Regarding using the antibiotics for primary cells : Does is it necessary to add antibiotics whenever we change the media( let us say every 24 hours) for the first passage P0 , or it is recommended to add antibiotics just for the first 24 hours ?
Thanks for your comment! It is recommended to always add antibiotics(e.g Penicillin-Streptomycin) to prevent microbial contamination. But it depends ion the cell condition and cell property.
Hi.. Thanx for the introduction. I am working on cell lines production in Pakistan. Can you know exactly the abbreviation of SH- SY5Y stands for? These are neuroblastoma cell lines but I am looking for exact abbreviation.
Hi Popa, thanks for leaving a comment! Yes, for most cases, immortalized cells hold the same phenotype and genotype to their parent tissue, meaning they retain their original function. This reason, along with their extended replicative capacity, is why immortalized cell lines are more suited for tissue culture studies than primary cells. Hope this helps!
Hi Ajay, thanks for commenting and watching our video! When Primary Cells (cells which you get directly from tissue) are grown in vitro and use up all the available space or media for growth, they will stop proliferating. In order to keep the cells in the proliferation phase, we need to take some of these primary cells and grow them in a fresh dish - a process called sub-culturing. The resulting population of cells in this subculture are called secondary cell lines. So, there are as many examples of secondary cell lines as they are tissue/cell types in the body! If you want to learn more about cell culturing, we have an entire knowledge base here: goo.gl/3LR25m
Applied Biological Materials - abm Please give me any 2 example of secondary cell lines, finite cell lines and infinite cell lines if possible. just the names
Hi Diego! You’re correct in that DMEM, RPMI and F12 are chemically defined media. However, they are usually supplemented with FBS because FBS provides vital macromolecules, growth factors and immune molecules.
Hi Ameer, if you want to know the difference between embryonic and adult cell with regards to their proliferation, embryonic cells proliferate rapidly and differentiate into specialized cell types which can be categorized into three kinds: 1) Terminally differentiated cells eg. cardiac muscle cells that are no longer capable of cell division. (If lost, cannot be replaced via natural repair mechanism). 2) Adult differentiated cells kept in the G0 phase and divide as required for replenishing the dead cells such as skin fibroblasts, cells lining blood vessels and epithelial cells of most internal organs, such as the liver, pancreas, kidney, etc. Most cells belong to this category and some form of injury/need to repair cells induces these cells to divide. 3) The third category is pleuripotent stem cells (less differentiated cells) which include blood cells, epithelial cells of the skin and digestive tract lining which have short life spans and must be replaced by continual cell proliferation. These divide to produce cell line lineage-specific cell type and once they commit to a specific lineage, they do not divide anymore.
Hi Sophia, thanks for leaving a comment! Cell cloning is the process of getting a population of genetically identical daughter cells from one parent cell - if you are able to start cell culture from one cell in a dish (a common strategy to isolate a single cell is to make a very high dilution), the subsequent cells will all be identical clones of this original cell. This video is discussing cell culturing in general and not specifically about cell cloning technique. Cell culture is simply a technique to take cells from an organism and grow them in vitro (an artifical environment).
Thank you! Glad you enjoyed it! Please let us know if you have any questions about the material. Or, you can read more at our knowledge base: goo.gl/OtCzAJ
this guy is a fantastic talker. his voice is so soothing
I love everything about this video. Great refresher, calming voice, excellent images. Thank you for creating this!
Thanks for watching! ;) Glad that you found the video helpful!
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The best cell culture overview video on UA-cam.
Hi Sumita, thanks for watching our video! Glad you like it - please let us know if you have any questions!
5 min video explained a week of lectures thank you very much!!
Thanks for watching! Feel free to contact us if you have any questions.
Even after 7 years your video is the best!!
Thanks for watching! :)
Thank you for uploading this video. Very helpful for me who work with bacterial cell for 7 years and now work with mammalian cell.
You're welcome! ;) Glad you found the video helpful!
All necessary information in short video! Thank you! I feel myself as a professional after watching that 😅
It's very short, but interesting. Explains such difficult things in a simple way
Thank you for watching! Glad you found the video helpful :)
Thank you for helping me understand what cell culture is. I’m currently reading the immortal life of Henrietta lacks and came up upon this term.
No problem! ;) Glad you found the video helpful!
wow, actually really liked this video lol. Wasnt distracted by anything at all. Well done :D
Hello there,
Thanks for watching! Glad you found the video helpful! ;)
This is going to be great for my stem fair project
Fantastic. This video has perfectly made and explains everything in a short but productive manner. Thanks
Glad you enjoyed it!
Regarding using the antibiotics for primary cells : Does is it necessary to add antibiotics whenever we change the media( let us say every 24 hours) for the first passage P0 , or it is recommended to add antibiotics just for the first 24 hours ?
Thanks for your comment! It is recommended to always add antibiotics(e.g Penicillin-Streptomycin) to prevent microbial contamination. But it depends ion the cell condition and cell property.
Informative, concise and illustrative.
Thanks for watching! :)
do you provide technical know how to establish tissue culture lab
Amazing! Lucid and lucrative. Thanks a lot
Thanks for your nice comment, Mustafa. We're glad to hear that! :)
Amazing video ! thank you so much !
Hi Nour, you're welcome! Glad to see that you enjoyed our video :)
Thanks for sharing this great video.
Thanks for watching!
I was looking for these informations, thanks a lot :))
Glad to hear that you found our video helpful! Thanks for watching :)
Does anyone know of any good cell culturing books they have experience with? looking to purchase one.
Cell culture the same as induction?
Hi.. Thanx for the introduction. I am working on cell lines production in Pakistan. Can you know exactly the abbreviation of SH- SY5Y stands for? These are neuroblastoma cell lines but I am looking for exact abbreviation.
Amazing intro video! Thank you!
Thanks for watching!
Thanks!
😀
Do the infected cells perform the same as their non infected counterparts? I mean, do immortalized cells maintain their function?
Hi Popa, thanks for leaving a comment! Yes, for most cases, immortalized cells hold the same phenotype and genotype to their parent tissue, meaning they retain their original function. This reason, along with their extended replicative capacity, is why immortalized cell lines are more suited for tissue culture studies than primary cells. Hope this helps!
Great video! Thanks~
We're happy you enjoyed the video, Vicky! Let us know if you have any questions or comments.
Absolutely great ! Many thanks for sharing
Thanks for watching!
hi can you please give me some examples of secondary cell lines ?
Hi Ajay, thanks for commenting and watching our video! When Primary Cells (cells which you get directly from tissue) are grown in vitro and use up all the available space or media for growth, they will stop proliferating. In order to keep the cells in the proliferation phase, we need to take some of these primary cells and grow them in a fresh dish - a process called sub-culturing. The resulting population of cells in this subculture are called secondary cell lines. So, there are as many examples of secondary cell lines as they are tissue/cell types in the body! If you want to learn more about cell culturing, we have an entire knowledge base here: goo.gl/3LR25m
Applied Biological Materials - abm Please give me any 2 example of secondary cell lines, finite cell lines and infinite cell lines if possible. just the names
Thanks so much ABM for the link! Finally, I came across into the very right website!
I love this vedio! so interesting and easy to understand Thank you
Hi Yuna! Thanks for watching and leaving a comment - glad you enjoyed our video!
wow Amazing
Thanks for watching!
So, DMEM, RPMI and F12 are defined media right? There is no need to supplement them with FBS correct?
Hi Diego! You’re correct in that DMEM, RPMI and F12 are chemically defined media. However, they are usually supplemented with FBS because FBS provides vital macromolecules, growth factors and immune molecules.
in which cell mitosis is occur adult embroynic cell or well differentiated cell????
Hi Ameer, if you want to know the difference between embryonic and adult cell with regards to their proliferation, embryonic cells proliferate rapidly and differentiate into specialized cell types which can be categorized into three kinds:
1) Terminally differentiated cells eg. cardiac muscle cells that are no longer capable of cell division. (If lost, cannot be replaced via natural repair mechanism).
2) Adult differentiated cells kept in the G0 phase and divide as required for replenishing the dead cells such as skin fibroblasts, cells lining blood vessels and epithelial cells of most internal organs, such as the liver, pancreas, kidney, etc. Most cells belong to this category and some form of injury/need to repair cells induces these cells to divide.
3) The third category is pleuripotent stem cells (less differentiated cells) which include blood cells, epithelial cells of the skin and digestive tract lining which have short life spans and must be replaced by continual cell proliferation. These divide to produce cell line lineage-specific cell type and once they commit to a specific lineage, they do not divide anymore.
Thanks❤
I love this Video
Thanks!! :D
Good video
Please give protocol
Hi Aijazali, can you clarify what protocol you are referring to?
Thanks a lot can i contact you I have some questions please !
like I need to know what raw materials are needed at this stage to produce a highly effective antigen for vaccines?
Hi Sanae,
Please contact us at technical@abmgood.com with your project details and our tech. team will further assist you ;)
Which app did u use for making the vedio??
Hi Sidra! We use Adobe After Effects to create our videos.
i have a question, so is this the same thing as cloning? im curious
Hi Sophia, thanks for leaving a comment! Cell cloning is the process of getting a population of genetically identical daughter cells from one parent cell - if you are able to start cell culture from one cell in a dish (a common strategy to isolate a single cell is to make a very high dilution), the subsequent cells will all be identical clones of this original cell. This video is discussing cell culturing in general and not specifically about cell cloning technique. Cell culture is simply a technique to take cells from an organism and grow them in vitro (an artifical environment).
Thank you very much.
No problem! :) Thanks for watching!
good basic introduction to cell culture
shan v
Wow awesome video
Thank you! Glad you enjoyed it! Please let us know if you have any questions about the material. Or, you can read more at our knowledge base: goo.gl/OtCzAJ
This is Great
Thanks for watching. Please check out other interesting videos on our channel too!
thanks a lot
You're welcome!
thank U for sharing,,....
Thanks
Thanks for watching!
Thank you for sharing~~
You are welcome! Thanks for watching.
Bruder muss morgen darüber ein Referat halten aber ich verstehe Nichts. Danke Bruder
where is the link
Perhaps this is a daft question but has anyone here cultured there owns cells in a homemade lab?
How to do that?
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RETRACTÄBLE COSMATICS ; DÜE PRÖJECTS 7:44
Great video however you talk very fast. Slow down!!
Thanks