Liquid Culture: Better, Faster, Stronger
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- Опубліковано 7 бер 2021
- If you want your liquid culture thick like ours, give it a nutritional boost with Peptone and stir it often. Ever since we’ve started using this method our liquid culture has been growing in leaps and bounds. We tried several different methods, but nothing worked as well for us as Peptone.
Check out our library of liquid culture:
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I am a beginner mushroom farmer in Washington state. I have my first set of bags in the incubation room and I'm almost done getting the fruiting room ready. I just wanted to thank you Andrew for taking so much of your time to make all of your videos for all of us. I am learning so much from you I am truly blessed by your example. I'm only growing mushrooms to eat, and I am stoked to have this new project to keep me busy. This makes retirement a real joy. Thanks again. David
Great video! I am actually the export manager for Yuantide Peptone and your video helps us decide to release the consumer version so thank you for that! Peptone is commonly used in fermentation industry acting as a source for protein. Based on our experience from that practice, you can also try to add yeast extract (not yeast) into the mix and see if there's any benefit. Peptone from different material sources can also make a difference (think of it like eating different types of meat), and our client often sees greater benefits from mixing both plant-based and animal-based peptone together (so soy peptone and bone/fish/casein peptone).
I was thinking about buying your product online. I was wondering do you know if yeast extract would be faster for mycelium? I see you have both products and was wondering.
@@andrewbarnett2761 Actually, our yeast extract product is still in transit, but it should be ready in the next few weeks. Meanwhile, you can purchase yeast extract from other companies. Yeast extract is essentially yeast without its outer cell layer, which leads to a higher concentration of nitrogen or protein. We haven't tried it on mycelium, so the main question would be whether the fungi prefer a lower concentration of sugar and a higher concentration of protein from yeast or not. Most bio-pharma plants here have chosen a combination of yeast extract and peptone (because the combination provides a better profile of amino acids), so I hope this provides you with some direction :)
Do all of the peptones smell like rot? I’ve used hiveg in my LC but it makes it cloudy and stinky.
Hi, in terms of smell yes. Peptone is basically hydrolyzed protein, and those smaller molecule tends to smell rot as you said. However, it shouldn't be cloudy at the concentration this video is using. I assume hiveg is a product by HiMedia so I can't vouch for their products, but our peptone don't typically appear cloudy at such low concentration. @@TheOffCycle
You are such a blessing to so many. Thank you.
Thank you for sharing! I have Paul's books but haven't worked all the way through them yet. You're inspiring me to get to reading!
I use brewers yeast (nutritional) and it made massive clouds of super fast cultivating culture.
You’re awesome dude! Love you and your videos. Thank you so much for all you do. SO helpful!!!!!!!!!!
Comment for the algorithm gods. Thank you for the Educational content. Love from Canada 🇨🇦:)
I read about peptone in Radical Mycology in one of the recipes, and have also found it to be AMAZING. I find adding peptone to Lion's Mane liquid culture is especially a game changer as Lion's Mane in my experience has a harder time growing in LC than other species. Thanks for the video! I will have to try out that laser tip.
Thanks for sharing Master Andrew !
Love this stuff. Great recommendation
Thanks again for the update and information 😀
Thanks for the your kindly information. Its useful for new learners 🙏🙏🙏
Gonna try the peptone, thanks brotha!!!!
I just realized that you can use air pressure to dispense directly from your LC jars. Connect an air hose to the air exhaust port filter, and "run it in reverse" to push out the liquid...
Almost forgot to use the affiliate link..... I'm sure it's a small percent, but I know I would want it. Thanks for the video!
I am so happy with the results when using peptone. I can't thank you enough for the advice.
Great info! Thanks 😉
Bro, You have hit the pandora box on LC. I did some Isolate and since using Peptone it has only taken 2 days (unheard of for me/lol) for the Mycelium to grow.. Thank you, thank you, thank you, you have made my life so much more complete!! Mush Luv 🍄💙
This stuff is freaking amazing
Top quality info!
Thanks for the tip
You are the man for this Video !!
Your channel is highly underrated. I've been into mycology for months and youtube has only finally suggested your channel. So silly.
You are an excellent presenter. Going through your agar stuff now. Thank you for putting these out there
Unfortunately, when you haven't been around long enough, it's hard to tell who to listen to and who not to. Adding motorbike sounds to your video doesn't make it less bullshit. Guy shows no proof, and makes bullshit claims about using a Lazer pointer to look for contams is the dead give away, the guy is living in his own privates confirmation bias lala land. Only way to safety test LC is on agar.
@@dracolusus Why are you so agitated friend? He is just sharing practical experience. This video is useful to me.
Draco I agree about the laser, my guess is he misinterpreted paul stammets when he spoke about using laser particle counters to check sterility of newly made flowhoods
Thanks Paul!
@@dracolusus Lol, never said it shows contamination. I said I use it to help look at the mycelium, if the LC is cloudy, that is usually a dead give away that it's contaminated.
Thanks for the laser tip! 🐬
I added pepton. It works great. Thanks
Thank you so much.
Thanks great work 🇨🇦
Going too be putting peptone into my very first LCs tomorrow! Very excited! Will give you an update!
Update!
Update!
I purchased some pink oysters from y’all! Can’t wait to grow em
Good tip!!
appreciate the recipe!!
Thank you
Thanks!
Thank you for sharing 🙏🍄🤙
I've been putting peptone in my agar. This stuff is awesome!!
Do you prefer this over yeast extract?
Thank you for sharing all your secrets with us!! 🍄❤
I got another tip for fast and full growth too. 0.1% agar added to LC mix.
I'll have to try that. Why does it speed up the growth do you think?
Idk for sure, just know it works, I guess it's just easier for mycelium to catch on to agar than just water, but the other good side to it is it makes pooling less of an issue when using LC in a grain jar
Thanks for this tip. I really appreciate it.
Just spoke with Brendan with Foggy Dew Fungi in New Castle and we were speaking about how we’re excited to start a new project with 5 Gallon Buckets and cultivate some Oysters species, on a beautiful farm named Laughing Duck Farm. Really excited I have some amazing role models like you and Brendan. Can’t wait to provide food for this blessed home of ours. Good luck and wish you the most success! 🤗
New castle in California? I know Brendan! He's awesome!
Hey your videos are awesome!!! I love watching um.. any way I had a question!! I'm new to this... Can I use callagen peptides for peptone??? Peptone is kinda hard to get!!!!
So funny I just ordered peptone today before you posted this. Heard in a video about organic cordyceps growing that they would have used peptones but could not because of the organic status. I figured since I am not growing organic I could benefit from it. Plan on using in my multipurpose LC, and in nutrient mix/brown rice for cordyceps.
There are soy based peptones, too. In fact the NIH study found plant based peptone to be superior growth.
Awesome video man keep it up....I hope I get the opportunity of meeting you some day
Hey, love your video and I'm excited to try this out.
I'm new to growing mushrooms but I have some peptone and some spores on the way.
Now I know this video is for a liquid culture which is different to just spores but how can I use my peptone to my advantage with just spores?
Add it to your agar recipe
Good video
TTT Damn you, no I need to order peptone. Been wanting to use it in my agar as I hear its awesome. @mossy do you use it in your agar too? Peace
Trying peptone with a lc . Followed your advice and used 1 g . Will follow up on how thick my mycelium gets 😅
Thanks for the video. I always wonder how you stir those 200 jars of LC each day? Must take hours even with 10 stirrers!
I bought peptone and have been using it for the last couple months. It's funny, you talked about myc forming a mat on the surface on the LC and I almost never had a problem with that just stirring the jars by hand. I started using peptone, and my jars became cloudy, started to look way different to the point I thought they had contams. Also started seeing myc grow vigorous mats on the surface - eventually I realized this was all because of the peptone. I have noticed way faster colonization times from the jars that have peptone and stir bars compared to without them. It's pretty insane.
What’s your recipe man?
@@JayJay-bd6ti I've been experimenting a lot.Traditionally I've used molasses, now I add a small amount of peptone to that. Visually, I add enough to darken the water but keep from making it cloudy. I feel like adding too much sugar can be bad but I don't know about peptone.
You using clones or MS?
I love peptone been using it fr 14 years
I used to use pepton in a agar andit cases oiled bacteria
Interesting. Do you add peptone to anything else like your grain spawn?
i remember when peptone was 14 bucks. its almost 30 now but still worth it
For the Shroom supply premix, do you use the same recipe as your DIY mix?
500ml water, 20 grams premix, 1 gram peptone?
Hi Andrew! Love your videos. I was reading to stay around 4% sugars, but see that with your premix it's a little higher. Have you run into any troubles with a sweeter mix?
I have to reset my cultures more often when running this rich, but that's it really.
You also have to be careful when autoclaving to high or too long, as you can caramelize the sugar. Then it's no good.
Man you know some stuff.
Thanks for the tips man- does the species of mushroom matter for peptone? are is it usually good for all mycelium
I'm speaking for commonly grown white rot fungus here.
BROTHER YOU ARE AMAZING ,I HAVE LEARNED SO MUCH FROM YOU ,IT IS SO REFRESHING TO HAVE A FRIEND THAT IS HELPING US SUCCEED ,INSTEAD OF TRYING TO DECEIVE US WITH BAD INFO BY LEAVING IMPORTANT INFO OUT.I BOUGHT THE PAUL STAMETS BOOK THAT YOU USE AND PLAN ON ORDERING YOUR LC MIX.TY FOR YOUR FRIENDSHIP. HAVE YOU THOUGHT OF OFFERING ,SPEND A DAY WITH ANDREW ON HIS FARM TO LEARN HOW TO GROW FOR A FEE? YOU COULD EVEN DO GROUPS. JUST A THOUGHT MY FRIEND .
Is bacteriological Peptone and Soy Peptone the same thing in regards to liquid cultures and substrate nutrient enhancement or is one better than the other for mushroom cultivation?
I watch this video like twice a year dude (I could write the recipe down but I like the full explanation, Weird I know)
Hey what do you use for agitators in your LC jars?
$14 you say for peptone!? I wish! Up here in canada, minimum $40 Plus shipping cost. We get hosed hard here :( Good stuff as always though.
Is buffered peptone a bad choice? I know it helps with contam but would it effect mycelium growth?
Do you use peptone in with the Shroom Supply premix you mentioned in another video?
Great guy and great production! We have the same last name maybe we're related 🤟🏽
I've gotta say, you should be careful with which lasers you use to check LC!!! A lot of internet green lasers are insanely overpowered and often outright lie on the sticker. A $10 green laser could be easily be 80mW+ which are *powerful enough to vaporize your retina faster than you can blink* should an errant or reflected beam strike your eye in the wrong way.
Just make sure you use with extreme caution. Green lasers are actually made by shining IR laser diodes through a crystal that modifies the beam's wavelength. These are not efficient either, so even laser safety goggles rated for just green light could let IR right through, which is invisible but just as dangerous as visible light.
That being said if you are careful and only shine it directly into a body like in the video it gets dispersed rapidly. This reduces intensity of the light but is not guaranteed to be eye safe either.
I know this video is old, but I really appreciate the tips! I just started collecting strains and making LCs. But I have a question, how do you dispose of your contaminated LC’s?? Do you toss the whole jar? Save the jar?
Without opening, run the contaminated jar through the pressure cooker to kill the contaminant, then you can open the jar and dispose of the contents and wash the jar normally. The whole culture is definitely lost though, if that's what you meant, barring re-isolating the sample using agar techniques.
Thanks guy, do you find that this helps with infection, or you do you allay that with sterilization
when do you add the peptone?
WHat is the best formulation for
MALT
DEXTROSE??
Has anyone found any other places to order the bacteriological peptone? They're sold out on Amazon through this link, and there's a long wait time from other suppliers.
I've been doing just a bit of Honey. Would you recommend just adding a gram of peptone to 20grams honey~500ml h2o?
What about using peptone with agar plates?
will soya peptone work?
Do you have to use bacteriological peptone? Or can you use hi veg peptone?
Could you add peptone to choir and get a better yield?
can I use the peptone from the health food store like gnc?
How long does the LC last for
Does this work for agar too?
Could the peptone be used in agar?
Is it normal for it to get foamy like beer when stirred, if not I need to know what I'm doing wrong
Is there a video on how to make agar with peptone????
used LME and peptone in some LC and let it set for a few days after PC. when I went to inoculate I noticed a lot of stuff floating around. Is this normal? Doesn't look cloudy, but definitely a lot of stuff in there. I figured the nutrients would remain as sediment.
Is there a difference between soy peptone and bacteriological peptone? You also NEED some laser glasses if you are playing around with them like that.
I ordered the peptone and when I got it here I found out it’s the “hi-Veg” peptone vegetable protein version. Will it work the same or the animal one is better?
Murashige and Skoog baby
What size syringes do you use to innoculate those monster mycelium?
What is lc premix?
can you give me information about it so i can buy it please
No Vegemite or a replacement?
Is that mushroom agar powder with soy peptone?
Wats ITS the LC premix plis on latino América thans bless for you
This man reads my mind.
Have you tried adding peptone to bulk substrate? If so any pros or cons?
I'm about to put a teaspoon in a bag here soon also
From what I hear peptone is not allowed in organic mushroom cultivation, can anyone elaborate? I can't find much information on the topic.
Will peptone from meat work
I'm thinking since the peptone (a source of nitrogen) works so well maybe a shot of Bragg's liquid aminos (which may be purchased in the grocery store) will be a good substitute.
Did you end up doing this, seems like worth trying.
???
Are you able to use this recipe for agar?
can you sterilize the peptone with lc? i assume not
They said it's not good to shake the jar rather use the magnet to stir
Hi Andrew! Thank you for the great tip! Would you use peptone in your spawn? I recently started adding a tablespoon of Peptone after my grains are soaked and drained (I guess I can just soak with peptone just like gypsum). I would like to hear your thoughts!
Thanks!
I use to add it to my bulk sub. I got go results off that.
@@mycologycorner did you mean good results or no results?
Yea good results
@@mycologycorner how much do you add
@@Andrew-co6ye much 2 tablespoons. But after switching to whole oats I don’t have to supplement my grain spawn. I processed 185 5 pound grain spawn bags last week. Whole oats and hard red wheat.
Thanks for sharing this. Have a great day and best wishes from Austria 🇦🇹[the country with the exploding trees, not the one with kangaroos 😉]
Where Hitler came from is how people not from Austria know it as
@@drfeelgood1992 where do you come from?
Why not add nutritional yeast also with peptone?