I learned more about analysing PCR data from this vid than i did scouring the internet for hours, its amazing how people insist on making simple things complex
I'm actually thankful she speaks fast cause in loads of these types of vids they speak soooo slow and have to speed it up to 1.5x or even 2x to not fall asleep. Also, great explanation!
***** This was made for a university assignment and we had to keep it within a 3 min +/- 10 second time limit or we would lose 10%, and qPCR data analysis took longer to explain than we thought, so unfortunately we had to speed up the narration. I can post the script if you would like.
+TARDIStennant It may be unintended, but speaking quickly made this a really good revision tool. Repeating what I had already learnt in the lab slowly made other videos excruciatingly boring. This video is great for those who just need a crash course.
It may be two years too late but now you have the option to slow down video. For instance putting this at 0.75X speed renders it in "normal" speech speed.
I usually get very bored listening to instruction videos when the speaker is talking at a slow or normal pace, so this was actually great for me and encouraged me to stay focused and not fast-forward! Thanks so much, it helped a lot.
+Alexus02 I completely agree that this is too fast. It was made for a uni assignment and unfortunately we had to keep the video at 3 min +/- 15 seconds or we would loose 10% of our mark :( The original narration was about 4min so unfortunately we had to speed up the whole video to get in in the required time, so it doesn't sound as good. Sadly I don't have a copy of the normal speed video but I have included the video script in the description :)
Just had a quick look at a paper about this double delta CT method and understood nothing. Watched this video for three minutes and i have to say i understand the concept of the analysis now. Why do people always write stuffs more complicated than they actually are. Thanks man for the vid!
The work may be good and clear but oh my god is this narration annoying as hell. Good job on your project but the narration speed + the pitch of the voice = not a good combo.
+Bilbo Baggins Thanks! Yeah I completely agree! Unfortunately we had to keep the video at 3 min +/- 15 seconds or we would loose 10% of our mark :( The original narration was about 4min so unfortunately we had to speed up the whole video to get in in the required time, so it doesn't sound as good. Sadly I don't have a copy of the normal speed video but I have included the video script in the description :)
Oh my god so many people just complaining about how speed she talk... The content is AWESOME can't you just be thankfull for that? Thank you so much I got to understand the concept in just 3 minutes after going through half an hour of reading and watching unnecessarily complicated other contents!
I disagree with her assessment concerning relative quantification. Although there is more gene expression of the target gene in dark treatments the ratio seems to be incorrect. Using the Livak method or the deltaCt method you can see that the target gene is expressed at a 32 fold change if the reference gene Ct number is ( for explanation purposes) 2 and the target gene is 10 in light treatments. The reference gene Ct number is 2 and the target gene is 5.
PhD student in Belfast, Ireland here. I have to demonstrate to Undergrads the basics of qPCR tomorrow and thought I'd watch a video or 2 in case I missed anything. Glad I stumbled upon your video. Very concise :) Made better by the fact you're a UWA student. I graduated from UWA in 2010. Cheers
Very good content, concise and well explained, in spite of the slightly irritating style of presentation. I thought I was watching an ad for something before I realised it was the technical video.
I have a question, if the mean of my ct value is 27.6. When I plug this number into my standard curve equation to generate DNA concentration, the concentration is like 660, which doesn't line up with my standard curve plot.
good content ,concise and easy to understand ;thank you for posting this ;even though the image is moving and talk is fast ,it can be understandable and much easier to get the grip of qPCR ,Ct and delta Ct comparing with reading pages of theory
+konfolut I completely agree that this is too fast. It was made for a uni assignment and unfortunately we had to keep the video at 3 min +/- 15 seconds or we would loose 10% of our mark :( The original narration was about 4min so unfortunately we had to speed up the whole video to get in in the required time, so it doesn't sound as good. Sadly I don't have a copy of the normal speed video but I have included the video script in the description :)
Very good vidéo ! I was stuck for 1 hours on my book to get the difference between relative and absolute quantification. 4 min on this video and now it s fully understood and I m french. thank you very much
Very good video and explanation. But I feel stressed by the speed-pitched voice. Why not allow 5 minutes for such a topic and keep your voice natural and relaxed?
+Nia S This was made for a university assignment and we had to keep it within a 3 min +/- 10 second time limit or we would lose 10%, and qPCR data analysis took longer to explain than we thought, so unfortunately we had to speed up the narration. The script is posted in the description :)
Really not understandable in terms of speech speed and tone of the voice. Don't udnerstand people saying the opposite, who clearly have a low critical thinking capability.
+yayoo 04 I completely agree that this is too fast. It was made for a uni assignment and unfortunately we had to keep the video at 3 min +/- 15 seconds or we would loose 10% of our mark :( The original narration was about 4min so unfortunately we had to speed up the whole video to get in in the required time, so it doesn't sound as good. Sadly I don't have a copy of the normal speed video but I have included the video script in the description :)
First time I actually find a use for that "playback speed" option.
0.75x makes it perfect.
Omg so much more sense
Watch this video at 0.75 speed. The voice is too fast and background music is unnecessary. The information is good.
Top 10 rappers Eminem is scared to diss
I learned more about analysing PCR data from this vid than i did scouring the internet for hours, its amazing how people insist on making simple things complex
I'm actually thankful she speaks fast cause in loads of these types of vids they speak soooo slow and have to speed it up to 1.5x or even 2x to not fall asleep.
Also, great explanation!
Yes, same here!
The breakdown was a lot more helpful than more "professional" videos. Thanks!
Why in the world are you speaking so fast??
***** This was made for a university assignment and we had to keep it within a 3 min +/- 10 second time limit or we would lose 10%, and qPCR data analysis took longer to explain than we thought, so unfortunately we had to speed up the narration. I can post the script if you would like.
***** I have now added the script to the description :)
TARDIStennant I understand. Thanks for posting the script!
+TARDIStennant It may be unintended, but speaking quickly made this a really good revision tool. Repeating what I had already learnt in the lab slowly made other videos excruciatingly boring. This video is great for those who just need a crash course.
It may be two years too late but now you have the option to slow down video. For instance putting this at 0.75X speed renders it in "normal" speech speed.
I usually get very bored listening to instruction videos when the speaker is talking at a slow or normal pace, so this was actually great for me and encouraged me to stay focused and not fast-forward! Thanks so much, it helped a lot.
Amazing vid, so helpful and much easier to understand than reading the boring papers about qPCR. Thank you!
JESUS CHRIST SLOW DOWN!!!
+Alexus02 I completely agree that this is too fast. It was made for a uni assignment and unfortunately we had to keep the video at 3 min +/- 15 seconds or we would loose 10% of our mark :( The original narration was about 4min so unfortunately we had to speed up the whole video to get in in the required time, so it doesn't sound as good.
Sadly I don't have a copy of the normal speed video but I have included the video script in the description :)
Ya Allah! please slow down 🌹
@@Legendnos just put it at 75% speed
Excellent summary!! precise and straight to the point
Really clear and understandable. Thanks!
Geez it's like these commentators have no idea that they can pause/rewind/slow down the video........
Just had a quick look at a paper about this double delta CT method and understood nothing. Watched this video for three minutes and i have to say i understand the concept of the analysis now. Why do people always write stuffs more complicated than they actually are. Thanks man for the vid!
This is an awesome video, helpful and doesn't make u go to sleep. Welldone, Do more video like this please. :D
The work may be good and clear but oh my god is this narration annoying as hell. Good job on your project but the narration speed + the pitch of the voice = not a good combo.
+Bilbo Baggins Thanks! Yeah I completely agree! Unfortunately we had to keep the video at 3 min +/- 15 seconds or we would loose 10% of our mark :( The original narration was about 4min so unfortunately we had to speed up the whole video to get in in the required time, so it doesn't sound as good.
Sadly I don't have a copy of the normal speed video but I have included the video script in the description :)
TARDIStennant I see. Still contains good content and that's what matters!
+TARDIStennant I'm glad you made it faster, time is money
Agreed, it was like she was in a hurry
Oh my god so many people just complaining about how speed she talk... The content is AWESOME can't you just be thankfull for that? Thank you so much I got to understand the concept in just 3 minutes after going through half an hour of reading and watching unnecessarily complicated other contents!
Here I am at 3:40am... writing up my results of a TaqMan qPCR... while I understand the theory, analyzing the data has escaped me
at 3:00 don't you mean the expression is 1/3 in the dark compared to the light, instead of 3x in the dark?
10 months too late:
The expression is higher in the dark because we got a lower Ct, meaning we needed less cycles to reach a threshold amount.
I disagree with her assessment concerning relative quantification. Although there is more gene expression of the target gene in dark treatments the ratio seems to be incorrect. Using the Livak method or the deltaCt method you can see that the target gene is expressed at a 32 fold change if the reference gene Ct number is ( for explanation purposes) 2 and the target gene is 10 in light treatments. The reference gene Ct number is 2 and the target gene is 5.
PhD student in Belfast, Ireland here. I have to demonstrate to Undergrads the basics of qPCR tomorrow and thought I'd watch a video or 2 in case I missed anything. Glad I stumbled upon your video. Very concise :) Made better by the fact you're a UWA student. I graduated from UWA in 2010. Cheers
thank you! i was confused how to calculate titer from a ct value but this cleared things up
Well done! It makes complete sense!
Very good content, concise and well explained, in spite of the slightly irritating style of presentation. I thought I was watching an ad for something before I realised it was the technical video.
Nice video! Thank you!
Very brief and accurate!
BEst video on qPCR so far
Use video settings > playback speed to change to 0.75 x speed
I have a question, if the mean of my ct value is 27.6. When I plug this number into my standard curve equation to generate DNA concentration, the concentration is like 660, which doesn't line up with my standard curve plot.
Excellent job !
So Helpful! Thank you :)
My God. Why does she speak so fast?
Pls read the video description or my replies to other comments with this question :)
+TARDIStennant Thank you.
Very nice and thorough, but I'd advice you to slow down a bit.
oh gosh hell the animation is imposing effect on my eye and others as well
I wonder how 2 referecnce genes are calculated in the data analysis?
this video is amazing please make more
Great job guys!
nice explanation though speedy. good job. I am dying hard laughing, reading the comments.
The content is good, the speed of the video can be slowed. Thank you
ty for this quick video... only thing I would suggest is a tripod in the future
So clear. Thank you.
I'm pretty sure people can adjust the playback speed manually. Don't worry about speaking too fast, this video was helpful and informative.
0 min: ct = 34, stimulated 30 min: ct = 35, stimulated 60 min: ct = 32, RT- = 33 how do I calculate ?
thank you very much! finally, I get it! :)))
Great video
Very nice explanation..
I found changing the speed to 1.25x to be very helpful. Great video!
shmmm.... too slow. I find 2x speed very helpful
Awesome
good video
Overall, this is a good video. Although you need to slow down the video speed to at least 0.75 in order to understand it better.
cool... a PCR Rap video (H)
omg y the hell the speaker in a so much of hurry ???
Thanks
OMG thank you so much!
good content ,concise and easy to understand ;thank you for posting this ;even though the image is moving and talk is fast ,it can be understandable and much easier to get the grip of qPCR ,Ct and delta Ct comparing with reading pages of theory
It couldn't be faster, could be?
+konfolut I completely agree that this is too fast. It was made for a uni assignment and unfortunately we had to keep the video at 3 min +/- 15 seconds or we would loose 10% of our mark :( The original narration was about 4min so unfortunately we had to speed up the whole video to get in in the required time, so it doesn't sound as good.
Sadly I don't have a copy of the normal speed video but I have included the video script in the description :)
The CT value is it always =15 ?
No
Thank you!!!!!!!!!!
Very good vidéo ! I was stuck for 1 hours on my book to get the difference between relative and absolute quantification. 4 min on this video and now it s fully understood and I m french.
thank you very much
thank you
In setting, change speed to 0.75
Nice explanation but this was way too fast, you need to slow down
a very good job but OMG how fast.....
McLaughlin Brooks
The video is really nice and useful but cutting it made me dizzy
Is someone chasing you ?
Very good video and explanation. But I feel stressed by the speed-pitched voice. Why not allow 5 minutes for such a topic and keep your voice natural and relaxed?
who's running behind her?!
good job but talk too speed !
Very fast with the narrating but overall good job!
your lecture may be helpful but its not understandable due to speed
Curtis Isle
SHe's speaking too fast...USELSS.
I selected 0.5x speed, now she sounds drunk, but it's alright
Watched it all in 0.75x speed
Dedrick Estates
Not bad but since english is not my native language, 0,75 speed is needed to understand^^ but then its good!
Denesik Cliff
good job but you speaking fasssssssssst and without music job will be excellent.
it is way to fast to understand. slow it down!!!!
+Nia S This was made for a university assignment and we had to keep it within a 3 min +/- 10 second time limit or we would lose 10%, and qPCR data analysis took longer to explain than we thought, so unfortunately we had to speed up the narration. The script is posted in the description :)
Melody Parkways
animation gave me a headache and nausea...
Lee Charles Lopez Robert Clark Angela
this video gave me anxiety
Melis s It's gave anxiety too..😂😂😂😂😂😂. She doesn't speak like human being...
Explain slowly. You are too fast😞
wow wow slow down
No body pushes you to speak so fast. Good material though.
The explanation is not bad, but the animation is annoying to watch, it made me feel dizzy and the background music is unnecessary too.
Really not understandable in terms of speech speed and tone of the voice. Don't udnerstand people saying the opposite, who clearly have a low critical thinking capability.
sooooooo fast
+yayoo 04 I completely agree that this is too fast. It was made for a uni assignment and unfortunately we had to keep the video at 3 min +/- 15 seconds or we would loose 10% of our mark :( The original narration was about 4min so unfortunately we had to speed up the whole video to get in in the required time, so it doesn't sound as good.
Sadly I don't have a copy of the normal speed video but I have included the video script in the description :)
You talk so fast and make je do nervous
Sooooo fast
Could you speak any faster
Too fast to follow..
Keira Heights
You speak too fast i couldn't understand all this ...
You talk to quickly, I cannot follow it…
This video is a headache. The visual is no good.
Way too fast...next video
the presentation its so annoying for the eyes
Less amphetamines next time. Your words are too fast and I'll timed