Thank you for such clear explanation of such complex process. 95% of the genes are alternatively spliced -- that is a shocking and very useful information.
Hi professor! I found your video helpful but I do have a question. If alternative splicing gives different sequences how can that be a good thing? I understand that it does bring up diversity but isn't the whole point of making the RNA in the first place to have the code to make specific proteins? How can alternative splicing be helpful when it codes for different proteins? Isn't this close to a mutation? I am just confused about this being a good thing. Thank you for your time!
Hi there! It can be a good thing because it can result in more than one functional protein. Via alternative splicing, it is possible to make different proteins that are similar enough that they carry out related functions that are just a little different from each other. For example, the Drosophila Down syndrome cell adhesion molecule (Dscam) gene has 95 exons. Drosophila can make 38,016 different isoforms by the alternative splicing of these exons. These different isoforms will all have slightly different activity in the cell. That is a lot of variety! You can see some more examples here: en.wikipedia.org/wiki/Alternative_splicing#Examples
Yes, multiple RNA transcripts can be made from a gene and exist simultaneously in the cell, with some being spliced one way and others being spliced a different way.
Alternative splicing allows eukaryotic cells (including human cells) to use the same GENE to get more than one PROTEIN, just based on which exons are kept in the mature mRNA transcript and why are spliced out. In other words, there can be multiple similar/related proteins possible from one gene.
Yes! It doesn’t alter genes directly, but Alter’d their expression, which creates differences that can be acted on by natural selection. More here: news.mit.edu/2012/rna-splicing-species-difference-1220
Please invest in a wearable microphone. Otherwise really good video! Also good with the short selected theory on the left side of the board. Thanks again!
I got it the number from here: pubmed.ncbi.nlm.nih.gov/18978789-deep-surveying-of-alternative-splicing-complexity-in-the-human-transcriptome-by-high-throughput-sequencing/ It is from 2008, so could be out of date; but I think it is still definitely higher than 59%
U teaches really well..i am become a huge fan of u..can i get ur email or number mam??..actually i want to shootout some problem related to genomics..thats why i want this.if u dont mind so could u??
That was a very clear, helpful and useful lecture. Thanks a lot from Turkey!
You are very welcome!
Very good and simple explanation.
Greetings from Germany
Yeah
Best faculty I ever found to make things easy. Thanks!
Beautifully explained and illustrated keep up that great work! Thank you for your time!
I also was shocked when I first read about alternative splicing. This is an amazing mechanism
Nice video! It is the first one I've found that explains the regulatory mechanism, and not only what it is alternative splicing. Thank you :)
Yea
that was so helpful thank you from Canada!
Thank u miss, Ur all videos are really simple explained and helps a lot in my studies☺️❤️💕
Lecture was amazing, thank you for making it simple to understand.
I LOVE THIS WOMAN! THANK YOU! life saver!
Thank you very much mam
Very good explanation, Greetings from Kashmir
Thank you very much!
This is very much appreciated, thank you. Adel from Egypt.
It was a great video and very good information. Thank you.
Thank you for such clear explanation of such complex process. 95% of the genes are alternatively spliced -- that is a shocking and very useful information.
Excellent..
Thank you for such a clear explanation, helped me a lot. ❤
Very helpful indeed. Thanks from Brazil!
Awesome. Thank you very much!
Thanks for the class. Great.
Super helpful and clear, thanks!
concise n helpful, thanks a bunch
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Thank you. Very helpful.
Your explanation was fabulous ♥️❤️ Thank you very much 🙏🏻👍🏻🌹
You're welcome 😊
Very helpful! Greetings from Germany
This was so simple and informative!
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Nice explanation. Greetings from Japan.
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very good explaind ! Thanks
Very good explanation, thank you
Greetings from Perú
My best friend’s husband is from Peru. Greetings back to you. :)
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Lecture is well made. Thanks a lot!!
Very good!
Very helpful, thank you!
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Thank you professor :)
You are very welcome 🤗
Thank you very much great explaination make the topic very interesting thank you
thank you very much that was a great video!
Please also make a video lecture on the generation non-coding RNAs from TE. Thanks!
thanks!
Brilliant
Bravoo!!
excellent video!
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Awesome
Hi professor! I found your video helpful but I do have a question. If alternative splicing gives different sequences how can that be a good thing? I understand that it does bring up diversity but isn't the whole point of making the RNA in the first place to have the code to make specific proteins? How can alternative splicing be helpful when it codes for different proteins? Isn't this close to a mutation? I am just confused about this being a good thing. Thank you for your time!
Hi there! It can be a good thing because it can result in more than one functional protein. Via alternative splicing, it is possible to make different proteins that are similar enough that they carry out related functions that are just a little different from each other. For example, the Drosophila Down syndrome cell adhesion molecule (Dscam) gene has 95 exons. Drosophila can make 38,016 different isoforms by the alternative splicing of these exons. These different isoforms will all have slightly different activity in the cell. That is a lot of variety! You can see some more examples here: en.wikipedia.org/wiki/Alternative_splicing#Examples
Thank you!
What about alfa-tropomiosyne? Is there any difference in the splicing process between exons 2 and 3 and 7 and 8?
Thank you thank you you saved me
Very helpful thnkuu😍
عظيمة والعظمة لله
That was helpful, thanks!
Thank you so muchhhhhhhhh
Is it possible to have 2 or more transcripts for different proteins simultaneously from a single gene?
Yes, multiple RNA transcripts can be made from a gene and exist simultaneously in the cell, with some being spliced one way and others being spliced a different way.
Good videos
Thanks! 😀
Love From India 🇮🇳
Thank you so much 💓 too helpful
Thank you very much! You look like emilia Clarke by the way
very helpful！
I just want to learn about splice donor/acceptor sites and where they can be found!
What's the importance of Alternative Splicing?
Alternative splicing allows eukaryotic cells (including human cells) to use the same GENE to get more than one PROTEIN, just based on which exons are kept in the mature mRNA transcript and why are spliced out. In other words, there can be multiple similar/related proteins possible from one gene.
Thank you! 😄
Does alternative splicing create genetic variation and evolution?
Yes! It doesn’t alter genes directly, but Alter’d their expression, which creates differences that can be acted on by natural selection. More here: news.mit.edu/2012/rna-splicing-species-difference-1220
What can be removed from pre-mRNA during alternative splicing?
introns are spliced out, and sometimes part or all of certain exons that would be left in the mRNA in alternative forms.
please made assignment on Differential RNA Processing
Differential RNA Processing is another name for Alternative Splicing.
you'er the best
Thank you, great lecture!
Please invest in a wearable microphone. Otherwise really good video! Also good with the short selected theory on the left side of the board. Thanks again!
Just FYI; you cannot skip the 1st or the last exon
Sorry but can you tell me the reason
Thank you for explaining AS generation mechanism in a good and simple way. I am also looking for the AS analysis pipeline using the Linux platform.
I’m sorry, I don’t know much about Linux. I used Unix for a semester in college, but I’ve forgotten it all. :(
Alternative splicing=gene expression regulation?
Yes, it is one form of regulation of gene expression
are u sure that it is 95% of multi-exonic genes being alternatively spliced? I learned 59%
I got it the number from here: pubmed.ncbi.nlm.nih.gov/18978789-deep-surveying-of-alternative-splicing-complexity-in-the-human-transcriptome-by-high-throughput-sequencing/ It is from 2008, so could be out of date; but I think it is still definitely higher than 59%
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A-MAZ-ING
Screw the lecture. Are you single
You are looking alike to my gf ,Indian . 😘
U teaches really well..i am become a huge fan of u..can i get ur email or number mam??..actually i want to shootout some problem related to genomics..thats why i want this.if u dont mind so could u??
You look like emma watson
M'dm u r vry beautiful along with good lecture
R u single?
Do you have a boyfriend?
No, I have a husband.
Biology Professor for how long 🧐?
Quite awhile. We’ve been together over 10 years
@@BiologyProfessor but I want you 🥺