Thank you for the tutorial! Very helpful. One more question: you stained 1M cells in 100ul buffer flow analysis with around 0.5-1ul antibody. For sorting, how much volume buffer and how much antibody do you use? Could you please give a suggestion? Thanks!
Thank you for your video.I’m analyzing human cultured adipocyte tissue derived cells. These cells are already cultured and passaged several times. To obtain a single cell suspension is it necessary to pass the cell suspension through a cell strainer? I’m worried because of the diameter of the ASCs I might lose the cells
I'm sorry, I'm not familiar with this cell type so I don't think I can advise you. I don't think cell strainers are an absolute requirement, but they work well for cells that are often used in flow cytometry. You'll have to find a method of single cell preparation that is appropriate for your cell type.
Thank you so much for the video! much better training!
Excellent video, Thanks
Amazing for me: a beginner on flow!!!
Great video! So detailed and informative! Thank you!!
Thank you!!
Thank you for the tutorial! Very helpful. One more question: you stained 1M cells in 100ul buffer flow analysis with around 0.5-1ul antibody. For sorting, how much volume buffer and how much antibody do you use? Could you please give a suggestion? Thanks!
Good video and Please can u have more information in flow
Thank you very much! Which topic would you like us to cover next?
@@UChicagoFlow Please upload a detailed video of system calibration , standardisation and laser delay.
Thank you for your video.I’m analyzing human cultured adipocyte tissue derived cells. These cells are already cultured and passaged several times.
To obtain a single cell suspension is it necessary to pass the cell suspension through a cell strainer?
I’m worried because of the diameter of the ASCs I might lose the cells
I'm sorry, I'm not familiar with this cell type so I don't think I can advise you. I don't think cell strainers are an absolute requirement, but they work well for cells that are often used in flow cytometry. You'll have to find a method of single cell preparation that is appropriate for your cell type.
@@UChicagoFlow Thank you for taking your time to reply.
I appreciate it.
I will continue to search for the optimal conditions.