How to Perform Serial Dilutions in Microbiology

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  • Опубліковано 22 гру 2024

КОМЕНТАРІ • 110

  • @Truck--kun
    @Truck--kun 6 років тому +28

    nice to see someone actually using gloves in demonstration. Many videos don't and that is a safety hazard, glad to see you are doing it right and safely

    • @leonardovasquez9011
      @leonardovasquez9011 5 років тому +9

      Gloves are dangerious if you use a burner for sterile conditions.

  • @hampdengal
    @hampdengal 4 роки тому +16

    This video was very well done. Thank you for the thorough explanation of everything!

  • @bobbymccarrick5114
    @bobbymccarrick5114 4 роки тому +17

    I really enjoyed watching this video. Extremely helpful and well taught.

  • @dapadapjameswalter888
    @dapadapjameswalter888 3 роки тому +10

    thanks for this video... even though we can't perform this in our lab due to covid but this video was so helpful for students like me(microbiology major)........

  • @namulindatabitha1577
    @namulindatabitha1577 2 роки тому +1

    Thank you for the smooth explanation

  • @mdshafiulislamrion4069
    @mdshafiulislamrion4069 2 роки тому +3

    I think the dilutions were plated wrong. The higher dilutions should have less colony. But in the 6:01 time of the video, the higher dilution had too numerous colony to count. And the lower dilutions had countable colonies. The way she kept those plates in front of her made this thing wrong. She took the highest dilution numbered plate but put the 1 mL liquid from the lowest dilution tube.

  • @aaronayubasamaila8821
    @aaronayubasamaila8821 3 роки тому +3

    Nicely done. After incubation you said some plates gave you too numerous to count number of colonies while the last plate gave you countables. When recording the results which should we record?

    • @fizakyeomz-6744
      @fizakyeomz-6744 3 роки тому

      plates that have the number of colonies within 30-300 based on what I've learned... more than 300 is too numerous too count while less than 30 is too few to count

  • @avalle93
    @avalle93 5 років тому +4

    why did you put the first dilution solution into the 10^6 plate? shouldn't be on the 10^1 plate? did anyone noticed that?

  • @jkgou1
    @jkgou1 4 роки тому +3

    Would you please tell us what the optimistic vortex mixer rotation speed is in rpm (round per minute)

  • @joyoluchi9012
    @joyoluchi9012 2 роки тому

    Nice!!
    But please can I see a sample of a practical report done on Serial dilution?

  • @jiorama5459
    @jiorama5459 8 років тому +12

    i have a question, why do we do serial dilution? why can't we add a small sample to a large volume of the dilution solution ?

    • @Microbiologics
      @Microbiologics  8 років тому +9

      You can do that. It is just important to make sure that you are measuring your small sample volume accurately. As the volume of a measurement decreases (1 µl vs. 1 ml), accuracy of the measurement also decreases. So if you need to dilute from 10^7 to 10^2, you either need to do serial dilutions, or you need to work with a large enough volume to ensure that the dilution is as accurate as possible.

    • @sandisonkosi9835
      @sandisonkosi9835 7 років тому +2

      hi, on my view the reason we do serial dilution its because we need to know the amount of bacteria present in the original sample by decreasing their volume

    • @pankajsingh5382
      @pankajsingh5382 7 років тому +1

      Its a standrad process for dilution gives accurate measurment.Here we add a small sample to a measured volume of the dilution solution. We can't think like mixing a smal drop in an unknown quantity of the dilution solution.

  • @mohsinkhan3338
    @mohsinkhan3338 7 років тому +4

    This was very helpful, thank you

  • @mrinalinimore6953
    @mrinalinimore6953 23 дні тому

    When do we have to do serial dilution?
    For what kind of experiments?

    • @nyxsylph
      @nyxsylph 20 днів тому

      Food agencies do this to count the number of bacterias in the food to see if it safe for consumption

  • @ShopperPlug
    @ShopperPlug 3 роки тому +1

    Nice explanation. How can I isolate 4 different microbes from a know sample solution using this method?

  • @raazaaaa
    @raazaaaa 2 роки тому

    is it not necessary to do it inside laminar flow

  • @shahidakhan7896
    @shahidakhan7896 4 роки тому

    Recently did serial dilutions but I didn't get isolated colonies.There was a lawn type growth on the edges and middle part of the plate.I used to spread 200 ul on the plate.please please guide me.

  • @yadhurammahato878
    @yadhurammahato878 5 років тому +1

    please describe the process of making initial stock solution of powdered sample. while dissolving powdered test sample into distilled water, does it get diluted? Also, if 1 gm of powdered sample is dissolved into 10 ml of water, what will be the dilution ratio?

    • @Microbiologics
      @Microbiologics  5 років тому +3

      There are many resources online that can help you determine the correct amount of powdered sample to add to create a stock solution. In general, a stock solution is made by weighing out an amount of the solid (powder) and dissolving it in an amount of solvent to get the desired concentration, or Molarity, of the stock solution. Dissolving 1gm of powdered sample into 10ml of water would yield a 1:10 dilution of the powdered sample.

  • @romilarora8457
    @romilarora8457 5 років тому +3

    Thnku sooo much mam ☺
    Mam is their any video on sample collection n isolation of thermophiles? ?

  • @chieduepiphany9049
    @chieduepiphany9049 6 років тому +1

    is there any video on laboratory method of isolation of actinobacteria from waster sample

  • @saz4217
    @saz4217 3 роки тому

    a question please
    Is E test same as dilution test?

  • @anderfabi600
    @anderfabi600 7 років тому +1

    what do you do with the tips of the micropipettes? the reuses?

    • @LinaYamilette
      @LinaYamilette 6 років тому +3

      You cannot reuse them, they're no longer sterile. They must be thrown out properly

  • @darlenedeguzman4999
    @darlenedeguzman4999 3 роки тому

    I have a q. What exactly did you put first in that tube with phosphate buffer? that solid one.

    • @DrTolis
      @DrTolis 3 роки тому

      That is the bacteria in dried up form. In this form they can be kept for a long time and they came alive when placed in the buffer

  • @humajawed6409
    @humajawed6409 6 років тому

    after transferring and spreading the diluted inoculumn on the agar plate, agar plate should be inverted while incubated ? as the the chance of sample drop off into the lid of the petriplate. kindly guide

    • @Microbiologics
      @Microbiologics  6 років тому

      After spreading the inoculum on the surface of the agar plate, make sure it has been absorbed into the agar before inverting the agar plates during incubation. The length of time this will take depends on the volume used to inoculate the plate and the depth of the agar. Visually check the agar prior to inverting the plate. If the inoculum has not absorbed into the agar, it can drip onto the lid of the Petridish while inverted.

    • @elenaautumnalmermaid6913
      @elenaautumnalmermaid6913 3 роки тому

      Inverting the plate prevents condensation kn the lid and dripping water on the colonies

    • @kizito8977
      @kizito8977 2 роки тому

      @@Microbiologics ❤

  • @mbufonghelen4649
    @mbufonghelen4649 2 роки тому

    how come the highest dilution has the greatest amount of growth? is it not supposed to be the reverse?

  • @storyquill1208
    @storyquill1208 5 років тому

    How do you know how far you need to dilute to get a reasonable amount to count, and how do you know much is growing in the original sample based on the diluted findings?

    • @Microbiologics
      @Microbiologics  5 років тому +4

      Sarah - To know how much to dilute your sample in order to get a reasonable number of colonies to count, you must know what your CFU concentration is to begin with and what you want to end up with. If you are using Microbiologics products, it is easy to know what your original CFU concentration is because we tell you. For example, Microbiologics Epower™ E3 product is equal to 1,000 to 9,9999 CFU per pellet. If you are not using a quantitative product, you will need to make a suspension of the microorganisms, measure the concentration using a spectrophotometer or turbidimeter, and then dilute the suspension until you get within countable range.
      To calculate the CFU concentration of your original sample, you will need to work backwards. Count the number of colonies on your plate and then multiply this number times the dilution factor.
      Check out the Microbiologics Dilutions Guide to learn more: www.microbiologics.com/Microbiologics-Dilutions-Guide

    • @storyquill1208
      @storyquill1208 5 років тому +1

      @@Microbiologics thank you very much!

  • @Dabby3
    @Dabby3 4 роки тому +1

    Nice Video. Though I think, 1 mL on one petri dish is very much and would cause condense water in the process of incubation.

  • @OkohPee
    @OkohPee 2 роки тому

    Excellent job there

  • @huleshsahu7251
    @huleshsahu7251 5 років тому

    Hello.... If found 10 colony from stock solutions than how to calculate cfu/ml... Require any calculations factor?...

    • @Microbiologics
      @Microbiologics  5 років тому

      Calculating the concentration of your stock solution will depend on how you manipulated the stock solution to get a readable plate. For example, if you removed 1ml from your stock solution, plated it, and counted 10 CFU on this plate, your concentration would be 10 CFU/ml. If you removed 1ml of stock solution, diluted 1:10, plated 1ml, and counted 10 CFU, then you would need to factor in the dilution for your calculation. If you have specific questions based on your protocol, please feel free to reach out to our Technical Support Team at techsupport@microbioloigcs.com.

    • @troyjesse7833
      @troyjesse7833 4 роки тому

      Technically, 10 colonies is too low to use for CFU/mL. The standard is to use a plate with 30-300 colonies.

  • @mritunjaykumaryadav2215
    @mritunjaykumaryadav2215 4 роки тому

    What is the amount of innoculum taken?

  • @Pragyan-u2p
    @Pragyan-u2p Рік тому

    Can anyone tell me the name of the vibrating machine?

  • @ray1030
    @ray1030 3 роки тому

    Why don't you work in front of a flowhood? Is the lab room that sterile?

  • @nerghezgull7942
    @nerghezgull7942 6 років тому +1

    Thank you
    Just i have a qution,,,
    What is the liquid you are used to diluttion in a tube.? Is a culturec media or distle water?

  • @jinsuyankeyanke4890
    @jinsuyankeyanke4890 2 роки тому

    How to do with L- rod

  • @whatthehellogolab
    @whatthehellogolab Рік тому +1

    i have an examination tomorrow from all the serological reactions and it seems terrifying 😀

  • @بياناحمد-ق4ه
    @بياناحمد-ق4ه 3 роки тому

    مشكووور كثييير حلووو الشرح

  • @EvelcyclopS
    @EvelcyclopS 4 роки тому

    I was always taught not to keep caps inverted but to asepotcally remove and keep in the hand holding the tube

    • @Microbiologics
      @Microbiologics  4 роки тому +5

      Thank you for your comment! Aseptically removing the cap and holding in your hand is one technique to minimize contamination during a serial dilution alongside inversion on the bench. If inverting onto a bench, make sure this is sterile or perform the dilution series in a biosafety cabinet or laminar flow hood.

  • @amedine2135
    @amedine2135 4 місяці тому

    Procedure for antibacterial and antifungal effect of leaf extract from persea Americana mill

  • @badmashthebadassbadmash3383
    @badmashthebadassbadmash3383 4 роки тому

    Is this method used for sterilization. ..?

  • @littletree5871
    @littletree5871 4 роки тому

    What temperature was used to incubate these plates?

  • @whiskeyzc
    @whiskeyzc 6 років тому +1

    I have a question. Instead of diluting 1ml to 10ml, can we do it from 0.1ml to 1 ml?

    • @victormutindamunyasya692
      @victormutindamunyasya692 6 років тому +2

      yeah you can..but...the reason you dilute 1ml into 9ml to get a total of 10ml is for simple mathematics calculation...your case would be an mathematic nuisance.

  • @victormutindamunyasya692
    @victormutindamunyasya692 6 років тому

    do i need to do this under a laminar flow? and can i only plate the last dilution instead of the whole series?

    • @Microbiologics
      @Microbiologics  6 років тому +2

      This is up to the SOP guidelines you follow. At our facility, we do not use a hood when performing dilutions.

  • @peepdi
    @peepdi 4 роки тому

    Why is soil used?

  • @hongdinh2410
    @hongdinh2410 6 років тому

    How do you spread 1 mL dilution of homogenate onto each plate? We use pipet 0,1 mL or 0,3 mL.

    • @Microbiologics
      @Microbiologics  6 років тому

      If using a 1.0 mL inoculum size, we suggest dividing the inoculum onto several agar plates.

  • @mr.buttercutter9294
    @mr.buttercutter9294 5 років тому +1

    Fantastic explanation mam.thanks.

  • @rashmipandey4408
    @rashmipandey4408 2 роки тому

    Thank you ma'am ❤️

  • @toiladuy1998
    @toiladuy1998 6 років тому

    i have a question. If this method is very accuracy, so when should we perform single dilution?

    • @Microbiologics
      @Microbiologics  6 років тому

      Each dilution is plated on the video as a teaching tool so the viewers will understand what the recovery will be for each dilution. If you do not feel that plating each dilution is necessary, you can adjust your procedure accordingly.

  • @aftabkhan4995
    @aftabkhan4995 7 років тому +1

    thank you
    and what bacteria its is?

    • @Microbiologics
      @Microbiologics  7 років тому +4

      We used E. coli for this demonstration: www.microbiologics.com/0483E7

    • @aftabkhan4995
      @aftabkhan4995 7 років тому

      okay ,thank you
      can you show me how isolate xanthomonas vesicatoria from tomato plant properly?

    • @aftabkhan4995
      @aftabkhan4995 7 років тому

      and what does it mean by 5x10 to pwr 6?

    • @Microbiologics
      @Microbiologics  7 років тому

      5x10 to the pwr 6 is 5x10^6 or 5,000,000 colony forming units (CFU).

    • @aftabkhan4995
      @aftabkhan4995 7 років тому

      If I get 35 colonies on serial dilution of 10^-6 then how I ll write it??

  • @okpeuchenna3656
    @okpeuchenna3656 7 років тому

    thank you. If i pour plates in duplicates, that is, from the 5th and 6th tubes of the dilution, and i take the average of these two, at what power would i report my result, x10^6 0r x10^5?

    • @Microbiologics
      @Microbiologics  7 років тому

      It is not advisable to determine an average from plates prepared from 2 different dilutions unless both are in your designated ‘countable’ range. This is because counting plates with high counts can lead to inaccuracy. (It is widely accepted that the range for countable colonies on a standard agar plate is between 25 and 250 for most bacteria).
      However if both are in your countable range, you would perform the calculation and then determine the mean. For example:
      If the 10^5 plate count was 240 CFU, the final count, after multiplying by 100,000 (or 10^5), would be 24,000,000 CFU/ML
      If the 10^6 plate count was 28 CFU, the final count, after multiplying by 1,000,000 (or 10^6), would be 28,000,000 CFU/ML
      The average of these 2 is 26,000,000 CFU/ML (or 2.6 X 10^7)

  • @speak_up_abu
    @speak_up_abu 3 роки тому

    Thanks a lot

  • @henoknahusenay89
    @henoknahusenay89 6 років тому +1

    why do you need to use extra micro-pipette since you the sample is the same

  • @swarajganiga3127
    @swarajganiga3127 6 років тому +5

    This whole process should be done in aseptic condition....since air also does contain microbes....during the transfer of a loop of culture to the plate aerobic microbes also can enter

    • @aaronramsden1657
      @aaronramsden1657 5 років тому

      Yeah, use a flow hood lol

    • @Dabby3
      @Dabby3 4 роки тому +1

      I think the producers are aware of this fact, but recording is more easy this way.

  • @readyceen
    @readyceen 3 роки тому

    GAS NEMBOK ?

  • @adda_95
    @adda_95 5 років тому

    mam i want learn thing related microbiology and use in biotechnology areas under your guidence can you hier me as a intern?

  • @iamsakc
    @iamsakc 5 років тому

    superb

  • @johnsimple1532
    @johnsimple1532 7 років тому +5

    Woman you are the best 🌹

    • @abzy7216
      @abzy7216 6 років тому

      John Simple your welcome

  • @Ilikepi123
    @Ilikepi123 8 років тому +1

    Thanks!

  • @perdimuhammad3770
    @perdimuhammad3770 4 роки тому

    Ferfect

  • @nangolijoshua1664
    @nangolijoshua1664 3 роки тому

    GOOD ONE PLEASE

  • @SUPERHASITIGER
    @SUPERHASITIGER 8 років тому +1

    you should "vortex" it before you pipette it onto the agar.

  • @jillianlogmao1616
    @jillianlogmao1616 2 роки тому

    2:34

  • @monikaprakashbhai8843
    @monikaprakashbhai8843 6 років тому

    its called a SPC

  • @liongirl2578
    @liongirl2578 6 років тому

    I could not understand anything

  • @shadow.banned
    @shadow.banned 3 роки тому

    So this is why the FDA's sitting on their asses all day...

  • @liongirl2578
    @liongirl2578 6 років тому +5

    Iam a medical student I really really hate microbiology subjects may Allah punish all microbs iam just about to die bcz every day our first lecture is microbiology