Відео

I am having an issue running Benzoic acid on a Phenomenex Luna C18 column, the peak for the benzoic acid starts eluting fine but then has a really bad tail towards the end affecting my USP tailing, what I don't understand is that my salicylic acid peak is coming out fine as I have both of those components in the same standard solution what can be causing this tailing? I reverse-flushed the column thinking the column was dirty but it didn't help.

Very helpful, is there a way to program a sequence to do all of this automatically and generate a complete report?

I was feeling hopeless until I found this . I sincerely appreciate you for making this to save our motication!! Thank you very much!!

Gerlach Course

Herbert Turnpike

Thank for your video. I have problem with my HPLC agilent. Sometimes it can't turn on the light automatically. I turn on the light from the control panel manually. How can repair it to be automatically. My next question is that after 5-6 samples hplc doesn't give right concentration. For example i inject the same vial two times. First time it gives a lot of chroma grams . Second time give the right concentration. What is the problem. I think its problem of the dedector

Ava Turnpike

I am thinking about to investi in a businness in which I would need to use this kind of system, my quesiton is if you provide protocols and training to use. I have biochemistry background but have no clue how to operate it and I would to like to identify minerals, vitamines and etc. Simple stuff I think.

I like this guy

Koelpin Ports

Carson Glen

Kyler Valleys

Amazing explanation thank you sir

How do I remove the bubbles in Agilent Infinity II 1260 series

Exactly what i was looking for, thanks!

What could be wrong if my inlet total flow falls when the oven temp is ramping up? I have changed the septum.

Sir how to calculate the delay time from detector to fraction collection tube .Could u pls share ur ideas ?

You are a great speaker. Very confident and helpful, thank you

How do I apply to this class ?

Thanks for the great video!

I'm currently working on a method where a pair of hydrophilic peaks elutes in less than 5 minutes, while another pair elutes about 15 minutes later in isocratic mode. When I use gradient methods (~50 to 70% ACN), the baseline drift increases significantly. What is the best way to run a gradient in these methods? Should I implement a steep gradient change in less than a minute or use a smoother gradient (over a longer period) ?

Sir, how does the molecule come.down when we place positive charge repeller .

Sir, could u pls explain about mass tuning in one video.

Thats very great,thanks for these useful points,could you please explain the thermostat and autosampler parts too?how can i understand the effeciency of these two parts

Is there any good method for erythropoietin in albumin serum ?

wow thanks!

I like you! You're very practical

Sir can u please tell me... Which type of standards used for the Hplc of The sample of TSB broth inoculated with bacterial strains for growth with crude oil?

How i can learn abt calibration, is it available for gcms

Dear sir you explained well..

this Axion guy gets my vote for having fab' knowledge at the tip of his tongue

I’ve read that acetonitrile can have amine impurities and form deposits in pump components over time. Is there a procedure for flushing the system to remove/prevent this?

Great content! Thank u so much for sharing this!!

Great explanation! This is critical when transferring methods into a lab with different brand and models

How to deal with negative peaks on refractive index?

Thank you sir for clearing my doubts 😊

Thanks so much 😊

how does it work if using an internal standard and using their ratios?

Very pocket friendly PI... fellows from this lab will be very lucky

HI Lee indeed inspiring,

Iam facing issue in gcms as it shows vial out of range.hiw can I fix it

What HPLC machine is good enough to check Thc potency?

Can u pls explain how to setup peak purity and threshold in spectra option in agilent

I wish I could work under u and learn .Ur videos r really informative and interesting

Couod u please explain how to set the peak purity and threshold in agilent

I wish I could work under u and learn

What about highly acidic or basic samples? Will that affect the C18 ligands of a column. I work with human serum and plasma that are treated with acid, precipitated and the supernate is injected so not the same as the mobile phase.

Could u pls explain how to setup peak purity setup in spectra option in agilent

wow how excellent video ! this video was very helpful for me, but i want to know amount of sample using calibration curve you described

good cure to learn!