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you all probably dont care at all but does anyone know a method to get back into an instagram account? I stupidly lost my account password. I would appreciate any tricks you can give me
I have one question Ma'am. When scraping and transferring the E. coli bacteria from the E. coli starter plate to the (+)BLU and the (-)BLU tubes, why is it important that you not scrape up any of the agar in the process?
In general, it is best practice to scrape agar plates gently because the agar can easily be disturbed while collecting your bacterial cells. The inclusion of agar (or any components that may be present within the agar such as X-gal) in your cellular suspension may adversely affect downstream processes or experiments.
Addgene does not regularly monitor comments posted here, so we may not see your question immediately. We’d be happy to answer any questions sent to help@addgene.org as soon as possible. Please include the name of the video along with any questions so our support team can help. Thanks!
A very clear and helpful explanation!
Thank you very much!
nice video i learn so much
Is it nutrient agar or mha?
Do you have a video on Asceptic Technique?
Not yet, but that sounds like it would be a great topic for a video. Stay tuned!
SA
you all probably dont care at all but does anyone know a method to get back into an instagram account?
I stupidly lost my account password. I would appreciate any tricks you can give me
@Kayson Canaan Instablaster :)
@@omerhayri999 AS gardaş
Mam..... how does simultaneous exposure of the cell to ice and hot water bath.... increase the chances of transformation?
The precise mechanism is not known. But the theory is they the cell walls open up and become "leaky" allowing transformation to happen
Thank you mam
I have one question Ma'am. When scraping and transferring the E. coli bacteria from the E. coli starter plate to the (+)BLU and the (-)BLU tubes, why is it important that you not scrape up any of the agar in the process?
In general, it is best practice to scrape agar plates gently because the agar can easily be disturbed while collecting your bacterial cells. The inclusion of agar (or any components that may be present within the agar such as X-gal) in your cellular suspension may adversely affect downstream processes or experiments.
nice vid 👍