What is PCR? Polymerase Chain Reaction | miniPCR bio™
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- Опубліковано 25 чер 2024
- We live in a moment where genetics is helping us understand more and more of the world around us, from untangling evolutionary histories to more precisely diagnosing and treating disease. But to do all of this, we need to be able to find and examine specific pieces of DNA. One of the fundamental tools that we use to do this is called PCR, or Polymerase Chain Reaction.
Find worksheets to accompany this video, classroom-ready readings, and more student-focused biotechnology videos at the tutorials page of our website: www.minipcr.com/tutorials
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Find worksheets to accompany this video, classroom-ready readings, and more student-focused biotechnology videos at the tutorials page of our website: www.minipcr.com/tutorials
Music from filmmusic.io
"Positive Advertising" by Rafael Krux
(www.orchestralis.net/)
License: CC BY
(creativecommons.org/licenses/b...)
Video copyright Amplyus LLC 2020
I want to personally thank you for the quality of these videos and both their general, universal applicability and their specificity. My labs on genotypic identification were cut short by COVID-19 and we cannot do 'wet labs' for this topic anymore. I was disappointed by the dearth of virtual labs on the entire sequence of how ID by DNA occurs (from start to finish), or that they were heavily watered down or not specific enough for sophomore/junior level microbiology courses I teach. But I feel confident I can show some of your videos and give them their sequencing data, and they will understand the procedures and importance. Please do more educational videos!
Here are some notes too.
ua-cam.com/video/boBaoIHGL6c/v-deo.html___Polymerase_Chain_Reaction
Thank you for enhancing my understanding of PCR. I was trying to put things together but was getting confused at some point. Thank very much.
Thank you so much for this amazing video and explanation 😊
This was such a helpful video! Thank you!
Thank you so much ma'am.
Awesome video.
It's very helpful for me.
By far this is the best explanation.
Simple and educating. Thank U
lucid and easily explained ...in grained in mind
I found this useful ,thank you very much .
very helpful,your manner of explaining really matches my learning abilities
. thank you
Excellently explained
Thanks for the wonderful explanation..
Thank u ma'am for this amazing video 🤩
Awesome explanation. Goldilocks would be proud: Not too much and not too little. Just right 👍
I will Ace my lab quiz because of u. Thank You!! Amazing video.
great stuff, thanks
Very well explained thanks
Thank you so much ma'am ❤ you made this much more easier for me❤😊
Thank you. Very much just saw this before and exam and you have helped so much
very well explained
The Best Explanation!
GOT PRINTED IN MY BRAIN, MAM....
TQ SOOOO MUCH FOR SUCH A NICE EXPLANATION
I love the way you explain
Thanks ma'am
Nice video! Short and understanding.
Helps so much! Thank you
Very educational!
Thank you from depth of heart ❤️
thank you very much. your explanations much helpful
STUNNING
any video about PCR types and PCR optimization?
I personally thank you for this ❤
Very nice explained 👍😊
What a video. So nice. Thank you so much
Thank you so much doctor
Thank you very much ...
This was really helpful
Excellent
THANK YOU!!
Thank you so much for this video❤
Well done
thank you❤
Beautiful explication!! You have my respects
What I don't like about using PCR for the Covid is they use this in reverse to get RNA from DNA after which they
still have to guess at what they see Because Exosomes which are abundant in humans also give off RNA and are a part of our immune system As far as virus though they cannot be sure what it is they are testing for afterwards which something this small afterwards they are simply guessing after sequencing for a virus
if they believe they find one but again are they?
@@PeachesCourage But Sars-Cov-2 is a RNA virus, so Im not sure how this process works for it :/
I'm so happy with this lecture video❤
Great video 👏
Thank you so much ma'am,, from now on your my teacher🙏 Namasthe from india
Thank you queen
I want to thank you for this video it's interesting and educative indeed
Thanks you 😌❤️
Thank you so much!!
We hope it was helpful!
It reminds me of a sewing machine. But fascinating. I wonder can we see it in action working under a microscope?
Perfect
I love u. finally I understood!
So glad it was helpful!
Is there a tempurature requirement for extension?
The video was amazing!!!
yeah
Great video. Im at novice in behavioural genetics, and still struggle to understand how the sequencing is helpful? Can you really from having just a few nucleotides to start with, work out the rest of the whole sequence through this PCR process?
If I give you A, C, A, T, can you with the help of these enzymes figure out the rest?
For the amplification to be successful do the primers only need to connect their 5' end or both their 5' and their 3' end?
The 3' end is critical for amplification, since that's where the DNA chain gets extended.
Thank you, this was well explained.
How do we get the primers and dntp s to run
QUESTION: how can someone generate the primers?
I'm watching this for a microbiology class wondering if in fact some people are just better able to understand this stuff than others. I'm not able to retain this information because it just doesn't make any sense to me. It's discouraging and it's by no means the fault of the person creating the video. The video was just fine. I get really down on myself about this stuff though. Things would be a lot easier if I could actually understand it better.
Oh!!!! I Mercy for you
I have a question, why can't helicase enzyme be used to unzip the strands?
Enzymes are difficult/expensive to produce, and most would become denatured at the temperatures used in the thermal cycler. Heating it to 94 is cheap, easy, and dependable!
jesus christ you're amazing
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This video is better, but I’ll just use BioRad equipment for now xD
Your body, your choice
wait I'm confused, if the optimal working temperature of polymerase is 72 degrees (4:41), then why would it denature at "these high temperatures" (4:56)?
72 degrees is the optimal working temperature for Taq DNA polymerase, the one found in hot springs. The polymerases that would denature at 72 degrees are the ones from humans or typical bacteria. She mentions that a few seconds before the 4:56 mark
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So ...... as I understand it. In case that no DNA piece matches the primer no multiplication will happen?
Does that mean that fx. PCR-test fpr covid-19 does not produce false positives?
In a well-designed PCR, the primers will be specific to the sequence that is being amplified. This makes false positives from a PCR test very unlikely.
where can i get that t shirt?
minipcr.com/store
its easy to undrestand.
the video i found about PCR
🌹
Then the difference between PCR and DNA replication???
PCR is a method of amplifying and replicating DNA in vitro! It's similar to the process that happens in living cells, but reproduced in a controlled lab setting.
How does one procure spare nucleotides?
Plenty of coming sell nucleotides. We get ours from Promega.
I need a long term of download by free facilities*
In silico: ua-cam.com/video/BkTRYMjyatA/v-deo.html
ua-cam.com/video/nEhKEchZO8w/v-deo.html
is it possibile that a covid19 pcr test is sensible to other virus ? like a false positive ?
pcr is not a test, its a production (multiplication) process, it does not tell you anything about the nature of the entity that you are making copies of.
@@jcezary scuse me... all the government use pcr to tell people that are infected or not these days. is that possible that you use a pcr to find a covid 19 genetic target but the technnique amplify another target ? that's my question. are the tests precise ?
@@jpcapobianco1979 of course it is, the process does not tell you what the target is, it simply multiplies the target molecule.
@@jcezary scuse me ... how does it work ? if i know the target of covid... and i develop a primer for covid 19, can the primer attach to the normal influenza virus and amply another dna sample ?.... the question is are the PRIMER specific only for covid ? or there's a risk that i've ifluenza and i test positive for covid ?
@@jpcapobianco1979 I'm not a pcr specialist but how do you develop a specific primer for a virus that has never been isolated?
Wow, when physicists talk about forces, momentum, acceleration etc they always drop Newton's name somewhere along during their presentation. Engineers drop names like Faraday, Watt, Babbage etc.
Not once throughout this presentation did the learned doctor mention Kary Mullis, whose body, at the time of her upload (Feb 5, 2020), was barely decomposed. The poor man was screwed over by Cetus, so often got banned from speaking to make some money, and now his lifetime's work is being talked about without even a mention of his name as its inventor. That's quite disrespectful, in my opinion.
Mullis did indeed invent it, and a great deal of work would not have been possible without his work. The trouble is that Mullis later in his life started taking some decidedly non-scientific views. He's entitled to whatever he wants to think of course, but it did mean that he fell out of favor in many scientific circles.
Quem entende essa língua aí me fala por favor curte aí porque eu não sei de nada disso
V-scene science is tobacco science. Do your own research.
the music in the background is really loud and very distracting especially to some autistic people like me
Are you American?
She’s from Camaroon
@@Underwearscares are you her friend?
This video is strangely oversimplified to the point of being almost incorrect. It completely glosses over crucial points about short and long fragments.
NEET ASPIRANTS ATTENDENCE 👇
We don't need the music. It's a distraction and it trivialises the speaker and the video, making it seem a light and fluffy trivia piece --- or an ad. Perhaps do NOT involve the marketing department in info videos.
Sorry you didn't appreciate the soundrack. That was not a marketing choice, just happen to like it.
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