Man, you deserve to earn $150 for every full video watched. I literally took a 2 hours training on Flow Cytometry yesterday ($75 an hour, paid by the lab though) from some super speed instructor who would not wait for me to take notes even and learned literally nothing. As soon as you started explaining what all those windows were, I knew your video is going to be great. This must be the most beautifully explained video for flow cytometry on the internet. Thank you man, I wasted my lab's money yesterday and I am learning from you today. I will watch your next video on compensation too.
Dude I thought of making one of these videos as so many of my friends are asking. You explained it nicely. I can simply share your video if someone asks. By the way you have nicely done it covering each and every point needed for the beginners.
Excellent video. Thank you very much! I am a beginner with Flow Cytometry and have been struggling to find someone to give clear step-by-step explanations. Question: Is there a minimum number of cells per tube that you would recommend in order to have a good experiment? Thanks!
The best tutorial of voltage setting.Wish there were more than 2 colors though. How do you set voltage for like 3 colors. For instance, say you have PE, PerCPcy5.5 and FITC. Would you then look for setting voltage with FITC against PE and PerCPcy5.5?
Thank you so much for this video. I learned from it a lot. This is the best video so far explaining the basics of how to run samples with 2 flourophores. I am a beginner in flow cytometry and I got a lot of info from this video. I have a question, I found that some people do a dot plot for FSC-A Vs FSC-H dot plot, is this plot gives the same info as FSC Vs SC dot plot regarding giving info about doublet and singlet cells?
No, those are 2 separate plots. You start with FSC vs SSC to eliminate debris (usually below the 50K markers on both axes somewhere. Next is to draw FSC-A (X) vs FSC-H (Y): the singlets are all on the diagonal. The doublets stick out to the right on the X-axis and should not be included in your gate. You can set the same for SSC (SSC-A vs SSC-H). Some people use these consecutively to eliminate doublets both according to FSC, and subsequently according to SSC. Cheers!
I have one doubt. Is it needed to link the experiment to any of lysed wash or lysed no wash parameters everytime when ever you are doing compensation with single tubes each colour.
Man, you deserve to earn $150 for every full video watched. I literally took a 2 hours training on Flow Cytometry yesterday ($75 an hour, paid by the lab though) from some super speed instructor who would not wait for me to take notes even and learned literally nothing. As soon as you started explaining what all those windows were, I knew your video is going to be great. This must be the most beautifully explained video for flow cytometry on the internet. Thank you man, I wasted my lab's money yesterday and I am learning from you today. I will watch your next video on compensation too.
This was the clearest explanation I ever get about FACS and flow. It helped me a lot to understand. Thank you very much!
Most interestingly it's a good showcase on how to use FACS Diva software! Sooooo helpful!
Great demonstration!!! Thank you very much for the upload.
This is a mind-blowingly good demonstration. Seriously, mate. Thanks a lot.
Thank you soo very much! I have been searching forba clear cut explanation that isnt just someone explaining the theory.
Amazing demonstration of FACS Diva! Thank you so much for this helpful video!
This is an excellent demonstration
Very good demonstration! Extremely helpful!
The best video for flow cytometry experiment found ever
Thank you for being much more helpful than anyone in my lab or institution. Much appreciated 🙏
this tutorial is much better than the previous training I received, even that trainer speaks my native language and you explain all in English.
Dude I thought of making one of these videos as so many of my friends are asking. You explained it nicely. I can simply share your video if someone asks. By the way you have nicely done it covering each and every point needed for the beginners.
very helpful! Indeed today I just wondered how to adjust the voltage and how to set up p2/p3/p4. Thanks a lot!
Yasss, you did it with this explanation! Thanks a lot!!
This is a great compensation tutorial, amazing work! I wish I would have found this sooner :'c
Excellent demo! Extremely helpful, thanks for sharing this video!
Thank you for such a great video! I am learning flow cytometry and this helps a lot
Thank you very much for the video. It definitely helped to backup what I learned. Would love to see one with compensation.
Excellent presentation. It is clear, concise and engaging. I truly appreciate your willingness to share your expertise.
Lovely presentation for the flow cytometry. It really helped me a lot. Many thanks.
This is fantastic work. Thank you for sharing.
Thank you VERY MUCH! Very hopeful! Please keep up the good work!
clearest explanation！excellent job！ thank you very much。
This is really helpful! Thank you soooo much!!
Very well explained. Thanks for the detailed information.
Great demonstration, thank youu
thank you so much for good demonstration!!! Extremely helpful!!!
Thank you!!! That makes sense for the beginner!
You are a life saver! Thanks!
Very helpful! Thanks so much!
Thank you very much, this is really enlightening.
It really helps. Thank you very much!!
Great Demonstration would you please add more videos on FACS and other cytometers
Thank you very much! that helpes a lot
thank you so much, this is really helpful!
very well explained...thank you so much
Great job, thank you!!!!
Great work,, cheers
Thank you for this!
Thanks a lot,. very helpful!
Very clear video with great detail. Do you have a video of the setup of a more complex experiment? One that would require compensation.
Very helpful! Thank you
Thank you so much. It's very useful!
Thanks a lot, I learnt lots of useful things.
Fantastic video. Thanks
Very useful and clearly explained.
Thank you very much. It was very understandable video.
Very helpful. Thank you very much.
Great, better than our technicians :). Thanks
Many thanks this was great
This was helpful. Thanks a lot :)
Great! Thank you!
Thanks a lot! Very clearly explained...
Thanks bro. This really helped a lot.
so nice explanation , thanks
Excellent video. Thank you very much! I am a beginner with Flow Cytometry and have been struggling to find someone to give clear step-by-step explanations. Question: Is there a minimum number of cells per tube that you would recommend in order to have a good experiment? Thanks!
very easy explanation.Good video
Thank you
Thank you so much
Clear explannation
Thank you!!!!!
THANK YOU
😀Very useful. Thanks,
Many Thanks
The best tutorial of voltage setting.Wish there were more than 2 colors though. How do you set voltage for like 3 colors. For instance, say you have PE, PerCPcy5.5 and FITC. Would you then look for setting voltage with FITC against PE and PerCPcy5.5?
Thank you so much for this video. I learned from it a lot. This is the best video so far explaining the basics of how to run samples with 2 flourophores. I am a beginner in flow cytometry and I got a lot of info from this video. I have a question, I found that some people do a dot plot for FSC-A Vs FSC-H dot plot, is this plot gives the same info as FSC Vs SC dot plot regarding giving info about doublet and singlet cells?
No, those are 2 separate plots. You start with FSC vs SSC to eliminate debris (usually below the 50K markers on both axes somewhere. Next is to draw FSC-A (X) vs FSC-H (Y): the singlets are all on the diagonal. The doublets stick out to the right on the X-axis and should not be included in your gate. You can set the same for SSC (SSC-A vs SSC-H). Some people use these consecutively to eliminate doublets both according to FSC, and subsequently according to SSC. Cheers!
I have one doubt. Is it needed to link the experiment to any of lysed wash or lysed no wash parameters everytime when ever you are doing compensation with single tubes each colour.
When you ran the compensation, did you run Low or High? And also for the cells? Thanks
My institution offers a 2 hour tutorial for what you're showing in half an hour here
Where do we get the user manual for this instrument?
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Hi, may I know the name and background of the presenter? Thank you. This video is so valuable!
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video quality is extremely poor so sad
Very helpful! Thanks!!
Thank you, very helpful
Thankyou. This was super helpful ;)
Thank you so much