This is very random but I really appreciated how calmly you spoke in this video, it really helped me concentrate and stay focused as I find this topic quite difficult 😊thank you
Hello, great video! I was just wondering, wouldn't washing away the DNA probes also wash away the hybridised DNA probe and patient DNA sample? Or is it like the ELISA test where the patients DNA sample is stuck to a surface and can't be washed away?
Hi Miss, I have a quick question as I can't get in touch with my teacher for a while, she has recently sent us this topic list for our personalised upcoming exam: 1) Cell Biology 2) Organisation (is this just how we organise things into cells, tissue, organs etc etc? Or taxonomy?) 3) Infection and Response 4) Bioenergetics 5) Homeostasis and Response 6) Inheritance, Variation and Evolution 7) Ecology 8) Practical Skills Am I right in only focussing on Topic 2, the second half of Topic 4, as well as Topics 5, 6 and 7? Completely ignoring Topics 1, 3 and 8? Looking at the topics and their content, I believe this is right, but being both an anxious and clumsy person I fear missing something out and so am not so sure. . .
Hello, I would say so, but I'm not quite sure what is meant by organisation. You're correct that it refers to cell, tissue, organ etc.. but your teacher may mean know the details of different organs and tissues, which is in topic 3. Good luck with your exam 🤞🤞
Nor exactly, a DNA fragment is a small piece of DNA. a DNA probe is a short single stranded piece of DNA that is labelled radioactively or fluorescently
@@saniyasiddeka1364 I'm pretty sure the DNA fragment is more similar to an allele (just a fragment seems to be larger). so similarly to a primer in a PCR, the probe attaches to the siDNA (single stranded) and scans along it until the desired allele is found then attaches to it, therefore allowing scans to identify the allele. DNA fragments are referred to in PCRs not DNA probes, where primers attach to the start of a DNA fragment within a siDNA (so the primer is also single stranded) allowing an enzyme to then bind called taq polymerase to then create a new strand of DNA starting from the primer if that makes sense.
Though my question would be, knowing that a primer isn't the same size as a DNA fragment as it only attaches at the start, is a DNA probe the same size as an allele? like the same number of nucleotides along? or does it not need to be the same size as long the probe is complimentary to part of the allele? thank you
This is very random but I really appreciated how calmly you spoke in this video, it really helped me concentrate and stay focused as I find this topic quite difficult 😊thank you
ahh thank you. Glad my voice made it easy to listen to
thank you so much for the whole topic 8 playlist - i feel like i finally understand!!
Glad it was helpful!
Hello, great video! I was just wondering, wouldn't washing away the DNA probes also wash away the hybridised DNA probe and patient DNA sample? Or is it like the ELISA test where the patients DNA sample is stuck to a surface and can't be washed away?
I'm guessing it's like the ELISA test
hi miss, do you have a video on gene therapy? Thank you so much
very helpful thankyou!!!
So pleased it helped you 😊
would we asked to know how you find the dna base sequence? or could i simply say find the amino acid sequence-->mrna-->dna
Hi Miss, I have a quick question as I can't get in touch with my teacher for a while, she has recently sent us this topic list for our personalised upcoming exam:
1) Cell Biology
2) Organisation (is this just how we organise things into cells, tissue, organs etc etc? Or taxonomy?)
3) Infection and Response
4) Bioenergetics
5) Homeostasis and Response
6) Inheritance, Variation and Evolution
7) Ecology
8) Practical Skills
Am I right in only focussing on Topic 2, the second half of Topic 4, as well as Topics 5, 6 and 7? Completely ignoring Topics 1, 3 and 8? Looking at the topics and their content, I believe this is right, but being both an anxious and clumsy person I fear missing something out and so am not so sure. . .
Hello,
I would say so, but I'm not quite sure what is meant by organisation. You're correct that it refers to cell, tissue, organ etc.. but your teacher may mean know the details of different organs and tissues, which is in topic 3.
Good luck with your exam 🤞🤞
Do we not use gel Electrophoresis in genetic screening?
And what is the purpose of gel Electrophoresis?
its used to separate the DNA fragments, so they can be heated to form single strands and allow DNA probes to bind
Do you need to know the Sanger sequencing method?
Not for AQA
do you have a video on DNA sequencing techniques? what is the Sanger method?
nice pfp. i can see ur filled with thoughts
so is DNA fragment and DNA probe the same thing at 4:00
Nor exactly, a DNA fragment is a small piece of DNA. a DNA probe is a short single stranded piece of DNA that is labelled radioactively or fluorescently
@@MissEstruchBiology ohh so the DNA fragment becomes a DNA probe once radioactive/fluorescent label is added
@@saniyasiddeka1364 I'm pretty sure the DNA fragment is more similar to an allele (just a fragment seems to be larger). so similarly to a primer in a PCR, the probe attaches to the siDNA (single stranded) and scans along it until the desired allele is found then attaches to it, therefore allowing scans to identify the allele. DNA fragments are referred to in PCRs not DNA probes, where primers attach to the start of a DNA fragment within a siDNA (so the primer is also single stranded) allowing an enzyme to then bind called taq polymerase to then create a new strand of DNA starting from the primer if that makes sense.
Though my question would be, knowing that a primer isn't the same size as a DNA fragment as it only attaches at the start, is a DNA probe the same size as an allele? like the same number of nucleotides along? or does it not need to be the same size as long the probe is complimentary to part of the allele? thank you
Thank you. Is this on the aqa spec?
hello,
yes, it's in topic 8.