Simple Bacteria Genetic Engineering
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- Опубліковано 23 січ 2020
- Genetic modification sounds like something that would be very complicated and difficult to do but the reality is that couldn't be further from the truth. With the right tools and techniques it's something that can be done on your kitchen table if you really wanted. In this video we explore how bacteria can easily be modified to produce a range of fluorescent proteins and even bioluminesce.
More resources:
Building an incubator/heatblock - • From Beer Cooler to a ...
DNA Extraction - • Extracting Spider/Bact...
Running a Gel - • Gel Electrophoresis: H...
Building a Gel Doc - • Building a Gel Doc - T...
DNA transcription and translation - • DNA, Hot Pockets, & Th...
Spider silk project overview - • Spider Beer - Making Y...
Sebastians pages:
Instagram - / atinygreencell
Bionomica labs - binomicalabs.org/
David:
Channel - / @davidishee
Stores to buy reagents:
bio-world.com/
www.phytotechlab.com/
www.goldbio.com/
www.carolina.com/
amino.bio/
www.addgene.org/
neb.com/
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_____________________________________________________ - Наука та технологія
2020: How to make glowing bacteria
2021: How to genetically engineer bacteria to produce jet fuel
It's been done. not overly difficult, but I find it boring so won't.
@@thethoughtemporium I recently read about a kind of "artificial leaf" technology based on cuprous oxide (as a catalyst, it isn't used up in the process) that will take sunlight, carbon dioxide and probably water and turn it into (some precursor of) methanol. Idk if this is your kind if thing, but it'd be amazing to see a video on this!
EctoMorpheus Yeah... graphitic Carbon nitride can do this also and is way more environmentally friendly
March 2021: How to genetically engineer bacteria to produce steel beams.
Sepember 2021:
@@SahasaV When it turns into something else and steel starts growing in random acceptable areas. I wonder if it would be possible for a natural organism to produce things that look unnatural. Like a plant that just thrives in gasoline or oil and breathes in the vapors and in turn produces something that makes itself and the source fire resistant.
"You add resistance to antibiotics and a lot of researchers do this"
If I die by a fluorescence antibiotic-resistant bacteria, at least I know which people to haunt
Dont reveal my future plans
Fluorescent*
at least it's only one type of antibiotic and not all of them lol
@@quappo you're just as bad as the glowing bacteria.
@@JGHFunRun thanks now I can just add that in my list
It’s insane that just a few decades ago this was unthinkably cutting edge technology, but now it’s something college freshmen can do in the first semester of bio lab.
You don't even need to be in college for it
yup, I did it as a junior in a public highschool
@@ivangarcia-lopez2236wait really?
@@devonharvey8414 bay area, so biotech has strong influence in what we do in class. I haven't heard of many other highschoolers doing it, but just know that some highschoolers are already doing it!
And soon the gatekeeping will entirely stop for bio-engineering and we can all do it at home (as this whole project is awesomely about).
So could you use these proteins and incorporate them into spider silk to eventually make genetically engineered silly string
Yes, like home-brewing alcohol then having great fun. So a lot like home-brewing.
Beer was mankind's first foray into biotechnology 😁
@@MandrakeFernflower technically wines and ciders were, but close enough.
Also glowing spider webs
@@theapexsurvivor9538 I thought it was mead?
"It's cyberpunk as hell" is always at least among the top 3 reasons to do anything in biohacking.
until now... because cyberpunk failed to come close to the hype.
@@siiioxide7807 I was thinking about the genre rather than the game when I wrote that lol
biopunk
Also among the top three reasons not to do it.
Someone is going to crispr themselves so they glow, I just know it.
@Heather Petersen i saw someone try on youtube, they failed. they didn't use a virus to deliver it and instead tried to tattoo it onto themselves. it just got digested by white blood cells.
You would have to do that to sex cells because you can't modify 1,000,000,000 cells at once.
@@gamestuff5944 you don't have to. I thought if you modified your dna, your body would absorb the changed cells. But if you change them and stick them into your bone marrow, apparently they change you..may not have to hit bone marrow depending on what you're trying to do
Failed man, it was bad, I was didn't know the liquid that flows in algae was poisonous
@@spec_wasted 😂😂😂
I wanted to pass on a lab tip that someone brought to our lab a few years ago that blew all our minds, and might help you out. Spreading bacteria is super easy with glass beads. I think they're the same kind that jewellers use for grinding media, they're clear, maybe 2-3 mm in diameter. Basically, you pipette your bacteria onto the selective plate, pour a small bit of beads on the plate, put the lid on, then shake the plate side to side. The beads spread the bacteria super uniformly, and you can spread many plates in parallel. Then, you can dump them out into ethanol for cleaning and re-use.
Awesome!
Oh yeah plating with beads is how I grow up transductions or transformations every time. It’s perfect for getting an even spread of colonies!
Toss a coin to your teacher...
how does this not have a single reply
single reply
@@julian-io5wl shutup
@@obamamanmanman8734 shutup
@@horrificminecraftgameplay5884 shutup
Hey man, thank you! I want to let you know that because of your videos, I've decided to get my undergrad degree in biology. I've been putting off using my GI bill, and I've been working trade jobs since I got out of the military, but your videos helped me see how cool and interesting Biology and Microbiology can be. I'm starting in the summer semester, so I'm currently brushing up and studying this semester.
@@miltonferreira9287 its hard to change things up, but honestly give it everything you got. You might feel like and imposter sometimes, but work as hard as you can and you will get through to the other side. Made the decisions to go back to school for computer science and Im never going back :()
Sounds great, best of luck.
Good luck!
Doing my masters in MolBio. Do it, it's so incredibly fascinating :)
RIP bro, it is never never never fun, the books and the way they teach it, not fun
Talking about India, that's where I'm from
Stop me if you've heard this one before:
DIY genetic engineering and buying plasmids, set in a highly capitalistic society.
Would you kindly make more of this great content?
A man chooses, a slave obeys...
Atlas Hugged ur talking about politics
@@SameLif3 They're both referencing Bioshock, a video game.
"the concept was sound"
We must overthrow capitalism.
cant wait for a C to Gene compiler :p
Nah program entire cells in verilog
Minecraft for plasmid when?
@@starletscarlet I don't think bacteria has enough memory for the JVM :p
@@starletscarlet how much rna are you willing to allocate for Java?
1 year late, but "GeneCC"
“What are you doing?”
“Steaming my face”
“To open your pores?”
“Yep”
“To get impurities out?”
“To let the dna IN”
well, programming hello world is easier than growing fluorescent bacteria
Not if you're programming Hello World on a PlayStation 3.
@@ryno4ever433 Well, you're not wrong.
@@kozmaz87 yeah, that's true.
@Heather Petersen try making it by logic gates
It wouldn't be if you had fluorescent bacteria
"EVERYONE'S first experience with genetic engineering"?!....I really am out of the loop
Everyone, who has experienced genetic engineering
Haven't you heard? It's all the rage with the youngsters.
Tumbled Dry
AP biology in American high schools
get with the times nerd. all the cool kids are doing it
He means in classes. AP and collegiate biology classes regularly perform this experiment. I've done it twice as a student.
Bacteria are cool. You can convince them to make virtually any protein, provided you can obtain or produce the gene to do it.
Semi-related: any update on your lactose intolerance cure?
Adding myself to qestion
I wanted to know more about that myself. I'm not lactose intolerant but my girlfriend is. Something like that could give a better quality of life to millions of people. That should not be confined to a youtube video.
I agree, I'd like him to talk more about it
👀
Yes, I'd love to hear some news too.
We did this in our biotech (high school) class!!! I was really surprised to see you doing this, I thought it was a basic experiment. Like a baking soda volcano.
It is. But I needed to show it once so I can reference back to it in future episodes.
I like to pretend I understand the things being talked about in the video
MarsOz who does not
Eventually you will
The His6 tag after the open reading frame on the plasmid encoding the red fluorescent protein is six codons encoding histidine. Histidine will bind to metal ions like Ni^2+ allowing you to separate the recombinant protein by running lysed cells on a nickel column. The recombinant protein will stick to the column and everything else will run through. The recombinant protein can then be eluted by addition of imidazole (histidine has an imidazole group in its side chain) which will compete with the histidine residues for binding to the nickel column and the recombinant protein will be pushed off of the column.
Everyone here does.
Me too a lot of it just sounds like interesting gibberish
Damn we're doing this experiment in biochem lab this quarter. Super cool to see this pop up in my feed
I find it interesting that by watching these videos for the past year I have kind of learned what he is talking about. Without looking anything up or really doing any external research I am learning. I believe that people learn to speak using this same method.
You and applied science are the only channels I have notifications on for.
Edit: 9:39 is a little too real for me.
What other channels do you subscribe to?
Dude, youre missing Tech Ingredients. You can't leave them out of exquisite original content club.
I love abm.
Ok it's basically assembly.
Pretty much. If all the instruction paths are being executed concurrently.
@@benjaminmiller3620 Then, it's like HDL for FPGAs and ASICs?
Lol as a computer science student biological processes are so fascinating. There are so many parallels to the way we engineer computers.
@@woofcaptain8212 Bioinformatics is also critical in helping us understand what the heck is going on and what to do next.
I've been following videos like this for years because I find this whole process and the science behind it fascinating. Sadly, the country I live in has such strict laws regarding genetic modification that it's practically impossible for any private person to do this.
I feel like its an excuse to not try lol I'm sure you can do some small experiment without problem
@@RomanLeBg If the equipment weren't so specific, sure. But "I swear, officer, I just ordered these plasmids and the CRISPR starter kid from overseas so I coud display them on my shelf, I had absolutely no intention of using them" doesn't sound like a very good excuse
@@RomanLeBg Nah. you can get into really big legal trouble here (Germany). Even ordering stuff like Glowfish is prohibited and punished pretty hard. I´ve studied biotechnology and must say, that i´ve relaized that there really is no other legal way to do it, than to do it in a approved lab etc.
Maybe I should stock up on crispr in my country
@@MANUakaSHUFFY Go to another country and bring it to Germany. No borders remember.
I remember doing this in my biotechnology class, back in high school.
Fun Times!
Your videos are so freaking great! So descriptive and informative always, keep up the hard work!
This video is freaking awesome! Extremely interesting and well presented, I learned so much. Thanks for sharing.
The Thought Emporium: Let's make antibiotic resistant bacteria!
Everyone else: WHY?
The Thought Emporium: They will glow cool.
Isn't mutating DNA in labs how they start the zombie apocalypse in all the movies?
While it's technically antibiotic resistant bacteria this ain't the stuff you get from the pharmacist.
@@miltonferreira9287 makes it less scary. Imagine multi colored glowing zombies running after someone. "Pride" zombies.
@@miltonferreira9287 scary but easier to detect at night? I hope its one of those weak slow moving zombies, not those world war Z types.
@@Flying0Dismount zombies are stupidly impractical they would be wiped out in 2 to three months in real life
This video is so informative! This semester we were actually supposed to extract and do various folding experiments with eGFP from e. Coli, but due to COVID our uni is closed for the time. Our professors were kind enough to take videos of the whole process, but it can't really compare to the real thing. Thank you very much for making this video!
Very awesome. Didn't though genetic modification was this straight forward.
I appreciate the use of those 3D printed tube holders :).
P.s. love your videos. No other UA-cam channel like it that posts such a wide variety of amazing science experiments with such a high level of cool sci-fi feel to them :)
Do you know where I can get the file for it ?
Hi! This has been a really, really great demonstration and explanation of gene modification and insertion. It is so good I will probably use many of your descriptions in my classes! Good job!
Bruh, I'm a network engineer/cybersecurity consultant/among other things, and I've always had an interest in becoming a biohacker just never really had the time & money for it. After watching your video which has definitely calmed my anxiety of feeling overwhelmed from how complicated the process just -has to be- I realized that with my IT background this will actually be an awesome and dare I say easy hobby for me to pickup (referencing knowing how to splice a UDP/TCP packet, and its similarities to gene engineering).
Definitely hit that liked button and subscribed, looking forward to watching all of your videos, and will for sure try to send some coffee funds when I could afford it (let's just say I have a list, but you've definitely been added and easily in the top 3 influencers I'd like to support when I eventually could).
Dude. Rad as hell. I agree the flower concept is amazing and the colors he got so far are incredible. I'm no chemist or biologist, but I am an artist, and I am in love with the vibrancy yall are able to produce.
Today I learnt people just ship eachother DNA like its nothing. Just wild
We've been shipping DNA to eachother since the dawn of time. ;)
@@unclekanethetiberiummain1994not you though 😢
I shipped DNA to your mom the other day
"Less is more with DNA"
That's what my grandpa used to say
Haha inbreeding
I feel like I learned so much watching this! Thanks!
Love that this information is free on the internet, thank you!!!
Litterly just did this in my bio class, great video!
I am looking forward to Styropyro's video, whether it succeeds or not!
I think it's been out for a while.
@@crackedemerald4930 Odd, I can't find it!
I did this in high school and it was easily my favorite lab
This bring back memories of my graduating high school project which examined the bactericidal effect of capsaicin on a variety of skin bacteria (plenty of control flaws but I couldn’t afford a single strain of bacteria or pure capsaicin).
In my study I did *plenty* of agar plates.
I did this when i was 14-15 i was the only one to get it right in my advanced bio class.
hello, sorry for being really lateeee, how did you obtain the bacteria? did you experiment with e.coli too? can i use the salmonella bacteria in raw chickens in this experiment? been wanting to test this out for myself. thanks!
I like the programming analogy when tinkering DNA is involved, it just when people begin to say that DNA is a manufactured code which is not good.
Also with programming it was built by humans and is about 200 years old and biology is literally a language built by the universe and stress tested for millions of years.
No analogy is perfect. That seems the be the bit that trips people up. And while this one seems to be pretty good, it actually starts to break down really quick once you start digging into the details. The "runtime" for DNA is way more complex and unpredictable than any digital computer.
@@gh0stmast3r Which considering there is no design in it makes it actually quite shitty and fragile. It's stress tested for numbers game, not individual reliability. Just look at promoters not having any particular common pattern.
fluffy_tail programming can be though, even though they said it’s not(revolutionary)
aaah!! so excited to do this at university! this career is truly wonderful!! subbing ♥
Thank you for the web sites assessment sharing. You are realy generous to share your experience and advices...
Can someone make some fluorescent yogurt bacteria? That would be cool.
Yogurt generally isn't a monoculture but I love the idea of that.
Its already been done
Loved this but as a microbiologist I do need to point out a few errors in your antiseptic methods. You shouldnt open eppendorf tubes with the same hand as touching the lips could contaminate the containments. 2; you should have a flame nearby to direct airflow away from plates when you're pouring media. 3; you should use a hokey stick type spreader and a spinning plate for spreading rather than Q tips :). You'd get a much better spread. There are other things like always having correct airflow (flow bench/ work near flame). Also for storing your bacteria you might be better off if you plated your bacteria and inoculated a couple of plates from which you could collect bact via loops later should u need to. I loved the video tho, keep it up :)
Oh I almost forgot; leaving the media/broth container cap on the ground can deffo contaminate stuff u should also watch out for that :)
There was a flame going off camera in many of these shots. Also they weren't q-tips, they're individually packaged sterile swabs. I hate hockey stick spreaders honestly, the swabs work like a charm. The entire lab is a positive pressure room fed by a hepa filter, and there's a second hepa filter next to me. Also there's a massive uvc bulb above the workspace that I run occasionally to nuke the whole room.
The contam on that plate was likely because I waited too long and let the media cool too much before adding the charcoal. Had gotten busy doing other stuff waiting for the autoclave to cool. Contam on the rainbow is because of how fricken long it took to make that so the open time was high.
I store plated bacteria as well, but I prefer to store bacteria as stabs. That said, I keep stabs, plates and pure DNA of every plasmid I have.
I was taught in school to leave lids face up on the ground if I can't hold it with my pinky, and have almost never had issues.
I am looking to add a filtered air blower box/laminar flow box onto my work table to help lower contam rates even further for doing more delicate work like plant tissue culture.
Thanks for the tips though and I'm glad you liked the video :)
@@thethoughtemporium sounds like you know what you're doing! Good look in the future and keep the good content coming ☺️👍👍
Thank you. I have been fascinated with bioluminescence and fluorescing proteins.
This is a really amazing video. Thanks so much!
8:25
I got visions of high PhD students in the StonyLab.
"Dude, these agar plates are giving me the munchies".
This video was so helpful for noobs like me interested in biology and genetic engineering. Thanks for putting this together! Keep up the great work! :D
As usual with your channel, Great video! Thank you for sharing your knowledge on this very interesting subject. I previously worked with quantum dots and the spectrum of vibrantly fluorescing test tubes brings back pleasant memories :-)
17:50 that's a really fitting Express in considering the last video you posted
I know nothing about microbiology but I want to learn enough that I can do a project like this at home. Should I take a entry level bio lab at school or can I learn all of this on the internet?
Yes take it, and on the internet you can do everything you want to learn
Bacteria: Exist
Humans: Art
where was this video when i was in 3rd semester? thank you and great content as always!
I did this too in my biotech undergrad. was super cool. We used a lot of weird fluorescent proteins for our iGEM project, even one with an ubiquitin residue which has a half life time of only 20min so you can see with your own eyes how the production of it stops.
My teacher is searching for german videos like this😅
I watch your videos since 2018 and really like them but many germans don't understand english and please keep on making these Videos🙃
Shame on every german who doesn't understand english.
It really freaks me out when I want to show my friends such an awesome video and they just don't understand it...
Dann sag den Piefke sie sollen über den Tellerrand schauen und Englisch lernen.
Learning English as a German is easier than learning genetical engineering since the languages are very similar.
Man, you're really making me wish I took bio in highschool
Wow! Plasmids, bacteria, etc! Would you kindly make more videos on this?
I liked the DIY centrifuge! Cant go as fast as the mini you showed yet DIY science is the best kind
Why are some segments of the plasmids read backwards and some forward/on one strand Vs the other?
Edit:seemingly, going by arrows
DNA is read in one direction-- from the phosphate end to the hydroxyl end (5' to 3'). The two strands that make up DNA run in opposite directions (anti-parallel). If you've got a double stranded DNA helix going "up and down", the "left" one is able to be read "up" and the other read "down".
Since DNA is read in groups of 3, there's 3 ways to read a single strand. as an example, if you have "ATCATCATC" you can read it "ATC...ATC...ATC" or "A...TCA...TCA...TC" or "AT...CAT...CAT...C".
You also have a reverse strand "TAGTAGTAG", which can be read 3 different ways. So there are 6 unique ways to read DNA. For further information, look into "reading frames"
Already waiting for pyro's video!
Your Channel is one of those wildly insane ones on UA-cam - your experiments cross so many "normal" boundries and I absolutley adore it.
It's a great time to be alive where you can just sit in front of your PC and watch someone play around with bacteria, lmao
I dont know the first thing about genetic engineering or biology but I love watching these. They're so interesting
OOOHHH that is so cool. There's yeast that can make beta carotene??? Can we use them in bread??
silk beer coming soon
me: YEEEEEESSSS
These videos always intrigue me, since I'm trying to get a degree in microbiology.
An interesting video. Thank you for sharing it.
Grow little bacteria at home, pick your color and pit them against each other in a race to cover the most space!
I would very much like glow in the dark oak trees... I would pay
Thanks for the supplier list. I wish I had that a year ago. I bought most of my supplies on eBay but I would need to find a different seller for almost every reagent.
I remember doing this in my genetics lab! Fun stuff!
I remember doing this in biology to make bacteria glow in the dark
back in my day, we cut apart frogs in biology and Margaret Mary fainted XD
Soon my guys, soon we will get cat girls.
"daddy why do we look this way?"
"Well honey that's because our forefathers were idiots and didn't make a backup."
"Terrifying!"
"Indeed."
Nice video! :)
I believe that the non-transformed colonies you mention at 17:58 are perhaps not due to rapid mutations, but rather the secretion of beta-lactamase, the ampicillin resistance protein, from transformed bacteria. The antibiotics around transformed colonies will be degraded, allowing growth of non-transformed bacteria (satellite colonies). It’s a common problem using only ampicillin, although I usually see them slightly smaller after overnight incubation than on your plate. Just to be cautious, pick from the middle of your biggest colonies if you can’t select with UV or another antibiotic like carbenicillin. It will increase your odds for succes :)
I know it’s not legal but hypothetically could you make a human glow? Like A really cursed baby build a bear
how would you get human cells?
dead children?
@@MicahPachirisuGuymy taking samples from any living human being
Aren’t DNA sequences just LIFE CODE
And code language is chemistry.
Dude u say it’s so simple I am going to have to watch it this repeatedly
YESSSS BEEN WAITING FOR THIS
make glowing human babies
changing my dna so I'm not related to my dad, thanks
*OOF*
So excited for this video! :D
Big fan of Sebastian and Binomica.😊
Better wash your hands fast, here come the TURBO E.COLI
Cool mod
Steam link please
Nice work BioHacker!
awesome to see styropyro collab, amazing
4:45 SexAI
UA-cam: *demonetized*
Buying colored highlighter markers for a few bucks seems cheaper and faster.
I love how you explain it and not just do it
Man I love your centrifuge design! 🤣👌
Idea: gut bacteria to produce insulin.
some people are pro life...
some people are pro choice
bruh im prokaryotic
gold
"Eukaryotes!"
"What do you mean im a karyote?"
This video is soo good at this time, while i'm missing out in my microbial genetics lab because of COVID
Love the continued dunking on The Odin. As a part of an attempt to do CRISPR cheaply for molecular cell bio classes at my community college, my research lab partner and discovered that their stuff is utter garbage. The sent us a ton of contaminated stuff and then took tons of heckling by our professor to get replacements from.
Amazing content, thanks
Sick video man ! Love your contend.
I really want to get into this stuff. I’m in a tiny dorm room at the moment so I have to wait until I get my own place to setup something more proper. One of my friends is going into biochem and another into biomed engineering so I could probably get help from them at some point. I also looked on dybio.org and there is a local lab in my city so I’ll have to check that out at some point. I’m in Canada but I have a PO box in the US and I wonder if companies would consider that a commercial address if I set up a sole proprietorship.
Whatever happens, I’m really excited about this stuff. I definitely want to try out the purple chromoprotein at some point. It would be cool to extract it or even put it in something different but that’s a bit far off my knowledge and skill at the moment.
i will watch every ad for this channel 👏🏻
When put in the ice bath the CaCl2 helps binding of the plasmid DNA to the bacterial LPS and then upon a brief period of heat shock the facilitation of uptake of Plasmid DNA takes place!