Enzyme Purification - Purification chart of an enzyme -specific activity, fold purification , yield

Preparation and construction of a purification chart of the enzyme
specific activity
crude extract
purification factor
yield
volume
Biochemistry
enzymology
MB 333

[@getitdone0010]

Thank you sir watching from USA

[@komal564]

How to calculate overall prorein percentage yield from the table?

[@solakhalid8967]

Plaese... Another question...
Purfication of enzyme from crude extract after determine enzyme activity?

[@microbiology7692]

Means

[@solakhalid8967]

@@microbiology7692 Is enzyme activity determined, then purified, then qualitative activity determined?

[@pranjalghone1597]

Helpful ☺️ thanks 🙏

[@egodindeche917]

Thank you Prof. Pls link to enzyme characterisation will be highly appreciated. Thanks a million.

[@microbiology7692]

Thanks

[@amrutasuryawanshi6604]

Too good.. Thanks sir

[@microbiology7692]

Thanks

[@rockstar8591]

Hiiii

[@Simorg30]

thank you Dr.Prof it was helpful

[@microbiology7692]

No mention and my pleasure

[@alexbhatti394]

thanks you

[@microbiology7692]

Thanks

[@nikitakalal8141]

Can you tell me the conc of protein and conc of unit where did you get...? i mean how to fine this values

[@honeysahibzada9430]

same question

[@komal564]

Sir you didn't reply, how to calculate overall percentage yield of protein?

[@smitshah8074]

thank you sir radhe radhe

[@microbiology7692]

Thanks Smith

[@wonderful3841]

Enzyme activity formula

[@solakhalid8967]

Please... The Yeild for extract crude always equal 1

[@microbiology7692]

Yes.. in terms of percentage 100%

[@solakhalid8967]

@@microbiology7692 what is defenation of yeild and folds?