series of high quality presentations, I have been doing cytometry for 15 years, this will allow me to explain with precision what I was doing without sometimes knowing why
Thank you for an excellent course. I am new to flow cytometry - how do you ensure that a single stained control / compensation control is brighter than overall sample, if the cell population we are targeting (in a compensation control tube) has low expression of surface marker? I would assume that a fluorophore-conjugated low expression surface marker would not emit high fluorescence signal due to small number of cells expressing said marker, even if the cell suspension is stained with a bright dye such as PE. Thank you for your help.
Hi! This can be a tricky question. First I want to point out that the frequency and intensity of a marker are distinct features and independent from each other. You can have a low frequency population (
![Flow cytometry - Compensation of spectral overlap [WEBINAR]](/img/n.gif)
series of high quality presentations, I have been doing cytometry for 15 years, this will allow me to explain with precision what I was doing without sometimes knowing why
Thank you for an excellent course. I am new to flow cytometry - how do you ensure that a single stained control / compensation control is brighter than overall sample, if the cell population we are targeting (in a compensation control tube) has low expression of surface marker? I would assume that a fluorophore-conjugated low expression surface marker would not emit high fluorescence signal due to small number of cells expressing said marker, even if the cell suspension is stained with a bright dye such as PE. Thank you for your help.
Hi! This can be a tricky question. First I want to point out that the frequency and intensity of a marker are distinct features and independent from each other. You can have a low frequency population (
Bravo!!!!