avoid this problem. You would probably need a fluorescent marker which is not disrupted and to insert the gene of interest elsewhere in the plasmid. You would also need to include another marker to prove the plasmid has been taken up. This could be an antibiotic resistance gene. Essentially, fluorescent markers are not used really on their own.’
Best explanation....😁😁😁
Thank you. This is so helpful, can you please make videos on the remaining sections of recombinant DNA please.
all of recombinant DNA videos are in the topic 8 playlist, the only video missing from all of topic 8 is genetic fingerprinting.
Hope this helps.
Thank you so much for this video!! I finally get this :))
Hi Hivda,
I'm so glad the video helped you!
Let me know if you have any questions of request for other videos.
@@MissEstruchBiology Hi Miss,
Thank you for reply. Would it be possible to do a video on statistical tests and maybe go through past paper questions which include error bars?
Hello, absolutely,
Statistical analysis is on the list of videos to try to complete within the next month :) !
I plan to do some worked examples on my Instagram IGTV before that though.
Miss Estruch Hey Miss,
That’s perfect, thank you so much! I’ve also follow you on Instagram :))
@@user-my6lv1ur3p Great :D I hope you like the quizzes on there
For the AQA specification, is it necessary to know the exact process and names of the antibiotics used in antibiotic-resistance marker genes? I've seen on other resources for this specific marker gene that says that the target cells with the antibiotic-resistance marker gene will survive when antibiotics are applied to them whereas the other target cells will be destroyed, therefore meaning that the cells with antibiotic resistance must also be expressing the target gene in their plasmid DNA.
The aqa textbook places the marker gene on one side, saying transformed cells will fluoresce under UV light?
Bit confused on antibiotic-resistance marker genes part, I’m confused on the actual physical process with the sterile velvet block, what do you physically do with the block and the agar plate with the colonies on?
You press the velvet block onto the original plate of bacteria and the press it onto the next agar plate. This transfers over some bacteria. 😊
@@MissEstruchBiology ahh thank you, helps a lot
Thank you so much Miss Estruch!!
I want to ask u a question regarding to identifying the bacteria/cells which carry recombinant plasmid or not.
Is the last method 'gene coding for enzyme' blue-white screening?
Last but not least, I hope u can stay in the pink of health at this very moment!
Hello,
You're welcome! Glad it helped.
It may be called blue-white screening by some, as these are two options of colours you would observe for a positive/negative transformation result .
Thank you, keep well and safe too!
You’re videos have literally saved my autumn exams😂
But are u going to make a vid on in vitro cloning and genome projects?
Really pleased to hear they are helping you! Good luck with your exams!
Currently, on the topic 8 playlist there is:
Mutations
Stem cells
Epigenetics
Cancer and tumours
creating DNA fragments (In vivo)
In vivo cloning
Use of markers (this video)
Still to make are:
Transcriptional factors
regulation of translation
genetic fingerprinting
PCR
I am working hard on completing the full A-level course ASAP, but I am on a race against the clock of giving birth to my twins who are due late September/early October.
Fingers crossed I get most/all up before the exams.
Hello, I just had a question that on the spec for AQA it says that the students will not be required to recall any specific marker genes so does that mean that we don't need to know any of the markers like fluorescent markers?
yeah, you just need to understand how they can identify the transformed cells, but no example
is it on the aqa spec that we know the names of the tetracycline gene and the amphicillin gene ?
No, it states no examples are needed.
do i need to know the names of tetracycline and ampicilin for the exam ?
no you won't need to
@@MissEstruchBiology thank you miss, your vidoes have literally been helping me so much!!
why does my textbook say that you discount the ones which do not turn the colourless substance blue with the lactase as a marker? I thought the ones which do not turn the colourless substance blue would be the plasmids of interest as these are the ones that have taken up that dna fragment?
is this the kerboodle textbook?? i noticed that as well, asked my teacher and she just said it was a typo so the sentence should be 'where the gene has not been taken up by the bacteria, they WILL turn the substrate blue. these bacteria can be discounted.' i've noticed a few typos in the online book idk if it's the same with the physical copy but it definitely isn't meant to say 'will not turn the substrate blue'
Thanks miss
you're welcome 😊
hi miss, is there any significance of inserting the DNA fragment in the middle of the marker gene only and not at the start/end?
Yes, it needs to be in the middle yo disrupt it. At the start or end wouldn't change the protein being made.😊
im confused on the fluorescent gene marker, which one contains the required gene, the glowing or the non-glowing?
Non-glowing I think
Yup, non glowing, as insering the new DNA disrupted the gene to make it glow
T-H-A-N-K-S
you're welcome 😊 Hope it helps
im cooked