Ouuu, you mentioned most of my mushroom people: You, Jewel funkyfungi , Tony freshcup, mosecreek, southwest mushrooms (that guy), renegade, Earth angel, ... Southwest mushrooms is epic thou
Southwest mushrooms was the one that got me interested in growing mushrooms and I've been watching him for awhile now. I recently discovered that his warehouse is 1 mile up the road from my house
First, of all Hardy does make great products. Secondly, I never knew about these QC processes. Which is strange since I work in GD&T Quality Control (Metrology) as a day job. I've seen all the other options for pre-poured agar plates at Hardy but didn't think it was anything I needed. Now, I'm thinking we need mushroom farm specific bi/tri-plates for QC.
This is awesome. Colledgich Educamation! Appreciate it so much. Mush love to you. Thanks again. I would never have the opportunity to understand any of this if you did not give it. Thanks again! I watch Paul Stamets's videos and have a glimmer of what he is talking about. Yours I flatout can understand.
I was curious about the MEA from Hardy. The Malt Extract Agr w/ 0.01% Chloramphenicol are so much cheaper. Does it matter for the mycelium if the antibiotic is in there? Thanks!
Sell different kinds of agar. Sell syringes . Leave an agar plate out for 15 minutes next to the humidifier to test which colonies of contamination are rampant. These will be the majority contaminants. Great video!
this will prevent condensation from dripping from the lid to the surface of the agar which can spread the sample and confuse the point of the test. It isn’t necessary if you are careful and understand this issue.
Hi Gary. Thanks for a great video. I learned a lot. I am curious about what you do at around 13:59 in the video. You drop the liquid on the agar plate, then you *turn the plate upside down*. I’m new to agar and I’ve been confused by my recent reading. There are differing opinions on plates “right side up” and “upside down”. There are different views on what is best for storing virgin plates, and what is best after inoculation, when you incubate the plates. So: please can you give your view on what is best in what situation, and why? Kind regards, and thanks in advance, Schalk
Great video. I am going to order some plates today. One question however: I understand the testing function and how it works. But I can't wrap my head around the mechanism which causes the agar to refresh a tired strain? It is my (very limited) understanding that fade off is caused by genetic issues such as telomere shortening, genetic drift, etc. The agar should just be providing optimized nutrition, combined possibly with some protection from competition when the agar is selective. My query is completely out of curiosity. You are far further down the mycological road than I am. So I am not saying you are incorrect. Just that I am always interested in finding out why my understanding differs from others who are more familiar with a subject than I.
yes senescence is caused by these spontaneous mutations but also can be accelerated by a lack of good nutrition over time. Changing nutrients every so often seems to bolster the mycelium - it reacts to the new environment and maybe learns how to create new enzymes to break the different sugars down. It just is an observation - I haven’t actually sequenced the dna before and after growing in different medias but I have a hunch there are mini gene segments that get activated or created with new sources of nutrients- hope that makes sense! 🍄❤️🙏🏻
@@FreshfromtheFarmFungi It does, thank you for the reply. It is an interesting theory. I can't think of any reason to doubt it off the top of my head. We are learning more all the time about the short-term evolutionary process in individuals that seems to work alongside longer-term darwinian evolution in populations of individuals. This short-term system that responds to the immediate environment is active in humans and plants. Our genes do respond to environmental challenges within a single lifetime. But I would imagine it is probably even more important in a fungus where a colony functions as both an individual AND a colony at the same time. Mainly because it reproduces both sexually and asexually. I love your science-based approach to mycology. Keep up the great content. I don't typically subscribe to youtube channels as a rule. I am making an exception in your case. Respect and best wishes.
Hi I have a question. Could you clarify why do you flip your agar over after the introduction of the liquid culture and do you store the plates upside down. Does the mycelium grows on plates upside down. And why is this better in comparison to plates stored with lid up. Thank you :)
Yes they grow upside down fine. It’s to protect the mycelium from condensation and also a habit from working in the clinical labs where we would read plates agar side up to look for hemolysis and color indicators. It’s best practice.
That's all great but most of us cannot buy anything from Hardy Diagnostics because we don't own companies. Where else can we buy high quality agar dishes?
amazon - check our affiliate store here Check out this page from Gary Heferle www.amazon.com/shop/influencer-a88d1601?ref=cm_sw_sm_r_inf_pub_influencer-a88d1601__mw_G1FThVir6Wy9h
Very cool, and informative, thank you for sharing the knowledge. Also thank you for the liquid culture, just received this week. Its nice, ordering your products knowing that I'm getting the cleanest cultures in town.
Gary- for production, do you order MEA plates pre-poured or pour them yourself? From the video it sounded like you might order them and that was surprising to me. Time saver? quality assurance? Something else? Seems like it would be expensive, but maybe I’m underestimating value of time in commercial operation.
Troy Lerner We pour our own plates for production but order most of the quality control plates because they come with certificates of qc and are ISO certified. Also, we have some great sources so the costs are reasonable when ordering a sleeve here and there compared to the MEA/PDA plates which we pour A LOT of and buy bulk mixed agar and plates by the 500 counts - MUSHLOVE
That flow hood though. This is random, but one thing I have been wondering... I notice basically everyone uses the cheap stirring plates, from chemists to mycologists, etc. With there being so many out there and having a lot of features, is it just not worth spending on higher quality stir plates with things like a hot plate built in, etc.? Seems obvious, why waste money if it works, but I would think some people would have them, but I am yet to see a stirring plate of high quality outside what I use in my schools labs.
The hot plate stir plate has its uses - like when adding antibiotics the temperature is sensitive and making more controlled media like pH’ing for mycorrhizal species but for these simple recipes and making liquid cultures a regular stir plate suffices
Here is a look inside the tent ua-cam.com/video/ByQhtML5Ceo/v-deo.html If you're in the area we are hosting a 3 hour bulk substrate prep and fruiting class February 15th www.mushroomcult.net for more info MUSHLOVE
Could you use two different coloured agars and put more nutrients in one colour so that the plate could be used for art? Like a smiley face? Would be way cool
I wrap them in the original sleeve or poly sleeve and put them in the fridge agar side up they will be good for 6 months or so until they dry out eventually
There really isn’t a button to this rabbit hole is to here?😰 lol, awesome video, very informative, thank you for giving me more tools for the trade!✌🏼👽☮️🍄❤️
Hey Gary...thanks for your information, especially the clinical side of looking for differentials. I noticed you stored your plates in what appears to be a small box. Are you QC testing them in total darkness and if so, what temperature have you found works best? I appreciate your help. Rob
@@FreshfromtheFarmFungi Hey Gary, thanks for the info. I rewatched the video and wanted to know, that when you were discussing the v-8 fruit juice agar plates used for testing for the presence of Trichophytons, you mentioned that they would also be used to check some of the "old and tired" strains as for their viability? I ask because I have collected several LC samples that have been under refrigeration for about 4 months....should I even waste my time pouring the plates or not? Everything I have read regarding agar plates and storage says 6 months maximum....Can you shed some of your light on this?
I'm curious about your use of HEA and Blood Agar. Do you ever see mycelium growth on these media or are they strongly selective to bacteria? DRBC seems to be strongly selective to fungi but not to any particular type so one would need to incubate molds, for example, until they sporulate to identify them from the mushroom culture. Do you just put them in your incubator to accelerate the process and know that after a certain amount of time any mycelium growth is not mold?
Some fungi/mycelium may be resistant yes, but in general those plates are meant to be read earlier (24-48 hours) to decipher bacterial colonies. The most definitive way to verify mycelium is to grow it and fruit it out or perform genetic tests. Mold will sporulate yes and has specific morphology and characteristics. You can also identify microscopically but my experience is to just grow them out and isolate and then keep the process sterile to ensure good results.
@@FreshfromtheFarmFungi Thanks for your reply! I'm assuming 24-48 hours in an incubator at ~28c for the bacterial plates. Same for the DRBC to identify yeast? Identifying mold vs mushroom mycelium under the microscope isn't something I'm good at yet, perhaps I'm just missing some key details. Do you know of a media that is selective to molds (specifically Trichoderma and Penicillium) or do you think it's just better to wait for color changes due to sporulation? My thought is to increase the accuracy and reduce the turnaround in QC of liquid cultures with more selective media.
Thanks! Can I ask you something? Is it normal that my parafilm is very easily detached from the separating layer? It's almost always separated when I dispense it.Thus I'm a bit concerned about how sterile my dishes are when I seal them with this tape.
It can happen with older batches, it shouldn’t effect them if you are using good technique- parafim is not meant to be sterile just to keep moisture locked in and lids on tight - if moisture gets onto the seal and back into the plate then I would be concerned
Garry could you please share your knowledge on this subject i hear other bloggers talking about this and i have no idea what they're talking about what i'm referring to is isolated spores or isolated spore samples and non isolated spore samples could you please share some of your expertise and knowledge regarding this topic thank you Sir
I am making a video on breeding and this topic will be covered. Spore isolation is important in breeding to methodically introduce haploid mycelium, to form full diploid heterozygous mycelium, which produces fruits. More to come! MUSHLOVE
Could i get some reference materials to essentially teach myself so clinical mycology? Ive gotten a couple text books and a teach yourself kind of study guide so far
Motor Miljø It’s a thermo scientific laboratory grade incubator set at 72F usually runs between 72-74 - like this but older www.globalindustrial.com/p/medical-lab/laboratory-equipment/le-laboratory-shakers/imc18-compact-microbiological-incubator-0-65-cu-ft-100-240v?infoParam.campaignId=T9F&gclid=Cj0KCQiAmsrxBRDaARIsANyiD1rvi_tWyny6iZbA0FWdLUEoCjVP_TxRBR_vn4rTM3XzzS83Y5PS700aAm9jEALw_wcB
The hektoen and blood agar aren't bad, $12-16 for 10 plates. But the v9 and byce?? Yikes! Those are $9-$12 per plate... sold in packs of 10. So those 5 ten-packs cost about $263.
yes or you can make them but it will cost significantly more for a flow hood, all the lab supplies etc. or just numbers game by pouring them in an SAB - there are options but they all come with a cost or time commitment
@@FreshfromtheFarmFungiWhat I'm saying is... why are 10 hektoen plates $16, but 10 V9 agar plates are $92? Are hektoen and blood agar super easy to make, and byce and v9 are super difficult to make? Im assuming they're all made using expensive equipment, but some are 10-15x more expensive... for ingredients that are cheap.
Interested in picking up a pack of each of these so I can properly QC my liquid cultures. I'm new getting into this as a hobby, and would love to keep it clean! Can you by chance provide links or the name exactly of the plates you use/order? I opened Hardy Diagnostics and their selection is HUGE! Appreciate any help! Mush Love!
I would reach out to them for more information - there are so many techniques for QC I don't want to narrow the scope because they have so many great products! MUSHLOVE
@@FreshfromtheFarmFungi Thank you! Called and spoke to them, NICEST PEOPLE EVER! They watched your video even and they were super happy! Appreciate everything, making sure safety is #1 and testing for these contams will surely ensure I keep that my priority. Mush Love!
@Fresh from the Farm Fungi but the thing I don't understand brother is how do you get the fungus ie mushroom seeds from the mushroom itself and how do you put it in these solutions and how long do you mix them to get the final result
@@FreshfromtheFarmFungi nice!! Thank you for putting in the time and effort with these videos and the community!! And thanks for replying, have a great week. Mush love 🍄🧠🌱
I went to school for clinical lab science - I studied a lot of clinical mycology but not necessarily field mycology. I gained an interest in mushroom cultivation later on after working in labs for a while
Hey man much respect for your hard work and devotion , I understand getting lab quality agar and ordering from respectable sources but where’s the cat food agar or fish food agar 🧫?
@@FreshfromtheFarmFungi wow haha, I wasn't expecting a reply on a months old video... Awesome! Hey, I've only recently gotten interested in mycology, but I think the I'd be most interested in trying to identify new species. Are there any mycologists that try to focus on that?
@@fusion9619 Yes, there are plenty of facebook groups that are fixated on this topic as well but a good mycologist who is in this realm is Allen Rockefeller (I think spelled that way) he is very active in this field and could be a good resource - also check out Tradd Cotter and Mushroom Mountain - they teach classes on the subject of identification and foraging Thanks for watching and following along! MUSHLOVE
@@13LuckyykcuL13 in formal education it is pronounced how I pronounce it - a lot of internet teachers pronounce it like that - apples to apples in my opinion
Fascinating, the rabbit hole deepens. Thanks for sharing your knowledge.
This guy is worth millions...litteraly
Amazing video!!!
I'm just starting on agar work and this was so helpful. I can't express my gratitude in words!
You The Man Gary!.
Thanks for turning the camera! Thanks for the content
Keep kicking ass Gary, love the content.
Much love!! Between you and PGT, I dont think I need any other influences. Thank you!!
thanks for watching! 🍄❤️
@@FreshfromtheFarmFungi you're welcome. Have a good one!!
Greetings and thanks from London, England!
wow this was one of the coolest things ive learned. i wish i had this knowledge at my last rental home
Your a great teacher thanks 😊 🙏
Ouuu, you mentioned most of my mushroom people: You, Jewel funkyfungi , Tony freshcup, mosecreek, southwest mushrooms (that guy), renegade, Earth angel, ...
Southwest mushrooms is epic thou
Southwest mushrooms was the one that got me interested in growing mushrooms and I've been watching him for awhile now. I recently discovered that his warehouse is 1 mile up the road from my house
Thank you so much.
Thanks for the info 👍👍
So interesting! Mycology has made it so I want to study biology
This was awesome. I am new to agar and didn't know anything about selective or differential media. So cool.
First, of all Hardy does make great products. Secondly, I never knew about these QC processes. Which is strange since I work in GD&T Quality Control (Metrology) as a day job. I've seen all the other options for pre-poured agar plates at Hardy but didn't think it was anything I needed. Now, I'm thinking we need mushroom farm specific bi/tri-plates for QC.
their prices are a bit crazy... over $100 for some agar powder.. I suppose if you're a legit lab but definitely not for the journeyman mycologist.
The bit about leigonella (?lol) is so helpful! Im immuno compomised and have asthma. Im going to have to grab some of those plates
This is awesome. Colledgich Educamation! Appreciate it so much. Mush love to you. Thanks again. I would never have the opportunity to understand any of this if you did not give it. Thanks again!
I watch Paul Stamets's videos and have a glimmer of what he is talking about. Yours I flatout can understand.
Great information! I started watching many videos on mushrooms, your video on agars and their uses explained so mush🌈😃🤙
Thanks!
Very informative! Thanks
thank you for sharing your expert practices.
Much love from Liverpool =) love your vids, please share more
thanks a lot, more science for the masses
Ya aught to adopt mossy creeks shelving units. Were running those same black shelves, and they are a pain to clean under.
Yes we have moved on from those shelves and made our own pvc ones to streamline cleaning 👍😎
very cool i never knew about this
Just want to say, if you had a patreon, I'd totally support it :)
👍👍🎯from 🇨🇦
thanks for all the info very helpful
I was curious about the MEA from Hardy. The Malt Extract Agr w/ 0.01% Chloramphenicol are so much cheaper. Does it matter for the mycelium if the antibiotic is in there? Thanks!
Thanks. Appreciated the video.
Isn't a good idea to clean the growing area with ozone lamp reguralry?
Subscribed and motivated to study harder. Thank you brother
Excellent video - watching closely - keep it up!
what do you guys think about using pasta water as a substitute for potato water
How do you know if your culture/mycelium is getting weak?
Thanks for the great video!
Sell different kinds of agar. Sell syringes . Leave an agar plate out for 15 minutes next to the humidifier to test which colonies of contamination are rampant. These will be the majority contaminants. Great video!
After you put 2-3 drops of LC on your plates, why flip the plates upside down? I am new to this and just trying to understand. Thanks.
this will prevent condensation from dripping from the lid to the surface of the agar which can spread the sample and confuse the point of the test. It isn’t necessary if you are careful and understand this issue.
Thanks for this good content. Di you use pigments for coloration?
sometimes I do now but this video is about how these plates can be confusing because of the colors from the actual media without dyes
Nice vid, very informative!
What kind of humidifier and tents do you use for grow spaces?
its all on our amazon affiliate page but here they are amzn.to/3TidjLY for fruiting tent amzn.to/3cqC2gD for the humidifier
Hi Gary.
Thanks for a great video. I learned a lot.
I am curious about what you do at around 13:59 in the video. You drop the liquid on the agar plate, then you *turn the plate upside down*. I’m new to agar and I’ve been confused by my recent reading. There are differing opinions on plates “right side up” and “upside down”. There are different views on what is best for storing virgin plates, and what is best after inoculation, when you incubate the plates.
So: please can you give your view on what is best in what situation, and why?
Kind regards, and thanks in advance,
Schalk
Finally some science.
I wish more micro leaders went more in-depth about agar more often it’s so important apparently
Great video. I am going to order some plates today. One question however:
I understand the testing function and how it works. But I can't wrap my head around the mechanism which causes the agar to refresh a tired strain? It is my (very limited) understanding that fade off is caused by genetic issues such as telomere shortening, genetic drift, etc. The agar should just be providing optimized nutrition, combined possibly with some protection from competition when the agar is selective.
My query is completely out of curiosity. You are far further down the mycological road than I am. So I am not saying you are incorrect. Just that I am always interested in finding out why my understanding differs from others who are more familiar with a subject than I.
yes senescence is caused by these spontaneous mutations but also can be accelerated by a lack of good nutrition over time. Changing nutrients every so often seems to bolster the mycelium - it reacts to the new environment and maybe learns how to create new enzymes to break the different sugars down. It just is an observation - I haven’t actually sequenced the dna before and after growing in different medias but I have a hunch there are mini gene segments that get activated or created with new sources of nutrients- hope that makes sense! 🍄❤️🙏🏻
@@FreshfromtheFarmFungi It does, thank you for the reply. It is an interesting theory. I can't think of any reason to doubt it off the top of my head. We are learning more all the time about the short-term evolutionary process in individuals that seems to work alongside longer-term darwinian evolution in populations of individuals.
This short-term system that responds to the immediate environment is active in humans and plants. Our genes do respond to environmental challenges within a single lifetime. But I would imagine it is probably even more important in a fungus where a colony functions as both an individual AND a colony at the same time. Mainly because it reproduces both sexually and asexually.
I love your science-based approach to mycology. Keep up the great content. I don't typically subscribe to youtube channels as a rule. I am making an exception in your case. Respect and best wishes.
Good job keep going.
Hi I have a question. Could you clarify why do you flip your agar over after the introduction of the liquid culture and do you store the plates upside down. Does the mycelium grows on plates upside down. And why is this better in comparison to plates stored with lid up. Thank you :)
Yes they grow upside down fine. It’s to protect the mycelium from condensation and also a habit from working in the clinical labs where we would read plates agar side up to look for hemolysis and color indicators. It’s best practice.
@@FreshfromtheFarmFungi Thank you so much.
I follow you on Tik Tok 😂 I’m 16 learning more everyday. I enjoy your videos.
thanks for following along and glad you are starting your journey! MUSHLOVE
How and with what do you clean your humidifier? And shouldn't you also need to clean the air hose? Thanks for this video it was very insightful
ua-cam.com/video/J2CKOQVroG0/v-deo.html here is my process yes I do it all 👍
That's all great but most of us cannot buy anything from Hardy Diagnostics because we don't own companies. Where else can we buy high quality agar dishes?
amazon - check our affiliate store here Check out this page from Gary Heferle www.amazon.com/shop/influencer-a88d1601?ref=cm_sw_sm_r_inf_pub_influencer-a88d1601__mw_G1FThVir6Wy9h
What is the best way and how long can the unused plates be stored?
Very cool, and informative, thank you for sharing the knowledge. Also thank you for the liquid culture, just received this week. Its nice, ordering your products knowing that I'm getting the cleanest cultures in town.
That's an unfair assumption. You can just say his technique is perfect without putting someone else down.
Gary- for production, do you order MEA plates pre-poured or pour them yourself? From the video it sounded like you might order them and that was surprising to me. Time saver? quality assurance? Something else? Seems like it would be expensive, but maybe I’m underestimating value of time in commercial operation.
Troy Lerner We pour our own plates for production but order most of the quality control plates because they come with certificates of qc and are ISO certified. Also, we have some great sources so the costs are reasonable when ordering a sleeve here and there compared to the MEA/PDA plates which we pour A LOT of and buy bulk mixed agar and plates by the 500 counts - MUSHLOVE
What is the best temp to keep inoculated plates at?
That flow hood though. This is random, but one thing I have been wondering... I notice basically everyone uses the cheap stirring plates, from chemists to mycologists, etc. With there being so many out there and having a lot of features, is it just not worth spending on higher quality stir plates with things like a hot plate built in, etc.? Seems obvious, why waste money if it works, but I would think some people would have them, but I am yet to see a stirring plate of high quality outside what I use in my schools labs.
The hot plate stir plate has its uses - like when adding antibiotics the temperature is sensitive and making more controlled media like pH’ing for mycorrhizal species but for these simple recipes and making liquid cultures a regular stir plate suffices
Hey mang, great vids...can you post a link to that quick dissolve mea?
Thanks!
Take a shot every time he says "Auger" you'll be getting hammered in about 1 minute
if I had a nickel every time someone commented on my NY pronunciation 🤌
Can I put my v9 ager to Grain spawn without having any problems fruiting?
yes
AGAR NOT AUGER THIS IS THE THIRD PERSON TO MAKE THIS MISTAKE ITS NOT HARD in all seriousness this video was very helpful
Love to get more info on your growing setup.
Here is a look inside the tent ua-cam.com/video/ByQhtML5Ceo/v-deo.html If you're in the area we are hosting a 3 hour bulk substrate prep and fruiting class February 15th www.mushroomcult.net for more info MUSHLOVE
Hardy diagnostic prices are no joke. Over $100 for some Agar...
you get what you pay for
Have u ever used cornstarch as an agar substitute?
Could you use two different coloured agars and put more nutrients in one colour so that the plate could be used for art? Like a smiley face? Would be way cool
perhaps 🤔
always providing great info. how do you store your plates (for weeks/months) before you use them?
I wrap them in the original sleeve or poly sleeve and put them in the fridge agar side up they will be good for 6 months or so until they dry out eventually
@@FreshfromtheFarmFungi awesome. thank you!
On legionella, the hvac system it’s known for is evaporative cooling or “swamp coolers.” Also, open loop cooling systems. Tidbit of knowledge
thanks for the insight we steer clear of swamp coolers because of this! 🍄❤️
@@FreshfromtheFarmFungi Great content btw, really appreciate the perspective!
Has anyone done any tests to see which agar recipe makes mycelium grow the fastest?
not that I am aware of - the standards are MEA/PDA I am testing a couple new recipes but I think changing up
variety is key long term
Video is genius!
thanks for watching! MUSHLOVE
There really isn’t a button to this rabbit hole is to here?😰 lol, awesome video, very informative, thank you for giving me more tools for the trade!✌🏼👽☮️🍄❤️
nice info m8
Hey Gary...thanks for your information, especially the clinical side of looking for differentials. I noticed you stored your plates in what appears to be a small box. Are you QC testing them in total darkness and if so, what temperature have you found works best? I appreciate your help. Rob
thanks it’s an incubator set to 72F (stable temps are important and warmer temps are faster growth to a point)
@@FreshfromtheFarmFungi Hey Gary, thanks for the info. I rewatched the video and wanted to know, that when you were discussing the v-8 fruit juice agar plates used for testing for the presence of Trichophytons, you mentioned that they would also be used to check some of the "old and tired" strains as for their viability? I ask because I have collected several LC samples that have been under refrigeration for about 4 months....should I even waste my time pouring the plates or not? Everything I have read regarding agar plates and storage says 6 months maximum....Can you shed some of your light on this?
I'm curious about your use of HEA and Blood Agar. Do you ever see mycelium growth on these media or are they strongly selective to bacteria? DRBC seems to be strongly selective to fungi but not to any particular type so one would need to incubate molds, for example, until they sporulate to identify them from the mushroom culture. Do you just put them in your incubator to accelerate the process and know that after a certain amount of time any mycelium growth is not mold?
Some fungi/mycelium may be resistant yes, but in general those plates are meant to be read earlier (24-48 hours) to decipher bacterial colonies. The most definitive way to verify mycelium is to grow it and fruit it out or perform genetic tests. Mold will sporulate yes and has specific morphology and characteristics. You can also identify microscopically but my experience is to just grow them out and isolate and then keep the process sterile to ensure good results.
@@FreshfromtheFarmFungi Thanks for your reply! I'm assuming 24-48 hours in an incubator at ~28c for the bacterial plates. Same for the DRBC to identify yeast? Identifying mold vs mushroom mycelium under the microscope isn't something I'm good at yet, perhaps I'm just missing some key details. Do you know of a media that is selective to molds (specifically Trichoderma and Penicillium) or do you think it's just better to wait for color changes due to sporulation? My thought is to increase the accuracy and reduce the turnaround in QC of liquid cultures with more selective media.
Do you have an agar recipe for slow growing strains?
I am fond of V9 or BCYA for those - or run them in LC
@@FreshfromtheFarmFungi ok cool
Thanks!
Can I ask you something? Is it normal that my parafilm is very easily detached from the separating layer? It's almost always separated when I dispense it.Thus I'm a bit concerned about how sterile my dishes are when I seal them with this tape.
It can happen with older batches, it shouldn’t effect them if you are using good technique- parafim is not meant to be sterile just to keep moisture locked in and lids on tight - if moisture gets onto the seal and back into the plate then I would be concerned
Garry could you please share your knowledge on this subject i hear other bloggers talking about this and i have no idea what they're talking about what i'm referring to is isolated spores or isolated spore samples and non isolated spore samples could you please share some of your expertise and knowledge regarding this topic thank you Sir
I am making a video on breeding and this topic will be covered. Spore isolation is important in breeding to methodically introduce haploid mycelium, to form full diploid heterozygous mycelium, which produces fruits. More to come! MUSHLOVE
Could i get some reference materials to essentially teach myself so clinical mycology? Ive gotten a couple text books and a teach yourself kind of study guide so far
I mostly learned with experience working in labs but there are some clinical books out there yes
What's your incubator setup for the plates?
Greetings Tor
Motor Miljø It’s a thermo scientific laboratory grade incubator set at 72F usually runs between 72-74 - like this but older
www.globalindustrial.com/p/medical-lab/laboratory-equipment/le-laboratory-shakers/imc18-compact-microbiological-incubator-0-65-cu-ft-100-240v?infoParam.campaignId=T9F&gclid=Cj0KCQiAmsrxBRDaARIsANyiD1rvi_tWyny6iZbA0FWdLUEoCjVP_TxRBR_vn4rTM3XzzS83Y5PS700aAm9jEALw_wcB
The hektoen and blood agar aren't bad, $12-16 for 10 plates. But the v9 and byce?? Yikes! Those are $9-$12 per plate... sold in packs of 10.
So those 5 ten-packs cost about $263.
yes or you can make them but it will
cost significantly more for a flow hood, all the lab supplies etc. or just numbers game by pouring them in an SAB - there are options but they all come with a cost or time commitment
@@FreshfromtheFarmFungiWhat I'm saying is... why are 10 hektoen plates $16, but 10 V9 agar plates are $92?
Are hektoen and blood agar super easy to make, and byce and v9 are super difficult to make?
Im assuming they're all made using expensive equipment, but some are 10-15x more expensive... for ingredients that are cheap.
Interested in picking up a pack of each of these so I can properly QC my liquid cultures. I'm new getting into this as a hobby, and would love to keep it clean! Can you by chance provide links or the name exactly of the plates you use/order? I opened Hardy Diagnostics and their selection is HUGE! Appreciate any help! Mush Love!
I would reach out to them for more information - there are so many techniques for QC I don't want to narrow the scope because they have so many great products! MUSHLOVE
@@FreshfromtheFarmFungi Thank you! Called and spoke to them, NICEST PEOPLE EVER! They watched your video even and they were super happy! Appreciate everything, making sure safety is #1 and testing for these contams will surely ensure I keep that my priority. Mush Love!
When I get cultures from you do I need to do these tests?
I perform qc on every batch, I would suggest plating on agar to preserve the mycelium 🙂
Brother, what if you give me the names of those solutions and write to us the ingredients and how to combine them?
these are all Agar recipes you can look them up anywhere- PDA, MEA, BCYA, WA, V9, DRBC, Chrom
@Fresh from the Farm Fungi but the thing I don't understand brother is how do you get the fungus ie mushroom seeds from the mushroom itself and how do you put it in these solutions and how long do you mix them to get the final result
@@galbois3496 we clone the tissue like this and they last for 6-12 months ua-cam.com/video/N4Ch54EEzjo/v-deo.html
@@FreshfromtheFarmFungi ooh thanks so much bro 🙏🙏🙏
So these are for streak plates
will my mushrooms grow on all of these
Is there an Agar substitute
some people use gellan gum I haven’t ever tried it since agar works so well
How did that v9 agar work out for you?
It’s awesome, definitely a game changer I use it now when I pull from slants to start the cycle going
@@FreshfromtheFarmFungi nice!! Thank you for putting in the time and effort with these videos and the community!! And thanks for replying, have a great week. Mush love 🍄🧠🌱
Science.
the captions say Auger
its A-gaar
indeed
Do you wear normal shoes in there?
crocks
Did you go to school for mycology?
I went to school for clinical lab science - I studied a lot of clinical mycology but not necessarily field mycology. I gained an interest in mushroom cultivation later on after working in labs for a while
Sabouraud Agar is the way.
yes it is one more way indeed 👍
V8? Fruit juice? ;)
V like Vegetable
Is a tomato a fruit or vegetable? lol I guess I should have just said “juice” but you are correct V like vegetable - keeping things honest! MUSHLOVE
Hey man much respect for your hard work and devotion , I understand getting lab quality agar and ordering from respectable sources but where’s the cat food agar or fish food agar 🧫?
there are hundreds of recipes these are just a select few that I prefer.
Why the hell do some people look past the camera lense, are they looking atr themselves, it's so bloody of puting weird
auger.
What does a clinical mycologist do?
Identify fungal pathogens in clinical samples to help the pathologist
@@FreshfromtheFarmFungi wow haha, I wasn't expecting a reply on a months old video... Awesome! Hey, I've only recently gotten interested in mycology, but I think the I'd be most interested in trying to identify new species. Are there any mycologists that try to focus on that?
@@fusion9619 Yes, there are plenty of facebook groups that are fixated on this topic as well but a good mycologist who is in this realm is Allen Rockefeller (I think spelled that way) he is very active in this field and could be a good resource - also check out Tradd Cotter and Mushroom Mountain - they teach classes on the subject of identification and foraging
Thanks for watching and following along! MUSHLOVE
@@FreshfromtheFarmFungi thank you so much! I'm looking them up right now... Mushlove, haha
@@FreshfromtheFarmFungi oooh, looking at Mushroom Mountain, I just got a new favorite word - Mycoremediation
10 dollars a plate for V9. It's a bit much. I will pass.
Why do I like this stuff? I don't even like mushrooms!
They're worth money. That could be it.
Hot nerd alert! 🚨
It's pronounced Ay-gr
@@13LuckyykcuL13 in formal education it is pronounced how I pronounce it - a lot of internet teachers pronounce it like that - apples to apples in my opinion
Why can no one pronounce agar right? 😂
It just depends where you’re from - midwest says Ayyygar, East coast says Ahgar - they both in fact mean the same thing
Say what you mean: Quit arbitrarily using color, you look stupid to those of us who do this professionally.
It's agar not auger.