How to prepare a Serial Dilution

[@charlottewright929]

Thank you so much i have been struggling recently in the labs and needed more explaination. This really helped

[@luckyduke7159]

Thank you so much, it was so easy to understand

[@sethdjanboakye]

Simple and Short

[@Abialmedicos]

Jazak Allah😊

[@marcoromo9252]

Good simple explanation. Thanks!

[@reamageorge9648]

thanks. Illustration very good

[@osmirod1]

Wow this was great. Thank you.

[@Imkevinplayz]

Thank you

[@osmirod1]

Question, how would you apply this to decreasing the number of cells for cell culture just like you mentioned here?

[@henrikslab]

I would measure the cell count of your culture flask and then determine how much cells you want to have per volume...
You can use a 10-fold serial dilution here, might help!

[@sorlos]

Helpful!

[@Palayedeuhm]

Thank you!

[@انتصارالشرعبي-م4ط]

thanks for the simple explanation 😄

[@oldlady8988]

I still don't quite understand. In my case I have colloidal silver 500 ppm and I need to make it into 50 ppm??? How???

[@henrikslab]

To me that seems to be a classical 1:10 dilution

[@randall777]

Were you able to get your silver ppm down after all?

[@bahiachibi895]

Helpful

[@halafr5861]

Thanks 😊

[@misterJoStar-g3f]

Question, If I need each tubes to have 10 mL in total volume at the end of the process how do I do that?

[@henr1k036]

If you really need 10ml of each: you can also do something like 1.1ml + 9.9ml (11ml in total)... than transfer 1ml into the next tube and so on...

[@Abialmedicos]

It was quite east sir😢..thqnuh so much 😊

[@jeniferhernandez3465]

Thank you 😊

[@ivyscreativediary4836]

I have a question. The stock volume does not determine the dilution factor right? Like if I mix 20g of solute in 200ml of sterile water, can I take just 1ml for the dilution?
Because AI is telling me I have to take 40ml and it just doesn't make sense.

[@TriniCanCook]

I have to make 100 ml of a 10 fold dilution of vinegar. How do I do that?

[@prince_vegeta124]

Thank you very much it was an great information

[@rihamalwan9229]

Good explain

[@h.axb_]

nice video 😍

[@padhmapadhma9273]

Thank sir

[@Jibreel-u2v]

question: why is the ratio given as 1:10 and not 1:9, since it is 1 part solution with 9 parts water?

[@aishwaryaks224]

Because the total volume is 10. And the main part is 1 part of the total 10.😊

[@gilbertkapapa5282]

Nice

[@abitofambience]

Hey sir, the final volume of the ten fold expirement is 9 mL?

[@Quolon]

Yes, because from the last test tube we discard that 1ml

[@mazrinamazlan1838]

Hi there I have a question let say I have very high absorbance reading so i dilute for 1:10 and then 1:100 how to calculate the absorbance? Let say at 1:10 the reading absorbance is 2.5440 so what is the exact absorbance?

[@Nowolf]

absorbance might not translate directly from the original solution to the diluted one. but you can use the diluted absorbance to find e,g, a concentration, and the concentration of the original is explicitly 10x that.

[@daliakhater2558]

cool

[@harry011984]

It can't be 1:10 as there would be 11 parts and you have 10 parts.

[@Pauliwog13]

that is what i thought too

[@timate9370]

Interesting, ich glaube du bist deutscher (:

[@TaibaTheNRP]

Vielen grüßen von Deutschland

[@snumarin]

[@definitlynotbenlente7671]

thank you

[@kameleonfn]

Thank you so much, it was so easy to understand