Hi, I am a great fan of yours as you are doing an awesome job and your videos really help me a lot. But still I have to make this comment so that a misunderstanding does not take place. In the video at 8:28 you said by mistake that ATP converts luciferin into luciferase which is not true. Rather ATP converts luciferin into oxyluciferin that gives luminescence in presence of luciferase enzyme. I know you must have a clear idea about that and you probably had a slip of tongue (because later you only said from luciferin, light is produced with the help of luciferase). So, if you could kindly mention the correct information in a pinned comment or in the description then I think no further confusion will take place. Cheers! And keep teaching us!
@@MuhammadIslamag adenosine phosphosulfate, as it is a substrate and the enzyme that converts these ppi and substrate into ATP is ATP sulfurylase. Thank you
Thumbs up to the instructor. Keep the good work. Bardhan, I appreciate and commend your mannerism of correction. Those are the values proper education instills in our minds. I offer my salutations to all .. One of your kind from Nigeria
By far the most simplest video of pyrosequencing I've possibly ever seen. You make us students understand such complicated topics with such accuracy and ease. Thankyou so much. Keep Uploading Content Sir. You are legit a blessing to the science community
When you said "if there is no thymine, then adenine will not attach and light will not be produced" I almost fell in love with you, you've saved my day. I am forever grateful. Keep doing your amazing work and congrats for the studying you have done in order to reach this level of understanding.
Thank you so much bro. I live in Korea and I am a high school student studying to get admission to Seoul Medical School. Your lectures and tutorials are so helpful bro. Take care man, take care.
@@shomusbiologyofficial you have no idea but u have been helping me a lot with my exams. The clear teaching with daigramatic representations.. God bless. I'm. Sure a lot feel the same too.
Hi brother you are an angel tq for your dedicated work. You are blessed with the talent of making even difficult topics understand in a easy way and it is registered in our mind. I pray for your health wealth and happiness. Tq so much
Thanku shomu sir for ur valuable and truly knowledgeble lectures I love ur all lecture series...and they are worth attending!!! GOD BLESS UH!! Always be happy and teach us as u r teaching..always😍😍🥰 so much thanku from my side
@@shomusbiologyofficial thank you sir...plz teach us always like that I am having my Icar net exam in june I am following just ur videos lectures😁😁 only they r best!! For prep
Sir ,when u say if u like the video then click the like option then i think that how anyone cannot like your teaching video. If i forget to click the like option then it does nt mean at all that i dont like. Thank u sir bundle of thanks sir.
This is very nice. Great! I wanted to request for targeted next gene sequencing. please make videos on this for better understanding, your explanation is too good. Thank you so much for making us to understand the pyrosequencing.
@@shomusbiologyofficial I have been like following your channel since long.. every time books and articles get confusing I know where to go. Seriously thanks!
Shomu da very informative video. There is one slight mistake : APS is adenosine 5' phosphosulphate. I have one doubt what is the use of adenosine alpha thiophosphate ? Btw great video Ur follower from KOLKATA ♥️♥️
Thank you soo much sir for helping us out to clear our doubts.Sir in the video you have by mistakenly told the fullform of APS as Amonium persulfate but it should be( Adenosine 5' -phosphate sulfate )
Thank you Suman sir. It was nicely explained. In this regard I would like to clarify that here APS is Adenosine-5'-phosphosulfate, not ammonium persulfate. Hence it reacts with PPi to produce ATP. However your video is very helpful.
Very well explained. I have query : In a well we have multiple fragments (having varied sequence), then how can we detect the sequencing because at the same time nucleotides are added at the multiple fragments ?
Well explained though i have a question: in other websites I saw that there is a step where we do PCR that you are not mentioning, when do we do the PCR? before the fragmentation of the DNA sequence of after it ?
+Shomu's Biology Thank you so much. About the PCR since you didn't mention it, is it a falcutatif step? can we do pyrosequencing without PCR or is it obligatory to amplify DNA fragments ?
Hi sir am big fan of yours because of your outstanding teaching skills.... but one thing here I want to say that, I think here APS means ADENOSINE PHOSPHOSULPHATE not ammonium per sulphate.
Dear sir, thank very much for your nice and informative lectures, we have a request if you could provide a lecture about RAD sequencing. it will be your kindness.
What determines the sequence of the fragments after the genomic DNA is fragmented and attached with the adapters. The original sequence is maintained by what means? The fragments may not bind to the beads sequentially,.. thanks for the videos
Arent adaptors added to both ends of the DNA?? And how can you break a double stranded DNA to a single stranded DNA?? and upon breaking the strand, how will you seperate the template and the complementary strand?? please answer this at the earliest
Hello, it's a very interesting video, I have some questions, How are the percentages of methylation obtained by the equipment generated? that is, how are they generated? How do you get that percentage? I have looked for this information but I have only found the basis of the chemical reaction but not of obtaining the percentage, I hope you can help me solve my questions, thank you very much
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Hi, I am a great fan of yours as you are doing an awesome job and your videos really help me a lot. But still I have to make this comment so that a misunderstanding does not take place. In the video at 8:28 you said by mistake that ATP converts luciferin into luciferase which is not true. Rather ATP converts luciferin into oxyluciferin that gives luminescence in presence of luciferase enzyme. I know you must have a clear idea about that and you probably had a slip of tongue (because later you only said from luciferin, light is produced with the help of luciferase). So, if you could kindly mention the correct information in a pinned comment or in the description then I think no further confusion will take place. Cheers! And keep teaching us!
precisely!!
Thanku sir
Another mistake APS is Adenosine phosphosulfurase
@@MuhammadIslamag adenosine phosphosulfate, as it is a substrate and the enzyme that converts these ppi and substrate into ATP is ATP sulfurylase.
Thank you
Thumbs up to the instructor. Keep the good work. Bardhan, I appreciate and commend your mannerism of correction. Those are the values proper education instills in our minds.
I offer my salutations to all .. One of your kind from Nigeria
By far the most simplest video of pyrosequencing I've possibly ever seen. You make us students understand such complicated topics with such accuracy and ease. Thankyou so much. Keep Uploading Content Sir. You are legit a blessing to the science community
You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
When you said "if there is no thymine, then adenine will not attach and light will not be produced" I almost fell in love with you, you've saved my day. I am forever grateful. Keep doing your amazing work and congrats for the studying you have done in order to reach this level of understanding.
Thank you so much for appreciating my efforts
Watching this video 4 years later in 2020 is a bliss. Finally was able to grasp the idea of Pyrosequencing in probably the simplest way possible.
Thank you so much for appreciating my efforts
We are blessed to attend such amazing lectures and clarify our concepts 😃👍
You're welcome. Glad to hear that you're getting benefit from my lectures
I just got 30/30 votation in nanobiotechnology exam because of your videos...first person i tought was you...thank you my friend.
Glad to hear that you're getting benefit from my lectures
Thank you so much bro. I live in Korea and I am a high school student studying to get admission to Seoul Medical School. Your lectures and tutorials are so helpful bro. Take care man, take care.
Did you make it into the medical school?
Really useful and simple explanations. Thank you and please keep uploading content! Greetings from Costa Rica
Thank you so much for appreciating my efforts
U will win many hearts of students. Shomu bhaiya ur amazing. I hope ur channel grows a lot within this year.
Thank you so much for appreciating my efforts
@@shomusbiologyofficial you have no idea but u have been helping me a lot with my exams. The clear teaching with daigramatic representations.. God bless. I'm. Sure a lot feel the same too.
I AM A DOCTOR ….I LOVE YOUR VDOS….KEEP GROWING……GOD BLESS YOU
Thank you doctor.
Thank you sooo so so so so so so so much you really saved me .. thank u from the deep of my heart 🥺🥺🥺🌍🌍🇩🇿
You're welcome. Glad you like it
Thank you!!! You are the only one who was able to explain this to me!
Glad to hear that you are getting benefit from the videos
Thank you so much sir for such concept clearing videos!! blessed to have this channel.
You're welcome. Glad you like it
APS is adenosine 5' phosphosulfate, not ammonium persulphate.
@@sinthy8447. does pointing out the mistake of someone is bad????
thank u brother
@@sinthy8447 What is wrong in pointing out mistakes. What is even wrong in making mistakes! It is just human error.
@@shubhagatasarkar exactly!
@@sinthy8447 chup
Your videos make my study very easy👍 good job and more knowledge and wisdom to you
You're welcome
these lectures never disappoint..🎉😊
Thank you so much for appreciating my efforts
You tha man Shomu. Thanks for helping out.
+H2OGOAu you're welcome
Thanku sir for helping me
Hi brother you are an angel tq for your dedicated work. You are blessed with the talent of making even difficult topics understand in a easy way and it is registered in our mind. I pray for your health wealth and happiness. Tq so much
You are a legend, explain everything so clearly! Thank you so much, you are saving me in my exam!
I understanded alot of topics from your videos, big thank you Dr.Shomu's.
Glad to help you out
Thanku shomu sir for ur valuable and truly knowledgeble lectures I love ur all lecture series...and they are worth attending!!! GOD BLESS UH!! Always be happy and teach us as u r teaching..always😍😍🥰 so much thanku from my side
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
@@shomusbiologyofficial thank you sir...plz teach us always like that I am having my Icar net exam in june I am following just ur videos lectures😁😁 only they r best!! For prep
Thank you so much for your explanation it's really clear and it helps me a lot
Thank you so much for appreciating my efforts
Sir ,when u say if u like the video then click the like option then i think that how anyone cannot like your teaching video. If i forget to click the like option then it does nt mean at all that i dont like.
Thank u sir bundle of thanks sir.
Thank you for appreciating my efforts
Appreciate much for the clear and interesting explanation for pyrosequencing.Its helps more on my understanding!
+tai huili thank you. Glad it helped
Thank you so much MR Shomu. i gain lots info..very clear and easy to understand..Done subscribed.
You're welcome. Glad to hear that you're getting benefit from my lectures. Thank you
last day preparation for my exam >>> goddamn useful'
Thank you. Nice to know that my lectures are helping you out
Really good teaching holding you have sir I can understand difficult concepts also thank you sir.
Thank you so much for appreciating my efforts
well done May Allah ease every thing for you as you are doing for me
This is very nice. Great!
I wanted to request for targeted next gene sequencing. please make videos on this for better understanding, your explanation is too good. Thank you so much for making us to understand the pyrosequencing.
Thank you for appreciating my efforts
❤you are a true legend sir! 🙌❤
Thank you
Amazing explanation.. finally I get it!
Glad to hear that you are getting benefit from the videos
@@shomusbiologyofficial I have been like following your channel since long.. every time books and articles get confusing I know where to go. Seriously thanks!
Wow u r amazing sir i love your vedios and how u explain u so better than our prof
Thnk u so much
God pless u
Thankyou so much sir 🙏😊 great explanation
Thank you so much for appreciating my efforts
Shomu da very informative video. There is one slight mistake : APS is adenosine 5' phosphosulphate. I have one doubt what is the use of adenosine alpha thiophosphate ?
Btw great video
Ur follower from KOLKATA ♥️♥️
U r the best sir ur videos are just awsum😇😇
Thank you
thanks you very much!!!! can u explain why do we need an adaptor please ?
Thank you so much you saved my life
You're welcome
Thank you soo much sir for helping us out to clear our doubts.Sir in the video you have by mistakenly told the fullform of APS as Amonium persulfate but it should be( Adenosine 5' -phosphate sulfate )
Yes sorry
Thank you for the lecture🤯🤯👏👏
You're welcome
best and the easiest I've seen so far
Thank you
THANKYOU so much youhave helped me to understand prosequencing very well
You're welcome
Sir this type of video gives more understanding rather than animated one...
Thank you. Glad you liked my lectures
Thankyou sitr💙
You're welcome
please make video on genome finishing - gaps and their resolution
would be very helpful! ❤
You're welcome
You are doing great job bro. APS is Adenosine phosphosulfurase
Yes it is
love you bhai ..u are really great
You're welcome
thanks for that wonderful explaination
You're welcome
Thank you very much .It is very clear explanation.
+mina sattari thank you. Glad you liked my lectures
thank you very much, it is simple and perfect.
You're welcome
Recommmended to every interested Student. 😍😍😍
You're welcome. Glad to hear that you're getting benefit from my lectures
Thank you Suman sir. It was nicely explained. In this regard I would like to clarify that here APS is Adenosine-5'-phosphosulfate, not ammonium persulfate. Hence it reacts with PPi to produce ATP. However your video is very helpful.
+Subhajit Chatterjee yes. It is adenosine 5 phosphosulfate. I put that correction in the annotation didn't i ??
Actually I didn't check that. However it's good have your lectures and they're really very helpful.
+Subhajit Chatterjee Please I need to understant why didn't he mention the PCR ? is it before or after DNA fragmentation
explanation is awesome
+saranya krishnan thank you.
very clear explanation. thank you very much
Brilliant ❤️
Very well explained👌
You're welcome
Really love you Dada...
Thank you
Very well explained.
I have query : In a well we have multiple fragments (having varied sequence), then how can we detect the sequencing because at the same time nucleotides are added at the multiple fragments ?
This is amazing!!! One question, how can we know if it’s a heterozygous?
At one time we add many nucleotides of same type or one only in the wells?
@Shomu da, apni na thakle exam gulo te udhhar hote partam na kokhonoi! Happy Teacher's Day, Sir! Bhalo thakben! :)
Thank you so much for appreciating my efforts
Thank you!❤
You're welcome
thanks sir, helps a lot
You're welcome
wonderful 🥰🥰🥰🥰
love u so much 😍😍
Thank you
Thank u soo much sir. It means a lot...
You're welcome
how does the light allow you to determine which base was added
amazing you really did a great job......
APS doesnt stands for ammonium per sulphate but it stands for adenosine phospho sulphate. still your lectures are really helpful
This is what learning should be like: fun
Thank you
Sir we won't amplify the DNA fragments in bead first before putting into the well?
Very nice and well explained lecture
Thanks, man 👍.
You're welcome
Good explaination
Thank you
Well explained though i have a question: in other websites I saw that there is a step where we do PCR that you are not mentioning, when do we do the PCR? before the fragmentation of the DNA sequence of after it ?
+Oumaima El Hachimi after fragmentation.
+Shomu's Biology Thank you so much. About the PCR since you didn't mention it, is it a falcutatif step? can we do pyrosequencing without PCR or is it obligatory to amplify DNA fragments ?
I have a question 🤔
Who apyrase is added from the beginning of the procedure and not affected the nucleotide that newly added in each time ????
Thanks! what is the sequencing QiaSeq target amplicon method different from Sanger sequencing method ? which is superior?
Thankyou! Can you make a video on solid sequencing as well? Thanks in advance :)
Sure
Welldone sir....
You're welcome
Hi sir am big fan of yours because of your outstanding teaching skills.... but one thing here I want to say that, I think here APS means ADENOSINE PHOSPHOSULPHATE not ammonium per sulphate.
Adenosine phosphosulfate
thank you very much, it is simple and perfect..
Very well explained ! Only one thing .... APS is Adenosine 5' phosphosulfate !
very convoluted lecture
I need help for bisulfite pyrosequencing for DNA methylation. How pyromark software estimates methylation percentage?
Thank you ❤
You're welcome
Our professor should learn from you
Thank you
hello. really liked thie video. can you please make such a video on Support oligo ligation and detection(SoLiD) technique too? that will be great.
Dear sir, thank very much for your nice and informative lectures, we have a request if you could provide a lecture about RAD sequencing. it will be your kindness.
very well explained..... thanks mate
Perfect👍👍
Thank you
U are the best 👍🏻👍🏻
+Gurkamal kaur thank you. Glad you liked my lectures
What determines the sequence of the fragments after the genomic DNA is fragmented and attached with the adapters. The original sequence is maintained by what means? The fragments may not bind to the beads sequentially,.. thanks for the videos
Arent adaptors added to both ends of the DNA?? And how can you break a double stranded DNA to a single stranded DNA?? and upon breaking the strand, how will you seperate the template and the complementary strand??
please answer this at the earliest
much useful .. thankyou
You're welcome
454 sequencing and pyrosequencing are different? I was always told they are the same.
I think Roche 454 is a comercial name. The biochemical name is pyrosequencing.
454 method is a general procedure to perform pyrosequencing as well as ion torrent sequencing.. ROCHE 454 is a kind of emulsion PCR technique.
You are the best thank you so much
Plz make video for solid sequncing
Sure
Nice explanation sir!
Kindly suggest some good book to study ngs in detail
Hello, it's a very interesting video, I have some questions, How are the percentages of methylation obtained by the equipment generated? that is, how are they generated? How do you get that percentage? I have looked for this information but I have only found the basis of the chemical reaction but not of obtaining the percentage, I hope you can help me solve my questions, thank you very much
Thank you sir
You're welcome
very Clear
You're welcome