So so helpful, thanks for all of these videos. I work in blood transfusion and wanted to learn more about Micro, explained clearly and very informative. Can imagine this guy is an excellent trainer for the staff he works with.
Its really fun when you can correlate your knowlege of micro you absorbed in your classes. Really makes you feel proud that you know your basics well. I am happy Alhamdulillah
4:05 - Actually several Haemophilus species DO grow on both chocolate and blood agar. The chocolate vs blood comparison is typically used to identify Haemophilus influenzae which is pathogenic and can not grow on blood agar alone... but CAN grow in the beta-hemolysis rings caused by other bacteria (like S. aureus). Haemophilus parafluenzae however is non-pathogenic (usually) and it can grow on both plates. The two Haemophilus species are impossible to distinguish otherwise under a microscope/stained-slide. This isn't the only way chocolate plates are used, but its a key reason all throat swabs get chocolate plating in our lab. :)
He probably wasn’t supplementing with CO2. H influenzae won’t grow on chocolate with regular ambient air. Requires CO2. Common mistake. Temperature is important but for most human pathogens- they like added 5% CO2.
Great promotional video for microbiology! Love to see stuff like this to get people interested in all things biomedical. I'm hoping to inspire as well with my own channel!
Best career in the world. If you can get an internship in a lab, I encourage you to do it! Best way to find out if lab work is for you. It’s not a typical job. You will work holidays and weekends. Your life revolves around those microbes. I love it but it’s not for everyone. Best way to see if it’s for you is to actually do it. Best of luck to you in finding your path.
@simona14033 I just finished my bachelors in microbiology. I can honestly say the curriculum isnt too bad. You're pretty much just going to learn about the different kinds of bacterias(gram negative and gram positives), structures of bacterias, and pathways regarding protein synthesis, and transport. In your lab courses you're going to learn about aseptic technique(keeping everything sterile),streaking, and a lot of different test procedures. It may seem intimidating right now, but when you actually dive into your microbio classes it can be really fun. On the plus side, having a microbiology degree means you can work anywhere that deals with bacteria: food industry, pharmaceutical, medical, water, etc. The degree itself is very flexible compared to something like a human bio degree.
This is easy; simple; direct; Try role of inter-colony signalling pheromones in Super Colonies for S. Invictus → Latin for invincible: Your helmet would be funny to them with ½-million specimens injecting Necrotoxins in Unison ▬ then if that is not enough try a species that just eats them for lunch - which then can be a carrier for Nematodes; Which then produce a subjective perception of having a *Tapeworm*
microbiologists generally don't wear gloves because you work with a flame to transfer microbes. Pretty much personal preference, I don't wear them because I don't want them to get melted to my hand. I do have an issue with these plates being open, they could be easily contaminated, you should only open them when necessary.
At 5:03, it looked like Proteus mirabilis growing on the blood agar plate to me. And a few seconds later, he said CNA was used to inhibit Proteus...hahahha.....I'm so proud of myself. LOL. Very good at explaining the differences of plates. And yeahhh...nice hair too.
Caroline you are right! Best career ever❤️. I’ve been doing microbiology research for 25+ years and go to work with a smile every day. Very rewarding career. I encourage you to look into it if microbiology fascinates you. It captured my heart with a single Nova video on Brazilian Puperic fever. Set my goal to work where I do now and ironically ended up working with the Brazilian researcher from that very Nova video. Small world (microbiology pun intended 😊)
hi,you said agar is required to support the nutritional requirements and it has all the amino acids while we know that agar is just the solidifying agent to give the medium a solid texture and as far as it is known agar itself does not bear any growth factors or anything that might support the bacterial growth.If agar is the nutrition, shouldn't we find it dissolved or degraded because of the consumption of the bacterial culture as some of the bacterial culture does that to gelatin? thank you.
The base is agar, but when you make it into an agar, a premix of other ingredients like amino acid, lactose, glucose or other excipients have already been added. This is known as media. When making media, that is agar with other ingredients, simply boil water and add the premix powder and bring to boil. After which, the agar is put in bottles, autoclaved so that it is sterlized and cooled.
Only the fastest growing bacteria only take 6-8 hours to reproduce/fill a culture. Some slow growing bacteria such as mycobacterium leprosy take up to 14 days to even double in number
I'm a microbiologist the photo in the first shot is an example of how not to streak out a culture plate. The whole idea is to get separated completely isolated colonies of the various things in the sample so you can pick a colony that is totally pure. I still think it's abab idea to have untrained people doing this. It can be very dangerous if you don't know what you're doing. Also in your garage or whatever you don't have an autoclave. How could someone trying this safety dispose of the culture even if it's just a culture done on a bit of dirt from your backyard ( botulism is in all natural dirt and even a tiny amount of toxin in a culture can kill) .
Nope. They grow via log exponential growth. Each cell splits into two. If you are new to this, draw it out on a piece of paper. That will help cement the difference in your head.
I'm a baccalaureate student and i am really interested in microbiology. Do i need to do chemistry and maths for this subject because i am also studying those subjects but it seems from the video that they are not that important. i also need to know about payment, if you get paid enough? because i was thinking of marine biology and ive heard that get's more money? plz someone answer my questions :)
Wearing gloves in the microbiology lab is only a recent innovation. Unless the skin is injured or not healthy frequent hand washing was the only necessary requirement as skin is an impermeable barrier. And besides, everybody's hands have normal skin flora anyway which includes numerous staph species including the well know pathogen S. aureus. It's been argued that applying a glove over the skin causes the bacteria normally present there to multiply under those warm and humid conditions.
"Ay-gahr"????? In my 25+ years of lab work, I have NEVER heard anyone pronounce agar that way. It sounds like a damn superhero. It's "Aw-ger" (with one of those funny, upside-down/backward "e")
We pronounce it as Ay-gahr in Canada as well. Aw-ger sounds very werid to us, but we understand what you are talking about. Just as Ay-gahr sounds werid to you.
So so helpful, thanks for all of these videos. I work in blood transfusion and wanted to learn more about Micro, explained clearly and very informative. Can imagine this guy is an excellent trainer for the staff he works with.
love love love this. good for beginners that don't know anything about microbiology. its coming around full circle now.
I love microbiology. Its so interesting 👍
Very good teacher. I wish I had him when I was in Micro.
He explains super well
Its really fun when you can correlate your knowlege of micro you absorbed in your classes. Really makes you feel proud that you know your basics well. I am happy Alhamdulillah
Al hamdoulillah I'm very happy for you
Business in the front. Party in the back.
☠️
🤣🤣🤣🤣🎉🎉🎉😭😭😭
4:05 - Actually several Haemophilus species DO grow on both chocolate and blood agar.
The chocolate vs blood comparison is typically used to identify Haemophilus influenzae which is pathogenic and can not grow on blood agar alone... but CAN grow in the beta-hemolysis rings caused by other bacteria (like S. aureus).
Haemophilus parafluenzae however is non-pathogenic (usually) and it can grow on both plates. The two Haemophilus species are impossible to distinguish otherwise under a microscope/stained-slide.
This isn't the only way chocolate plates are used, but its a key reason all throat swabs get chocolate plating in our lab. :)
He probably wasn’t supplementing with CO2. H influenzae won’t grow on chocolate with regular ambient air. Requires CO2. Common mistake. Temperature is important but for most human pathogens- they like added 5% CO2.
Oh shit , and i love chocolate so much .
So bacteria have been growing on it all this time
@@s101077, are you joking?
@@dcwood2137 ye
I love this video clip. I show it to my classes often.
Great promotional video for microbiology! Love to see stuff like this to get people interested in all things biomedical. I'm hoping to inspire as well with my own channel!
im majoring in microbiology and here i am thinking what the hell i got my self into
same, Im uncertain... how are u holding on?
If it is not your passion, don't do it.
Best career in the world. If you can get an internship in a lab, I encourage you to do it! Best way to find out if lab work is for you. It’s not a typical job. You will work holidays and weekends. Your life revolves around those microbes. I love it but it’s not for everyone. Best way to see if it’s for you is to actually do it. Best of luck to you in finding your path.
@simona14033 I just finished my bachelors in microbiology. I can honestly say the curriculum isnt too bad. You're pretty much just going to learn about the different kinds of bacterias(gram negative and gram positives), structures of bacterias, and pathways regarding protein synthesis, and transport.
In your lab courses you're going to learn about aseptic technique(keeping everything sterile),streaking, and a lot of different test procedures.
It may seem intimidating right now, but when you actually dive into your microbio classes it can be really fun.
On the plus side, having a microbiology degree means you can work anywhere that deals with bacteria: food industry, pharmaceutical, medical, water, etc. The degree itself is very flexible compared to something like a human bio degree.
This is easy; simple; direct;
Try role of inter-colony signalling pheromones in Super Colonies for S. Invictus → Latin for invincible: Your helmet would be funny to them with ½-million specimens injecting Necrotoxins in Unison ▬ then if that is not enough try a species that just eats them for lunch - which then can be a carrier for Nematodes; Which then produce a subjective perception of having a *Tapeworm*
That's a epic hair style.
lol
this video looks like it's from the 90s, lol
true
I knew that was coming.
@@Beachandpool This video is 10 years old dude
What kind of microorganisms inhabit a mullet?
Lol!
Scotty kilmer ?
this was great I just hated the person recording who didn't zoom in on the agar plates most of the time. -___-
Who else noticed the business in front and party in back the second this dude came on screen??
This guy is straight out of 1985.
in addition to what George said, it also makes us aware of what we are touching and more careful, thus less contamination.
i got informed about different incubaters for the first time, thanks alot
the song used in the titlesections is AWESOME
Very good👍
microbiologists generally don't wear gloves because you work with a flame to transfer microbes. Pretty much personal preference, I don't wear them because I don't want them to get melted to my hand.
I do have an issue with these plates being open, they could be easily contaminated, you should only open them when necessary.
Dude’s hair is giving me LIFE 😂😂😂😂😂😂😂😂😂
I enjoyed your lesson very much, wish i were close to you always, bht i appreciate much,
Thanks a lot. I am a beginner and it was so essential for me.
I work at a microbiology lab and im only in highschool. I just derp out there idk what tf is going on
Pretty good to explain the basics to non Medical staff and beginners
I really like to make it to med school but we need at least 96% of points.
So I'm just trying to accept the fact that I might be a microbiologist
Very informative, Ty. Btw Keep the David Bowie haircut :)
Are agar plates the same thing as Petri dishes
Yes. It's a petri dish with agar in it.
Because microbiologists frequently use Bunsen burners, wearing gloves becomes a hazard.
So, it's "better" to wash the hand before and after the labs, than wearing gloves? Although one does not limit the other...
Thanks for the very nice examples and explanations.
thanks a lot for your nice video, i am a LAB SCIENTIST STUDENT . i think that 40-42 centigrade degree is specific to CAMPYLOBACTER JEJUNI.
At 5:03, it looked like Proteus mirabilis growing on the blood agar plate to me. And a few seconds later, he said CNA was used to inhibit Proteus...hahahha.....I'm so proud of myself. LOL.
Very good at explaining the differences of plates. And yeahhh...nice hair too.
Extremely informative and also fun to watch. eggo approves
microbiology looks fun and happy field. :)
Caroline you are right! Best career ever❤️. I’ve been doing microbiology research for 25+ years and go to work with a smile every day. Very rewarding career. I encourage you to look into it if microbiology fascinates you. It captured my heart with a single Nova video on Brazilian Puperic fever. Set my goal to work where I do now and ironically ended up working with the Brazilian researcher from that very Nova video. Small world (microbiology pun intended 😊)
Wow. That’s impressive. Are you still in the microbiology field?
very informative, thank you
hi,you said agar is required to support the nutritional requirements and it has all the amino acids while we know that agar is just the solidifying agent to give the medium a solid texture and as far as it is known agar itself does not bear any growth factors or anything that might support the bacterial growth.If agar is the nutrition, shouldn't we find it dissolved or degraded because of the consumption of the bacterial culture as some of the bacterial culture does that to gelatin?
thank you.
The base is agar, but when you make it into an agar, a premix of other ingredients like amino acid, lactose, glucose or other excipients have already been added. This is known as media. When making media, that is agar with other ingredients, simply boil water and add the premix powder and bring to boil. After which, the agar is put in bottles, autoclaved so that it is sterlized and cooled.
Only the fastest growing bacteria only take 6-8 hours to reproduce/fill a culture. Some slow growing bacteria such as mycobacterium leprosy take up to 14 days to even double in number
Thank you sir and please explain selective media
love the intro music, gonna have to put that on the ipod so i can listen to it in the car, or on the elevator
It is very helpful & knowledge able.
Who is the man? So handsome one!!
5:22 I think he wanted to say selective plate and not differential. The correct answer is selective.
I knew he was going to say it was the campylobactor that was grown in the 42 degree incubator, woot.
This guy is an awesome teacher, but the cameraman didn't zoom in on the agar plates most of the time :(
Very interesting , thank you!
sir ,can you provide pharmaceutical testing procedures.
I'm a microbiologist the photo in the first shot is an example of how not to streak out a culture plate. The whole idea is to get separated completely isolated colonies of the various things in the sample so you can pick a colony that is totally pure. I still think it's abab idea to have untrained people doing this. It can be very dangerous if you don't know what you're doing. Also in your garage or whatever you don't have an autoclave. How could someone trying this safety dispose of the culture even if it's just a culture done on a bit of dirt from your backyard ( botulism is in all natural dirt and even a tiny amount of toxin in a culture can kill) .
شكرا لك دكتور thank you dctor
Hello, thank you so mu . You are soooooo gooood !!!
Good introduce of lab...
#MedicoFem
Wouldn't 4 bacteria divide into 8?
Very interesting and helpfull videos.Thanks!
Thank you,that was usefull for me💜
I am a microbiologist. If any students from microbiology biotechnology or pharmacy dep. needs any type of help , feel free to ask any time.👍👍
2-4 4-16 correct me if I'm wrong but wouldn't it be 4-8 before 16?
It's EXPONENTIAL growth. Very simple high school math
+Miss Loubi that's why bacteria spread like wild fire 2-4-16-256....
PsychoticAnarchist69 exponential growth is number of bacteria to the power of 2
Nope. They grow via log exponential growth. Each cell splits into two. If you are new to this, draw it out on a piece of paper. That will help cement the difference in your head.
I miss this work.since three years our section of microbiolgy is closed because
financial broplem of the hospital.great work.
pretty useful for beginners
I'm a baccalaureate student and i am really interested in microbiology. Do i need to do chemistry and maths for this subject because i am also studying those subjects but it seems from the video that they are not that important. i also need to know about payment, if you get paid enough? because i was thinking of marine biology and ive heard that get's more money? plz someone answer my questions :)
Basic information .. Like it
u're not wearing the sterile gloves »don't u fear of getting lab-acquired infection ?
Anyhow.. Thanks doctor
Looks like a great lab.
Omg 😮 14years before ❤😮
When using the blood agar and Chocolate agar
i have a questn pls "btw microbiology and medical lab which one is the best?
Microbiology is a section WITHIN the Medical Lab.
Is it celsius or farenheight
some great inforamtion here thanks
So this was 11 years ago.
you can tell hes been doing this for a long time cuz he no longer uses gloves
Thank you mate. but Close the lab coat.
Wearing gloves in the microbiology lab is only a recent innovation. Unless the skin is injured or not healthy frequent hand washing was the only necessary requirement as skin is an impermeable barrier. And besides, everybody's hands have normal skin flora anyway which includes numerous staph species including the well know pathogen S. aureus. It's been argued that applying a glove over the skin causes the bacteria normally present there to multiply under those warm and humid conditions.
Whats your salary?
Perhaps you can get an estimate in one of my videos :)
excellent..!!!
what is bacteria can grow acid?
Ian completed diploma MAY from india
Is this course is very difficult . I'm also going to take admission in few days pls guides m b india se hu🇮🇪
what about using some gloves? is the most basic principle
"Ay-gahr"????? In my 25+ years of lab work, I have NEVER heard anyone pronounce agar that way. It sounds like a damn superhero.
It's "Aw-ger" (with one of those funny, upside-down/backward "e")
We pronounce it as Ay-gahr in Canada as well. Aw-ger sounds very werid to us, but we understand what you are talking about. Just as Ay-gahr sounds werid to you.
this is great
Super
CNA is selective, not differential. The colonies that grow are gram positive, they don't look different.
"4 into 16"
Thank you very informative
If anyone spread a plate as slopily as in the intro in my lab, they woud be taken aside and retrained.
You talked about a lot of pathogens. I'm a biologist, I do research in biochemistry. Why don't you wear gloves touching that plates? Is not harmfull?
thank you sir !!!!!
man just contaminated the fuck out of everything lol
Hello from 2021!
Thanks sir
very helpful, thanks!
very helpful thanks
THIS MANS HAIR
Dude...what happened to universal precautions? Hell...where's the gloves at bro?
good lecture
I just wish I had watched this video earlier. Wouldn't have struggled getting my bsc
Very hopeful
This guy sounds like Moe
from The Simpsoms
12 years ago I was 8
Thank u sir
is this an american David Bowie helping us out?
2020!