From now on you can become a member of this channel! You will have access to some cool emojis like this: . This is of course voluntary, but the financial support helps me a lot.
Thank you. Yours is the only really useful channel on YT for biochemical methods I could find. As final revision for my exams these videos are perfect - all the details pop into my mind thanks to you so clearly recapitulating the basics for me.
Thanks. But you should draw proteins in line shape, not in folding shape.because after SDS PAGE, all folding shapes of proteins change into line shape as we use Beta-mercaptoethanole.
Why do we need two antibodies? Is it not possible to adjust the first antibody into one that can be conjugated by the peroxidase and is complementary towards the protein?
In some cases, there are primary antibodies that are already conjugated with a detection label, but these are usually limited to a few specific types of labels, and the specificity and sensitivity of these conjugated antibodies can sometimes be lower compared to using a secondary antibody. The use of two different antibodies in western blot is to increase the specificity of protein detection. This two-step process ensures that the signal generated is specific to the target protein, rather than non-specific binding of the detection reagents. It is possible to change the first antibody into one that can hold the marker, but it is highly laborious to do so. The process of conjugating a detection label to an antibody requires specialized expertise and conditions, which can be difficult to replicate for a large number of antibodies.
This is not true! Indeed the cathode for western blot is negatively charged (-) and the anode is positively charged (+) here. It is a common confusion for students, and I remember me asking the same question in one course... (the confusion comes from the fact that cations are positively charged, right?) Best Henrik
From now on you can become a member of this channel! You will have access to some cool emojis like this: .
This is of course voluntary, but the financial support helps me a lot.
Explained this better than my 4 hour lab. Thank you
Thank you Henrik, brilliantly explained and simplified, keep them coming please
THANKS! You explaned the most important parts so well.
Thank you. Yours is the only really useful channel on YT for biochemical methods I could find. As final revision for my exams these videos are perfect - all the details pop into my mind thanks to you so clearly recapitulating the basics for me.
The animations really help a lot to understand the concept! Thank you!!
Schön visuell und super verständlich erklärt! Danke dir
IT´s brilliant how you make something looks so complicated in a very simple way i love ur vidos and ur talent
Thanks. But you should draw proteins in line shape, not in folding shape.because after SDS PAGE, all folding shapes of proteins change into line shape as we use Beta-mercaptoethanole.
Thank you for making me understand Western blot steps concise and clear.
Excellent series of videos( enjoyed SDS-PAGE, Western Blot).
Thank you Henrik!
this the best course in my life Thank you habibi!
You have explained it in an amazing way
Best science channel on youtube!
Such an excellent explanation
Excellent explanation, so glad I found this video.
Happy to hear!
short and sweet! thank u!
Thank you so much! I felt very lost in my lab prep but this makes so much sense now :)
Truly a great video!
great explanation! thank you so much, now i understand western blotting!
Wow! Amazingly explained, very easy to understand! Thank you so much for this.
Very well-explained, Thanks!!!!!
Brilliant!
You helped me understand it within 4 minutes. Thanks 👍
Henrik, ich küsse dein Herz. Du rettest mich bei meinem Biochemie-Praktikum! Danke!
Medizin?
@@ornulusoundeffects6423Ja 😁
Explained than my professor. This is legit.
This was so clear to understand! Thank you so very much!
Wow.... Hats off to your explanation🔥🔥🔥🔥
Many thanks
phenomenal explanation! thank you !
Excellent and amazingly concise explanation! Thank you!
short. simple. easy to understand. tysm
Thank you so much!
Helped so much ❤❤
Nice explanation
Great explanation! thank you
Very clear, thank you
Bro if i could give you a hug🙂
You saved me for my exams 💜
Thank you!
Brilliantly explained. Thank you 😊
thank you henriukkkk
Omg so helpful!! Thanks for making this animation!!!
Thanks very helpful
This was so helpful, thank you!!!
Very good video!
Thank you.. so much
amazing! i got all the info for my thesis. thank u
Well explained thanks!
this is so helpful thanks !
Thank you very much 🤩
Very useful
Behind helpful. Thank you.
Bro you are frkn amazing god bless you
perfect. thank you.
Thank you so much
very simple and amazing, thanks alot
Thank you ❤
thanks! now I finally get it
Abig thanks 🎉❤
Why do we need two antibodies? Is it not possible to adjust the first antibody into one that can be conjugated by the peroxidase and is complementary towards the protein?
In some cases, there are primary antibodies that are already conjugated with a detection label, but these are usually limited to a few specific types of labels, and the specificity and sensitivity of these conjugated antibodies can sometimes be lower compared to using a secondary antibody.
The use of two different antibodies in western blot is to increase the specificity of protein detection. This two-step process ensures that the signal generated is specific to the target protein, rather than non-specific binding of the detection reagents. It is possible to change the first antibody into one that can hold the marker, but it is highly laborious to do so. The process of conjugating a detection label to an antibody requires specialized expertise and conditions, which can be difficult to replicate for a large number of antibodies.
Why does one need two type of antibodies? Why can't you have the first (primary) one connect to the enzyme? You wash, add substrate, done?
great video
that was so helpful☺ thank you so much
Excelente video, gracias :)
Good work
Hatts off to you 🥹♥️
Thanks!
thank you kind sir
Thanks
Thanks a lot.
Thanks you
Amazing
thanks ,God please you
capo, gracias!
I apologize but isn't the anode negatively charged?
No dude thats anions! Anions are negatively charged particles that move towards anode which is positively charged :D
Why can't they just have an enzyme limked to the first antibody, as with the way they'd carry out direct ELISA?
I’ve had the same question.
Thank you for good vedeo. I can development
can you make a video on isoelectric foccusing please
Not planned at the moment, but I put it onto the long list of ideas!
is this the principle for luminex as well?
Great clear explanation
Very useful! thank you!
❤️❤️❤️❤️
it is a helpful video!!
Is six weeks definitive for this analysis hiv
Thnk u
You´re welcome, Kakashi
There is a little mistake The Cathode is + and the Anode is -
apart from that I gave you a Like (The video is perfectly explained) Thank you very much for having posted it!
This is not true!
Indeed the cathode for western blot is negatively charged (-) and the anode is positively charged (+) here. It is a common confusion for students, and I remember me asking the same question in one course... (the confusion comes from the fact that cations are positively charged, right?)
Best
Henrik
@@henrikslab I apologize you are right ! Thank you Henrik!
@@MrWhite-fm4lj No need for that... it is a classical confusion!
@@henrikslab 😄😉
ajudou muito, vlw🙃
Shukriya
Anyone here because of MCAT prep? oooh well, maybe just me
Love ya
Thanksss
Thanks a lot 🤗
Thank you bruh the WSSP does not explain any of the processes well
Hi Henrik! This technique seems strikingly similar to an enzyme-linked immunoassay test. Is it non the same?
A ver isto pra aula de bcm da nova
讚
LEER LOS ARTICULOS después de ver tus videos jajaja, es ley.
you just save me
Good meme
Die videos auf deutsch wären nett :)