MTT Assays: Part 2 - Protocol

[@ifechukwuajoku5037]

Very helpful

[@atuletenijessica2061]

Thank you

[@thebiobuddy9595]

Thnku❤

[@MoticusFervor]

I was instructed to read inteference at 690nm. Any thoughts on that?

[@josuecaro4592]

A reference wavelength of 630 nm is sometimes used, but not necessary for most assay conditions.

[@SadiaKhan-l4w]

I read on 490. Is it a problem?

[@josuecaro4592]

Riss TL, Moravec RA, Niles AL, et al. Cell Viability Assays. 2013 May 1 [Updated 2016 Jul 1]. In: Markossian S, Grossman A, Arkin M, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-. Available from: www.ncbi.nlm.nih.gov/books/NBK144065/