Making injection port lids with micron filters for mycology
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- Опубліковано 6 лют 2025
- This video demonstrates how we make our own injection port lids with filters! We use these to produce liquid cultures and grain spawn : )
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MUSHLOVE
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2023 and I just got my lid materials in today. For anyone wondering, these specimens that he is using are rather large. They look to be extremely high quality. These injection ports are going to need around a 1/2" (12.7mm) hole to fit in to. The luer lock filter disk look professional but, again, they are much larger around than I would have expected.
Keep up the great work Gary! Love your videos! ♥️🍄
When installing the hole in the lid, I noticed you ran the drill motor in reverse and melted your way through the lid. Is that, in fact, your technique?
Most people recommend drilling through plastic in reverse to minimize mess and grabbing. Just personal preference.
Hi Gary. Does the syringe filter allow sufficient gas exchange?
yes for a liquid culture definitely, for a larger grain jar it might be a little slower but it does work
put the plastic lid on a jar and set your drill really low and put force on it, once its pierced through pull it out and turn the drill on. itll create a perfect hole and the plastic gets pulled out with the bit.
Hi. Which port would you use for sucking, and which one for injecting?
Is the gray one for injecting, and red one for sucking a LC syringe out of the jar?
the same port (the gray one) is used for both injecting and taking samples out - the red one is a filter disk so as you displace the air by adding or subtracting liquid it keeps the system sterile
@ ahhhh that makes a lot of sense. Thank you for that!
If I use a metal lid and flip it upside down, does it still make a good seal? Also im curious about what happens if you dont flip it?
Yes I usually use them for a couple runs until
they rust out. If you don’t flip they will make a canning-like seal and to open them you will have to break the vacuum which isn’t ideal. It’s better flipped or if you add a syringe filter it will help to displace the air through the filter so it won’t make a vacuum
@@FreshfromtheFarmFungi thank you so much for the reply. I have everything ordered and planning on making my own agar, grain spawn jars, and LC. I got the basics down but it's time I level up.. MUSH LOVE
I love your channel and all the knowledge you give away freely is incredibly valuable!
What are the advantages of using syringe filters for grain jars versus filter discs or polyfill?
check out our video with mushroom cult (fluid freakout fungi friday #11) there is a lot of info there!
Hello. If am using those same syringe filter for cordyceps cultivation. Can I reuse those filters next time or should I change after each harvest. Same question for liquid culture too
you can reuse them until they get plugged they will eventually turn off white and then need to be replaced
is the silicon save? Wont be releasing some chemicals, when heated?
You can use without silicon but it helps preventing contams from getting in and it doesn’t come into contact with the mycelium only for a brief time when pulling with syringes.
Thank's bro.
Have you used just micropore tape over a drilled hole?
yes it works with a few layers but will break down quickly
I stupidly flamed my needle but didn't let it cool off enough, so it melted a hole in the port, and kept it from "healing". Not visible but large enough to allow contam. Having a hard time finding plastic lids
Walmart. Has plastic ball lids both wide mouth and regular around 8 $ for 6. They also have there own brand that are white plastic for like$ 3.50 for 8 lids regular mouth though .
Sorry if this is a dumb question, but I am new to all of this.... I'm not sure what the syringe size applies to.
If I plan on using a size 15mm or 18mm syringe needle, do I need a size 25mm syringe filter?
Hopefully you've figured it out by now but you're mixing up all your numbers. The syringe gauge has nothing to do with either the self-healing port or the syringe filter (which is for gas exchanged not the needle).
How do you find the white plastic lids to hold up in the pressure canner/sterilizer ? Have you had them awhile and have they warped? I just got a bunch of them due to my metal lids rusting. I may start spraying a layer of clear coat before hand to help prevent rust after I puncture the holes
Just make sure they are pp5 rated (there will be a small triangle with a 5 in it)
Hoe come you said to flip around the metal ones? What mm are those filters? The phobic ones. Thanks you use same mm for lc and grain
.2 micron, I prefer glass but they all work the glass will just last longer and if you don’t flip the metal lids it will create a seal so for grain jars it’s harder to get the lid off and can create a mess
Gary great video as always. Question 1: you made a video about a month ago about the different type of agar dishes used for testing your area for different types of contamination and you were going to go over the results but never did. I was wondering if you were ever going to come back to the results?
Question 2: I have seen some people use MEAC agar and was wondering if the activated carbon actually does anything for the mycelium growth or does the carbon just make the agar black for better mycelium growth?
Hey thanks for watching! The plates we use to do our QC were negative - which means there is no growth on the plates so that is why I never posted the results. I will be making more LC's with these new lids and will be testing those as well! As far as activated charcoal, I have used it in the past with plant tissue culture and the reason we used it was to absorb excess exudates from the plants which affected the pH of the media. The charcoal was more of a buffer to absorb excess exudates. That being said, there may be applications in mycology related to this idea. If some mycelium releases exudates that affect the pH of the media, it might help to add activated charcoal. In this regard, it would be promoting healthier mycelium because it would keep the environment more stable for better growth. (there may be other applications that I am not aware of) MUSHLOVE
Does the syringe filter provide enough FAE for the grain spawn?
Don’t need FAE for colonization... just gas exchange. So no and yes... u won’t get much fresh air exchange but ull get the sufficient gas exchange needed for colonizing the grain.
Also FAE during colonization can dry out your grains so best to avoid it... a little gas exchange is all that’s needed
Where to get ports
PS..a 'forstner bit' will make a clean hole with no ragged edges...
thanks for the tip! ha
Thanks!
Hello Gary, do you use gaskets with the plastic lids? (I understand they’re not airtight.) Or does parafilm suffice? Thanks for such quality info and education!
I do not use gaskets for plastic lids in my experience if you pull them out while still warm from the autoclave/pressure cooker you can tighten them down and get a good seal since the plastic is malleable at that time
Hello. Where can I buy the same red filters? can you give a link please
I got them on amazon, 0.22 micron filters
@@FreshfromtheFarmFungi thanks
@@FreshfromtheFarmFungi why did you choose the 25 mm diameter? Have you tried 13 mm?
Good morning 🌞.there are 13mm filters on sale. you have a 25 mm diameter in your video. I wanted to ask why I bought these 25mm? 13 mm worse?
@@MegaUrod both work fine I think I got them for a good price but I actually use the smaller ones as well
Do you need to silicone the fabricated injection port in? Isn’t the friction fit enough? I’m scared because I haven’t put silicone on mine 😐
Matthew Taudevin It might be overkill yes - We have been using some without it with no issues but my thoughts were for long-term usage and might just replace them as they get worn out anyway (the ports that is)
@@justinlangley9529 Im gonna go the gasket route, white lids w/ silicone gasket....have you had any gunk problems there??
Got me some ports, and syringe filters and am gonna make some lids on white plastic.....not sure if i should epoxy SF though.
Thanks
Not needed it should be a tight fight and create a air tight seal, once worn just pop out and pop a new one in
You hsould try headvy duty injection ports. They're gonna stay sealed for much longer.
can you say which lids you purchase to make the lids?
they were plastic generic ball lids from amazon - any plastic pp4 and up should work (canning lids)
My man Gary at it again! Yeah those metal lids can be a pain sometimes trying to get a nice drill hole. Seems to depend on the brand (or rather how thick the lid is). Keep up the great work! "Mush love", as they say. ;)
I just sterilized everything, used honey water and then put some Microport tape (doubled) over the hole.
it would be better to put the micropore tape on first before sterilizing
Do the filters holdup in the pressure cooker?
Been using these for about 6 months running sterilizations once a week so far so good 👍🏻
Fresh from the Farm Fungi LLC Thanks! I just bought a bunch.🤘
Hey Gary, the max operating temperature for those syringe filters is 80 C. They are not designed to withstand pressure cooking/sterilization at 15 psi for 25 minutes. Any suggestions?
they worthless thats why no answer.
What art of glue for the filter
RV silicon
Super cool channel !
I recently found out the plastic lids are meant for freezer use, and allow air exchange when not used with the gasket ring. Since the gasket rings are painful to use/ fall in your mason jar from time to time, a bead of RVT along the circumference does the job better.
Thanks for the insights! I have been using these for 6+ months still working great (about 25 sterilization runs) No gasket just tightened up firmly - although for LC’s it might help a lot to ad the gasket MUSHLOVE
awesome thank you!
I hate having to fish around and find the hole....
What size are the injection ports?
Im not sure most sizes should work though just make sure it doesn’t interfere with the threading of the lid
Thats a FUCK TON of silicone. Lol
Dude, I think you had that drill (large bit) in reverse.
Yes it was on purpose - to prevent the plastic from cracking
You can prevent cracking using drill bits made for wood. Don't use metal bits for plastic in reverse...@@FreshfromtheFarmFungi
Legit video,
wouldnt hire you for a silicon job haha
I hope you can drill better now :P
my friend Zack makes the lids for me now he has a drill press and better setup for this now