Can you run 100% water with a C18 column?
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- Опубліковано 5 лис 2024
- 🔬 Mastering Your C18 Column: Essential Tips for Chromatographers 🔬
Welcome to our latest video where we dive into the nuances of using a C18 column in chromatography. Whether you're a seasoned chemist or just starting out, this video is packed with valuable insights and tips to help you get the most out of your C18 column.
👉 What's Inside:
The Water Dilemma: Can you run your C18 column with 100% water? We unravel this common question and explain why it's not recommended. Discover the impact of water on the hydrophobic nature of C18 and how it leads to phase collapse, reducing chromatography efficiency.
Recovery Tactics: Accidentally used too much water? No worries! We show you how to bring your column back to life with 100% acetonitrile or methanol.
Optimal Organic Content: Learn the importance of maintaining at least 10% organic content in your C18 column. We discuss the balance between conservative and adventurous approaches and the signs of phase collapse you should watch out for.
Consistent Results: Key takeaways to ensure your C18 column remains in top shape for consistent, reliable chromatography results.
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good cure to learn!