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Axion Labs & Training Institute
United States
Приєднався 8 січ 2016
Axion Training Institute provides hands-on, in-depth training courses for chemists from around the world to share their passion for learning and elevate their analytical skills to make a difference in the modern world. Axion brings you the best in class teachers and techniques, as a part of a world-class experience combining chemistry and community like you’ve never seen before.
For over 30 years, Axion has provided specialized training to every major pharmaceutical, chemical, and petroleum company in the US.
For over 30 years, Axion has provided specialized training to every major pharmaceutical, chemical, and petroleum company in the US.
How does mass spec work?
Watch the full webinar on Axion Lite - Free HPLC and GC training.
courses.axionlabs.com/course/axion-lite?previouspage=allcourses&isenrolled=no#/sales?ticketId=3283437000000185030
Subscribe for more insights into chromatography techniques and applications.
🔗 Follow Us:
Website: axionlabs.com/
Facebook: axionlabs
LinkedIn: www.linkedin.com/company/axion-labs/
#analyticalchemistry #hplc #Chromatography #LabTechniques #ScientificResearch #Chemistry #labtips
Axion offers hands-on HPLC (High-performance liquid chromatography or high-pressure liquid chromatography) and GC (gas chromatography) training courses. Gain advanced HPLC & GC skills with our 5 day hands-on LC/GC bootcamp. If you can't make it to Chicago choose one of our online courses. After taking the LC/GC bootcamp further advance your skills by taking the advanced topics courses.
courses.axionlabs.com/course/axion-lite?previouspage=allcourses&isenrolled=no#/sales?ticketId=3283437000000185030
Subscribe for more insights into chromatography techniques and applications.
🔗 Follow Us:
Website: axionlabs.com/
Facebook: axionlabs
LinkedIn: www.linkedin.com/company/axion-labs/
#analyticalchemistry #hplc #Chromatography #LabTechniques #ScientificResearch #Chemistry #labtips
Axion offers hands-on HPLC (High-performance liquid chromatography or high-pressure liquid chromatography) and GC (gas chromatography) training courses. Gain advanced HPLC & GC skills with our 5 day hands-on LC/GC bootcamp. If you can't make it to Chicago choose one of our online courses. After taking the LC/GC bootcamp further advance your skills by taking the advanced topics courses.
Переглядів: 318
Відео
What's unique about Axion courses?
Переглядів 4076 місяців тому
See what an Axion course is like. Subscribe for more insights into chromatography techniques and applications. 🔗 Follow Us: Website: axionlabs.com/ Facebook: axionlabs LinkedIn: www.linkedin.com/company/axion-labs/ #analyticalchemistry #hplc #Chromatography #LabTechniques #ScientificResearch #Chemistry #labtips Axion offers hands-on HPLC (High-performance liquid chromatography or h...
Everything you need to know about HPLC flow rates
Переглядів 7646 місяців тому
HPLC flow rates very depending on the column diameter. In this video we talk about columns ranging from 2.1 to 50 millimeter ID. Subscribe for more insights into chromatography techniques and applications. 🔗 Follow Us: Website: axionlabs.com/ Facebook: axionlabs LinkedIn: www.linkedin.com/company/axion-labs/ #analyticalchemistry #hplc #Chromatography #LabTechniques #ScientificResea...
When to use a gradient in HPLC?
Переглядів 1,1 тис.6 місяців тому
How do you know when you should use an gradient elution instead of isocratic elution? In this exploration of gradients in chromatography, we unpack the pivotal question: when is a gradient necessary? The heart of the matter lies in the hydrophobic similarities of the molecules being analyzed. If they share similar hydrophobicities, a gradient might not be needed, as indicated by a scouting run ...
Monomeric vs Polymeric C18 Columns
Переглядів 2627 місяців тому
In this video, we dive into the world of chromatography to understand the difference between monomeric and polymeric C18 columns. We break down the technical jargon to explain how these columns work and their significance in chromatographic separations. From the basics to practical applications, join us as we unravel the mysteries behind these essential tools in the lab. Subscribe for more insi...
Can I run an HPLC gradient from 0 to 100%?
Переглядів 4067 місяців тому
For related videos watch: Can you run 100% water with a C18 column? ua-cam.com/video/xwMYDUYPJR0/v-deo.html HPLC Problems: Very polar molecules. ua-cam.com/video/HZV5B_p6OeM/v-deo.html
What is void volume, void time, t0 and solvent front?
Переглядів 6967 місяців тому
What is void volume, void time, t0 and solvent front?
HPLC Tips: How to calculate void volume
Переглядів 1,5 тис.7 місяців тому
In this video we delve into the critical aspect of calculating void volume in High Performance Liquid Chromatography (HPLC). Understanding the void volume, or the space within the column not occupied by the stationary phase, is essential for precise chromatographic analysis. We start by introducing T0 measurement-how to determine the time at which solutes pass through the column without interac...
Can you run 100% water with a C18 column?
Переглядів 7258 місяців тому
🔬 Mastering Your C18 Column: Essential Tips for Chromatographers 🔬 Welcome to our latest video where we dive into the nuances of using a C18 column in chromatography. Whether you're a seasoned chemist or just starting out, this video is packed with valuable insights and tips to help you get the most out of your C18 column. 👉 What's Inside: The Water Dilemma: Can you run your C18 column with 100...
HPLC problems with very polar molecules.
Переглядів 4748 місяців тому
🔬 Welcome to the world of HPLC columns, specifically focusing on the 'Polar Embedded Phase' technology! 🔍 In this video, we unravel the intricacies of these highly efficient columns. If you're a scientist, researcher, or anyone interested in analytical chemistry, this video is for you! 🌊 What Are Polar Embedded Phases? 🌊 Discover the unique 'Polar Embedded Phase' columns in HPLC technology. We ...
Do I need to use an HPLC Guard Column?
Переглядів 7009 місяців тому
In this insightful video we focus on the use of guard columns. We answer these questions: What's the deal with the guard column? Do I need one? Is it required? What's it for? 🔬 What You'll Learn: The fundamental purpose of a guard column in chromatography. How to decide if a guard column is necessary for your specific application. Tips for maintaining your analytical column and extending its li...
Best solvent to dissolve HPLC samples
Переглядів 84910 місяців тому
🔬 Welcome to our latest educational video on chromatography! In this comprehensive guide, we delve into the critical aspect of selecting the perfect sample solvent for effective chromatography. Whether you're a seasoned chemist or a student just starting out, this video offers valuable insights for everyone. 🌟 What's Inside: Impact of Sample Solvent on Chromatography: We start by exploring how ...
How to test a LC column?
Переглядів 53210 місяців тому
In this video, we dive deep into the best ways to test an LC column's performance to ensure optimal results. We'll explore different methods for testing columns, including using specific probes like dimethyl aniline and quinizarin to check for acidity and metal content. However, we emphasize a more practical approach: using your daily standard samples to evaluate column efficiency and peak shap...
How to clean a C18 column?
Переглядів 4,5 тис.11 місяців тому
🔬 In this video guide, we explore the necessary techniques for maintaining the efficiency of your C18 column in chromatography. Our expert tips help identify column issues and offer practical cleaning solutions. 🌐 Highlights of the Article: Identifying Column Issues: Learn how to detect and prevent permanent damages to your HPLC column. Dealing with Dirty or Clogged Frits: Discover the reasons ...
What's the difference between HPLC & UPLC?
Переглядів 2,6 тис.11 місяців тому
What's the difference between HPLC & UPLC?
Ghost Peaks in HPLC - 5 common sources
Переглядів 2,5 тис.Рік тому
Ghost Peaks in HPLC - 5 common sources
How long does it take for a column to equilibrate?
Переглядів 1,3 тис.Рік тому
How long does it take for a column to equilibrate?
HPLC - Negative Peaks and Baseline Drift
Переглядів 6 тис.Рік тому
HPLC - Negative Peaks and Baseline Drift
It doesn't matter which brand GC or HPLC you use.
Переглядів 540Рік тому
It doesn't matter which brand GC or HPLC you use.
Hone Your Chromatography Skills at Our Chromatography Happy Place
Переглядів 282Рік тому
Hone Your Chromatography Skills at Our Chromatography Happy Place
HPLC Troubleshooting: What is causing baseline noise?
Переглядів 13 тис.Рік тому
HPLC Troubleshooting: What is causing baseline noise?
# 1 Most Common Problem in HPLC: Air Bubble in the Pump
Переглядів 28 тис.Рік тому
# 1 Most Common Problem in HPLC: Air Bubble in the Pump
I am thinking about to investi in a businness in which I would need to use this kind of system, my quesiton is if you provide protocols and training to use. I have biochemistry background but have no clue how to operate it and I would to like to identify minerals, vitamines and etc. Simple stuff I think.
I like this guy
Koelpin Ports
Carson Glen
Kyler Valleys
Amazing explanation thank you sir
You are welcome
How do I remove the bubbles in Agilent Infinity II 1260 series
Exactly what i was looking for, thanks!
Glad I could help!
What could be wrong if my inlet total flow falls when the oven temp is ramping up? I have changed the septum.
Sir how to calculate the delay time from detector to fraction collection tube .Could u pls share ur ideas ?
You are a great speaker. Very confident and helpful, thank you
Wow, thank you!
How do I apply to this class ?
Go to axionlabs.com/lc-gc-bootcamp/. This is the hands-on class. We also offer on-demand courses.
Thanks for the great video!
My pleasure!
I'm currently working on a method where a pair of hydrophilic peaks elutes in less than 5 minutes, while another pair elutes about 15 minutes later in isocratic mode. When I use gradient methods (~50 to 70% ACN), the baseline drift increases significantly. What is the best way to run a gradient in these methods? Should I implement a steep gradient change in less than a minute or use a smoother gradient (over a longer period) ?
Sir, how does the molecule come.down when we place positive charge repeller .
Sir, could u pls explain about mass tuning in one video.
Thats very great,thanks for these useful points,could you please explain the thermostat and autosampler parts too?how can i understand the effeciency of these two parts
Is there any good method for erythropoietin in albumin serum ?
wow thanks!
Welcome!
I like you! You're very practical
I appreciate that!
Sir can u please tell me... Which type of standards used for the Hplc of The sample of TSB broth inoculated with bacterial strains for growth with crude oil?
How i can learn abt calibration, is it available for gcms
Dear sir you explained well..
Thank you
this Axion guy gets my vote for having fab' knowledge at the tip of his tongue
I’ve read that acetonitrile can have amine impurities and form deposits in pump components over time. Is there a procedure for flushing the system to remove/prevent this?
Great content! Thank u so much for sharing this!!
My pleasure!
Great explanation! This is critical when transferring methods into a lab with different brand and models
Yes, exactly
How to deal with negative peaks on refractive index?
Thank you sir for clearing my doubts 😊
Thanks so much 😊
You're welcome 😊
how does it work if using an internal standard and using their ratios?
Very pocket friendly PI... fellows from this lab will be very lucky
HI Lee indeed inspiring,
Glad you think so!
Iam facing issue in gcms as it shows vial out of range.hiw can I fix it
What HPLC machine is good enough to check Thc potency?
The best right now are waters arcs hplc amd waters acuity uplc
Can u pls explain how to setup peak purity and threshold in spectra option in agilent
I wish I could work under u and learn .Ur videos r really informative and interesting
Couod u please explain how to set the peak purity and threshold in agilent
I wish I could work under u and learn
What about highly acidic or basic samples? Will that affect the C18 ligands of a column. I work with human serum and plasma that are treated with acid, precipitated and the supernate is injected so not the same as the mobile phase.
Could u pls explain how to setup peak purity setup in spectra option in agilent
wow how excellent video ! this video was very helpful for me, but i want to know amount of sample using calibration curve you described
good cure to learn!
Thank you soo much explaining and making such informatve video.Looking for more such videos .Can you please do a video on peak purity and independent column compartment port switching in agilent in detail
Excellent explanation, awesome video
Glad you liked it!
Thanks for this knowledge but I have a question Sir. Does pH also affect resolution and how?
Can u explain how to check whether uv lamp.intensity has reduced or.not?
I find this video and channel very useful and informative.Super and amazing
Thank you so much 🙂
Amazing .where is ur lab ?
It's in Chicago. Check out our lab here axionlabs.com/lc-gc-bootcamp/
when there is the need of new claibration table then a option of then this table would be deleted??