Man, you deserve to earn $150 for every full video watched. I literally took a 2 hours training on Flow Cytometry yesterday ($75 an hour, paid by the lab though) from some super speed instructor who would not wait for me to take notes even and learned literally nothing. As soon as you started explaining what all those windows were, I knew your video is going to be great. This must be the most beautifully explained video for flow cytometry on the internet. Thank you man, I wasted my lab's money yesterday and I am learning from you today. I will watch your next video on compensation too.
Very nice video and clearly explained the basic operation of FACSDiva. Though I got trained by our core facility, your video made me less frustrated for learning to use this complex machine and software.
Dude I thought of making one of these videos as so many of my friends are asking. You explained it nicely. I can simply share your video if someone asks. By the way you have nicely done it covering each and every point needed for the beginners.
Excellent video. Thank you very much! I am a beginner with Flow Cytometry and have been struggling to find someone to give clear step-by-step explanations. Question: Is there a minimum number of cells per tube that you would recommend in order to have a good experiment? Thanks!
The best tutorial of voltage setting.Wish there were more than 2 colors though. How do you set voltage for like 3 colors. For instance, say you have PE, PerCPcy5.5 and FITC. Would you then look for setting voltage with FITC against PE and PerCPcy5.5?
Thank you so much for this video. I learned from it a lot. This is the best video so far explaining the basics of how to run samples with 2 flourophores. I am a beginner in flow cytometry and I got a lot of info from this video. I have a question, I found that some people do a dot plot for FSC-A Vs FSC-H dot plot, is this plot gives the same info as FSC Vs SC dot plot regarding giving info about doublet and singlet cells?
No, those are 2 separate plots. You start with FSC vs SSC to eliminate debris (usually below the 50K markers on both axes somewhere. Next is to draw FSC-A (X) vs FSC-H (Y): the singlets are all on the diagonal. The doublets stick out to the right on the X-axis and should not be included in your gate. You can set the same for SSC (SSC-A vs SSC-H). Some people use these consecutively to eliminate doublets both according to FSC, and subsequently according to SSC. Cheers!
I have one doubt. Is it needed to link the experiment to any of lysed wash or lysed no wash parameters everytime when ever you are doing compensation with single tubes each colour.
![Multicolor flow cytometry - Understanding the basics [WEBINAR]](/img/n.gif)
This was the clearest explanation I ever get about FACS and flow. It helped me a lot to understand. Thank you very much!
Most interestingly it's a good showcase on how to use FACS Diva software! Sooooo helpful!
Man, you deserve to earn $150 for every full video watched. I literally took a 2 hours training on Flow Cytometry yesterday ($75 an hour, paid by the lab though) from some super speed instructor who would not wait for me to take notes even and learned literally nothing. As soon as you started explaining what all those windows were, I knew your video is going to be great. This must be the most beautifully explained video for flow cytometry on the internet. Thank you man, I wasted my lab's money yesterday and I am learning from you today. I will watch your next video on compensation too.
This is so far the best video I have seen on FACS DIVA to set an experiment, to set controls, and to resolve different populations
this tutorial is much better than the previous training I received, even that trainer speaks my native language and you explain all in English.
Excellent presentation. It is clear, concise and engaging. I truly appreciate your willingness to share your expertise.
Thank you soo very much! I have been searching forba clear cut explanation that isnt just someone explaining the theory.
This is a mind-blowingly good demonstration. Seriously, mate. Thanks a lot.
Very nice video and clearly explained the basic operation of FACSDiva. Though I got trained by our core facility, your video made me less frustrated for learning to use this complex machine and software.
Dude I thought of making one of these videos as so many of my friends are asking. You explained it nicely. I can simply share your video if someone asks. By the way you have nicely done it covering each and every point needed for the beginners.
I can't say enough, THANK YOU SO VERY MUCH for this!!!!! FANTASTIC video and explanation
The best video for flow cytometry experiment found ever
Thank you for being much more helpful than anyone in my lab or institution. Much appreciated 🙏
Excellent demo! Extremely helpful, thanks for sharing this video!
Lovely presentation for the flow cytometry. It really helped me a lot. Many thanks.
Amazing demonstration of FACS Diva! Thank you so much for this helpful video!
Very nice and detailed explanation
Great demonstration!!! Thank you very much for the upload.
Thank you very much for the video. It definitely helped to backup what I learned. Would love to see one with compensation.
This is an excellent demonstration
thank you so much for good demonstration!!! Extremely helpful!!!
This is a great compensation tutorial, amazing work! I wish I would have found this sooner :'c
Thank you for such a great video! I am learning flow cytometry and this helps a lot
Very good demonstration! Extremely helpful!
very helpful! Indeed today I just wondered how to adjust the voltage and how to set up p2/p3/p4. Thanks a lot!
clearest explanation！excellent job！ thank you very much。
Great, better than our technicians :). Thanks
Thank you very much, this is really enlightening.
Thank you!!! That makes sense for the beginner!
very well explained...thank you so much
Thanks a lot! Very clearly explained...
Very useful and clearly explained.
Thank you VERY MUCH! Very hopeful! Please keep up the good work!
Thanks bro. This really helped a lot.
This is fantastic work. Thank you for sharing.
Yasss, you did it with this explanation! Thanks a lot!!
Thanks a lot, I learnt lots of useful things.
very easy explanation.Good video
You are a life saver! Thanks!
Very well explained. Thanks for the detailed information.
😀Very useful. Thanks,
Fantastic video. Thanks
Excellent video. Thank you very much! I am a beginner with Flow Cytometry and have been struggling to find someone to give clear step-by-step explanations. Question: Is there a minimum number of cells per tube that you would recommend in order to have a good experiment? Thanks!
Thank you very much. It was very understandable video.
Great demonstration, thank youu
Many thanks this was great
It really helps. Thank you very much!!
Great Demonstration would you please add more videos on FACS and other cytometers
thank you so much, this is really helpful!
The best tutorial of voltage setting.Wish there were more than 2 colors though. How do you set voltage for like 3 colors. For instance, say you have PE, PerCPcy5.5 and FITC. Would you then look for setting voltage with FITC against PE and PerCPcy5.5?
Very clear video with great detail. Do you have a video of the setup of a more complex experiment? One that would require compensation.
Thank you very much! that helpes a lot
This is really helpful! Thank you soooo much!!
Clear explannation
Thank you for this!
Very helpful. Thank you very much.
Where do we get the user manual for this instrument?
Thank you so much for this video. I learned from it a lot. This is the best video so far explaining the basics of how to run samples with 2 flourophores. I am a beginner in flow cytometry and I got a lot of info from this video. I have a question, I found that some people do a dot plot for FSC-A Vs FSC-H dot plot, is this plot gives the same info as FSC Vs SC dot plot regarding giving info about doublet and singlet cells?
No, those are 2 separate plots. You start with FSC vs SSC to eliminate debris (usually below the 50K markers on both axes somewhere. Next is to draw FSC-A (X) vs FSC-H (Y): the singlets are all on the diagonal. The doublets stick out to the right on the X-axis and should not be included in your gate. You can set the same for SSC (SSC-A vs SSC-H). Some people use these consecutively to eliminate doublets both according to FSC, and subsequently according to SSC. Cheers!
Many Thanks
Hi, may I know the name and background of the presenter? Thank you. This video is so valuable!
Thank you!!!!!
Thanks a lot,. very helpful!
Thankyou. This was super helpful ;)
Very helpful! Thank you
When you ran the compensation, did you run Low or High? And also for the cells? Thanks
Great job, thank you!!!!
This was helpful. Thanks a lot :)
so nice explanation , thanks
Great work,, cheers
Thank you, very helpful
I have one doubt. Is it needed to link the experiment to any of lysed wash or lysed no wash parameters everytime when ever you are doing compensation with single tubes each colour.
Very helpful! Thanks!!
Thank you so much
Great! Thank you!
Thank you
THANK YOU
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My institution offers a 2 hour tutorial for what you're showing in half an hour here
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video quality is extremely poor so sad
Thank you so much. It's very useful!
Very helpful! Thanks so much!
Thank you so much
Thank you so much