I would measure the cell count of your culture flask and then determine how much cells you want to have per volume... You can use a 10-fold serial dilution here, might help!
I have a question. The stock volume does not determine the dilution factor right? Like if I mix 20g of solute in 200ml of sterile water, can I take just 1ml for the dilution? Because AI is telling me I have to take 40ml and it just doesn't make sense.
Hi there I have a question let say I have very high absorbance reading so i dilute for 1:10 and then 1:100 how to calculate the absorbance? Let say at 1:10 the reading absorbance is 2.5440 so what is the exact absorbance?
absorbance might not translate directly from the original solution to the diluted one. but you can use the diluted absorbance to find e,g, a concentration, and the concentration of the original is explicitly 10x that.
Thank you so much i have been struggling recently in the labs and needed more explaination. This really helped
Thank you so much, it was so easy to understand
Good simple explanation. Thanks!
Simple and Short
question: why is the ratio given as 1:10 and not 1:9, since it is 1 part solution with 9 parts water?
Because the total volume is 10. And the main part is 1 part of the total 10.😊
thanks. Illustration very good
Wow this was great. Thank you.
I still don't quite understand. In my case I have colloidal silver 500 ppm and I need to make it into 50 ppm??? How???
To me that seems to be a classical 1:10 dilution
Were you able to get your silver ppm down after all?
Jazak Allah😊
Question, how would you apply this to decreasing the number of cells for cell culture just like you mentioned here?
I would measure the cell count of your culture flask and then determine how much cells you want to have per volume...
You can use a 10-fold serial dilution here, might help!
I have a question. The stock volume does not determine the dilution factor right? Like if I mix 20g of solute in 200ml of sterile water, can I take just 1ml for the dilution?
Because AI is telling me I have to take 40ml and it just doesn't make sense.
Question, If I need each tubes to have 10 mL in total volume at the end of the process how do I do that?
If you really need 10ml of each: you can also do something like 1.1ml + 9.9ml (11ml in total)... than transfer 1ml into the next tube and so on...
I have to make 100 ml of a 10 fold dilution of vinegar. How do I do that?
thanks for the simple explanation 😄
Hi there I have a question let say I have very high absorbance reading so i dilute for 1:10 and then 1:100 how to calculate the absorbance? Let say at 1:10 the reading absorbance is 2.5440 so what is the exact absorbance?
absorbance might not translate directly from the original solution to the diluted one. but you can use the diluted absorbance to find e,g, a concentration, and the concentration of the original is explicitly 10x that.
Thank you very much it was an great information
It was quite east sir😢..thqnuh so much 😊
Hey sir, the final volume of the ten fold expirement is 9 mL?
Yes, because from the last test tube we discard that 1ml
Thank you!
Helpful!
Thank you 😊
Thank you
Good explain
Thanks 😊
nice video 😍
It can't be 1:10 as there would be 11 parts and you have 10 parts.
that is what i thought too
Helpful
Thank sir
Nice
cool
Interesting, ich glaube du bist deutscher (:
Vielen grüßen von Deutschland
I don't understand this
Thank you so much, it was so easy to understand
thank you