ELISA (Enzyme-linked Immunosorbent Assay)

Hey Friends,
ELISA, short for 'Enzyme-linked Immunosorbent Assay', is a powerful technique to detect substrates (e.g. an antigen). There are multiple subtypes and variants of ELISA. Here, the most common Sandwich ELISA is explained.
More about the DIRECT ELISA:
• Direct ELISA in 1 minute
More about the INDIRECT ELISA:
• Indirect ELISA in 1 mi...
An article about the Enzyme-linked Immunosorbent Assay:
www.thermofisher.com/de/de/ho...
Cheers
Henrik
Time Code:
0:00 What is an ELISA?
2:00 Sandwich ELISA example

[@henrikslab]

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[@wodecka736]

dude, you're saving my life for tomorrow's biochem module

[@dibyajyotichattopadhyay7154]

Seriously

[@StevenMP1993]

The one video on ELISA that actually helped me understand. Thanks man!

[@artfromtheheart833]

Precise concise and accurate thanks alot

[@sarcicek6864]

I've discovered your channel yet. It is amazing for molecular biology students. 🙏🎉

[@rebeckaeriksson3731]

This is a great video! Thank you!

[@chang6362]

Very helpful video. Thank you!

[@mayaskaaa]

so easily explained. thank you so much ❤

[@yelxi]

Thank You, Sir. You made it so easy to understand.

[@mahmad3825]

Thankyou very much. You made it so simple

[@MAZINO225]

Today is my biochem University. Thank you

[@priya24612]

thia channel deserves more than the views it already has ❤🎉

[@timb7298]

thank you for this insight

[@axediepied6993]

Amazing explanation

[@isabelizseijo2781]

super easy to understand thanks

[@Isa-gy5yg]

Thank you!

[@yeukaiwadzanaingwazane3680]

Thank you very much.. your videos are helpful

[@masstorm7724]

Danke Henrick :)

[@Us33rr6]

Vielen dank!!

[@maisamelsayed]

Thank you a lot! 😁

[@SaahilMohta]

Great explanation!

[@naveenkamala9472]

Great presentation

[@mahxylim7983]

thanks! i got it in a glance

[@user-rh1dy5if9q]

Thank you❤

[@tunetonic04]

Thank you so much

[@strivingforexcellency]

Thank you

[@notme7310]

Omg thank you so much!

[@AnjorinOmotola]

Bless you ❤

[@guerroujadam8812]

man u made it so easy to understand even for a Frenchman ty so much ur video is easier to understand than my lecon xd

[@sanjaykumar-ew6mb]

❤ thanks for the nice video dear sir ❤

[@09gabriel92]

We love you man

[@albertovisconti352]

Hi, what programme do you use to make these animations?

[@gilian2587]

Beer's law -- applied to proteins; that's neat. But it looks like the assessment to determine the quantity of protein in a sample is strongly dependent on historical data; where you can compare similar samples to each other. ... ... or just read the data sheet provided by the supplier of the ELISA. After doing some reading on this... it looks like most ELISA samples are actually created by specialized laboratories that hire a lot of Peptide Chemists. Surprisingly... Peptide Chemists are paid around $45,000-75,000 per year (well; for ONE datapoint, but that ONE datapoint was requiring that the applicant had a PhD). I am a bit surprised at how saturated the Chemistry job market really is at the moment; that seems like low pay for pretty highly skilled work.

[@reet1825]

Thnk u ❤❤

[@zoedk2266]

Thankyou!

[@user-pt5iv6um6r]

Tôi thích sản phẩm này

[@Raphaelpoh]

Which type of ELISA is suitable for very small viruses such as mouse parvovirus?

[@izza4780]

Nii shoooperadaa 🙈🙈

[@DestinyCorton]

is it qualitative or quantitative?

[@markdolan914]

Good info.. from Bruno😏🤣💀 #yahhh

[@ornulusoundeffects6423]

Is this the same thing what we do in SDS page?

[@fresherm3396]

❤

[@fontexstudios]

what illustration program do you use?

[@henrikslab]

Microsoft Office Powerpoint

[@pacokolb885]

So whats the diffrence between western blot and elisa indirect variation?

[@henrikslab]

I assume you have noticed that both techniques use an antibody against the antigen and another secondary antibody which is detectable!
The difference is that western blot (WB) is able to reveal the protein size, whereas ELISA is not. Also, ELISA allows for a more accurate quantification that WB.

[@pacokolb885]

@@henrikslab thank you very much:)

[@arunmoses2197]

@@henrikslab How does ELISA allow for more accurate quantification?

[@philnevillesleftfoot]

That's my name
Dude

[@elisa174]

My name is Elisa, lol

[@user-wk7vp1lb1b]

Why would you use direct vs indirect ELISA?

[@quelquun2340]

When you want to know if there is Antibody in your solution that are specific to your Antigene

[@shreyaskulkarni5312]

Omg... That's it!!! It is made easy for me..
Supb channel on yt🔥

[@izza4780]

Hiii ELISA 🤣🤣

[@philnevillesleftfoot]

I'm Elisa come on dude

[@sonniemaluenda6153]

Elisa is name of A kid Elisa is my daughter

[@elizabethgalicia6069]

Wth why is my name a test? 💀💀
I just searched up my name
I WILL SUE YOU
(Jk I’m joking)